Integrating trans-omics, cellular experiments and clinical validation to identify ILF2 as a diagnostic serum biomarker and therapeutic target in gastric cancer.

BACKGROUND: Gastric cancer (GC) lacks serum biomarkers with clinical diagnostic value. Multi-omics analysis is an important approach to discovering cancer biomarkers. This study aimed to identify and validate serum biomarkers for GC diagnosis by cross-analysis of proteomics and transcriptomics datasets. METHODS: A cross-omics analysis was performed to identify overlapping differentially expressed genes (DEGs) between our previous aptamer-based GC serum proteomics dataset and the GC tissue RNA-Seq dataset in The Cancer Genome Atlas (TCGA) database, followed by lasso regression and random forest analysis to select key overlapping DEGs as candidate biomarkers for GC. The mRNA levels and diagnostic performance of these candidate biomarkers were analyzed in the original and independent GC datasets to select valuable candidate biomarkers. The valuable candidate biomarkers were subjected to bioinformatics analysis to select those closely associated with the biological behaviors of GC as potential biomarkers. The clinical diagnostic value of the potential biomarkers was validated using serum samples, and their expression levels and functions in GC cells were validated using in vitro cell experiments. RESULTS: Four candidate biomarkers (ILF2, PGM2L1, CHD7, and JCHAIN) were selected. Their mRNA levels differed significantly between tumor and normal tissues and showed different diagnostic performances for GC, with areas under the receiver operating characteristic curve (AUROCs) of 0.629-0.950 in the TCGA dataset and 0.736-0.840 in the Gene Expression Omnibus (GEO) dataset. In the bioinformatics analysis, only ILF2 (interleukin enhancer-binding factor 2) gene levels were associated with immune cell infiltration, some checkpoint gene expression, chemotherapy sensitivity, and immunotherapy response. Serum levels of ILF2 were higher in GC patients than in controls, with an AUROC of 0.944 for the diagnosis of GC, and it was also detected in the supernatants of GC cells. Knockdown of ILF2 by siRNA significantly reduced the proliferation and colony formation of GC cells. Overexpression of ILF2 significantly promotes the proliferation and colony formation of gastric cancer cells. CONCLUSIONS: Trans-omics analysis of proteomics and transcriptomics is an efficient approach for discovering serum biomarkers, and ILF2 is a potential diagnostic biomarker and therapeutic target of gastric cancer.

Continuous Tunable Energy Band Tailoring Boosts Extending the Sensing of the Waveband Based on (InxGa1-x)2O3 Solar-Blind Photodetectors.

Rising wide bandgap semiconductor gallium oxide (Ga2O3) displays huge potential in performing solar-blind photodetection, with constraint in narrow detection wavebands in nature, whereas bandgap modulation through the introduction of exotic atoms into Ga2O3 has an essential effect on the tunable performance of photodetectors and the detection waveband. Here, a novel method for the preparation of (InxGa1-x)2O3 alloy films is proposed, and the continuous tuning of the bandgap in the range of 3.70-4.99 eV is achieved by varying the In-doping content. Alloy-based metal-semiconductor-metal photodetectors were fabricated, achieving a peak responsivity between 254 and 295 nm, superior performance compared to Ga2O3 photodetectors, with a photo-to-dark current ratio as high as 106, and a better optical image-sensing capability. This study offers new insight for high-performance detection of full solar-blind waveband ultraviolet light.

The Index sAGP is Valuable for Distinguishing Atypical Hepatocellular Carcinoma from Atypical Benign Focal Hepatic Lesions.

