RESUMO
The detection and molecular characterization of pathogenic human viruses in urban sewage have been used extensively to derive information on circulating viruses in given populations throughout the world. In this study, a similar approach was applied to provide an overview of the epidemiology of waterborne gastroenteritis viruses circulating in urban areas of Caracas, the capital city of Venezuela in South America. Dry season sampling was conducted in sewers and in a major river severely polluted with urban sewage discharges. Nested PCR was used for detection of human adenoviruses (HAds), while reverse transcription plus nested or seminested PCR was used for detection of enteroviruses (HuEVs), rotaviruses (HRVs), noroviruses (HuNoVs), and astroviruses (HAstVs). HRVs were fully characterized with genotype-specific primers for VP4 (genotype P), VP7 (genotype G), and the rotavirus nonstructural protein 4 (NSP4). HuNoVs and HAstVs were characterized by sequencing and phylogenetic analysis. The detection rates of all viruses were >or=50%, and all sampling events were positive for at least one of the pathogenic viruses studied. The predominant HRV types found were G1, P[8], P[4], and NSP4A and -B. Genogroup II of HuNoVs and HAstV type 8 were frequently detected in sewage and sewage-polluted river waters. This study reveals relevant epidemiological data on the distribution and persistence of human pathogenic viruses in sewage-polluted waters and addresses the potential health risks associated with transmission of these viruses through water-related environmental routes.
Assuntos
Gastroenterite/virologia , Rios/virologia , Esgotos/virologia , Vírus/classificação , Vírus/isolamento & purificação , Genótipo , Humanos , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/métodos , Análise de Sequência de DNA , População Urbana , Venezuela , Proteínas Virais/genéticaRESUMO
Dengue virus, a mosquito-borne flavivirus, is one of the most formidable public health threats in tropical and subtropical regions. As yet, there is no licensed vaccine to protect against the disease. A chimeric yellow fever (YF) 17D/dengue (DEN) type 1 virus was constructed by replacing the pre-membrane and envelope genes of YF 17D virus with those from DEN 1 VeMir95 virus, a Venezuelan isolate. The chimeric YF 17D/DEN 1 VeMir95 virus was regenerated from full-length infectious clones stably propagated in Escherichia coli by transfection of Vero cells with in vitro transcribed RNA. The chimeric virus proliferated efficiently in Vero cells ( approximately 6.6 log(10) plaque-forming units/ml). The chimeric virus was not neurovirulent to 3-week-old Swiss Webster mice inoculated by the intracerebral route, in contrast to the YF 17DD vaccine strain that was lethal for 90% of the mice. The YF 17D/DEN 1 virus at Passage 6 was more attenuated for rhesus monkeys than the YF 17DD commercial vaccine after intracerebral inoculation according to the standard neurovirulence test. This virus is a potential candidate to be included in a tetravalent DEN vaccine formulation. The availability of the cloned cDNA allows further structure/function studies on the viral envelope.
Assuntos
Vírus da Dengue/genética , Vírus Reordenados/genética , Vírus da Febre Amarela/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Chlorocebus aethiops , Vacinas contra Dengue , Vírus da Dengue/crescimento & desenvolvimento , Vírus da Dengue/patogenicidade , Genes Virais , Camundongos , Dados de Sequência Molecular , Vírus Reordenados/crescimento & desenvolvimento , Vírus Reordenados/patogenicidade , Recombinação Genética , Transfecção , Vacinas Atenuadas , Células Vero , Proteínas do Envelope Viral/genética , Virulência , Vírus da Febre Amarela/crescimento & desenvolvimento , Vírus da Febre Amarela/patogenicidadeRESUMO
The Ridascreen Norwalk-like virus enzyme immunoassay was compared with (RT)-PCR on 92 stool samples collected from children with sporadic acute gastroenteritis. Homogenization and pre-dilution of the whole stool sample resulted in high specificity (97.5%) and moderate sensitivity (60%). This assay may be useful to screen outbreaks for norovirus, but limited to detect the virus in sporadic cases of diarrhea.
