RESUMO
Syphilitic proctitis is a rare sexually transmitted disease caused by spirochete pallidum infecting the rectal mucosa. It usually has no specific clinical manifestations and is easily misdiagnosed with other rectal and anal diseases such as rectal cancer, malignant lymphoma, inflammatory bowel disease, etc.. Therefore, diagnosis of the disease is difficult and treatment options are often unreasonable. A case of syphilitic proctitis in our hospital with "rectal mass" as the main manifestation is reported as follows and relevant literature is reviewed. At the same time, we studied and analyzed the clinical and histological characteristics and differential diagnosis of syphilitic proctitis to further deepen the understanding of this disease.
RESUMO
A 46-year-old male visited our hospital with blood stool and constipation. Colonoscopy revealed a broad-based protruded lesion in the rectum.The endoscopic ultrasonography showed the lesion invaded the submucosa, and the boundary between the local and intrinsic muscular layer was not clear. Transanal local excision was conducted, the pathology showed a rare case of mucosal prolapse syndrome merging chronic suppurative inflammation.
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The Hippo signaling pathway is involved in the formation and development of the cardiovascular system. In the present study, the effects of WWC family member 3 (WWC3) on vascular smooth muscle cells (VSMCs) following injury were investigated, in addition to the associated mechanisms underlying this process. Plateletderived growth factor BB (PDGFBB) was used as a cell injury factor, and rats with balloon injuries were used as a model of carotid intimal injury. Furthermore, the expression levels of WWC3 in VSMCs and arteries postinjury were investigated, in addition to the effect of WWC3 on the proliferation and migration of VSMCs. The results demonstrated that following injury, WWC3 expression was suppressed in VSMCs and the rat carotid artery, and the activity of the Hippo signaling pathway was significantly downregulated. In addition, the expression of YY1associated protein1 (YAP) and a number of its downstream target genes, including connective tissue growth factor (CTGF), were enhanced, thus enhancing the proliferation and migration of VSMCs. Knockdown of WWC3 suppressed the levels of large tumor suppressor kinase 1 (LATS1) expression and YAP phosphorylation, and the expression of YAP, CTGF and cyclin E was subsequently enhanced, thus promoting cell proliferation and migration. Similar results were obtained following overexpression of WWC3. Treatment with PDGFBB was revealed to suppress the proliferation and migration of VSMCs transfected with the WWC3 plasmid, compared with VSMCs transfected with an empty vector. The present study demonstrated that WWC3 may interact with LATS1 in order to upregulate the Hippo signaling pathway via coimmunoprecipitation and enhancement of the phosphorylation of LATS1, in addition to the corresponding suppression of the nuclear import of YAP. However, VSMCs transfected with WWC3 plasmid with a deletion of the WW domain fail to exhibit this effect. These results suggested that WWC3 expression is downregulated in VSMCs during neointimal hyperplasia following injury (PDGFBB stimulation or balloon injury). WWC3 upregulates the activity of the Hippo signaling pathway, and weakens the proliferation and migration of VSMCs. Furthermore, the results of the present study suggested that WWC3 may interact with LATS1 to promote the phosphorylation of YAP and reduce its nuclear translocation, upregulate the activity of the Hippo pathway, and suppress the proliferation and migration of VSMCs following injury.
Assuntos
Peptídeos e Proteínas de Sinalização Intracelular/genética , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais , Lesões do Sistema Vascular/etiologia , Lesões do Sistema Vascular/metabolismo , Animais , Becaplermina , Movimento Celular , Proliferação de Células , Expressão Gênica , Técnicas de Silenciamento de Genes , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Masculino , Músculo Liso Vascular/metabolismo , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/metabolismo , Ligação Proteica , Transporte Proteico , Proteínas Proto-Oncogênicas c-sis/farmacologia , RatosRESUMO
Angioleiomyoma (ALM) is a rare benign tumor, which is generally occurs on extremity, particularly the lower legs. It is composed of varied smooth muscle bundles and vascular channels. In this letter to editor, we describe the first case report of gastric ALM.
Assuntos
Angiomioma/cirurgia , Endoscopia Gastrointestinal/métodos , Neoplasias Gástricas/cirurgia , Angiomioma/diagnóstico por imagem , Angiomioma/patologia , Feminino , Humanos , Pessoa de Meia-Idade , Neoplasias Gástricas/diagnóstico por imagem , Neoplasias Gástricas/patologiaRESUMO
Gastric cancer (GC) is one of the commonest cancers with high mortality. Despite improvement in early detection and treatments, the outcome of advanced GC remains unsatisfactory due to the poor understanding of the intricate pathogenesis of GC. GC is a multifactorial and multistep disease which involves activation of oncogenes and inactivation of tumor suppressor genes. Ubiquitin proteasome system (UPS) catalyzing many critical protein substrates is involved in initiation and development of cancer. F-box proteins (FBPs) are the main functional components of UPS. Accumulated evidence strongly suggests that abnormal regulations of FBPs contribute to uncontrolled proliferation, genomic instability and cancer. In this review, we discuss how the dysregulated FBPs promote the occurrence and development of GC.