Purpose: The differential diagnosis of atypical hepatocellular carcinoma (aHCC) and atypical benign focal hepatic lesions (aBFHL) usually depends on pathology. This study aimed to develop non-invasive approaches based on conventional blood indicators for the differential diagnosis of aHCC and aBFHL. Patients and Methods: Hospitalized patients with pathologically confirmed focal hepatic lesions and their clinical data were retrospectively collected, in which patients with HCC with serum alpha-fetoprotein (AFP) levels of ≤200 ng/mL and atypical imaging features were designated as the aHCC group (n = 224), and patients with benign focal hepatic lesions without typical imaging features were designated as the aBFHL group (n = 178). The performance of indexes (both previously reported and newly constructed) derived from conventional blood indicators by four mathematical operations in distinguishing aHCC and aBFHL was evaluated using the receiver operating characteristic (ROC) curve and diagnostic validity metrics. Results: Among ten previously reported derived indexes related to HCC, the index GPR, the ratio of γ-glutamyltransferase (GGT) to platelet (PLT), showed the best performance in distinguishing aHCC from aBFHL with the area under ROC curve (AUROC) of 0.853 (95% CI 0.814-0.892), but the other indexes were of little value (AUROCs from 0.531 to 0.700). A new derived index, sAGP [(standardized AFP + standardized GGT)/standardized PLT], was developed and exhibited AUROCs of 0.905, 0.894, 0.891, 0.925, and 0.862 in differentiating overall, BCLC stage 0/A, TNM stage I, small, and AFP-negative aHCC from aBFHL, respectively. Conclusion: The sAGP index is an efficient, simple, and practical metric for the non-invasive differentiation of aHCC from aBFHL.

Evolution and Trends of Aptamers in Cancer Research: A Bibliometric Analysis of the Literature over the Last Decade.

OBJECTIVE: Aptamers are increasingly applied in cancer research. Here, we have performed the first bibliometric analysis to demonstrate the evolution of aptamers in cancer research over the past decade and to reveal future trends. METHOD: Original articles and reviews on aptamers in cancer research published from 2013 to 2022 were retrieved from the Web of Science Core Collection database. VOSviewer, CiteSpace, and R software were used for bibliometric analysis of the literature and visualization of the results. RESULTS: A total of 1627 eligible publications were analyzed. Annual and cumulative publications have been found to be steadily increased. China was the most productive country (884 publications) and Hunan University was the most productive institution (97 publications). The United States had the highest level of international collaboration (betweenness centrality = 0.55). Wei-Hong Tan was the most productive author (68 publications) and Craig Tuerk was the most cited author (387 citations). Analytical Chemistry and Biosensors and Bioelectronics were the most influential journals in this field. Three major themes were identified: aptamer selection techniques, aptamer-targeted drug delivery, and aptasensors for cancer detection. The research hotspots have shifted from aptamer selection, targeted drug delivery, molecular imaging, and biomarker detection to electrochemical aptasensors and therapeutic applications. The future may focus on high specificity and affinity in aptamer selection, aptasensor fabrication, aptamer- targeted drug delivery, and therapeutic aptamer development. CONCLUSION: The field of aptamers in cancer research has been steadily developing over the past decade, and future research may focus on aptamer application in cancer detection and therapy and the improvement of aptamer selection.

Highly Monochromatic Ultraviolet LED Based on the SnO2 Microwire Heterojunction Beyond Dipole-Forbidden Band-Gap Transition.

SnO2 has been extensively applied in the fields of optoelectronic devices because of its large band gap, high exciton binding energy, and outstanding optical/electrical properties. However, its applications in ultraviolet light-emitting diodes (LEDs) are still hindered by the dipole-forbidden rule. Herein, the dipole-forbidden rule can be conquered by synthesizing Sb-incorporated SnO2 microwires (SnO2:Sb MWs), which are examined by ultraviolet photoluminescence emitting at 363.2 nm and a line width of 11.3 nm. Subsequently, a highly monochromatic ultraviolet light-emitting diode (LED) based on a SnO2:Sb MW heterojunction was constructed with a p-GaN film serving as the hole supplier. In the LED, the presence of a MgO intermediate layer can modulate carrier transport and recombination path, thus achieving band-edge optical transition in the SnO2:Sb MW. As the LED is modified using Ag nanowires, electrical properties, especially for the hole injection efficiency, were dramatically boosted, contributing significantly to the device high brightness. The LED emits at 365.9 nm and a line width of 12.4 nm. Therefore, we have realized a high-brightness and narrow-band ultraviolet LED with the shortest peak wavelength never seen in previously reported SnO2 LEDs. This work will promote the potential applications of low-dimensional SnO2 optoelectronic devices and provide an effective exemplification to overcome the dipole-forbidden rule in metal-oxide materials with "forbidden" energy gaps.