Assuntos
Antígenos Virais/análise , Fezes/virologia , Gastroenterite/virologia , Técnicas Imunoenzimáticas , Norovirus/isolamento & purificação , Criança , Pré-Escolar , Humanos , RNA Viral/análise , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e EspecificidadeRESUMO
BACKGROUND: The importance of enteric viral infections in HIV-related diarrhea is uncertain. Human caliciviruses have emerged as a leading cause of acute diarrhea worldwide. OBJECTIVES: To evaluate the importance of calicivirus infections in HIV-related diarrhea. Study design 151 fecal samples collected from children and adults infected with HIV, with and without diarrhea, were examined. In addition, 89 fecal samples from non HIV-infected children and adults were also tested. Samples were analyzed by RT-PCR using primer sets specific to Norovirus genogroup I or genogroup II as well as primers designed to react with both Noroviruses and Sapovirus genus. RESULTS: Viruses were detected with equal frequencies in stools from HIV infected and non-infected adults (12%). However, specimens from HIV infected children were more likely than those of HIV-negative children to have caliciviruses (51% versus 24%, P<0.05). Viral infections were not significantly associated with diarrhea neither in children nor in adults, regardless of HIV status. Viruses genetically related to the common Lordsdale virus (Norovirus genogroup II) and London/92 virus (Sapovirus) clusters were detected circulating among children. CONCLUSIONS: These results suggest that caliciviruses may be an important opportunistic pathogen in children infected with HIV.
Assuntos
Infecções por Caliciviridae/complicações , Caliciviridae/isolamento & purificação , Diarreia/virologia , Infecções por HIV/complicações , Infecções Oportunistas Relacionadas com a AIDS/virologia , Adulto , Caliciviridae/classificação , Infecções por Caliciviridae/virologia , Pré-Escolar , DNA Viral/química , Diarreia/complicações , Fezes/virologia , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Dados de Sequência Molecular , RNA Viral/análise , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , VenezuelaRESUMO
Tranfusion-transmitted virus (TTV), a single-stranded circular DNA virus that chronically infects humans and other animals, displays a high degree of genetic diversity and was originally thought to be associated with hepatitis. The prevalences of TTV infection among different populations of humans and non-human primates from Venezuela have now been evaluated, using serum samples and three different detection tests. All three tests were PCR-based, one involving a hemi-nested PCR and primers based on the N22 open-reading-frame-1 region (N22-PCR), another employing 55 cycles with primers from the more conserved untranslated region (UTR-PCR), and the other using a hemi-nested PCR with primers from the same region (HUTR-PCR). The overall prevalences of human infection appeared much higher with the HUTR-PCR (52%) than with the N22-PCR (13%) or the UTR-PCR (5%). When the products amplified by N22-PCR from 28 human isolates of TTV were sequenced, only two genotypes of the virus were detected. The non-human sera tested came from primates kept in a zoo in north-western Venezuela. TTV DNA was detected, by HUTR-PCR, in both of the chimpanzee sera tested but not in any of the sera from the 11 New-World primates or the other 12 Old-World primates that were investigated. The results, particularly those of the HUTR-PCR, indicate that TTV infection is common in Venezuela, especially in populations, such as many Amerindian groups, who live under poor sanitary conditions. Although TTV infection may be relatively rare among non-human primates from the New World, this will have to be investigated further, using many more samples collected throughout the Americas.
