Biodegradable Solid Polymer Electrolytes from the Discarded Cataractous Eye Protein Isolate.

The protein extracted from the discarded eye lenses postcataract surgery, referred to as the cataractous eye protein isolate (CEPI), is employed as a polymer matrix for the construction of solid polymer electrolyte species (SPEs). SPEs are expected to be inexpensive, conductive, and mechanically stable in order to be economically and commercially viable. Environmentally, these materials should be biodegradable and nontoxic. Taking these factors into account, we investigated the possibility of using a discarded protein as a polymer matrix for SPEs. Natural compounds sorbitol and sinapic acid (SA) are used as the plasticizer and cross-linker, respectively, to tune the mechanical as well as electrochemical properties. The specific material formed is demonstrated to have high ionic conductivity ranging from ∼2 × 10-2 to ∼8 × 10-2 S cm-1. Without the addition of any salt, the ionic conductivity of sorbitol-plasticized non-cross-linked CEPI is ∼7.5 × 10-2 S cm-1. Upon the addition of NaCl, the conductivity is enhanced to ∼8 × 10-1 S cm-1. This study shows the possibility of utilizing a discarded protein CEPI as an alternative polymer matrix with further potential for the construction of tunable, flexible, recyclable, biocompatible, and biodegradable SPEs for flexible green electronics and biological devices.

Gelatin-decorated Graphene oxide: A nanocarrier for delivering pH-responsive drug for improving therapeutic efficacy against atherosclerotic plaque.

The progressive inflammatory disease atherosclerosis promotes myocardial infarction, stroke, and heart attack. Anti-inflammatory drugs treat severe atherosclerosis. They are inadequate bioavailability and cause adverse effects at higher doses. A new nanomaterial coupled pH-apperceptive drug delivery system for atherosclerotic plaque is outlined here. We have synthesized a Graphene Oxide-Gelatin-Atorvastatin (GO-Gel-ATR) nanodrug characterized by spectroscopic and imaging techniques. The encapsulation efficiency of GO-Gel-ATR (79.2%) in the loading process is observed to be better than GO-ATR (66.8%). The internal milieu of the plaque cells has a pH of 6.8. The GO-Gel-ATR displays sustained and cumulative release profile at pH 6.8 compared to ATR and GO-ATR. Our proposed nanocomposite demonstrated high cytocompatibility up to 100µg/mL in foam cells induced by Oxidized-Low Density Lipoprotein (Ox-LDL) and Lipopolysaccharides (LPS) compared to normal macrophages for 24 and 48 h. The uptake efficacy of the nanodrugs is shown to be enhanced in foam cells compared to normal macrophage. Oil red O staining of foam cells with and without drugs confirmed therapeutic efficacy. Foam cells treated with nanocomposite had more lipids efflux than ATR. The finding of the in-vitro study reveals that the GO-Gel-ATR nanocomposite carriers have the potential to deliver anti-atherosclerotic drugs effectively and inhibit atherosclerotic plaque progression.

Probing silver nanoparticle mediated mitigation of UV-photolysis in proteins by electrical impedance analysis.

The dynamic equilibrium between an array of molecular forces precisely organizes the native structure of the protein. The charge on the protein, an interconnected network continuum, is crucial in determining its secondary and tertiary structure. The photolysis of the protein by ultraviolet (UV) light occurs by generating reactive oxygen intermediates from the interaction of matter and light. Herein, we have investigated the photolysis of the protein and its prevention by the pre-treatment with silver nanoparticle (AgNP) using non-faradaic electrical impedance spectroscopy (Nf-EIS). Five microliters of protein solution are used to measure its impedimetric parameters via Nf-EIS. The photoionization process sparks off an altered surface charge continuum of the protein molecules in tandem with the genesis of solvated electrons and protons, spurring an upward shift in conductivity. The AgNP pre-treatment has reduced the damaging effects of the UV radiation, which is reflected as lesser conductivity in contrast to the photolyzed protein solution. Raman Spectroscopy and circular dichroism tests affirm the trend of Nf-EIS results. These results show that Nf-EIS can evaluate protein structure analysis utilized in quality assurance and toxicity analysis for biologics.