Bibliometric analysis of aptamer-conjugated nanoparticles for diagnosis in the last two decades.

Objective.Aptamer-conjugated nanoparticles for diagnosis have recently gained increasing attention. Here, we performed a bibliometric analysis to provide an overview of this field over the past two decades.Methods. The terms 'aptamer, nanoparticles and diagnosis' were used to search for relevant original articles published in English from 2003 to 2022 in the Web of Science database. VOSviewer and CiteSpace software were employed to analyze the development process, knowledge structure, research hotspots, and potential trends in the field of aptamer-conjugated nanoparticles for diagnosis.Results. A total of 1076 original articles were retrieved, with a rapid increase in the annual output and citation. The journal 'Biosensors and Bioelectronics' has contributed the most in this field, and the most influential researcher, institution and country were Weihong Tan, the Chinese Academy of Sciences, China, respectively. Gold nanoparticles and quantum dots were the most used, but in the past three years, research hotspots focused on carbon dots and graphene quantum dots. Diagnostic directions primarily focused on cancer. The most used strategy was label-free electrochemical detection, but in the past two years, colorimetric analysis and fluorescence imaging emerged as hot topics.Conclusion.The bibliometric analysis reveals a rapid increase in the research on aptamer-conjugated nanoparticles for diagnosis, major contributors at the levels of journals, authors, institutions, and countries, and research preferences in diagnostic objects, nanoparticle types, and detection methods, as well as the evolution of research hotspots and future trends.

Improving assessment quality of soil natural attenuation capacity at the point and regional scales.

Soil natural attenuation capacity (NAC) is an important ecosystem service that maintains a clean environment for organisms in the soil, which in turn supports other services. However, spatially varying indicator weights were rarely considered in the traditionally-used soil NAC assessment model (e.g., ecosystem-service performance model) at the point scale. Moreover, in the spatial simulation of soil NAC, the traditionally-used geostatistical models were usually susceptible to spatial outliers and ignored valuable auxiliary information (e.g., land-use types). This study first proposed a novel soil NAC assessment method based on the ecosystem-service performance model and moving window-entropy weight method (MW-EW) (NACMW-EW). Next, NACMW-EW was used to assess soil NAC in a typical area in Guixi City, China, and further compared with the traditionally-used NACtra and NACEW. Then, robust sequential Gaussian simulation with land-use types (RSGS-LU) was established for the spatial simulation of NACMW-EW and compared with the traditionally-used SGS, SGS-LU, and RSGS. Last, soil NAC's spatial uncertainty was evaluated based on the 1000 realizations generated by RSGS-LU. The results showed that: (i) MW-EW effectively revealed the spatially varying indicator weights but EW couldn't; (ii) NACMW-EW obtained more reasonable results than NACtra and NACEW; (iii) RSGS-LU (RMSE = 0.118) generated higher spatial simulation accuracy than SGS-LU (RMSE = 0.123), RSGS (RMSE = 0.132), and SGS (RMSE = 0.135); and (iv) the relatively high (P[NACMW-EW(u) > 0.57] ≥ 0.95) and low (P[NACMW-EW(u) > 0.57] ≤ 0.05) threshold-exceeding probability areas were mainly located in the south and east of the study area, respectively. It is concluded that the proposed methods were effective tools for soil NAC assessment at the point and regional scales, and the results provided accurate spatial decision support for soil ecosystem service management.

