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BACKGROUND: Hemolymphangioma is a very rare benign tumor in clinical practice caused by abnormalities of the vasculature. Its clinical features are often atypical, and it is easy to miss and misdiagnose. When the time of nuclear magnetic T1 is significantly reduced, the diagnosis of hemangioma should be considered. Therefore, we report this case in the hope of raising clinicians' awareness of the disease. CASE SUMMARY: A 37-year-old man presented with a giant retroperitoneal hemolymphangioma. Computed tomography and magnetic resonance imaging indicated the possibility of a large perirenal lymphatic cyst. The postoperative pathological diagnosis is retroperitoneal hemolymphangioma. The patient underwent surgical excision after adequate drainage. The postoperative recovery was smooth and there were no complications. There was no recurrence during half a year of follow-up. CONCLUSION: This case reiterates that large retroperitoneal cystic masses with significantly shortened nuclear T1 time should be considered hemolymphangioma. Specific clinical basis and experience for the diagnosis and treatment of these diseases is necessary.
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The interaction between nanoscale copper oxides (nano-CuOs) and soil matrix significantly affects their fate and transport in soils. This study investigates the retention of nano-CuOs and Cu2+ ions in ten typical agricultural soils by employing the Freundlich adsorption model. Retention of nano-CuOs and Cu2+ in soils was well fitted by the Freundlich model. The retention parameters (KD, KF, and N) followed an order of CuO NTs > CuO NPs > Cu2+, highlighting significant impact of nano-CuOs morphology. The KF and N values of CuO NPs/Cu2+ were positively correlated with soil pH and electrical conductivity (EC), but exhibited a weaker correlation for CuO NTs. Soil pH and/or EC could be used to predict KF and N values of CuO NPs or CuO NTs, with additional clay content should be included for Cu2+.The different relationship between retention parameters and soil properties may suggest that CuO NTs retention mainly caused by agglomeration, whereas adsorption and agglomeration were of equal importance to CuO NPs. The amendment of Ca2+ at low and medium concentration promoted retention of nano-CuOs in alkaline soils, but reduced at high concentration. These findings provided critical insights into the fate of nano-CuOs in soil environments, with significant implications for environmental risk assessment and soil remediation strategies.
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Agricultura , Cobre , Poluentes do Solo , Solo , Cobre/química , Solo/química , Poluentes do Solo/química , Concentração de Íons de Hidrogênio , Adsorção , Nanopartículas Metálicas/química , Condutividade Elétrica , Tamanho da PartículaRESUMO
Stereo matching and instrument segmentation of laparoscopic surgical scenarios are key tasks in robotic surgical automation. Many researchers have been studying the two tasks separately for stereo matching and instrument segmentation. However, the relationship between these two tasks is often neglected. In this paper, we propose a model framework for multi-tasking with complementary functions for stereo matching and surgical instrument segmentation (MCF-SMSIS). We aim to complement the features of instrument prediction segmentation to the parallax matching block of stereo matching. We also propose two new evaluation metrics (MINPD and MAXPD) for assessing how well the parallax range matches the migrated domain when the model used for the stereo matching task undergoes domain migration. We performed stereo matching experiments on the SCARED , SERV-CT dataset as well as instrumentation segmentation experiments on the AutoLaparo dataset. The results demonstrate the effectiveness of the proposed method. In particular, stereo matching supplemented with instrument features reduced EPE, >3px and RMSE Depth in the surgical instrument section by 9.5%, 12.7% and 6.51%, respectively. The instrumentation segmentation performance also achieves a DSC value of 0.9233. Moreover, MCF-SMSIS takes only 0.14 s to infer a set of images. The model code and model weights for each stage are available from https://github.com/wurenkai/MCF-SMSIS.
