Complexos enzimáticos sobre a energia metabolizável e digestibilidade dos nutrientes do milheto para frangos de corte / Enzyme complexes on metabolizable energy and nutrient digestibility of millet for broiler chickens

Objetivou-se avaliar o efeito de complexos enzimáticos sobre a energia metabolizável e o coeficiente de digestibilidade de nutrientes do milheto para frangos de corte. Quinhentos e setenta e seis frangos machos foram distribuídos em 36 gaiolas, com três tratamentos: T1 - composição de milheto sem complexo enzimático; T2 - composição de milheto com complexo enzimático (CES) e T3 - composição de milheto com complexo enzimático (CEV). Os tratamentos foram definidos com base em seis dietas (três dietas referências e três dietas testes). As dietas testes foram obtidas pela substituição de 40% da dieta referência por milheto inteiro, e a adição de enzimas consistiu de dois complexos enzimáticos: CES, constituído pelas enzimas fitase, protease, xilanase, ß-glucanase, celulase, amilase e pectinase; e CEV. constituído pelas enzimas protease, celulase e amilase. Na fase de 11 a 20 dias, a suplementação com o CEV reduziu os valores de EMA, EMAn e CDPB. A suplementação com CES melhorou o CDPB, e não houve efeito significativo para CDMS e CDEB. Na fase de 21 a 30 dias, houve menor aproveitamento da energia e dos nutrientes com as suplementações CES e CEV. Na fase de 31 a 40 dias, as suplementações reduziram os valores de EMA, EMAn, e o complexo CEV foi efetivo em aumentar o valor de CDPB. A inclusão dos complexos enzimáticos CES (fitase, protease, xilanase, ß-glucanase, celulase, amilase e pectinase) e CEV (protease, celulase e amilase) não favoreceu a utilização da energia do milheto, no entanto melhorou o coeficiente de digestibilidade da proteína do milheto nos períodos de 11 a 20 e de 31 a 40 dias de idade.(AU)

The objective of this study was to evaluate the effect of enzymatic complexes on metabolizable energy and nutrient digestibility coefficient of millet for broilers chickens. 576 male chickens, were distributed in 36 cages with three treatments: T1 - millet composition without enzymatic complex; T2 - millet composition with enzymatic complex (ECS); and T3 - millet composition with enzymatic complex (ECV). The treatments were defined from six diets (3 reference diets and 3 test diets). The test diets were obtained from the substitution of 40% for reference diet by whole millet, and the enzyme addition consisted of two enzymatic complex, ECS constituted by phytase, protease, xylanase, ß-glucanase, cellulase, amylase and pectinase enzymes, and ECV constituted by protease, cellulase and amylase enzymes. In the 11 to 20 days phase, a supplementation with the ECV reduced the AME, AMEn and CDPB values, a ECS supplementation improved the CDPB, and there was no significant effect for CDMS and CDEB. In the 21 to 30 days phase, there were less profit of the energy and nutrients with ECS and ECV supplements. In the 31 to 40 days phase as supplements reduced the values of AME, AMEn, and the ECV complex was effective in increasing the value of CDPB. The inclusion of ECS enzymatic complexes, (phytase, protease, xylanase, ß-glucanase, cellulase, amylase and pectinase) and ECV (protease, cellulase and amylase), did not favor millet's energy utilization, however, favored the millet's protein digestibility coefficient on 11 to 20 and 31 to 40 periods.(AU)

Monitoring of urban and rural basins: water quality of Mourão basin.

