RESUMO
The paper presents the results of studying the effect of the antiviral drug Ingavirin on different stages of intracellular transformations of influenza A virus nucleocapsid protein (NP). Ingavirin 400-1000 microg/ml has been found to impair the biogenesis of influenza virus NP, to lower the efficiency of formation of conformationally mature compact NP oligomers, and to retard the migration of newly-synthesized NP from the cytoplasm to the nucleus. It is shown that there is an association of tritium-labeled Ingavirin with the nuclear membranes of MDCK cells. The investigations of the mechanisms of antiviral activity of Ingavirin are not only important for the characterization of this drug, but also promote the detection of potential targets to design novel antiviral agents.
Assuntos
Amidas/farmacologia , Antivirais/farmacologia , Ácidos Dicarboxílicos/farmacologia , Imidazóis/farmacologia , Vírus da Influenza A Subtipo H3N8/fisiologia , Proteínas do Nucleocapsídeo/metabolismo , Infecções por Orthomyxoviridae/virologia , Animais , Caproatos , Linhagem Celular , Núcleo Celular/metabolismo , Citoplasma/metabolismo , Cães , Vírus da Influenza A Subtipo H3N8/química , Vírus da Influenza A Subtipo H3N8/efeitos dos fármacos , Proteínas do Nucleocapsídeo/efeitos dos fármacos , Transporte Proteico/efeitos dos fármacos , Montagem de Vírus/efeitos dos fármacosRESUMO
The effect of the antiviral drug arbidol on the reproduction of avian influenza A/H5 viruses was studied in in vitro experiments. The strains were isolated from the wild birds of Eastern Siberia and they were closely related to the 1997-2000 viruses from South-Eastern Asia. Arbidol was shown to exert a selective inhibiting effect on the reproduction of these viruses in the MDCH cell cultures.