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Procedimentos Cirúrgicos Robóticos , Humanos , Procedimentos Cirúrgicos Robóticos/métodos , Laparoscopia , Imageamento Tridimensional/métodos , AlgoritmosRESUMO
KEY MESSAGE: MePMTR1 is involved in plant development and production as well as photosynthesis in plant. Melatonin is widely involved in plant growth and development as well as stress responses. Compared with the extending studies of melatonin in stress responses, the direct link between melatonin and plant development in the whole stages remains unclear. With the identification of phytomelatonin receptor PMTR1 in plants, melatonin signalling is becoming much clearer. However, the function of MePMTR1 in tropical crop cassava remains elusive. In this study, we found that overexpression of MePMTR1 showed larger biomass than wild type (WT), including higher number and area of leaves, weight, and accompanying with higher photosynthetic efficiency. Consistently, exogenous melatonin accelerated photosynthetic rate in Arabidopsis. In addition, MePMTR1-overexpressed plants exhibited more resistance to dark-induced senescence compared with WT, demonstrated by higher chlorophyll, lower hydrogen peroxide and superoxide content. In summary, this study illustrated that melatonin and its receptor regulate growth, development and senescence in plants, highlighting the potential application of melatonin and its receptor in improving crop yield and photosynthesis.
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Arabidopsis , Regulação da Expressão Gênica de Plantas , Manihot , Melatonina , Fotossíntese , Plantas Geneticamente Modificadas , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Melatonina/metabolismo , Manihot/genética , Manihot/crescimento & desenvolvimento , Manihot/metabolismo , Receptores de Melatonina/metabolismo , Receptores de Melatonina/genética , Luz , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Folhas de Planta/metabolismo , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Clorofila/metabolismo , Escuridão , Peróxido de Hidrogênio/metabolismoRESUMO
Grazing is the most extensive land use in grassland worldwide, wherein the soil microbiome is known to support multiple ecosystem functions. Yet, the experimental impact of livestock grazing and dung deposits on the soil microbiome in degraded grassland remains poorly understood. We examined the effects of sheep dung depositions on the bacterial and fungal microbiome of two grasslands: non-degraded and degraded (long-term overgrazing) in northern China. Specifically, sheep dung was experimentally added to the soil and its effects on the soil microbial community were determined 3 months later (corresponding to livestock excreta deposited throughout the entire growing season of grassland, June to September). Our results showed that sheep dung additions showed negative effects on the soil microbiome of already degraded grassland, while with a diminished impact on the non-degraded grassland. In particular, dung deposition decreased soil microbial Shannon index, notably significantly reducing fungal diversity in degraded grassland. Moreover, sheep dung deposition modifies soil bacterial community structure and diminishes bacterial community network complexity. The alteration of soil pH caused by sheep dung deposition partially explains the decline in microbial diversity in degraded grassland. However, sheep dung did not alter the relative abundance and community composition of bacterial and fungal dominant phyla either in the non-degraded or in the degraded grassland. In conclusion, the short-term deposition of sheep dung exerted a detrimental influence on the microbial community in degraded grassland soil. It contributes new experimental evidence regarding the adverse effects of livestock grazing, particularly through dung deposition, on the soil microbiome in degraded grassland. This knowledge is crucial for guiding managers in conserving the soil microbiome in grazed grasslands.
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Fezes , Pradaria , Microbiota , Microbiologia do Solo , Animais , Ovinos/microbiologia , Fezes/microbiologia , China , Fungos , Bactérias/classificação , Bactérias/metabolismo , Solo/químicaRESUMO
The increasing use of copper oxide nano particles (nCuO) as nano-fertilizers and pesticides have raised concerns over their impact on soil environment and agricultural products. In this study, two nCuO with different shapes, namely spherical nCuO (CuO NPs) and tubular nCuO (CuO NTs), were selected to investigate their bioavailability and toxicity to pakchoi in two soils with different properties. At the meantime, CuO bulk particles (CuO BPs) and Cu(NO3)2 were used for comparison. Results showed that all the Cu treatments increased the DTPA extractable (DTPA-Cu) concentrations in GD soil (acidic) more than in HN soil (alkaline). The DTPA-Cu concentrations increased in the order of Cu(NO3)2 ≈ CuO NPs > CuO BPs ≈ CuO NTs in GD soil and Cu(NO3)2 > CuO NPs > CuO BPs ≈ CuO NTs in HN soil. While for the contents of Cu in the aerial parts of pakchoi, the order is CuO NPs > Cu(NO3)2 > CuO NTs ≈ CuO BPs in GD soil and CuO NPs ≈ Cu(NO3)2 > CuO BPs ≈ CuO NTs in HN soil. Only CuO NPs reduced pakchoi biomass in GD soil. There are no significant difference among CuO NPs, CuO BPs, and Cu(NO3)2 in reducing the chlorophyll contents in pakchoi in HN soil, whereas in GD soil, CuO NPs and CuO BPs led to significantly lower chlorophyll contents in pakchoi compared to Cu(NO3)2. Additionally, CuO NPs and Cu(NO3)2 increased Mn and Mo in pakchoi leaf in HN soil, while increased Zn in pakchoi leaf in GD soil. These results indicated that CuO NPs showed higher or comparable toxicity and bioavailability to pakchoi compared with Cu(NO3)2 depending on soil properties, and nCuO are more easily to be transferred from roots to the aerial parts than CuO BPs and Cu(NO3)2.