Assuntos
Proteínas F-Box/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias Gástricas/metabolismo , Proliferação de Células , Proteínas F-Box/genética , Instabilidade Genômica , Humanos , Neoplasias Gástricas/genética , UbiquitinaçãoRESUMO
OBJECTIVES: Depletion of early growth response factor-1 (Egr-1) by a DNA enzyme, ED5, inhibits neointimal hyperplasia (NH) following vascular injury by an unknown mechanism. The aim of this study was to characterize the effects of ED5 in a rat carotid injury model in order to elucidate the mechanism by which ED5 inhibits NH. METHODS: ED5 was transfected into the arterial wall of Wistar rats using FuGENE6 transfection reagent following artery balloon injury. Hematoxylin and eosin staining, immunohistochemistry, real-time reverse transcription polymerase chain reaction and Western blotting analysis were used to characterize the response to ED5. RESULTS: NH decreased significantly in the ED5- plus FuGENE6-treated rats (p < 0.05) compared with the control groups, and this was accompanied by a reduced inflammatory response. Egr-1 mRNA and protein levels were significantly decreased in the ED5-treated group, as expected. The decrease in Egr-1 was accompanied by decreases in the mRNA and protein levels of PDGF-BB, Cyclin D1, CDK4, MCP-1, and ICAM-1 (p < 0.05). CONCLUSIONS: Transfection of the Egr-1-specific synthetic DNA enzyme ED5 significantly reduced NH after injury in rats, at least in part, as a result of decreased expression of downstream proliferative genes such as PDGF-BB, Cyclin D1, CDK4, and the inflammatory factors MCP-1 and ICAM-1.
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Artérias Carótidas/patologia , Lesões das Artérias Carótidas/tratamento farmacológico , DNA de Cadeia Simples/administração & dosagem , Proteína 1 de Resposta de Crescimento Precoce/antagonistas & inibidores , Neointima/prevenção & controle , Animais , Becaplermina , Lesões das Artérias Carótidas/metabolismo , Lesões das Artérias Carótidas/patologia , Ciclina D1/metabolismo , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Hiperplasia/prevenção & controle , Masculino , Neointima/metabolismo , Neointima/patologia , Proteínas Proto-Oncogênicas c-sis/metabolismo , Distribuição Aleatória , Ratos , Ratos WistarRESUMO
The aim of the present study was to take osteopontin (OPN) as molecular target to study its effects on injured intima model of carotid artery in rat using perivascular transfer of OPN-small interference RNA (siRNA). OPN mRNA in cultured VSMCs was quantified by real-time RT-PCR, and OPN-siRNA-002 was determined as the most sensitive sequence and used as transfected siRNA in the subsequent animal experiments. We established rat carotid arterial intima-injured model with balloon-injured method, and then perivascularly transfected OPN-siRNA-002 to study the role of OPN-siRNA in regulating several related genes including proliferating cell nuclear antigen (PCNA), transforming growth factor ß1(TGF-ß1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-14 (MMP-14), as well as its role in neointimal formation. OPN mRNA and protein decreased about 50 % with corresponding decrease in intima thickness after transfecting with specific OPN-siRNA-002 compared with Pluronic control group and OPN-SCR-siRNA group on each time point (n = 6, p < 0.001), and this inhibiting effects persisted up to 14 days after balloon injury. PCNA, TGF-ß1, MMP-2, and MMP-14 mRNA and protein correlated directly with the respective levels of OPN, suggesting its functions via regulating these downstream factors (n = 6, p < 0.001). OPN may be a potential target gene in reducing the risk for arterial restenosis after vascular intervention.