The Mourão River basin is located on the central western region of the Paraná State - Brazil, between coordinates 23º 44' - 24º 25 South latitude and 52º 12' - 52º 30' West longitude, between 270 and 820 m above sea level, and 1,648.21 km2 drainage area. Water quality was evaluated by monitoring physical, chemical and microbiological parameters. Monthly samplings were performed for a year at five sites in the basin for analysis of: pH, temperature, dissolved oxygen, biochemical oxygen demand, total nitrogen, ammoniacal nitrogen, nitrite, nitrate, total phosphorus, turbidity, total solids, volatile solids and fecal coliforms. The results of the evaluated parameters showed higher values than the limits set by CONAMA Resolution 357 from 2005 for Class 2 in some samples. The Water Quality Index (WQI) indicated that 72% of samples had average quality and 28% had good quality for the Mourão River basin. Higher values of WQI were observed after rainfall period with median of 75 compared to the dry period with median of 62. The source of the Mourão River is contaminated with fecal coliforms, evidencing the real need to treat sewage in rural areas.

Influence of urban area on the water quality of the Campo River basin, Paraná State, Brazil.

The Campo River basin is located on the third plateau of the Paraná State or trap plateau of Paraná, at the middle portion between the rivers Ivaí and Piquiri, southern Brazil, between the coordinates 23° 53 and 24° 10' South Latitude and 52° 15' and 52° 31' West Longitude. The basin has 384 Km² area, being 247 km² in the municipality of Campo Mourão and 137 km² in the municipality of Peabiru, in Paraná State. The Campo River is a left bank tributary of the Mourão River, which flows into the Ivaí River. The objective of this study was to monitor water quality in the Km 119 River and the Campo River, tributaries of the Mourão River, with monthly collection of water samples to determine pH, temperature, turbidity, biochemical oxygen demand, dissolved oxygen, fecal coliforms, total solids, total nitrogen, ammoniacal nitrogen, nitrite, nitrate and total phosphorus. The results obtained were compared with the indices established by the environmental legislation and applied in the determination of the Water Quality Index (WQI) used by the Water Institute of Paraná State, regulating environmental agency. Poor water quality in these rivers presents a worrying scenario for the region, since this river is the main source of water supply for the public system. Results of organic matter, fecal coliforms and total phosphorus were higher than the limits established by Resolution CONAMA 357/2005 to river class 2, specially at downstream of the Km 119 River and the Campo River, due to the significant influence of the urban anthropic activity by the lack of tertiary treatment and also rural by the lack of basic sanitation in this area. Results of WQI of Km 119 River and do Campo River indicated that water quality can be classified as average in 71% and good in 29% of the sites evaluated.

Monitoring of urban and rural basins: water quality of Mourão basin / Monitoramento de bacias urbana e rural: análise integrada de qualidade de água da bacia hidrográfica do Rio Mourão

The Mourão River basin is located on the central western region of the Paraná State – Brazil, between coordinates 23º 44’ - 24º 25 South latitude and 52º 12’ - 52º 30’ West longitude, between 270 and 820 m above sea level, and 1,648.21 km2 drainage area. Water quality was evaluated by monitoring physical, chemical and microbiological parameters. Monthly samplings were performed for a year at five sites in the basin for analysis of: pH, temperature, dissolved oxygen, biochemical oxygen demand, total nitrogen, ammoniacal nitrogen, nitrite, nitrate, total phosphorus, turbidity, total solids, volatile solids and fecal coliforms. The results of the evaluated parameters showed higher values than the limits set by CONAMA Resolution 357 from 2005 for Class 2 in some samples. The Water Quality Index (WQI) indicated that 72% of samples had average quality and 28% had good quality for the Mourão River basin. Higher values of WQI were observed after rainfall period with median of 75 compared to the dry period with median of 62. The source of the Mourão River is contaminated with fecal coliforms, evidencing the real need to treat sewage in rural areas.