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Brassica , Nanopartículas Metálicas , Nanopartículas , Cobre/toxicidade , Disponibilidade Biológica , Solo , Óxidos , Clorofila , Ácido Pentético , Nanopartículas Metálicas/toxicidadeRESUMO
CONTEXT: Despite the important role of inflammation-related factors on the occurrence of chronic diseases, there is still conflicting evidence about the effects of the ketogenic diet (KD) on these factors. OBJECTIVE: In order to obtain a better viewpoint, this study aimed to comprehensively investigate the effects of a KD on inflammation-related markers. DATA SOURCES: To find pertinent randomized controlled trials up to August 2023, databases including PubMed/Medline, Web of Science, Scopus, Cochrane Library, and Embase were searched. DATA EXTRACTION: This study included all randomized controlled trials investigating the effects of a KD on C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-8, and IL-10 levels. Pooled weighted mean difference (WMD) and 95% confidence intervals (CIs) were achieved by random-effects model analysis for the best estimation of outcomes. DATA ANALYSIS: Forty-four studies were included in this article. The pooled findings showed that a KD has an effect on lowering TNF-α (WMD: -0.32 pg/mL; 95% CI: -0.55, -0.09; P = 0.007) and IL-6 (WMD: -0.27 pg/mL; 95% CI: -0.52, -0.02; P = 0.036) compared with control groups. However, no significant effect was reported for others inflammation marker-related levels. The results of the subgroup analysis showed that, in trials following the KD for ≤8 weeks and in people aged ≤50 years, the reduction in TNF-α levels was significantly higher than in other groups. In addition, in people with a body mass index greater than 30 kg/m2 compared to a body mass index ≤30 kg/m2, IL-6 levels decreased to a greater extent after receiving the KD. CONCLUSIONS: Consequently, adherence to a KD appears to improve some markers associated with inflammation, including TNF-α and IL-6.
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Creation of intrapenetrated mesopores with open highway from external surface into the interior of zeolite crystals are highly desirable that can significantly improve the molecular transport and active sites accessibility of microporous zeolites to afford enhanced catalytic properties. Here, different from traditional zeolite-seeded methods that generally produced isolated mesopores in zeolites, nanosized amorphous protozeolites with embryo structure of zeolites were used as seeds for the construction of single-crystalline hierarchical ZSM-5 zeolites with intrapenetrated mesopores (mesopore volume of 0.51â cm3 g-1 ) and highly complete framework. In this strategy, in contrast to the conventional synthesis, only a small amount of organic structure directing agents and a low crystallization temperature were adopted to promise the protozeolites as the dominant growth directing sites to induce crystallization. The protozeolite nanoseeds provided abundant nucleation sites for surrounding precursors to be crystallized, followed by oriented coalescence of crystallites resulting in the formation of intrapenetrated mesopores. The as-prepared hierarchical ZSM-5 zeolites exhibited ultra-long lifetime of 443.9â hours and a high propylene selectivity of 47.92 % at a WHSV of 2â h-1 in the methanol-to-propylene reaction. This work provides a facile protozeolite-seeded strategy for the synthesis of intrapenetrated hierarchical zeolites that are highly effective for catalytic applications.