Identifying the potential soil pollution areas derived from the metal mining industry in China using MaxEnt with mine reserve scales (MaxEnt_MRS).

Identifying the potential soil pollution areas derived from the metal mining industry usually requires extensive field investigation and laboratory analysis. Moreover, the previous studies mainly focused on a single or a few mining areas, and thus couldn't provide effective spatial decision support for controlling soil pollution derived from the metal mining industry at the national scale. This study first conducted a literature investigation and web crawler for the relevant information on the metal mining areas in China. Next, MaxEnt with mine reserve scales (MaxEnt_MRS) was proposed for spatially predicting the probabilities of soil pollution derived from the metal mining industry in China. Then, MaxEnt_MRS was compared with the basic MaxEnt. Last, the potential soil pollution areas were identified based on the pollution probabilities, and the relationships between the soil pollution probabilities and the main environmental factors were quantitatively assessed. The results showed that: (i) MaxEnt_MRS (AUC = 0.822) obtained a better prediction effect than the basic MaxEnt (AUC = 0.807); (ii) the areas with the soil pollution probabilities higher than 54% were mainly scattered in the eastern, south-western, and south-central parts of China; (iii) GDP (45.7%), population density (30.1%), soil types (15.5%), average annual precipitation (3.9%), and land-use types (3.1%) contributed the most to the prediction of the soil pollution probabilities; and (iv) the soil pollution probabilities in the areas with all the following conditions were higher than 54%: GDP, 7600-2612670 thousand yuan/km2; population density, 152-551 people/km2; precipitation, 924-2869 mm/year; soil types, Ferralisols or Luvisols; and land-use types, townland, mines, and industrial areas. The above-mentioned results provided effective spatial decision support for controlling soil pollution derived from the metal mining industry at the national scale.

Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma.

Ferroptosis is a newly characterized form of iron-dependent non-apoptotic cell death, which is closely associated with cancer progression. However, the functions and mechanisms in regulation of escaping from ferroptosis during hepatocellular carcinoma (HCC) progression remain unknown. In this study, we reported that the RNA binding motif single stranded interacting protein 1 (RBMS1) participated in HCC development，and functioned as a regulator of ferroptosis. Clinically, the downregulation of RBMS1 occurred in HCC tissues, and low RBMS1 expression was associated with worse HCC patients survival. Mechanistically, RBMS1 overexpression inhibited HCC cell growth by attenuating the expression of glutathione peroxidase 4 (GPX4)and further facilitated ferroptosis in vitro and in vivo. More importantly, a novel circIDE (hsa_circ_0000251) was identified to elevate RBMS1 expression via sponging miR-19b-3p in HCC cells. Collectively, our findings established circIDE/miR-19b-3p/RBMS1 axis as a regulator of ferroptosis, which could be a promising therapeutic target and prognostic factor.

Ultraviolet Exciton-Polariton Light-Emitting Diode in a ZnO Microwire Homojunction.

Low-dimensional ZnO, possessing well-defined side facets and optical gain properties, has emerged as a promising material to develop ultraviolet coherent light sources. However, the realization of electrically driven ZnO homojunction luminescence and laser devices is still a challenge due to the absence of a reliable p-type ZnO. Herein, the sample of p-type ZnO microwires doped by Sb (ZnO:Sb MWs) was synthesized individually. Subsequently, the p-type conductivity was examined using a single-MW field-effect transistor. Upon optical pumping, a ZnO:Sb MW showing a regular hexagonal cross-section and smooth sidewall facets can feature as an optical microcavity, which is evidenced by the achievement of whispering-gallery-mode lasing. By combining an n-type ZnO layer, a single ZnO:Sb MW homojunction light-emitting diode (LED), which exhibited a typical ultraviolet emission at a wavelength of 379.0 nm and a line-width of approximately 23.5 nm, was constructed. We further illustrated that strong exciton-photon coupling can occur in the as-constructed p-ZnO:Sb MW/n-ZnO homojunction LED by researching spatially resolved electroluminescence spectra, contributing to the exciton-polariton effect. Particularly, varying the cross-sectional dimensions of ZnO:Sb wires can further modulate the exciton-photon coupling strengths. We anticipate that the results can provide an effective exemplification to realize reliable p-type ZnO and tremendously promote the development of low-dimensional ZnO homojunction optoelectronic devices.