Resumo A Bacia Hidrográfica do Rio Mourão, localizada na porção centro ocidental do Estado do Paraná – Brasil, entre as coordenadas 23º 44’ - 24º 25’ Latitude Sul e 52º 12’ - 52º 30’ Longitude Oeste, entre 270 e 820 m acima do nível mar, possui área de drenagem de 1.648,21 km2. Nesta bacia foi avaliada a qualidade da água por meio de monitoramento de parâmetros físico-químicos e microbiológicos. Foram realizadas coletas mensais durante um ano em 5 pontos selecionados da bacia, sendo realizadas análises de: potencial Hidrogeniônico, temperatura, oxigênio dissolvido, demanda bioquímica de oxigênio, nitrogênio total, nitrogênio amoniacal, nitrito, nitrato, fósforo total, turbidez, sólidos totais, sólidos voláteis e coliformes fecais. Os resultados dos parâmetros avaliados indicaram valores superiores aos limites preconizados na Resolução CONAMA 357 de 2005 para rios de classe 2 em algumas amostras. Com a análise do Índice de Qualidade das Águas (IQA), obteve-se 72% das amostras com qualidade média e 28% com qualidade boa para a bacia do Rio Mourão. Os maiores valores do IQA foram observados na época de chuvas com mediana de 75 comparados ao período de seca com mediana de 62. Constatou-se que a nascente do rio Mourão está contaminada com coliformes fecais, mostrando a real necessidade de se tratar os esgotos sanitários no meio rural.

Influence of urban area on the water quality of the Campo River basin, Paraná State, Brazil / Influência da área urbana na qualidade das águas na Bacia do Rio do Campo River, estado do Paraná, Brasil

The Campo River basin is located on the third plateau of the Paraná State or trap plateau of Paraná, at the middle portion between the rivers Ivaí and Piquiri, southern Brazil, between the coordinates 23° 53 and 24° 10’ South Latitude and 52° 15’ and 52° 31’ West Longitude. The basin has 384 Km² area, being 247 km2 in the municipality of Campo Mourão and 137 km2 in the municipality of Peabiru, in Paraná State. The Campo River is a left bank tributary of the Mourão River, which flows into the Ivaí River. The objective of this study was to monitor water quality in the Km 119 River and the Campo River, tributaries of the Mourão River, with monthly collection of water samples to determine pH, temperature, turbidity, biochemical oxygen demand, dissolved oxygen, fecal coliforms, total solids, total nitrogen, ammoniacal nitrogen, nitrite, nitrate and total phosphorus. The results obtained were compared with the indices established by the environmental legislation and applied in the determination of the Water Quality Index (WQI) used by the Water Institute of Paraná State, regulating environmental agency. Poor water quality in these rivers presents a worrying scenario for the region, since this river is the main source of water supply for the public system. Results of organic matter, fecal coliforms and total phosphorus were higher than the limits established by Resolution CONAMA 357/2005 to river class 2, specially at downstream of the Km 119 River and the Campo River, due to the significant influence of the urban anthropic activity by the lack of tertiary treatment and also rural by the lack of basic sanitation in this area. Results of WQI of Km 119 River and do Campo River indicated that water quality can be classified as average in 71% and good in 29% of the sites evaluated.

Resumo A Bacia Hidrográfica Rio do Campo está situada no terceiro planalto paranaense, na porção média entre os rios Ivaí e Piquiri, Sul do Brasil, entre as coordenadas 23° 53’ e 24°10’ de Latitude Sul e 52°15’ e 52°31’ de Longitude Oeste. A bacia ocupa área de 384 Km2, sendo 247 km2 no município de Campo Mourão e 137 km2 no município de Peabiru, no estado do Paraná. O Rio do Campo é um afluente da margem esquerda do Rio Mourão, que deságua no Rio Ivaí. O objetivo deste trabalho foi monitorar a qualidade da água do rio Km 119 e rio do Campo, afluentes do Rio Mourão, com coletas mensais de amostras de água para determinação do pH, temperatura, turbidez, demanda bioquímica de oxigênio, oxigênio dissolvido, coliformes fecais, sólidos totais, nitrogênio total, nitrogênio amoniacal, nitrito, nitrato e fósforo total. Os resultados obtidos foram comparados aos limites estabelecidos na legislação ambiental vigente para poluentes na água e aplicados na determinação do Índice de Qualidade das Águas (IQA) usado pelo Instituto das Águas do Paraná, órgão ambiental fiscalizador. A má qualidade observada nestes rios é preocupante para a região que usufruiu destas águas como principal fonte de abastecimento público. Resultados de matéria orgânica, coliformes totais e fósforo total extrapolaram os padrões estabelecidos na Resolução CONAMA 357/2005 para rio classe 2, especialmente à jusante do rio Km 119 e do Rio do Campo, devido à influência significativa da atividade antrópica urbana pela falta de tratamento terciário e rural pela falta de saneamento básico nesta área. Os resultados do IQA do Rio Km 119 e Rio do Campo indicam que as águas podem ser classificadas com qualidade média em 71% e boa em 29% nos pontos avaliados.