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Pneumothorax is a condition involving a collapsed lung, which requires accurate segmentation of computed tomography (CT) images for effective clinical decision-making. Numerous convolutional neural network-based methods for medical image segmentation have been proposed, but they often struggle to balance model complexity with performance. To address this, we introduce the Efficient Feature Alignment Network (EFA-Net), a novel medical image segmentation network designed specifically for pneumothorax CT segmentation. EFA-Net uses EfficientNet as an encoder to extract features and a Feature Alignment (FA) module as a decoder to align features in both the spatial and channel dimensions. This design allows EFA-Net to achieve superior segmentation performance with reduced model complexity. In our dataset, our method outperforms various state-of-the-art methods in terms of accuracy and efficiency, achieving a Dice coefficient of 90.03%, an Intersection over Union (IOU) of 81.80%, and a sensitivity of 88.94%. Notably, EFA-Net has significantly lower FLOPs (1.549G) and parameters (0.432M), offering better robustness and facilitating easier deployment. Future work will explore the integration of downstream applications to enhance EFA-Net's utility for clinicians and patients in real-world diagnostic scenarios. The source code of EFA-Net is available at: https://github.com/tianjiamutangchun/EFA-Net .
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Armadilhas Extracelulares , Pneumotórax , Atelectasia Pulmonar , Humanos , Pneumotórax/diagnóstico por imagem , Tomada de Decisão Clínica , Tomografia Computadorizada por Raios XRESUMO
PURPOSE: The main treatment options of neurogenic bladder remains catheterization and long-term oral medications. Metabolic interventions have shown good therapeutic results in many diseases. To date, no studies have characterized the metabolites of the detrusor muscle during neurogenic bladder. Using metabolomics, new muscle metabolomic signatures were identified to reveal the temporal metabolic profile of muscle during disease progression. METHODS: We used 42 Sprague-Dawley rats (200±20 g, males) for T10 segmental spinal cord injury modeling and collected detrusor tissue and performed nontargeted metabolomics after sham surgery, 30-minute, 6-hour, 12-hour, 24-hour, 5-day, and 2-week postmodelling, to identify the dysregulated metabolic pathways and key metabolites. RESULTS: By comparing mzCloud, mzVault, MassList, we identified a total of 1,271 metabolites and enriched a total of 12 metabolism-related pathways with significant differences (P<0.05) based on Kyoto Encyclopedia of Genes and Genomes analysis. Metabolites in several differential metabolic pathways such as ascorbate and aldarate metabolism, Steroid hormone biosynthesis, and carbon metabolism are altered in a regular manner before and after ridge shock. CONCLUSION: Our study is the first time-based metabolomic study of rat forced urinary muscle after traumatic spinal cord injury, and we identified multiple differential metabolic pathways during injury that may improve long-term management strategies for neurogenic bladder and reduce costs in long-term treatment.
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The generation of neurons from quiescent radial-glia-like neural stem cells (RGLs) in adult brain goes hand in hand with the modulation of cellular metabolism. However, it is still unclear how the exact metabolic program governs the balance between quiescent and activated RGLs. Here, we find that loss of mitochondrial D-2-hydroxyglutarate dehydrogenase (D2HGDH) leads to aberrant accumulation of D-2-hydroxyglutarate (D-2-HG) and impaired RGL activation. Mechanistically, accumulated D-2-HG bonds directly to ATP-citrate lyase and competitively inhibits its enzymatic activity, thereby reducing acetyl-CoA production and diminishing histone acetylation. However, administration of acetate restores the acetyl-CoA levels via acetyl-CoA synthetase-mediated catabolism and rescues the deficiencies in histone acetylation and RGL activation caused by loss of D2HGDH. Therefore, our findings define the role of cross talk between mitochondria and the nucleus via a mitochondrial metabolite, D-2-HG, the aberrant accumulation of which hinders the regulation of histone acetylation in RGL activation and attenuates continuous neurogenesis in adult mammalian brain.