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Lesões das Artérias Carótidas/patologia , Artéria Carótida Primitiva/patologia , Neointima/patologia , Osteopontina/genética , Interferência de RNA , RNA Interferente Pequeno/genética , Animais , Lesões das Artérias Carótidas/etiologia , Artéria Carótida Primitiva/metabolismo , Proliferação de Células , Células Cultivadas , Regulação para Baixo , Expressão Gênica , Hiperplasia/etiologia , Hiperplasia/patologia , Hiperplasia/prevenção & controle , Masculino , Metaloproteinase 14 da Matriz/genética , Metaloproteinase 14 da Matriz/metabolismo , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 2 da Matriz/metabolismo , Miócitos de Músculo Liso/metabolismo , Osteopontina/metabolismo , Intervenção Coronária Percutânea/efeitos adversos , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/patologia , Complicações Pós-Operatórias/prevenção & controle , Ratos , Ratos Wistar , Lesões do Sistema Vascular/etiologia , Lesões do Sistema Vascular/patologiaRESUMO
Previous studies identified a positive feedback loop in rat vascular smooth muscle cells (VSMCs) in which early growth response factor-1 (Egr-1) binds to the osteopontin (OPN) promoter and upregulates OPN expression, and OPN upregulates Egr-1 expression via the extracellular signal-regulated protein kinase (ERK) signaling pathway. The current study examined whether transforming growth factor-beta (TGF-beta) activity contributes to Egr-1 binding to the OPN promoter, and whether other signaling pathways act downstream of OPN to regulate Egr-1 expression. ChIP assays using an anti-Egr-1 antibody showed that amplification of the OPN promoter sequence decreased in TGF-beta DNA enzyme-transfected VSMCs relative to control VSMCs. Treatment of VSMCs with PD98059 (ERK inhibitor), SP600125 (JNK inhibitor), or SB203580 (p38 MAPK inhibitor) significantly inhibited OPN-induced Egr-1 expression, and PD98059 treatment was associated with the most significant decrease in Egr-1 expression. OPN-stimulated VSMC cell migration was inhibited by SP600125 or SB203580, but not by PD98059. Furthermore, MTT assays showed that OPN-mediated cell proliferation was inhibited by PD98059, but not by SP600125 or SB203580. Taken together, the results of the current study show that Egr-1 binding to the OPN promoter is positively regulated by TGF-beta, and that the p38 MAPK, JNK, and ERK pathways are involved in OPN-mediated Egr-1 upregulation.
Assuntos
Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Regulação da Expressão Gênica , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Osteopontina/genética , Fator de Crescimento Transformador beta/metabolismo , Animais , Células Cultivadas , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Retroalimentação Fisiológica , MAP Quinase Quinase 4/antagonistas & inibidores , MAP Quinase Quinase 4/metabolismo , Músculo Liso Vascular/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Regiões Promotoras Genéticas , Inibidores de Proteínas Quinases/farmacologia , Ratos , Transdução de Sinais , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismoRESUMO
AIMS: Early growth response factor-1 (EGR-1) plays a master regulatory role in multiple cardiovascular pathological processes, such as atherosclerosis and restenosis. For investigating the possibility of using "decoy" strategy to prevent and cure vascular hyperplasia disease, we synthesized the double-stranded, cis-element, decoy oligodeoxynucleotides (ODNs) targeting EGR-1. MAIN METHODS: EGR-1 decoy ODNs were transfected into the balloon-injured arteria carotis of rat as well as primary cultures of vascular smooth muscle cells (VSMC). Changes in the thickness of the arterial intima were evaluated by hematoxylin-eosin (HE) staining. VSMC proliferation, DNA synthesis, cell cycle and apoptosis were observed via MTT assay, bromodeoxyuridine (BrdU) incorporation and flow cytometry (FCM). Changes in the expression of EGR-1, and cell cycle related genes, were detected by reverse transcriptase polymerase chain reaction (PT-PCR) and western blot. KEY FINDINGS: As a result of specific binding to EGR-1 protein, transfected EGR-1 decoy ODNs can reduce EGR-1 promoter affinity, hamper the transcriptional activation of EGR-1-dependent genes, block cell cycle progression of VSMCs, and inhibit neointimal hyperplasia. SIGNIFICANCE: Through regulating the cell cycle progression and transcription of target gene, this new "decoy" strategy targeting EGR-1 provides further experimental evidence demonstrating the effectiveness of gene therapy in the treatment of restenosis following percutaneous coronary interventions.
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Proliferação de Células/efeitos dos fármacos , Reestenose Coronária/terapia , Proteína 1 de Resposta de Crescimento Precoce/antagonistas & inibidores , Terapia Genética/métodos , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Oligonucleotídeos/farmacologia , Angioplastia Coronária com Balão/efeitos adversos , Animais , Artérias Carótidas/metabolismo , Artérias Carótidas/patologia , Doenças das Artérias Carótidas/genética , Doenças das Artérias Carótidas/metabolismo , Doenças das Artérias Carótidas/patologia , Ciclo Celular , Reestenose Coronária/patologia , Proteína 1 de Resposta de Crescimento Precoce/genética , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Hiperplasia , Masculino , Músculo Liso Vascular/lesões , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/patologia , Oligonucleotídeos/genética , Ratos , Ratos Wistar , Transcrição Gênica/efeitos dos fármacos , Transcrição Gênica/genética , TransfecçãoRESUMO
Early growth response factor-1 (Egr-1) and osteopontin (OPN) play important roles in the migration and proliferation of vascular smooth muscle cells (VSMC), but little is known about their relationship. Therefore, we transfected VSMCs with either Egr-1 cDNA, Opn cDNA, a DNA enzyme designed to target Egr-1 (ED5), or antisense Opn oligodeoxynucleotides and examined changes in Egr-1 and OPN expression at the mRNA and protein levels. OPN expression levels were increased in cells that were stably transfected with Egr-1 cDNA. By contrast, both Egr-1 and OPN expression were reduced when ED5 was transfected into Egr-1-expressing cells. Similarly, Opn transfection upregulated Egr-1 levels, while Opn anti-sense oligodeoxynucleotide transfection decreased Egr-1 expression. ChIP analysis showed that Egr-1 binds to the Opn gene promoter. Furthermore, treatment with the extracellular-regulated protein kinase (ERK) inhibitor PD98059 inhibited the upregulation of Egr-1 by OPN. We find that Egr-1 and OPN positively regulate each other in VSMCs.
