RESUMO
NaoShuanTong capsule (NSTC), an oral traditional Chinese medicine formula, is composed of Pollen Typhae, Radix Paeoniae Rubra, Rhizoma Gastrodiae, Radix Rhapontici and Radix Curcumae. It has been widely used to treat ischemic stroke in clinic for many years in China. In addition to neuronal apoptosis, haemorheology and cerebral energy metabolism disorders also play an important role in the pathogenesis and development of ischemic stroke. The present study was designed to evaluate the in vivo protective effects of NSTC on haemorheology and cerebral energy metabolism disorders in rats with blood stasis. Sixty specific pathogen-free sprague-dawley rats, male only, were randomly divided into six groups (control group, model group, aspirin (100 mg/kg/d) group, NSTC low-dose (400 mg/kg/d) group, NSTC intermediate-dose (800 mg/kg/d) group, NSTC high-dose (1600 mg/kg/d) group) with 10 animals in each. The rats except those in the control group were placed in ice-cold water (0-4 °C) for 5 min during the time interval (4 h) of two adrenaline hydrochloride injections (0.8 mg/kg) to induce blood stasis. After treatment, whole blood viscosity at three shear rates, plasma viscosity and erythrocyte sedimentation rate significantly decreased in NSTC intermediate- and high-dose groups; erythrocyte aggregation index and red corpuscle electrophoresis index significantly decreased in all the three dose NSTC groups. Moreover, treatment with high-dose NSTC could significantly improve Na+-K+ adenosine triphosphatase (ATPase) and Ca2+ ATPase activity, as well as lower lactic acid level in brain tissues. These results demonstrated the protective effects of NSTC on haemorheology and cerebral energy metabolism disorders, which may provide scientific information for the further understanding of mechanism(s) of NSTC as a clinical treatment for ischemic stroke. Furthermore, the protective effects of activating blood circulation as observed in this study might create valuable insight for the utilisation of NSTC to be a feasible alternative therapeutic agent for patients with blood stasis.
RESUMO
OBJECTIVE: To establish the HPLC fingerprint of Compound Xueshuantong Capsule. METHODS: Dionex Acclaim 120 C18 column (4.6 mm x 150 mm, 3 microm) was used with acetonitrile (A) and 0.05% phosphoric acid (B) in gradient elution mode. The elution profile was:0-50 min (15%-->34% A), 50-95 min (34%-->75% A); The detective wavelength was 203 nm and 270 nm. The column temperature was set at 25 degrees C and the flow rate was 1.0 mL/min. RESULTS: In the established fingerprint,42 common peaks covering 4 medicinal materials were detected, and 23 chemical compounds were identified by RRLC/MS/MS and DAD spetra. CONCLUSION: The method can be used for quality control of Compound Xueshuantong Capsule with great precision, accuracy and good reproducibility.