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ATP Citrato (pro-S)-Liase , Células-Tronco Neurais , Animais , ATP Citrato (pro-S)-Liase/metabolismo , Histonas/metabolismo , Acetilcoenzima A/metabolismo , Acetilação , Células-Tronco Neurais/metabolismo , Trifosfato de Adenosina/metabolismo , Mamíferos/metabolismoRESUMO
Zeolites are widely used as catalysts and adsorbents in the chemical industry, but their potential for electronic devices has been stunted to date, as they are commonly recognized as electronic insulators. Here, we have for the first time demonstrated that Na-type ZSM-5 zeolites are ultrawide-direct-band-gap semiconductors based on optical spectroscopy, variable-temperature current-voltage characteristics, and photoelectric effect as well as electronic structure theoretical calculations and further unraveled the band-like charge transport mechanism in electrically conductive zeolites. The increase in charge-compensating Na+ cations in Na-ZSM-5 decreases the band gap and affects its density of states, shifting the Fermi level close to the conduction band. Remarkably, the semiconducting Na-ZSM-5 zeolites have been first applied for constructing electrically transduced sensors that can sense trace-level (77 ppb) ammonia with unprecedentedly high sensitivity, negligible cross-sensitivity, and high stability under moisture ambient conditions compared with conventional semiconducting materials and conductive metal-organic frameworks (MOFs). The charge density difference shows that the massive electron transfer between NH3 molecules and Na+ cations ascribed to Lewis acid sites enables electrically transduced chemical sensing. This work opens a new era of zeolites in applications of sensing, optics, and electronics.
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This study investigated the impact of endogenous sodium and potassium ions in plants on the quality of alfalfa silage, as well as the stability of bacterial communities during fermentation. Silage was produced from the fermented alfalfa, and the chemical composition, fermentation characteristics, and microbiome were analyzed to understand their interplay and impact on silage fermentation quality. The alfalfa was cultivated under salt stress with the following: (a) soil content of <1 (CK); (b) 1-2 (LP); (c) 2-3 (MP); (d) 3-4 (HP). The results revealed that the pH of silage was negatively correlated with the lactic acid content. With the increase of lactic acid (LA) content increased (26.3-51.0 g/kg DM), the pH value decreased (4.9-5.3). With the increase of salt stress, the content of Na+ in silage increased (2.2-5.4 g/kg DM). The presence of endogenous Na+ and K+ ions in plants significantly affected the quality of alfalfa silage and the dynamics of bacterial communities during fermentation. Increased salt stress led to changes in microbial composition, with Lactococcus and Pantoea showing a gradual increase in abundance, especially under high salt stress. Low pH inhibited the growth of certain bacterial genera, such as Pantoea and Pediococcus. The abundance of Escherichia-Shigella and Comamonas negatively correlated with crude protein (CP) content, while Enterococcus and Lactococcus exhibited a positive correlation. Furthermore, the accumulation of endogenous Na+ in alfalfa under salt stress suppressed bacterial proliferation, thereby reducing protein degradation during fermentation. The pH of the silage was high, and the LA content was also high. Silages from alfalfa under higher salt stress had higher Na+ content. The alpha diversity of bacterial communities in alfalfa silages showed distinct patterns. Desirable genera like Lactococcus and Lactobacillus predominated in silages produced from alfalfa under salt stress, resulting in better fermentation quality.
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High-quality alfalfa is an indispensable resource for animal husbandry and sustainable development. Its nutritional quality changes dramatically during its life cycle and, at present, no molecular mechanisms for nutrient metabolic variation in alfalfa leaves at different growth stages have been clearly reported. We have used correlation and network analyses of the alfalfa leaf metabolome, proteome, and transcriptome to explore chlorophyll, flavonoid, and amino acid content at two development stages: budding stage (BS) and full-bloom stage (FBS). A high correlation between the expression of biosynthetic genes and their metabolites revealed significant reductions in metabolite content as the plant matured from BS to FBS. l-Glutamate, the first molecule of chlorophyll biosynthesis, decreased, and the expression of HemA, which controls the transformation of glutamyl-tRNA to glutamate 1-semialdehyde, was down-regulated, leading to a reduction in leaf chlorophyll content. Flavonoids also decreased, driven at least in part by increased expression of the gene encoding CYP75B1: flavonoid 3'-monooxygenase, which catalyzes the hydroxylation of dihydroflavonols and flavonols, resulting in degradation of flavonoids. Expression of NITRILASE 2 (NIT2) and Methyltransferase B (metB), which regulate amino acid metabolism and influence the expression of genes of the glycolysis-TCA pathway, were down-regulated, causing amino acid content in alfalfa leaves to decrease at FBS. This study provides new insights into the complex regulatory network governing the content and decrease of chlorophyll, amino acids, flavonoids, and other nutrients in alfalfa leaves during maturation. These results further provide a theoretical basis for the generation of alfalfa varieties exhibiting higher nutritional quality, high-yield cultivation, and a timely harvest.
