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1.
Chem Biodivers ; 21(8): e202302059, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38736027

RESUMO

This study extracted and purified a polysaccharide from Rehmanniae radix praeparata (RGP) with an average molecular weight. The structural characteristics of RGP and its iron (III) complex, RGP-Fe(III), were examined for their antioxidant properties and potential in treating iron deficiency anemia (IDA). Analysis revealed that RGP comprised Man, Rha, Gal, and Xyl, with a sugar residue skeleton featuring 1→3; 1→2, 3; and 1→2, 3, 4 linkages, among others. RGP-Fe(III) had a molecular weight of 4.39×104 Da. Notably, RGP-Fe(III) exhibited superior antioxidant activity compared to RGP alone. In IDA rat models, treatment with RGP-Fe(III) led to increased weight gain, restoration of key blood parameters including hemoglobin, red blood cells, and mean hemoglobin content, elevated serum iron levels, and decreased total iron-binding capacity. Histological examination revealed no observable toxic effects of RGP-Fe(III) on the liver and spleen. These findings suggest the potential of RGP-Fe(III) as a therapeutic agent for managing IDA and highlight its promising antioxidant properties.


Assuntos
Anemia Ferropriva , Antioxidantes , Polissacarídeos , Rehmannia , Animais , Polissacarídeos/farmacologia , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Rehmannia/química , Ratos , Antioxidantes/farmacologia , Antioxidantes/química , Antioxidantes/isolamento & purificação , Anemia Ferropriva/tratamento farmacológico , Compostos Férricos/química , Masculino , Ferro/química , Baço/efeitos dos fármacos , Ratos Sprague-Dawley , Fígado/efeitos dos fármacos , Fígado/metabolismo , Modelos Animais de Doenças
2.
Zhongguo Zhong Yao Za Zhi ; 47(14): 3773-3780, 2022 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-35850834

RESUMO

In this study, the microwave-assisted method was used to synthesize seleno-polysaccharides from Lonicerae Japonicae Flos, and the optimal process conditions were optimized.Meanwhile, the immunological activity of seleno-polysaccharides from Lonicerae Japonicae Flos was investigated.The results showed that under the conditions of nitric acid concentration of 0.512%, reaction temperature of 70.0 ℃, microwave power of 600 W, reaction time of 44.0 min, and m(Na_2SeO_3)∶m(polysaccharides)=1.0, the selenium content of Lonicerae Japonicae Flos seleno-polysaccharides was 3.89 mg·g~(-1).The results of in vitro immunoassay showed that polysaccharides and seleno-polysaccharides from Lonicerae Japonicae Flos could promote the proliferation of mouse RAW264.7 macrophages as compared with the conditions in the normal group.Compared with Lonicerae Japonicae Flos polysaccharides, Lonicerae Japonicae Flos seleno-polysaccharides could significantly facilitate the proliferation of mouse RAW264.7 macrophages and promote the production of IL-1ß and TNF-α at the concentration of 20-160 µg·mL~(-1).This study provided references for broadening the application of polysaccharides from Lonicerae Japonicae Flos.


Assuntos
Medicamentos de Ervas Chinesas , Lonicera , Animais , Cromatografia Líquida de Alta Pressão , Camundongos , Micro-Ondas , Polissacarídeos/farmacologia , Controle de Qualidade
3.
Zhongguo Zhong Yao Za Zhi ; 47(11): 2938-2946, 2022 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-35718515

RESUMO

The present study explored the optimum synthesis process of Rehmannia glutinosa seleno-polysaccharides with acetic acid as a catalyst, characterized the structure of R. glutinosa seleno-polysaccharides by Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM), thermogravimetry(TG), and atomic force microscopy(AFM), and preliminarily investigated the immunological activity of R. glutinosa seleno-polysaccharides. The results showed that the optimal conditions for the synthesis of R. glutinosa seleno-polysaccharides included m(acetic acid)∶m(R. glutinosa polysaccharides)=0.80, m(Na_2SeO_3)∶m(R. glutinosa polysaccharides)=1.25, reaction temperature of 80.0 ℃, and reaction time of 7.0 h. Under these conditions, the selenium content of R. glutinosa seleno-polysaccharides was 2.239 mg·g~(-1). The acetic acid catalysis method was milder than the nitric acid method, without affecting the structure of polysaccharides. The results of IR, SEM, TG, and AFM showed that R. glutinosa seleno-polysaccharides were properly prepared. The results of immunological activity showed that compared with the control group, R. glutinosa seleno-polysaccharides could significantly promote the phagocytic capacity of mouse monocyte macrophages and improve the spleen index and thymus index of mice. In the concentration range of 15-240 µg·mL~(-1), the proliferation of spleen lymphocytes of mice was strengthened, and the IL-2 and IFN-γ secretion by Th1 cytokines was promoted. This study can provide references for the further development and application of R. glutinosa polysaccharides.


Assuntos
Rehmannia , Animais , Citocinas , Camundongos , Polissacarídeos/química , Polissacarídeos/farmacologia , Rehmannia/química , Espectroscopia de Infravermelho com Transformada de Fourier , Baço
4.
Zhongguo Zhong Yao Za Zhi ; 47(6): 1539-1545, 2022 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-35347951

RESUMO

This study aims to acetylate Rehmannia glutinosa polysaccharides by acetic anhydride method, optimize process parameters and evaluate their antioxidant activity. With the degree of substitution(D_s) as a criterion, the effects of reaction time, acetic anhydride-to-polysaccharides ratio and temperature were investigated. Process parameters were optimized by single-factor experiment and response surface methodology. The infrared spectroscopy(IR) and scanning electron microscopy(SEM) proved the successful acetylation and were employed to preliminarily analyze the structural characteristics of acetylated derivatives. The results showed that the D_s was 0.327 under the optimal technological conditions, including m(acetic anhydride):m(R. glutinosa polysaccharides)=2.70, reaction time 3.0 h and temperature 48 ℃. Further, the antioxidant properties of acetylated derivatives were investigated in vitro and acetylation was found effective to improve the antioxidant activity of R. glutinosa polysaccharides. This study provides a reference for the further development and application of R. glutinosa polysaccharides.


Assuntos
Rehmannia , Acetilação , Antioxidantes/química , Antioxidantes/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Rehmannia/química
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