Assuntos
Infecções por Circoviridae/epidemiologia , Doenças dos Primatas/epidemiologia , Torque teno virus/genética , Adulto , Idoso , Animais , Infecções por Circoviridae/etnologia , DNA Viral/sangue , Feminino , Genótipo , Humanos , Indígenas Sul-Americanos , Masculino , Pessoa de Meia-Idade , Filogenia , Reação em Cadeia da Polimerase/métodos , Prevalência , Saúde da População Rural , Venezuela/epidemiologiaRESUMO
In Venezuela, the isolation of hantaviruses from rodents and the detection, in 1999, of a clinically confirmed human case of hantavirus infection led to increased interest in these viruses. In an attempt to estimate the problem posed by such viruses in Venezuela, ELISA based on purified, recombinant, nucleoprotein were used to check 1380 human serum samples for the presence of IgG antibodies to hantavirus. The ELISA results, as confirmed by indirect immunofluorescence and Western-blot assays, indicated that 23 (1.7%) of the serum samples contained antibodies to hantaviruses. Seroprevalences were similar among all age-groups and for both genders and were no higher among rural populations with a relatively high risk of exposure to rodents than among the overall study population. Although the numbers of samples involved were small, the seroprevalence among the subjects who were residents of Carabobo state was much higher than the overall value (10.3% v. 1.7%; P < 0.01). Human infection with hantavirus appears uncommon but widely distributed in Venezuela.
Assuntos
Anticorpos Antivirais/análise , Infecções por Hantavirus/imunologia , Imunoglobulina G/análise , Orthohantavírus/imunologia , Adolescente , Adulto , Western Blotting/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Imunofluorescência/métodos , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Soroepidemiológicos , Venezuela/epidemiologiaRESUMO
Hepatitis B virus (HBV) infection among Venezuelan populations of African origin was analyzed. These populations exhibited lower HBV prevalence than the one found in the African continent. Sequence analysis of 6 isolates showed that 3 belonged to genotype F, while the 3 others were HBV genotype A. HBV genotype A was more common in the Afro-Venezuelan groups than in the general Venezuelan population. This might reflect the introduction of genotype A during the slavery period. The absence of the African genotype E among these isolates supports the hypothesis of a recent origin for this HBV genotype. HBV genotype F has already been introduced to these relatively isolated communities.
Assuntos
Vírus da Hepatite B/classificação , África/etnologia , Sequência de Aminoácidos , Genótipo , Vírus da Hepatite B/genética , Humanos , Dados de Sequência Molecular , VenezuelaRESUMO
The genotypes of hepatitis B (HBV) and delta (HDV) viruses circulating among Venezuelan Amerindian populations, where these viruses are endemic, were determined by sequencing of PCR amplified products from HBsAg positive sera. HDV genotype I (n = 7, 6 from West Amerindians), and III (n = 5, 4 from South Amerindians), were found. Only one HDV genotype I isolate was associated with HBV genotype D, 4 HDV genotype I and 2 HDV genotype III infected individuals were co-infected with HBV genotype F. The failure to detect the South American HDV genotype III in West Amerindians might be related to the outbreak of fulminant hepatitis with high mortality rate occurred between 1979 and 1982, probably affecting more the Amerindians infected with HDV genotype III. These results suggest the circulation of HDV genotype I among Amerindians, probably introduced through European immigrations, and that this HDV genotype is able to replicate in association with HBV genotype F.