Influence of urban area on the water quality of the Campo River basin, Paraná State, Brazil

The Campo River basin is located on the third plateau of the Paraná State or trap plateau of Paraná, at the middle portion between the rivers Ivaí and Piquiri, southern Brazil, between the coordinates 23° 53 and 24° 10 South Latitude and 52° 15 and 52° 31 West Longitude. The basin has 384 Km² area, being 247 km2 in the municipality of Campo Mourão and 137 km2 in the municipality of Peabiru, in Paraná State. The Campo River is a left bank tributary of the Mourão River, which flows into the Ivaí River. The objective of this study was to monitor water quality in the Km 119 River and the Campo River, tributaries of the Mourão River, with monthly collection of water samples to determine pH, temperature, turbidity, biochemical oxygen demand, dissolved oxygen, fecal coliforms, total solids, total nitrogen, ammoniacal nitrogen, nitrite, nitrate and total phosphorus. The results obtained were compared with the indices established by the environmental legislation and applied in the determination of the Water Quality Index (WQI) used by the Water Institute of Paraná State, regulating environmental agency. Poor water quality in these rivers presents a worrying scenario for the region, since this river is the main source of water supply for the public system. Results of organic matter, fecal coliforms and total phosphorus were higher than the limits established by Resolution CONAMA 357/2005 to river class 2, specially at downstream of the Km 119 River and the Campo River, due to the significant influence of the urban anthropic activity by the lack of tertiary treatment and also rural by the lack of basic sanitation in this area. Results of WQI of Km 119 River and do Campo River indicated that water quality can be classified as average in 71% and good in 29% of the sites evaluated.

Resumo A Bacia Hidrográfica Rio do Campo está situada no terceiro planalto paranaense, na porção média entre os rios Ivaí e Piquiri, Sul do Brasil, entre as coordenadas 23° 53 e 24°10 de Latitude Sul e 52°15 e 52°31 de Longitude Oeste. A bacia ocupa área de 384 Km2, sendo 247 km2 no município de Campo Mourão e 137 km2 no município de Peabiru, no estado do Paraná. O Rio do Campo é um afluente da margem esquerda do Rio Mourão, que deságua no Rio Ivaí. O objetivo deste trabalho foi monitorar a qualidade da água do rio Km 119 e rio do Campo, afluentes do Rio Mourão, com coletas mensais de amostras de água para determinação do pH, temperatura, turbidez, demanda bioquímica de oxigênio, oxigênio dissolvido, coliformes fecais, sólidos totais, nitrogênio total, nitrogênio amoniacal, nitrito, nitrato e fósforo total. Os resultados obtidos foram comparados aos limites estabelecidos na legislação ambiental vigente para poluentes na água e aplicados na determinação do Índice de Qualidade das Águas (IQA) usado pelo Instituto das Águas do Paraná, órgão ambiental fiscalizador. A má qualidade observada nestes rios é preocupante para a região que usufruiu destas águas como principal fonte de abastecimento público. Resultados de matéria orgânica, coliformes totais e fósforo total extrapolaram os padrões estabelecidos na Resolução CONAMA 357/2005 para rio classe 2, especialmente à jusante do rio Km 119 e do Rio do Campo, devido à influência significativa da atividade antrópica urbana pela falta de tratamento terciário e rural pela falta de saneamento básico nesta área. Os resultados do IQA do Rio Km 119 e Rio do Campo indicam que as águas podem ser classificadas com qualidade média em 71% e boa em 29% nos pontos avaliados.

