Efeitos do extrato aquoso da hyptis pectinata sobre a regeneraçäo hepática após hepatectomia parcial de 70 por cento: resultados preliminares / Effects of the aqueous extract of hyptis pectinata on liver regeneration after 70 percent partial hepatectomy: preliminary results

O uso de plantas medicinais tem aumentado bastante na populaçäo mundial. O objetivo desta pesquisa é avaliar os efeitos do extrato aquoso das folhas da Hyptis pectinata, popularmente conhecida como "sambacaitá" ou "canudinho", sobre a regeneraçäo hepática após hepatectomia parcial de 70 por cento. Foram utilizados 24 ratos, divididos em 4 grupos: grupo OS, em que se realizou operaçäo simulada e aplicaçäo oral de água destilada por 4 dias; grupo OSD200, também submetido à laparotomia com manipulaçäo do fígado e aplicaçäo de 200 mg de extrato/Kg de animal durante o mesmo período; grupo HP, hepatectomizado a 70 por cento após 4 dias de aplicaçäo por via oral de água destilada; e grupo HPD200, hepatectomizado a 70 por cento após 4 dias de administraçäo de 200 mg extrato/Kg de animal. Foram dosadas fosfatase alcalina, bilirrubina total e as aminotransferases e estudou-se o estado III da funçäo mitocondrial. O grupo OSD200, quando comparado ao OS, apresentou reduçäo significativa da fosfatase alcalina. O grupo HPD200, em comparaçäo ao HP, teve reduçäo estatisticamente significativa no nível da AST e do estado III da funçäo mitocondrial.

Effect of the intake of an exclusive sucrose diet on acetaminophen hepatotoxicity in rats.

1. To test the effect of a sucrose diet on acetaminophen hepatotoxicity, 20 male Wistar rats were fasted and 20 were maintained exclusively on a sucrose diet ad libitum for 66 h. After 42 h, acetaminophen (1 g/kg) was administered through an orogastric tube to 10 fasted animals and to 10 animals on the sucrose diet, or intraperitoneally to the remaining animals. Animals were killed 24 h later and blood was collected for the measurement of alanine and aspartate aminotransferase activity and the liver was removed for macroscopic and microscopic examination. 2. Plasma alanine and aspartate aminotransferase levels were significantly lower (P less than 0.001) in all animals maintained on the sucrose diet. Macroscopic examination revealed parenchymatous necrosis in 90% (18/20) of the fasted animals but in none of the animals fed sucrose. Microscopic examination confirmed the macroscopic findings and permitted the detection of liver necrosis in one additional fasted animal. 3. We conclude that ingestion of a sucrose diet protected the animals from the hepatotoxic effects of acetaminophen regardless of the route of administration of the drug.

Effect of the intake of an exclusive sucrose diet on acetaminophen hepatotoxicity in rats

To test the effect of a sucrose diet on acetaminophen hepatotoxicity, 20 male Wistar rats were fasted and 20 were maintained exclusively on a sucrose diet ad libitum for 66 h. After 42 h, acetaminophen (1 g/kg) was administered through an orogastric tube to 10 fasted animals and to 10 animals on the sucrose diet, or intraperitoneally to the remaining animals. Animals were killed 24 h later and blood was collected for the measurement of alanine and aspartate aminotransferase activity and the liver was removel for macroscopic and microscopic examination. Plasma alanine and aspeartate aminotransferase levels were significantly lower (P<0.001) in all animals maintained on the sucrose diet. Macroscopic examination revealed parenchymatous necrosis in 90% (18/20) of the fasted animals but in none of the animals fed sucrosel. Microscopic examination confirmed the macroscopic findings and permited the detection of liver necrosis in one additional fasted animal. We conclude that ingestion of a sucrose diet protected the animals from the hepatotoxic effects of acetaminophen regardless of the route of administration of the drug