Assuntos
Biomarcadores/análise , DNA Viral/análise , Anticorpos Anti-Hepatite/análise , Vírus da Hepatite B/genética , Hepatite D Crônica/complicações , Hepatite D/complicações , Hepatite D/genética , Vírus Delta da Hepatite/genética , Indígenas Sul-Americanos/genética , RNA Viral/análise , Sequência de Aminoácidos , Sequência de Bases , Biomarcadores/sangue , DNA Viral/sangue , Genótipo , Anticorpos Anti-Hepatite/sangue , Anticorpos Anti-Hepatite/imunologia , Hepatite B/sangue , Hepatite B/virologia , Antígenos de Superfície da Hepatite B/análise , Antígenos de Superfície da Hepatite B/sangue , Vírus da Hepatite B/isolamento & purificação , Hepatite D/sangue , Hepatite D/epidemiologia , Hepatite D/virologia , Hepatite D Crônica/sangue , Hepatite D Crônica/epidemiologia , Hepatite D Crônica/virologia , Vírus Delta da Hepatite/isolamento & purificação , Humanos , Indígenas Sul-Americanos/classificação , Filogenia , Reação em Cadeia da Polimerase , RNA Viral/sangue , Alinhamento de Sequência , Venezuela/epidemiologiaRESUMO
Information on infection with hepatitis C virus (HCV) in South America is scarce. The seroprevalences of antibodies to HCV among urban, rural and Amerindian populations from Venezuela, and the genotypes of the HCV isolates recovered, were therefore determined. A total of 2592 sera were tested with an immuno-assay which was developed in-house and based on synthetic peptides. Each reactive sample was then re-tested, using other enzyme immuno-assays and a reverse-transcription, nested PCR, and any sample confirmed positive (in any test) was considered HCV-positive. Genotypes were determined by analysis of RFLP. Overall, 39 (1.5%) of the samples were found HCV positive. The results of the immuno-assays indicated that the seroprevalence of HCV markers among the Amerindians investigated (23/1082, or 2.1%) was significantly higher than that among the other subjects (16/1510, or 1.1%; P = 0.02). No such difference was observed in the numbers of subjects confirmed positive by PCR, however (6/1082 v. 10/1510), and some of the anti-HCV reactivity observed among Amerindians may have been the result of cross-reactivity with parasitic infections. The relative low prevalence of active HCV infection (16/2582, or 0.6%) and the HCV genotypes observed (mainly genotype 1) are in agreement with the results of previous studies indicating that HCV is not autochthonous to South America. However, it is clear that the virus may now be found even in isolated Amerindian populations. The in-house, synthetic-peptide-based immuno-assay seems to be a valuable tool for epidemiological studies.
Assuntos
Hepatite C Crônica/epidemiologia , Programas de Rastreamento/métodos , Kit de Reagentes para Diagnóstico , Adolescente , Adulto , Feminino , Genótipo , Hepacivirus/genética , Hepacivirus/isolamento & purificação , Hepatite C Crônica/imunologia , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Polimorfismo de Fragmento de Restrição , Gravidez , Prevalência , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Saúde da População Rural/estatística & dados numéricos , Sensibilidade e Especificidade , Estudos Soroepidemiológicos , Saúde da População Urbana/estatística & dados numéricos , Venezuela/epidemiologiaRESUMO
We have sequenced the genes encoding the inner capsid protein VP6 and the outer capsid glycoprotein VP7 of the subgroup (SG) I equine rotavirus strain H-1 (P9[7], G5). The VP6 and VP7 proteins of the equine rotavirus strain H-1 shared a high degree of sequence and deduced amino acid identity with SG I porcine strains and serotype G5 porcine strains, respectively. Previous sequence analyses of the genes encoding the outer capsid spike protein VP4 and the nonstructural proteins NSP1 and NSP4 of equine H-1 strain also revealed a high degree of sequence and deduced amino acid homology with the prototype porcine rotavirus strain OSU (P9[7], G5). We have also confirmed and extended the VP4 and VP7 antigenic relatedness of equine rotavirus strain H-1 to porcine strains of P9[7] and G5 serotype specificities isolated in the United States, Venezuela, Argentina, and Australia based on cross-neutralization studies. In addition, the pathogenicity of tissue culture-adapted equine H-1, H-2, FI-14, FI-23, and L338, and porcine OSU rotavirus strains was compared in the neonatal mouse model. The 50% diarrhea dose (DD50) of equine H-1 was similar to that of porcine OSU and equine H-2 and L338 strains, while the DD50 of equine H-2 was > or = 50 or 315-fold lower than those of equine FI-14 or FI-23, respectively. Our sequence comparison of NSP4 of the rotavirus strains tested potentially identified amino acid residue 136, within the variable region spanning amino acids 130 to 141, as playing a role in virulence. Taken together, there is strong support to suggest that the equine rotavirus strain H-1 may represent an example of interspecies transmission from pigs to horses.