Monitoring of urban and rural basins: water quality of Mourão basin

The Mourão River basin is located on the central western region of the Paraná State Brazil, between coordinates 23º 44 - 24º 25 South latitude and 52º 12 - 52º 30 West longitude, between 270 and 820 m above sea level, and 1,648.21 km2 drainage area. Water quality was evaluated by monitoring physical, chemical and microbiological parameters. Monthly samplings were performed for a year at five sites in the basin for analysis of: pH, temperature, dissolved oxygen, biochemical oxygen demand, total nitrogen, ammoniacal nitrogen, nitrite, nitrate, total phosphorus, turbidity, total solids, volatile solids and fecal coliforms. The results of the evaluated parameters showed higher values than the limits set by CONAMA Resolution 357 from 2005 for Class 2 in some samples. The Water Quality Index (WQI) indicated that 72% of samples had average quality and 28% had good quality for the Mourão River basin. Higher values of WQI were observed after rainfall period with median of 75 compared to the dry period with median of 62. The source of the Mourão River is contaminated with fecal coliforms, evidencing the real need to treat sewage in rural areas.

Resumo A Bacia Hidrográfica do Rio Mourão, localizada na porção centro ocidental do Estado do Paraná Brasil, entre as coordenadas 23º 44 - 24º 25 Latitude Sul e 52º 12 - 52º 30 Longitude Oeste, entre 270 e 820 m acima do nível mar, possui área de drenagem de 1.648,21 km2. Nesta bacia foi avaliada a qualidade da água por meio de monitoramento de parâmetros físico-químicos e microbiológicos. Foram realizadas coletas mensais durante um ano em 5 pontos selecionados da bacia, sendo realizadas análises de: potencial Hidrogeniônico, temperatura, oxigênio dissolvido, demanda bioquímica de oxigênio, nitrogênio total, nitrogênio amoniacal, nitrito, nitrato, fósforo total, turbidez, sólidos totais, sólidos voláteis e coliformes fecais. Os resultados dos parâmetros avaliados indicaram valores superiores aos limites preconizados na Resolução CONAMA 357 de 2005 para rios de classe 2 em algumas amostras. Com a análise do Índice de Qualidade das Águas (IQA), obteve-se 72% das amostras com qualidade média e 28% com qualidade boa para a bacia do Rio Mourão. Os maiores valores do IQA foram observados na época de chuvas com mediana de 75 comparados ao período de seca com mediana de 62. Constatou-se que a nascente do rio Mourão está contaminada com coliformes fecais, mostrando a real necessidade de se tratar os esgotos sanitários no meio rural.

Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa.

BACKGROUND: Heat-shock protein A2 (HspA2) is correlated with sperm maturity, function and fertility, and a dysfunctional expression of such a gene results in abnormal spermatogenesis. The purpose of this study was to compare HspA2 gene expression in spermatozoa from oligozoospermic men and normozoospermic controls. METHODS: Semen was obtained and analysed according to World Health Organization (World Health Organization, 1999) guidelines, morphology by Kruger's strict criteria. Seventeen patients with oligozoospermia and 21 fertile controls were studied. Total RNA was extracted from ejaculated and Percoll density-gradient-separated spermatozoa followed by semiquantitative RT-PCR analysis. The relative expression level of HspA2 was analysed according to the expression level of the housekeeping beta-actin gene. Serum hormonal profiles (FSH, LH and testosterone) and a peripheral karyotype were also performed. RESULTS: All patients possessed normal karyotype, and no significant hormonal differences were found between the two groups. The study group had significantly lower sperm concentration and normal morphology than the controls. Semiquantitative RT-PCR analysis of HspA2 showed significantly lower expression levels in the oligoteratozoospermic men when compared to controls (P=0.0021). CONCLUSIONS: The HspA2 gene was down-regulated in sperm from infertile men with idiopathic oligoteratozoospermia, suggesting that such anomalies of gene expression might be associated with pathogenesis in some subtypes of male infertility.