Value of growth arrest lines for predicting treatment effect on children with distal tibial epiphysis fractures.

Objective: This study aims to explore whether growth arrest lines can predict epiphyseal fracture healing. Method: The data of 234 children with distal tibial epiphysis fractures treated in our hospital from February 2014 to February 2022 were retrospectively analyzed. Imaging data were examined to record epiphyseal grade, fracture type, and the time to appearance of growth arrest lines. Follow-up data were retrieved to record treatment results (i.e., malunion, premature closure, or bone bridge formation). Results: There was a significant difference in the time to appearance of growth arrest lines between patients with epiphyseal grade 0-1 and grade 2-3 (P < 0.05) and between patients with normal healing and patients with a bone bridge (P < 0.05). Among patients with normal healing, there were no significant differences in the time to appearance of growth arrest lines between men and women and between patients with and without surgery (P > 0.05). There was a significant difference in the time to appearance of growth arrest lines between patients with different Salter-Harris fracture types (P < 0.05). Conclusion: For patients with epiphyseal grade 0-1, the time to appearance of growth arrest lines could be useful for predicting the treatment result of a distal tibial epiphyseal fracture.

Exciton-polariton light-emitting diode based on a single ZnO superlattice microwire heterojunction with performance enhanced by Rh nanostructures.

One-dimensional (1D) wirelike superlattice micro/nanostructures have received considerable attention for potential applications due to their versatility and capability for modulating optical and electrical characteristics. In this study, 1D superlattice microwires (MWs), which are made of undoped ZnO and Ga-doped ZnO with periodic and alternating crystalline layers (ZnO/ZnO:Ga), were synthesized individually. Under optical excitation, a series of resonance peaks in the photoluminescence spectrum can be ascribed to polariton emission, which originates from the coupling interaction of the 1D photonic crystal and confined excitons along the wire direction. Using a p-type GaN layer as the hole transport layer, a kind of waveguide light source based on an individual ZnO/ZnO:Ga superlattice MW was proposed and constructed. By analysing the spatially resolved electroluminescence spectra, the observed multipeak was ascribed to exciton-polariton emission with a vacuum Rabi splitting of about 275 meV. Cladding with Rh nanostructures gives rise to appropriate ultraviolet plasmons, and the Rabi splitting energy of our device was enhanced up to 413 meV. The exciton-polariton properties were further examined using angle-resolved electroluminescence measurements. Therefore, individual superlattice MWs can act as optical microresonators to achieve photon-exciton coupling with a large Rabi splitting energy. The experimental results indicate that an individual ZnO/ZnO:Ga superlattice MW can be generally used in developing exciton-polariton luminescence/lasing light sources, particularly for constructing low-threshold/thresholdless lasers toward pragmatic applications.

TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway.

BACKGROUND: Hepatocellular carcinoma is one of the most common and deadly cancers. The aim of this study was to elucidate the role of tRNA methyltransferase 6 (TRMT6) during HCC progression. METHODS: The role of TRMT6 in the progression and prognosis of HCC was confirmed by analysis of online databases and clinical human samples. The effects of up-regulation or down-regulation of TRMT6 on HCC cell proliferation and PI3K/AKT pathway-related protein expressions were verified. The molecular mechanism was investigated in vivo by constructing subcutaneous xenograft tumor model. RESULTS: TRMT6 was overexpressed in HCC tissues and associated with Tumour-Node-Metastasis (TNM) stage, primary tumor (T) and regional lymph node (N) classification. TRMT6 expressions in HCC cell lines were higher than that in normal liver cell. TRMT6 overexpression can promote HCC cell proliferation, increase the number of S phase cells. Interference with TRMT6 reduced the PI3K/AKT pathway-related protein expressions, and was reversed by the addition of IGF1. Interference with TRMT6 inhibited tumor growth in vivo and was related to PI3K/AKT pathway. CONCLUSIONS: Overexpression of TRMT6 promote HCC cell proliferation in vivo and in vitro through PI3K/AKT/mTOR axis, which provides a potential choice for the treatment of HCC in clinical practice.