Assuntos
Proteínas do Capsídeo , Doenças dos Cavalos/transmissão , Infecções por Rotavirus/veterinária , Rotavirus , Doenças dos Suínos/transmissão , Sequência de Aminoácidos , Animais , Antígenos Virais/imunologia , Capsídeo/química , Capsídeo/genética , Capsídeo/imunologia , Doenças dos Cavalos/virologia , Cavalos , Camundongos , Dados de Sequência Molecular , Rotavirus/genética , Rotavirus/imunologia , Rotavirus/patogenicidade , Infecções por Rotavirus/transmissão , Infecções por Rotavirus/virologia , Suínos , Doenças dos Suínos/virologiaRESUMO
OBJECTIVE: Silent infection by hepatitis B virus (HBV) occurs in the absence of serological markers for the virus. This type of occult infection is generally chronic, asymptomatic, and associated with low levels of viral replication. This study determined the presence of HBV DNA in the sera of blood donors who were negative for serological markers that were tested during screening, with the goal of evaluating the impact of silent HBV infection in posttransfusion hepatitis B in Venezuela. METHODS: A total of 2,075 sera were tested in 53 serum pools of 25-50 donations (0.5-1.0 mL from each sample). The pools were subjected to ultracentrifugation prior to DNA extraction by the proteinase K, phenol/chloroform method. RESULTS: No HBV DNA was found in any of the pools by nested polymerase chain reaction, using primers for highly conserved regions of the genes that code for the surface antigen and for the viral capsid. Aminotransferase levels were normal in 98% of 200 sera that were tested. CONCLUSIONS: These results suggest that there is a low risk of acquiring posttransfusion hepatitis B in Venezuela.
Assuntos
Doadores de Sangue , DNA Viral/análise , Vírus da Hepatite B/genética , Hepatite B/diagnóstico , Hepatite B/epidemiologia , Reação Transfusional , Adolescente , Adulto , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Fatores de Risco , Venezuela/epidemiologiaRESUMO
Acute bilateral parotitis is a common clinical feature of various infectious and autoimmune, metabolic, and drug-related conditions. We describe a unique case of bilateral inflammatory enlargement of the parotid glands in an immunocompetent patient with dengue fever. Evidence of dengue virus in the saliva is also provided for the first time.
Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/complicações , Parotidite/etiologia , Doença Aguda , Animais , Linhagem Celular , Dengue/virologia , Vírus da Dengue/genética , Humanos , Masculino , Pessoa de Meia-Idade , Parotidite/patologia , RNA Viral/sangue , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Saliva/virologiaRESUMO
Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, from children with gastroenteritis. Astrovirus type 1 was the most frequently detected virus.
Assuntos
Infecções por Astroviridae/epidemiologia , Gastroenterite/epidemiologia , Mamastrovirus/classificação , Mamastrovirus/genética , Adolescente , Adulto , Distribuição por Idade , Infecções por Astroviridae/diagnóstico , Infecções por Astroviridae/virologia , Criança , Pré-Escolar , Colômbia/epidemiologia , DNA Viral/genética , Fezes/virologia , Gastroenterite/diagnóstico , Gastroenterite/virologia , Humanos , Lactente , Recém-Nascido , Mamastrovirus/isolamento & purificação , Pessoa de Meia-Idade , Dados de Sequência Molecular , Filogenia , Análise de Sequência de DNA , Venezuela/epidemiologiaRESUMO
Forty-three stool samples from 27 human immunodeficiency virus (HIV)-seropositive children and 38 samples from 38 HIV-negative children, collected during a 15-month period, were examined for enteric viruses. Diagnostic assays included enzyme immunoassays for rotavirus, adenovirus, and Norwalk virus; polyacrylamide gel electrophoresis for picobirnavirus and atypical rotavirus; and PCR for astrovirus and enterovirus. Specimens from HIV-positive children were more likely than those of HIV-negative children to have enterovirus (56 versus 21%; P < 0.0002) and astrovirus (12 versus 0%; P < 0.02), but not rotavirus (5 versus 8%; P > 0.5). No adenoviruses, picobirnaviruses, or Norwalk viruses were found. The rates of virus-associated diarrhea were similar among HIV-positive and HIV-negative children. Enteroviruses were excreted for up to 6 months in HIV-positive children; however, no evidence for prolonged excretion of poliovirus vaccine was observed. These results suggest that although infection with enterovirus and astrovirus may be frequent in HIV-infected children, enteric viruses are not associated with the diarrhea frequently suffered by these children.
