RESUMO
One nucleotide substitution in codon 130 of HLA-DQB1*03:03:02:01 results in a novel allele HLA-DQB1*03:96.
Assuntos
Alelos , Códon , Éxons , Cadeias beta de HLA-DQ , Teste de Histocompatibilidade , Humanos , Cadeias beta de HLA-DQ/genética , Taiwan , Sequência de Bases , Povo Asiático/genética , Análise de Sequência de DNA/métodos , Polimorfismo de Nucleotídeo ÚnicoRESUMO
A nucleotide mutation in codon 171 of HLA-A*11:01:01:01 results in a novel allele HLA-A*11:127N.
Assuntos
Alelos , Códon , Éxons , Antígeno HLA-A11 , Teste de Histocompatibilidade , Humanos , Taiwan , Antígeno HLA-A11/genética , Éxons/genética , Teste de Histocompatibilidade/métodos , Sequência de Bases , Análise de Sequência de DNA/métodos , Povo Asiático/genéticaRESUMO
One nucleotide substitution in codon 81 of HLA-B*40:01:01 results in a novel allele, HLA-B*40:400.
Assuntos
Alelos , Éxons , Antígeno HLA-B40 , Haplótipos , Teste de Histocompatibilidade , Humanos , Taiwan , Antígeno HLA-B40/genética , Sequência de Bases , Códon , Análise de Sequência de DNA/métodos , Povo Asiático/genética , Polimorfismo de Nucleotídeo Único , Alinhamento de Sequência , Antígenos HLA-B/genéticaRESUMO
Two nucleotide substitutions in codon 152 of HLA-C*08:01:01:01 result in a novel allele HLA-C*08:66.
Assuntos
Éxons , Antígenos HLA-C , Teste de Histocompatibilidade , Humanos , Alelos , Sequência de Bases , Códon , Teste de Histocompatibilidade/métodos , Antígenos HLA-C/genética , Alinhamento de Sequência , Análise de Sequência de DNA/métodos , TaiwanRESUMO
A nucleotide deletion in the residue 371 of HLA-A*11:01:01:01 results in a novel allele HLA-A*11:466N.
Assuntos
Éxons , Antígeno HLA-A11 , Teste de Histocompatibilidade , Humanos , Alelos , Sequência de Bases , Códon , Antígeno HLA-A11/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Deleção de Sequência , TaiwanRESUMO
Nucleotide substitution in codon 238 of HLA-C*12:02:02:01 results in a novel allele, HLA-C*12:02:53.
Assuntos
Alelos , Sequência de Bases , Códon , Éxons , Antígenos HLA-C , Teste de Histocompatibilidade , Humanos , Substituição de Aminoácidos , Povo Asiático/genética , Antígenos HLA-C/genética , Polimorfismo de Nucleotídeo Único , Alinhamento de Sequência , Análise de Sequência de DNA , TaiwanRESUMO
One nucleotide substitution in codon 264 of HLA-C*07:02:01:01 results in a novel allele, HLA-C*07:359.
Assuntos
Antígenos HLA-C , Polimorfismo de Nucleotídeo Único , Humanos , Sequência de Bases , Antígenos HLA-C/genética , Alelos , Substituição de Aminoácidos , Éxons/genética , Análise de Sequência de DNA/métodos , Teste de Histocompatibilidade , Doadores de Tecidos , TaiwanRESUMO
Objectives: It is thought that Taiwanese indigenous people were the "first people" to populate Taiwan (Formosa) having been there for over 5000 years, preceding the Dutch colonization (from 1624 to 1662) and Spanish colonization (from 1626 to 1642). Taiwan's indigenes, represented by Austronesian language speakers, currently constitute approximately 2% of the total population in Taiwan. It is unknown whether they evolved from Taiwan's Paleolithic or Neolithic cultures, arrived during or after the Neolithic period from China or Southeast Asia or both. HLA studies on the Taiwanese indigenous population have found several intriguing genetic information showing one or two relatively frequently observed alleles and a small number of relatively less frequently observed ones. We report here a relatively frequently observed HLA-C*07:359 allele in the Taiwanese indigenous population, its linkage with HLA-B*39:01, and its probable associated HLA haplotype in two Taiwanese indigenous families. HLA-C*07:359 is a rarely observed allele in the HLA-C locus in the world populations. The objective of this study is to report the allele HLA-C*07:359 that is more frequently found in the Taiwanese population, especially in the Taiwanese indigenous people, to demonstrate that it has a close linkage with HLA-B*39:01 allele in the HLA-B locus and to show the plausible deduced HLA-A-C-B-DRB1-DQB1 haplotypes in association with HLA-C*07:359 in two families of Taiwanese indigenous unrelated individuals. Materials and Methods: The samples were peripheral whole blood, with dipotassium ethylenediaminetetraacetic acid and/or acid citrate dextrose anticoagulation additives. The sequence-based typing method was employed to confirm the low incidence of the allele of HLA-C*07:359 observed in Taiwanese. Polymerase chain reaction was carried out to amplify exons 2, 3, and 4 of the HLA-A,-B,-C,-DRB1 and-DQB1 loci with group-specific primer sets. Amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocol. Results: C*07:359 is an uncommon allele in the HLA-C locus in the world general population, according to our literature review. However, in this study, it is observed in the general Taiwanese population (frequency 0.41%), especially in the Taiwanese indigenous people at a frequency of 0.23%. In addition, we deduced two probable HLA haplotypes in association with C*07:359 in two indigenous families: A*24:02-C*07:359-B*39:01-DRB1*04:36 and A*24:02-C*07:359-B*39:01-DRB1*04:04. Conclusion: The two deduced HLA haplotypes associated with the uncommon C*07:359 allele that we report here are valuable for HLA tissue typing laboratories for reference purposes and for stem cell transplantation donor search coordinators to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries for patients bearing the uncommon HLA allele. Since C*07:359 was found mostly in the Taiwanese indigenous population, we think the allele and its haplotypes we report here are important in population and anthropological studies.