Electrically driven whispering-gallery-mode microlasers in an n-MgO@ZnO:Ga microwire/p-GaN heterojunction.

In emerging miniaturized applications, semiconductor micro/nanostructures laser devices have drawn great public attentions of late years. The device performances of micro/nanostructured microlasers are highly restricted to the different reflective conditions at various side surfaces of microresonators and junction interface quality. In this study, an electrically driven whispering-gallery-mode (WGM) microlaser composed of a Ga-doped ZnO microwire covered by a MgO layer (MgO@ZnO:Ga MW) and a p-type GaN substrate is illustrated experimentally. Incorporating a MgO layer on the side surfaces of ZnO:Ga MWs can be used to reduce light leakage along the sharp edges and the ZnO:Ga/GaN interface. This buffer layer incorporation also enables engineering the energy band alignment of n-ZnO:Ga/p-GaN heterojunction and manipulating the current transport properties. The as-constructed n-MgO@ZnO:Ga MW/p-GaN heterojunction device can emit at an ultraviolet wavelength of 375.5 nm and a linewidth of about 25.5 nm, achieving the excitonic-related recombination in the ZnO:Ga MW. The broadband spectrum collapsed into a series of sharp peaks upon continuous-wave (CW) operation of electrical pumping, especially for operating current above 15.2 mA. The dominant emission line was centered at 378.5 nm, and the line width narrowed to approximately 0.95 nm. These sharp peaks emerged from the spontaneous emission spectrum and had an average spacing of approximately 5.5 nm, following the WGM cavity modes. The results highlight the significance of interfacial engineering for optimizing the performance of low-dimensional heterostructured devices and shed light on developing future miniaturized microlasers.

Electrically driven single microwire-based single-mode microlaser.

Engineering the lasing-mode oscillations effectively within a laser cavity is a relatively updated attentive study and perplexing issue in the field of laser physics and applications. Herein, we report a realization of electrically driven single-mode microlaser, which is composed of gallium incorporated zinc oxide microwire (ZnO:Ga MW) with platinum nanoparticles (PtNPs, d ~ 130 nm) covering, a magnesium oxide (MgO) nanofilm, a Pt nanofilm, and a p-type GaN substrate. The laser cavity modes could resonate following the whispering-gallery mode (WGM) among the six side surfaces by total internal reflection, and the single-mode lasing wavelength is centered at 390.5 nm with a linewidth of about 0.18 nm. The cavity quality factor Q is evaluated to about 2169. In the laser structure, the usage of Pt and MgO buffer layers can be utilized to engineer the band alignment of ZnO:Ga/GaN heterojunction, optimize the p-n junction quality and increase the current injection. Thus, the well-designed device structure can seamlessly unite the electron-hole recombination region, the gain medium, and optical microresonator into the PtNPs@ZnO:Ga wire perfectly. Such a single MW microlaser is essentially single-mode regardless of the gain spectral bandwidth. To study the single-mode operation, PtNPs working as superabsorber can engineering the multimode lasing actions of ZnO:Ga MWs even if their dimensions are typically much larger than that of lasing wavelength. Our findings can provide a straightforward and effective scheme to develop single-mode microlaser devices based on one-dimensional wire semiconductors.

The construction of molecularly imprinted electrochemical biosensor for selective glucose sensing based on the synergistic enzyme-enzyme mimic catalytic system.