Assuntos
Diarreia/virologia , Infecções por Enterovirus/complicações , Infecções por HIV/complicações , Infecções por Vírus de RNA/complicações , Infecções por Adenovirus Humanos/complicações , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos/isolamento & purificação , Pré-Escolar , Diarreia/complicações , Enterovirus/genética , Enterovirus/isolamento & purificação , Infecções por Enterovirus/virologia , Fezes/virologia , Infecções por HIV/virologia , Humanos , Lactente , Recém-Nascido , Mamastrovirus/genética , Mamastrovirus/isolamento & purificação , Dados de Sequência Molecular , Infecções por Vírus de RNA/virologia , Vírus de RNA/genética , Vírus de RNA/isolamento & purificaçãoRESUMO
The Instituto Venezolano de Investigaciones Cientificas (IVIC) is a government-funded multidisciplinary academic institution dedicated to research, development and technology in many areas of knowledge. Biomedical projects and publications comprise about 40% of the total at IVIC. In this article, we present an overview of some selected research and development projects conducted at IVIC which we believe contain new and important aspects related to malaria, ancylostomiasis, dengue fever, leishmaniasis and tuberculosis. Other projects considered of interest in the general area of tropical medicine are briefly described. This article was prepared as a small contribution to honor and commemorate the centenary of the Instituto Oswaldo Cruz.
Assuntos
Academias e Institutos , Pesquisa , Medicina Tropical , Animais , Dengue/tratamento farmacológico , Dengue/prevenção & controle , Humanos , Malária/tratamento farmacológico , Malária/prevenção & controle , Esquistossomose/tratamento farmacológico , Esquistossomose/epidemiologia , Esquistossomose/prevenção & controle , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , Tuberculose Resistente a Múltiplos Medicamentos/prevenção & controle , Venezuela/epidemiologiaRESUMO
Previous sequence analyses of the rotavirus nonstructural NSP4 from human and some animal rotavirus strains revealed the presence of three distinct NSP4 alleles or genetic groups. To examine the species of origin relatedness and diversity of NSP4, the nucleotide and deduced amino acid sequences of the gene encoding the NSP4 from 15 animal rotavirus strains of porcine, equine, bovine, lapine and canine origin were determined and compared to human and other animal strains sequenced previously. Lapine and equine strains were shown to belong to the NSP4 genotype A. Murine NSP4 sequences formed a previously unrecognized fourth distinct NSP4 genotype (genotype D) that was more divergent compared to NSP4 genotype A, B, and C than the latter three are among each other. Within NSP4 genotypes, strains isolated from rabbits, horses, cows (genotype A) and pigs (genotype B) clustered according to species of origin, suggesting a conserved pattern of evolution within species. NSP4 sequence comparison among one wildtype and two tissue culture-adapted lapine strains, known to cause disease in neonatal rabbits, failed to identify amino acid changes within the variable region spanning amino acids 130 to 141, suggesting that disease in rabbits is the result of the lapine virus infection and replication, including production of the NSP4 enterotoxin.