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PURPOSE: This study aimed to develop a minimally invasive surgical procedure for laminar lift and posterior cervical laminoplasty via the intermuscular approach using a canine model. METHODS: Six Alaskan dogs were used for developing the surgical approach. The bilateral laminae of C3-7 were cut with an ultrasonic osteotome and fixed with bilateral plates to maintain the lamina lifting and reshape a wider spinal canal. The important structures, such as ligaments, supraspinous ligaments, interspinous ligaments, and ligamentum flavum were preserved. The therapeutic effect was evaluated by preoperative and postoperative imaging results and neck mobility. RESULTS: The surgical procedures were all successfully performed in the 6 animals. All the dogs survived well within 1 year of postoperative follow-up. The postoperative neck mobility was as good as the preoperative one. Computed tomography results showed that the anteroposterior diameter of the spinal canal was successfully enlarged and maintained well. CONCLUSIONS: The minimally invasive surgical procedure for laminar lift and posterior cervical laminoplasty via the intermuscular approach was feasible in a canine model, which might be applied in clinical practice.
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Laminoplastia , Cães , Animais , Humanos , Ligamentos , Pescoço , Período Pós-Operatório , Tomografia Computadorizada por Raios XRESUMO
Subculture, includes animation, comics, games (ACG), and idol fan culture, is popular among young generations in Japan. Previous studies have shown different psychological outcomes within different subcultural groups; however, underlying mechanisms remain unclear. This study proposes that subcultural identity may play a crucial role in mental health outcomes by interweaving social support and reputation. We examined the interplay between subcultural identity, social support, reputation, and different psychological outcomes through structural equation modeling (N = 300). Furthermore, we trained neural networks (NN) by applying a deep learning algorithm to predict psychological outcomes of different subcultures. The results suggest identity related to anime, idol, and hip-hop was positively associated with anxiety, aggression, depressive symptoms, and suicidal tendencies. By contrast, fashion and sports identities associated with no adverse or positive psychological outcomes. Perceived reputation mediates adverse psychological outcomes in the ACG, idol, and hip-hop groups. The highest accuracy in our NN reached 71%, indicating that NN could be an effective tool for predicting mental problems. Our work points up an urgent need to improve the mental health of the young generation by de-stigmatizing discriminated cultural groups.
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Hierarchical zeolites integrating intrinsic micropores with secondary meso- and/or macropores afford superior catalytic properties for enhanced mass transportation and more accessible active sites. The mesopores generated by using mesoporogens or framework etching are usually irregular with abundant defective sites and low hydrothermal stability. Here, using protozeolite nanoparticles as seeds, we succeeded in the synthesis of single-crystalline hierarchical ZSM-5 zeolites with hexagonal mesopores faceted by complete microporous frameworks. The protozeolites played a key role in the formation of faceted mesopores achieved via intraparticle ripening process. Thanks to the unique texture properties and more accessible acid sites at the open location, the as-prepared zeolites exhibited remarkedly long lifetime (18â h) and high propylene selectivity (52.7 %) with propylene/ethylene ratio of 3.64 in the methanol-to-propylene reaction (WHSV=12.5â h-1 ) compared to the counterparts.
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Nano copper oxide (CuO NP) was added to eight soils to study the effect of aging time of copper on the concentration of diethylenetriaminepentaacetic acid (DTPA)-extracted copper (DTPA-Cu), with bulk copper oxide (CuO BP) and copper nitrate [Cu(NO3)2] used for comparison. Moreover, the effect of soil properties on the dissolution of CuO NP was studied. A dissolution model was used to quantitatively describe the dissolution kinetics of CuO NPs in different soils. The results showed that the concentration of DTPA-Cu decreased with increasing aging time in soils spiked with Cu(NO3)2, while the concentration increased to varying degrees in soils spiked with CuO NPs or CuO BPs. In acidic soils, the equilibrium concentrations of DTPA-Cu were 93.3-98.7 mg·kg-1 for CuO NP treatments, 65.5-94.3 mg·kg-1 for CuO BP treatments, and 81.4-90.0 mg·kg-1 for Cu(NO3)2 treatments, which were greater than those in alkaline soils (43.4-56.9 mg·kg-1, 6.26-8.61 mg·kg-1, and 73.9-80.0 mg·kg-1, respectively). In acidic soils, DTPA-Cu equilibrium concentration ranked the different forms of copper treatments as CuO NPs > Cu(NO3)2 > CuO BPs, while in alkaline soils, the order was Cu(NO3)2 > CuO NPs > CuO BPs. The dissolution rate constants and solubility of CuO NPs were 0.33-6.42 and 37.1-100.1 mg·kg-1, respectively. Pearson correlation analysis indicated that the dissolution parameters of CuO NPs were negatively correlated with soil pH and positively correlated with the contents of organic matter, clay, iron oxides, and aluminum oxides. Further, the dissolution rate constant and solubility of CuO NPs could be well predicted by soil pH and the content of free or amorphous aluminum. Our study identified the main factors controlling the dissolution of CuO NPs in farmland soils and highlighted the higher availability of CuO NPs in acidic soils.