It is generally accepted that glucose oxidase (GOx) shows unique specificity in ß-d-glucose catalysis. However, it has been found that GOx can catalyze diverse monosaccharides. Therefore, the sensing accuracy for glucose biosensors using GOx as probes will be largely compromised by the presence of other monosaccharides. Herein, multifunctional bi-nanospheres (Fe3O4@Au NCs), which show both peroxidase-like and catalase-like catalytic activities in different working conditions, are successfully constructed and served as desirable platform with huge surface area for the immobilization of large amount of GOx probes. In acidic environment, hydroxyl radicals could be generated via the cascaded catalysis of ß-d-glucose by Fe3O4@Au-GOx, and then employed to initiate the polymerization of boric acid derivative to prepare molecularly imprinted polymers (MIPs) on the surface of GOx using ß-d-glucose as template. Then, the molecularly imprinted GOx are immobilized on the surface of highly oriented pyrolytic graphite (HOPG) electrode and an electrochemical biosensor (Fe3O4@Au-GOx-HOPG) for glucose sensing is successfully obtained. Interestingly, the as-prepared biosensors could selectively detect glucose in the range of 10.0 µM - 5.0 mM with a LOD = 5.0 µM with the help of MIPs, which is comparable or better than other glucose sensors reported recently.

Heavy metal accumulation in the surrounding areas affected by mining in China: Spatial distribution patterns, risk assessment, and influencing factors.

Previous studies about heavy metal (HM) accumulation in the surrounding areas affected by mining mainly focused on a single or just a few mining areas. However, these studies could not provide adequate information supporting HM controls in soils at the national scale. This study first conducted a literature investigation and collected HM data in mining areas in China from 263 pieces of published literature. Then, geo-accumulation index (Igeo), ecological risk index (ER), and health risk assessment model were adopted to evaluate their HM pollution, ecological risks, and health risks, respectively. Finally, Geodetector and Pearson correlation coefficients were used to explore the relationships between the spatial distribution patterns of HMs in soils and their influencing factors. Results showed that: (i) the average concentrations of Cd, Hg, Pb, Zn, Cu, As, Ni, and Cr were 5.4, 1.2, 335.3, 496.1, 105.8, 55.0, 42.6, and 72.4 mg kg-1, respectively, in the surrounding areas affected by mining in China; Cd pollution in soils (Igeo = 2.9) was most severe; Cd (ERCd > 320) and Hg (ERHg > 320) were the main ecological risk factors; (ii) among the selected factors, mine types, clay content, soil organic carbon, and precipitation with the highest relative importance for the spatial distribution patterns of the HMs; (iii) HM accumulation were inversely proportional to soil pH, and were proportional to clay content, precipitation, and temperature; (iv) As, Cd, Hg, Pb, and Ni should be selected as the HMs to be controlled preferentially; (v) priority attention should be given to mining areas in Central South China, Southwest China, Liaoning province, and Zhejiang province; (vi) special attention should be given to mining areas of antimony, tin, tungsten, molybdenum, manganese, and lead­zinc. The above results provided crucial information for HM control in the areas affected by mining at the national scale.

Facile construction of hierarchical Co3S4/CeO2 heterogeneous nanorod array on cobalt foam for electrocatalytic overall water splitting.