Assuntos
Proteína 1 de Resposta de Crescimento Precoce/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Músculo Liso Vascular/metabolismo , Osteopontina/metabolismo , Regiões Promotoras Genéticas/genética , Transdução de Sinais , Animais , Western Blotting , Células Cultivadas , Imunoprecipitação da Cromatina , Proteína 1 de Resposta de Crescimento Precoce/antagonistas & inibidores , Proteína 1 de Resposta de Crescimento Precoce/genética , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , Flavonoides/farmacologia , Músculo Liso Vascular/citologia , Osteopontina/antagonistas & inibidores , Osteopontina/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Sequências Reguladoras de Ácido Nucleico , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transfecção , Regulação para CimaRESUMO
OBJECTIVE: To investigate the relationship between PPAR coactivator 1 (PGC-1), nuclear respiratory factor (NRF), mitochondrial transcription factor A (mtTFA) expressions of vascular smooth muscle cells (VSMC) and development of atherosclerosis in a rabbit model. METHODS: Atherosclerotic model was established by feeding the rabbits with high-fat diet for 4, 8 and 12 weeks (n = 10 each). Another 8 rabbits fed with normal diet served as normal controls. Intima-media ratio, mRNA and protein expressions of PGC-1, NRF, mtTFA and SMemb, a marker for synthetic VSMC, were detected on aorta specimens. RESULTS: With the blood lipid increased, the intima-media ratio rose from (0.031 +/- 0.010) microm up to (0.814 +/- 0.258) microm during 12 weeks. Increasing SMemb means that synthetic VSMC grew more and more. The expressions of PGC-1 became significant after 4 weeks (P < 0.01), while that of NRF-1 and mtTFA rose significantly after 8 weeks (P < 0.01). CONCLUSIONS: The PGC-NRF-mtTFA pathway might play a critical role in VSMC proliferation and development of atherosclerosis.
Assuntos
Aterosclerose/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas Mitocondriais/metabolismo , Músculo Liso Vascular/metabolismo , Fator 1 Nuclear Respiratório/metabolismo , Fatores de Transcrição/metabolismo , Animais , Aterosclerose/sangue , Aterosclerose/patologia , Modelos Animais de Doenças , Feminino , Lipídeos/sangue , Masculino , Coelhos , Transativadores/metabolismoRESUMO
BACKGROUND: Early growth response factor-1 (Egr-1) controls the gene expression involved in postangioplasty restenosis. In the present study we synthesized specific catalytic DNA targeting sequences in human Egr-1 mRNA to investigate the effects on artery balloon injury. METHODS: The catalytic DNA, ED5, was synthesized and transfected into the arterial wall of Wistar rats using the FuGENE6 transfection reagent. The animals were euthanized at day 3, 7, 14 and 21 following artery balloon injury. Serum nitric oxide (NO), nitric oxide synthase (NOS), and endothelin (ET) levels were measured before sacrifice. Histopathological changes to the arterial tissue were evaluated by H&E staining and observed via transmission electromicroscopy. Egr-1, PCNA and TGF-beta(1) expression was detected by immunohistochemistry, RT-PCR, and western-blot. RESULTS: Compared with the control groups, ED5-treated rats exhibited increased levels of both NO and NOS (p<0.05); by contrast, plasma ET levels were decreased relative to controls (p<0.05). Neointimal hyperplasia (NH) was significantly reduced and vascular smooth muscle cells (VSMCs) in the neointima exhibited a general contractile phenotype. Both protein and mRNA expression of Egr-1, PCNA, and TGF-beta(1) in the ED5-treated group were decreased at each time point (p<0.001). CONCLUSIONS: ED5 may specifically inhibit Egr-1 gene expression and reduce NH after balloon injury in rats; the latter effect may be mediated by a down-regulation of TGF-beta(1) and up-regulation of NOS to inhibit NH following balloon injury.