Pre-parturition profile of steroids and prostaglandin in cows with or without foetal membrane retention.

Retained foetal membranes in cattle is one of the most common complications associated to the reduction in milk yield and impaired fertility in dairy cattle. In order to determine some endocrine mechanisms controlling parturition and delivery of foetal membranes, plasma concentrations of steroids and prostanoids were determined in 20 healthy Holstein cows. Samples were taken within the interval of 5 days pre-parturition to 12h after calving. Progesterone (P4) levels were similar in cows with (PR) and without (NPR) placental retention. While the estradiol-17beta (E2) peak at parturition was lower in PR than in NPR cows, cortisol levels were greater in PR cows 12 and 24h pre-parturition. The Prostaglandin F2alpha metabolite (PGFM) levels were higher at parturition in NPR compared with the PR group, but 12h later, these levels in the PR group increased so that concentrations were greater as compared with NPR cows. The Prostaglandin E2 metabolite (PGEM), 24, 48 and 72 h pre-parturition, were higher in PR cows. However, the PGFM:PGEM ratio was greater in cows up NPR at all time when included, indicating the importance of higher levels of Prostaglandin F2alpha (PGF2alpha) than Prostaglandin E2 (PGE2) for normal placental delivery. In conclusion, placental retention was related to both estrogen and PGF2alpha deficiency, which may be a consequence of metabolic stress leading to PGE2 and maternal cortisol synthesis before parturition.

Reversed-phase liquid chromatographic method for estrogen determination in equine biological samples.

Equine unsaturated estrogens are the main components of brand formulations indicated for hormonal replacement therapy in both hypogonadic and postmenopausal women. These hormones are produced by the fetoplacental unit during equine gestation. A method is described for the quantitative determination of equilenin (EL), equilin (EQ), 17alpha-dihydroequilin (17dEQ), and estrone (El) in the plasma of a pregnant mare. Blood samples are obtained weekly during pregnancy by jugular venipuncture using sodium ethylenediaminetetracetic as the anticoagulant. For the quantitation of these estrogens, plasma is submitted to enzymatic hydrolysis followed by liquid-liquid extraction. A high-performance liquid chromatographic system equipped with a UV detector set at 220 nm and an ODS Hypersil column is used. The method met precision, specificity, and accuracy requirements. The hormonal levels determined in one target mare throughout pregnancy were 97.91 to 449.13, 116.47 to 266.02, 74.92 to 235.54, and 84.26 to 300.03 ng/mL, reaching a maximum towards the 25th, 20th, 33rd, and 27th weeks, respectively, for E1, EL, EQ, and 17dEQ. The method was successfully tested by quantitating these estrogens in the plasma from a pregnant mare. Its applicability to the study of estrogen bioavailability and bioequivalence is suggested.

[Multiple antibiotic resistance mediated by R plasmid in Shigella flexneri strains isolated in the northeast of Brazil]. / Multirresistência a antimicrobianos mediada por plasmídios R em cepas de Shigella flexneri isoladas no nordeste do Brasil.

In Shigella strains were studied the molecular mechanism that mediated the multiply antibiotic-resistance. Twenty-six strains of Shigella flexneri were utilised in this investigation. These strains were submitted to disk diffusion test, mating experiments and plasmid isolation. In relation to antibiotics resistance standard it was observed that all Shigella flexneri strains were resistant to at least, three antibiotics tested. From twenty-six Shigella flexneri strains donors submitted to conjugation process, 34.6% (nine strains) resulted in variable frequency of transconjugants. From strains that conjugated, 100%, transferred the resistance factor acquainted with ampicillin. Being that, in all transconjugants which were observed, just one plasmid with 23.1 Kb was evidenced. This plasmid found in all strains was characterised as the cause of resistance to ampicillin.