Assuntos
Glicoproteínas/genética , Infecções por Rotavirus/veterinária , Rotavirus/genética , Proteínas não Estruturais Virais/genética , Sequência de Aminoácidos , Animais , Bovinos , Cães , Genes Virais , Glicoproteínas/química , Humanos , Dados de Sequência Molecular , Filogenia , Coelhos , Rotavirus/química , Rotavirus/classificação , Infecções por Rotavirus/virologia , Análise de Sequência de DNA , Especificidade da Espécie , Toxinas Biológicas , Proteínas não Estruturais Virais/químicaRESUMO
In four cities of Venezuela a study was carried out to evaluate the epidemiological, clinical, and etiological characteristics of acute diarrhea in children under 5 years of age. The study was done between June 1993 and May 1995 and involved children who were seen in a hospital, 2,552 with diarrhea and 793 controls. The Fisher exact test was used for the statistical analysis of the results. Rotaviruses were the most important agents, both in terms of their frequency (30%) and their association with dehydration (58%). Following in importance were Campylobacter spp. (13%) and Escherichia coli classical O serogroups (9%), but their association with diarrhea was only statistically significant among children less than 3 months old, a fact that is particularly important from the standpoint of treatment. The importance of age was confirmed as a determining factor in the prevalence and severity of diarrhea.
Assuntos
Diarreia/epidemiologia , Doença Aguda , Demografia , Humanos , Fatores Socioeconômicos , Venezuela/epidemiologiaRESUMO
Anti-hepatitis B core antigen (HBcAg)-positive hepatitis B surface antigen (HBsAg)-negative plasma samples from blood donors were tested by nested PCR. DNA positivity was more significantly associated with high levels of anti-HBcAg than with low levels of anti-HBsAg antibodies. Analysis of a dilution of anti-HBcAg antibodies might result in a more rational exclusion of anti-HBcAg-positive HBsAg-negative samples, reducing the number of donations discarded and enabling more countries to incorporate anti-HBcAg testing.
Assuntos
Antígenos do Núcleo do Vírus da Hepatite B/imunologia , Antígenos de Superfície da Hepatite B/imunologia , Vírus da Hepatite B/genética , Hepatite B/diagnóstico , Anticorpos Antivirais/análise , Doadores de Sangue , DNA Viral/análise , Hepatite B/imunologia , Vírus da Hepatite B/imunologia , Humanos , Reação em Cadeia da Polimerase , VenezuelaRESUMO
An enzyme immunoassay based on three synthetic peptides from the core, NS4, and NS5 regions of hepatitis C virus allowed the detection of antibodies in 100% of immunocompetent infected patients and in 91% of immunocompromised patients (hemodialysis and hemophiliac patients). Immune impairment seemed to restrict the spectrum of antibody isotypes reacting to the core peptide.
Assuntos
Anticorpos Antivirais/sangue , Hepacivirus/imunologia , Hepatite C/imunologia , Hospedeiro Imunocomprometido/imunologia , Sequência de Aminoácidos , DNA Viral/análise , Hemofilia A/imunologia , Hemofilia A/virologia , Hepacivirus/química , Humanos , Técnicas Imunoenzimáticas , Falência Renal Crônica/imunologia , Falência Renal Crônica/terapia , Falência Renal Crônica/virologia , Dados de Sequência Molecular , Diálise Renal , Proteínas não Estruturais Virais/análise , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/imunologiaRESUMO
The effect of replacement of gene 11 of rotavirus SA-11 by a gene carrying a head to tail duplication obtained from a swine rotavirus strain was studied. The swine rotavirus strain with a duplicated gene (CC86) exhibits both a phenotype that allows to overgrow other viral strains when coinfected and an increased plaque size when plated in both CV-1 and MA-104 monkey kidney cells. Using reassortment methods the duplicated gene of the swine rotavirus was introduced into the SA-11 virus, replacing the regular gene 11. The reassorted strain was characterized to find out the origin of each of the other viral gene segments. Based on electrophoretic mobilities segments 1, 2, 3, 5, 7, 8 and 10 were identified as of SA-11. The SA-11 origin of the segments 4, 6 and 9 was confirmed by neutralization with polyclonal and monoclonal antibodies and by ELISA. The results suggest that the new reassortant virus was a monoreassortant carrying SA-11 genes except the duplicated gene originated from the swine virus CC86. The ability to in vivo replicate and to synthesize viral proteins was identical in the reassorted virus and the parental strains. Sequence analysis indicates that the new phenotype does not originate in the duplication of gene 11 but possibly from mutations in the coding region of NSP5 gene that may result in different phosphorylation patterns of the protein.