Students' learning as the focus for shared involvement between universities and clinical practice: a didactic model for postgraduate degree projects.

In an academic programme, completion of a postgraduate degree project could be a significant means of promoting student learning in evidence- and experience-based practice. In specialist nursing education, which through the European Bologna process would be raised to the master's level, there is no tradition of including a postgraduate degree project. The aim was to develop a didactic model for specialist nursing students' postgraduate degree projects within the second cycle of higher education (master's level) and with a specific focus on nurturing shared involvement between universities and healthcare settings. This study embodies a participatory action research and theory-generating design founded on empirically practical try-outs. The 3-year project included five Swedish universities and related healthcare settings. A series of activities was performed and a number of data sources secured. Constant comparative analysis was applied. A didactic model is proposed for postgraduate degree projects in specialist nursing education aimed at nurturing shared involvement between universities and healthcare settings. The focus of the model is student learning in order to prepare the students for participation as specialist nurses in clinical knowledge development. The model is developed for the specialist nursing education, but it is general and could be applicable to various education programmes.

Annoyance and health symptoms and their influencing factors: a population-based air pollution intervention study.

OBJECTIVES: Interventions for reducing air pollution are important means for improving public health. The role of psychological factors in understanding annoyance and health symptoms due to air pollution is limited and further investigation is required. This study aimed to investigate the effects of an intervention to reduce air pollution (predominantly dust and soot) with respect to perceived pollution, risk perception, annoyance and health symptoms. Another objective was to test a model that describes inter-relations between air pollution, perceived pollution, health risk perception, annoyance and health symptoms. STUDY DESIGN: An interventional, population-based questionnaire study. METHODS: Surveys were performed before (pre-test) and after (post-test) closure of a sinter plant. Instead, pellets were shipped to the community's harbour for steel production. Individuals in the community aged 18-75 years were selected at random for participation in the pre-test (n=738; 74% of the sample participated) and post-test (n=684; 68% of the sample participated). The two samples were representative of the populations at the two points in time, and thus not identical. RESULTS: After the sinter plant was closed, the environment was perceived as being less dusty, the residents were more positive in their risk perception, and they reported less annoyance due to dust, soot and odorous substances. No difference was found for health symptoms between the pre-test and the post-test. Based on path analyses, a model is proposed of inter-relations between air pollution, perceived pollution, health risk perception, annoyance and health symptoms. CONCLUSION: The intervention was successful with respect to perceived dust and soot pollution; to annoyance attributed to dust, soot and odorous substances; and to risk perception. The path analyses suggest that perceived pollution and health risk perception play important roles in understanding and predicting environmentally induced annoyance and health symptoms.

The Scandinavian Odor-Identification Test: development, reliability, validity and normative data.

The Scandinavian Odor-Identification Test (SOIT) was developed to address the need for a culturally valid odor-identification test for clinical use on the Scandinavian population that (i) has good ability to generalize performance to olfactory status, (ii) assesses olfactory and trigeminal function separately, (iii) requires only limited cognitive demands, (iv) has normative data and good sensitivity and specificity, and (v) is fast, easy to administer and inexpensive. Sixteen odorous test stimuli were selected that were relatively identifiable, familiar, strong in intensity and pleasant according to healthy participants. Four response alternatives were then selected for each test stimulus based on a confusion matrix of identification rates obtained from healthy participants, in a manner that controlled for task difficulty. Results on the SOIT from healthy persons and hyposmic patients showed satisfactory test-retest reliability, split-half reliability and validity. Cut-off scores for olfactory diagnosis (normosmia, hyposmia, anosmia) based on normative data obtained from 171 healthy persons showed a satisfactory sensitivity and specificity of the SOIT. Assessment of 22 anosmic patients with the SOIT revealed that three of the test stimuli were significant trigeminal stimulants.

Depolarization of the neuronal membrane caused by cotransport of taurine and sodium.

C 1300 neuroblastoma cells were cultured and used to study the effect of sodium dependent taurine transport on the membrane potential. Measuring net accumulation of taurine and the depolarization caused by externally applied taurine, we found both processes become active at an external concentration of taurine of 1 mM or more. Net accumulation had Km of 13 mM and a Vmax of 126 nmol x mg of protein-1 x min-1. The taurine induced depolarization of the neuroblastoma cell was parallelled by a 25 per cent decrease in its membrane impedance. The transport of taurine, the depolarization caused by taurine and the effect of taurine on the membrane impedance, all, had a similar dependence on the external sodium concentration. Our results on the depolarizing cotransport between taurine and sodium at the neuronal membrane, may illustrate an additional mechanism for the control of the electrical activity of neuronal cells.