RESUMO
Nucleotide substitutions in codons -1 and 84 of HLA-B*40:01:01 result in a novel allele, HLA-B*40:01:35.
Assuntos
Povo Asiático , Genes MHC Classe I , Humanos , Alelos , Povo Asiático/genética , Antígenos HLA-B/genética , NucleotídeosRESUMO
Two nucleotide substitutions in exon 3 of HLA-A*02:07:01:01 result in the novel allele, HLA-A*02:840.
Assuntos
Povo Asiático , Antígenos HLA-A , Nucleotídeos , Humanos , Alelos , Povo Asiático/genética , Éxons/genética , Antígenos HLA-A/genética , TaiwanRESUMO
Nucleotide substitution in codon 129 of HLA-B*15:01:01:01 results in a novel allele, HLA-B*15:01:17.
Assuntos
Genes MHC Classe I , Antígenos HLA-B , Humanos , Alelos , Antígenos HLA-B/genética , Códon , Povo Asiático/genética , Taiwan , Análise de Sequência de DNA/métodos , Teste de Histocompatibilidade/métodosRESUMO
One nucleotide substitution in codon 214 of HLA-C*03:04:01:01 results in a novel allele, HLA-C*03:649.
Assuntos
Antígenos HLA-C , Humanos , Sequência de Bases , Antígenos HLA-C/genética , Alelos , Éxons/genética , Códon , Taiwan , Análise de Sequência de DNA , Teste de HistocompatibilidadeRESUMO
One nucleotide substitution in codon 131 of HLA-C*03:04:01:01 results in a novel allele, HLA-C*03:04:89.
Assuntos
Antígenos HLA-C , Humanos , Sequência de Bases , Antígenos HLA-C/genética , Alelos , Teste de Histocompatibilidade , Códon , Taiwan , Análise de Sequência de DNA/métodosRESUMO
One nucleotide substitution in codon 217 of HLA-DRB1*09:01:02:01 results in a novel allele HLA-DRB1*09:01:15.
Assuntos
Cadeias HLA-DRB1 , Humanos , Cadeias HLA-DRB1/genética , Sequência de Bases , Alelos , Éxons/genética , Códon , Análise de Sequência de DNA , Taiwan , Teste de HistocompatibilidadeRESUMO
Two nucleotide substitutions in codon 24 of HLA-A*02:01:01:01 result in a novel allele HLA-A*02:570:01.
Assuntos
Antígenos HLA-A , Humanos , Alelos , Povo Asiático/genética , Sequência de Bases , Códon , Teste de Histocompatibilidade , Antígenos HLA-A/genética , Análise de Sequência de DNA , TaiwanRESUMO
One nucleotide substitution in codon 140 of HLA-DRB1*04:05:01:01 results in a novel allele, HLA-DRB1*04:358.
Assuntos
Sequência de Bases , Humanos , Alelos , Éxons/genética , Códon , Cadeias HLA-DRB1/genética , Análise de Sequência de DNA , Taiwan , Teste de HistocompatibilidadeRESUMO
One nucleotide substitution in codon 67 of HLA-B*40:02:01:01 results in a novel allele, HLA-B*40:02:03.
Assuntos
Genes MHC Classe I , Antígenos HLA-B , Humanos , Alelos , Antígenos HLA-B/genética , Códon , Povo Asiático/genética , Taiwan , Análise de Sequência de DNA , Teste de HistocompatibilidadeRESUMO
One nucleotide substitution in codon 112 of HLA-C*14:24:01 results in a novel allele, HLA-C*14:24:02.
Assuntos
Antígenos HLA-C , Humanos , Sequência de Bases , Antígenos HLA-C/genética , Alelos , Éxons/genética , Análise de Sequência de DNA , Teste de HistocompatibilidadeRESUMO
One nucleotide substitution in codon 317 of HLA-B*57:01:01:01 results in the novel allele, HLA-B*57:164.
Assuntos
Antígenos HLA-B , Humanos , Sequência de Bases , Alelos , Teste de Histocompatibilidade , Antígenos HLA-B/genética , Taiwan , Análise de Sequência de DNARESUMO
A nucleotide mutation in codon 75 of HLA-C*04:01:01:01 results in a novel allele HLA-C*04:279N.