Multirresistência a antimicrobianos mediada por plasmídios R em cepas de Shigella flexneri isoladas no nordeste do Brasil / Multiple antibiotic resistance mediated by R plasmid in Shigella flexneri strains isolated in the northeast of Brazil

Em amostras de S. flexneri, isoladas no período de 1989 a 1993, foi estudado o mecanismo molecular que mediava a multirresistência. Foram utilizadas no estudo 26 amostras de S. flexneri. Estas amostras foram submetidas a teste de sensibilidade a antimicrobianos, experimentos de conjugação e extração de plasmídios. Com relação ao padrão de resistência a antimicrobianos, observou-se que todas as amostras de S. flexneri eram resistentes a pelo menos três antimicrobianos. Das 26 amostras de S. flexneri doadoras submetidas ao processo de conjugação, 34,6% (9 amostras) resultaram em uma freqüência variável de transconjugantes. Das amostras que conjugaram, 100% transferiram o fator de resistência relacionado à ampicilina; sendo que em todas as transconjugantes foi evidenciado apenas um plasmídio de 23,1Kb. Este plasmídio, encontrado em todas as amostras de Shigellas, caracterizou-se como o portador de marca de resistência para ampicilina.

In Shigella strains were studied the molecular mechanism that mediated the multiply antibiotic-resistance. Twenty-six strains of Shigella flexneri were utilised in this investigation. These strains were submitted to disk diffusion test, mating experiments and plasmid isolation. In relation to antibiotics resistance standard it was observed that all Shigella flexneri strains were resistant to at least, three antibiotics tested. From twenty-six Shigella flexneri strains donors submitted to conjugation process, 34.6% (nine strains) resulted in variable frequency of transconjugants. From strains that conjugated, 100%, transferred the resistance factor acquainted with ampicillin. Being that, in all transconjugants which were observed, just one plasmid with 23.1 Kb was evidenced. This plasmid found in all strains was characterised as the cause of resistance to ampicillin.

A field evaluation of anthelmintics for control of cyathostomes of horses in Brazil.

The anthelmintic efficacy of ivermectin (IVM), piperazine citrate (PPZ) and fenbendazole (FBZ) alone, and combinations of FBZ with varying dosages of PPZ, was evaluated for the control of strongyles of Mangalarga equids. In all cases of positive strongly egg counts per gram of faeces (EPG) before and after treatment, pure populations of cyathostome larvae with eight gut cells were consistently observed in faecal cultures. The faecal egg count reductions (FECR) were evaluated between Day 7 and Day 56 post-treatment. Significant differences (P less than or equal to 0.05) were found between Day 7 and Day 49 when comparing the mean EPG values of IVM with those of the other anthelmintics. PPZ alone and the combination of FBZ (7.5 mg kg-1) and PPZ (55 mg base kg-1) did not show significant differences during the trial; both groups exhibiting an FECR of greater than 90% between Day 7 and Day 30. Horses treated with the combinations of FBZ (7.5 mg kg-1) and PPZ at either 40 or 25 mg base kg-1, and with FBZ alone at either 7.5 or 15 mg kg-1, showed an FECR of less than 90% 2 weeks after treatment. The emergence of a degree of resistance of strongyles to FBZ and PPZ in a segment of the cyathostomes in this population is suggested.

Estudo comparativo da eficiência de Ivermectina, de Fenbendazole, de Mebendazole e de Mebendazole associado ao Citrato de Piperazina no controle de ciatostomíneos de eqüinos da raça Mangalarga Paulista / Efficacy of Ivermectin, Fenbendazole, Mebendazole and Mebendazole combined with piperazine citrate, in the control of cyathostomes of Mangalarga Paulista equids

Através de estudo comparativo, objetivou-se avaliar a eficiência anti-helmíntica de Ivermectina, de Fenbendazole, de Mebendazole e de Mebendazole associado ao Citrato de Piperazina no controle de ciatostomíneos de eqüinos da raça Mangalarga Paulista. As coproculturas realizadas antes e após os tratamentos levaram consistentemente ao encontro de populaçöes puras de ciatostomíneos com oito células intestinais. Os percentuais de eficácia foram avaliados do 7§ ao 72§ dias do pós-tratamento