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Genet Mol Res ; 14(3): 9423-36, 2015 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-26345876

RESUMO

Loquat (Eriobotrya japonica Lindl.), which originates from the cooler hill regions of southwestern China, is a typical subtropical evergreen tree. Loquat is one of the most important economic crops in China, but the available genomic information is very limited. Here, we present the first deep transcriptomic analysis of loquat. De novo assembly generated 116,723 contigs and 64,814 unigenes using Illumina sequencing technology. A total of 45,739 unigenes were annotated by Nr, GO, and COG datasets. In addition, we analyzed the gene expression profiles of loquat fruit under low temperature stress and 4017 differential expressed genes were identified. We found that the unigenes involved in the brassinosteroid biosynthesis and phosphatidylinositol signaling systems were upregulated, indicating that they have an important role in the resistance of plants to low temperature. Our results provide an invaluable resource for identification of specific genes and proteins involved in loquat development and response to low temperatures.


Assuntos
Temperatura Baixa , Eriobotrya/genética , Regulação da Expressão Gênica de Plantas , Estresse Fisiológico , Transcriptoma , Biologia Computacional , Eriobotrya/metabolismo , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Redes e Vias Metabólicas , Anotação de Sequência Molecular , Fitosteróis/metabolismo , Transdução de Sinais
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