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Nanopartículas Metálicas , Nanopartículas , Alumínio/análise , Cobre/química , Cinética , Óxidos/análise , Ácido Pentético , Solo , SolubilidadeRESUMO
Trace elements like copper and zinc are supplemented in swine feeds to suppress bacteria and/or promote growth. Due to low absorption rates, trace elements are highly concentrated in swine feces, posing a risk to soil and human health if applied to agricultural fields. In the present study, the concentrations of six trace elements (Cu, Zn, As, Cd, Pb, and Cr) in pig feed and feces were evaluated by sample analysis and data from the literature. The feed-to-feces and feces-to-compost enrichment factors of the trace elements were determined to back-calculate safe concentrations in feed needed to meet permissible trace element concentrations in organic fertilizers. The Cu and Zn concentrations in feeds were the highest, being statistically significantly higher in starter pig feeds than in grower-finisher feeds. Copper exceeded feed limits mainly in the feeds of finisher pigs and sows, while exceedance for Zn occurred in almost all feed samples. Concentrations above the limits were also observed for Pb and Cr impurities in mineral feed supplements. The highest Cu and Zn concentrations in feces were observed for starter pigs. Moreover, significant enrichment of trace elements from feeds to feces was found, with enrichment factors calculated as: 4.68-6.11 for Cu, 3.43-4.60 for Zn, 2.30-3.12 for As, 2.89-4.63 for Cd, 2.45-5.00 for Pb, and 3.32-5.00 for Cr. On this basis, the recommended calculated limits for Cu, Zn, As, Cd, Pb, and Cr in feeds for different ages of pigs were 41-53, 130-175, 2-3, 0.3-0.5, 5-10, and 15-23 mg/kg, respectively. Priority index calculations and the calculated recommended limits indicated that Cu in starter pig feeds and Cd in starter and breeding pig feeds should be prioritized for reduction to enable feces compost to be utilized safely in agricultural land amendment. Integr Environ Assess Manag 2022;18:978-987. © 2021 SETAC.
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Metais Pesados , Oligoelementos , Ração Animal/análise , Animais , Cádmio/análise , China , Cobre/análise , Fazendas , Fezes/química , Feminino , Chumbo/análise , Metais Pesados/análise , Suínos , Oligoelementos/análiseRESUMO
Purpose: This study aimed to develop a minimally invasive surgical procedure for laminar lift and posterior cervical laminoplasty via the intermuscular approach using a canine model. Methods: Six Alaskan dogs were used for developing the surgical approach. The bilateral laminae of C3-7 were cut with an ultrasonic osteotome and fixed with bilateral plates to maintain the lamina lifting and reshape a wider spinal canal. The important structures, such as ligaments, supraspinous ligaments, interspinous ligaments, and ligamentum flavum were preserved. The therapeutic effect was evaluated by preoperative and postoperative imaging results and neck mobility. Results: The surgical procedures were all successfully performed in the 6 animals. All the dogs survived well within 1 year of postoperative follow-up. The postoperative neck mobility was as good as the preoperative one. Computed tomography results showed that the anteroposterior diameter of the spinal canal was successfully enlarged and maintained well. Conclusions: The minimally invasive surgical procedure for laminar lift and posterior cervical laminoplasty via the intermuscular approach was feasible in a canine model, which might be applied in clinical practice.