Some observations relating to behind-body armour blunt trauma effects caused by ballistic impact.

Live, anesthetised pigs were used to assess behind-armour blunt trauma effects. The thoraco-abdominal body region was covered with varying thicknesses of Kevlar fabric packets. This soft body armour was applied, either in direct contact with the thoracic wall of the animals, or with different plastic foam sheets, so-called trauma packs, between the armour and the skin. The live animals were surgically evaluated, and then sacrificed. Blocks of soft soap were subjected to equal tests and the behind-armour indentations were measured. The results indicate that serious injury to the body armour-protected chest may be caused by the impact of nonpenetrating bullets and shotgun pellets. Severe pulmonary contusions and lacerations were found when the energy transferred through the body armour was estimated to be high.

Terminal behaviour of deforming bullets.

Some types of deforming bullets, including soft-point hunting ammunition and so-called shotgun slugs, were fired against blocks of soft soap and against the muscular parts of the hind legs of live, anesthetised pigs. The deformation, breakup, and retardation of the bullets within the targets were recorded by means of a multi-flash X-ray setup. The extent of the soft-tissue damage was evaluated by means of debridement performed by a highly skilled surgeon. The excised tissue was then accurately weighed. The potential to transfer energy of these bullets was, as could be anticipated, extremely high, and the injuries caused were very large. The relations between bullet velocity, construction, deformation, breakup, and the resulting injuries are discussed.

Wound ballistics of Swedish 5.56-mm assault rifle AK 5.

The recently adopted Swedish standard 5.56-mm assault rifle Ak 5, with ammunition 5.56-mm sk ptr 5 stkprj, closely similar to the Belgian weapon FNC, with corresponding ammunition SS 109, was fired against blocks of soft soap and against the muscular parts of the hind legs of live, anesthetised pigs. The behaviour and retardation of the bullets within the targets were recorded by means of a stereo, multiflash X-ray setup. The extent of the soft-tissue damage was evaluated in sections by means of debridement performed by a highly skilled surgeon. The excised tissue was then accurately weighed. The energy transfer characteristics and the wounding potential of the weapon are discussed.

Is the concentration of gamma-aminobutyric acid in the nerve terminal regulated via product inhibition of glutamic acid decarboxylase?

Fractions of synaptosomes were used to study the regulation of gamma-aminobutyric acid (GABA) synthesis. The isolated synaptosomes were superfused in media of various compositions. [3H]GABA and GABA released into the medium or remaining in the synaptosomes were analyzed by liquid scintillation and HPLC techniques. Different conditions, designed to increase the GABA efflux rate were used: the rate of superfusion was varied and the concentrations of K+ and Ca2+ were altered. Stimulation of GABA efflux was paralleled with an increased synthesis of GABA, since, in spite of the increased GABA efflux, a relatively constant intraterminal level was found. The findings suggest that the intraterminal concentration of GABA and thus also its synthesis is regulated via product inhibition. In addition, [3H]GABA, exogenous, and GABA, endogenous, responded to external stimulae (Ca2+, veretradine, various GABA concentrations and the glutaminase inhibitor diazo-nor-leucine) in a way which was compatible with them being localized in and/or released from different compartments.

Some observations made by intracellular recordings in a primary astrocyte culture and a glioblastoma '138 MG'.

Intracellular recordings were performed in a primary astrocyte culture from rat brain and in a human glioblastoma cell line, 138 MG. The technique proved insufficient to verify the heterogeneous composition of the primary astrocyte culture, since this study shows most cells present in the culture to have similar resting membrane potential, membrane impedance, membrane potential/impedance relationship and K+-sensitivity. With the exception of macrophages, identified by their response to externally applied yeast particles, the results do not allow the identification of different cells that are known to be present. The membrane potential of the primary astrocyte was -68 +/- 14 mV, and the membrane potential of the 138 MG cells -37 +/- 15 mV. The membrane potential of cells in the primary culture have a K+-sensitivity resembling that of astrocytes in situ, whereas the K+-sensitivity of 138 MG more resemble that of a dedifferentiated cell. A reduction of 0.95 mm Ca2+ to o mM depolarizes the astrocyte with 9.6 mV and hyperpolarizes the glioma cell 2.6 mV.