Herein, a hierarchical Co3S4/CeO2 nanorod array on cobalt foam (Co3S4/CeO2-CF) was successfully constructed via a facile one-step hydrothermal method. The fabrication of Co3S4/CeO2-CF was confirmed by X-ray diffraction, scanning electron microscope (SEM), high resolution transmission electron microscope (HRTEM). It is observed that CeO2 nanorod was fully covered with Co3S4 nanosheets, forming a hierarchical core-shell nanostructure. Furthermore, CeO2 and Co3S4 were doped with each other during the one-step hydrothermal process, forming a heterogeneous Co3S4/CeO2 nanostructure. Density functional theory (DFT) calculations suggest that the introduction of CeO2 in Co3S4 is effective in reducing the free energy barrier of OER process. To achieve current density of 10 mA cm-2, only small overpotentials of 74.9 mV and 213 mV are required for HER and OER in 1.0 M KOH solution, respectively. In particular, the Co3S4/CeO2-CF based electrolysis cell for overall water splitting only needs an output voltage of 1.64 V in the alkaline medium, lower than that of Pt/C-RuO2-based electrolysis cells (1.70 V). Such hierarchical heterogeneous catalyst also shows ultra-stable catalytic activity. Therefore, with the favorable heterointerfaces and hierarchical structures, Co3S4/CeO2-CF could be a promising bifunctional electrocatalyst for overall water splitting and this study may also provide a facile method for the preparation of hierarchical heterogeneous nanostructured materials.

The role of circRNA derived from RUNX2 in the serum of osteoarthritis and its clinical value.

BACKGROUND: Circular RNA (circRNA) has been shown to affect the pathological process of osteoarthritis (OA) and is expected to become a potential marker for disease diagnosis. This study aimed to investigate the association between circRNA derived from the gene of runt-related transcription factor 2 (RUNX2) and OA risk. METHODS: The expression profile of RUNX2-derived circRNAs in serum of OA patients was detected. Then, the cytological localization of screened differential circRNAs was studied. Luciferase (LUC) reporter assay was used to identify the microRNA (miRNA) sponge capacity of the circRNAs. Bioinformatics analysis was used to construct the functional pathway of this circRNA-miRNAs network. And then, the diagnostic value of RUNX2-derived circRNAs in OA was evaluated. RESULTS: RUNX2-derived hsa_circ_0005526 (circ_RUNX2) is significantly highly expressed in OA serum and mainly located in the cytoplasm within the cartilage cell by sponging multiple miRNAs (miR-498, miR-924, miR-361-3p, and miR-665). Bioinformatics analysis showed ECM-receptor interaction pathway ranked the most significant pathway of circ_RUNX2-miRNAs regulatory network in KEGG database. The ROC curve showed that there may be good diagnostic value of serum circ_RUNX2 in OA. CONCLUSION: RUNX2-derived circ_RUNX2 may be involved in OA development via ECM-receptor interaction pathways and may be used as potential clinical indicator of OA.

A molecularly imprinted nanoreactor with spatially confined effect fabricated with nano-caged cascaded enzymatic system for specific detection of monosaccharides.

Glucose oxidase (GOx), traditionally regarded as an oxidoreductase with high ß-D-glucose specificity, has been widely applied as sensing probe for ß-D-glucose detection. However, it is found that the specificity of GOx is not absolute and GOx cannot decern ß-D-glucose among its isomers such as xylose, mannose and galactose. The existence of the other monosaccharides in sensing system could compromise the sensitivity for ß-D-glucose, therefore, it is of great urgency to achieve the highly specific catalytic performance of GOx. Herein, porous metal-organic frameworks (MOF) are prepared as the host matrix for immobilization of both GOx and bovine hemoglobin (BHb), obtained a cascaded catalytic system (MOF@GOx@BHb) with both enhanced GOx activity and peroxidase-like activity owing to the spatially confined effect. Then, using ß-D-glucose as both template molecules and substances, hydroxyl radicals are produced continuously and applied for initiating the polymerization of molecular imprinting polymers (MIPs) on the surface of MOF@GOx@BHb. Impressively, the obtaining molecularly imprinted GOx (noted as MOF@GOx@BHb-MIPs) achieves the highly sensitive and specific detection of ß-D-glucose in the concentration range of 0.5-20 µM with the LOD = 0.4 µM (S/N = 3) by colorimetry. Similarly, MOF@GOx@BHb-MIPs are subsequently obtained using mannose, xylose and galactose as template molecules, respectively, and also show satisfied specific catalytic activity towards corresponding templates, indicating the effectiveness of the proposed strategy to achieve highly specific catalytic performance of GOx.