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1.
J Biomed Mater Res A ; 100(10): 2732-8, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22623404

RESUMO

More than 400,000 primary hip and knee replacement surgeries are performed each year in the United States. From these procedures, approximately 0.5-3% will become infected and when considering revision surgeries, this rate has been found to increase significantly. Antibiotic-resistant bacterial infections are a growing problem in patient care. This in vitro research investigated the antimicrobial potential of the polymer released, broad spectrum, Cationic Steroidal Antimicrobial-13 (CSA-13) for challenges against 5 × 10(8) colony forming units (CFU) of methicillin-resistant Staphylococcus aureus (MRSA). It was hypothesized that a weight-to-weight (w/w) concentration of 18% CSA-13 in silicone would exhibit potent bactericidal potential when used as a controlled release device coating. When incorporated into a polymeric device coating, the 18% (w/w) broad-spectrum polymer released CSA-13 antimicrobial eliminated 5 × 10(8) CFU of MRSA within 8 h. In the future, these results will be utilized to develop a sheep model to assess CSA-13 for the prevention of perioperative device-related infections in vivo.


Assuntos
Anti-Infecciosos/uso terapêutico , Materiais Revestidos Biocompatíveis/farmacologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Polímeros/química , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/prevenção & controle , Anti-Infecciosos/farmacologia , Contagem de Colônia Microbiana , Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Porosidade , Infecções Estafilocócicas/microbiologia , Esteroides/farmacologia , Esteroides/uso terapêutico , Fatores de Tempo
2.
J Clin Microbiol ; 45(12): 3979-85, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17928419

RESUMO

In 1994, an outbreak of Enterobacter sakazakii infections occurred in a neonatal intensive care unit in France from 5 May to 11 July. During the outbreak, 13 neonates were infected with E. sakazakii, resulting in 3 deaths. In addition, four symptomless neonates were colonized by E. sakazakii. The strains were subjected to 16S rRNA gene sequence analysis, genotyped using pulsed-field gel electrophoresis, and phenotyped for a range of enzyme activities. E. sakazakii was isolated from various anatomical sites, reconstituted formula, and an unopened can of powdered infant formula. A fourth neonate died from septic shock, attributed to E. sakazakii infection, during this period. However, 16S rRNA gene sequence analysis revealed that the organism was Enterobacter cloacae. There were three pulsotypes of E. sakazakii associated with infected neonates, and three neonates were infected by more than one genotype. One genotype matched isolates from unused prepared formula and unfinished formula. However, no pulsotypes matched the E. sakazakii strain recovered from an unopened can of powdered infant formula. One pulsotype was associated with the three fatal cases, and two of these isolates had extended-spectrum beta-lactamase activity. It is possible that E. sakazakii strains differ in their pathogenicities, as shown by the range of symptoms associated with each pulsotype.


Assuntos
Cronobacter sakazakii/classificação , Cronobacter sakazakii/isolamento & purificação , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Surtos de Doenças , Infecções por Enterobacteriaceae/epidemiologia , Infecções por Enterobacteriaceae/microbiologia , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , Cronobacter sakazakii/genética , Cronobacter sakazakii/fisiologia , Infecção Hospitalar/mortalidade , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Eletroforese em Gel de Campo Pulsado , Infecções por Enterobacteriaceae/mortalidade , Feminino , França , Genes de RNAr , Genótipo , Humanos , Lactente , Alimentos Infantis/microbiologia , Terapia Intensiva Neonatal , Masculino , Dados de Sequência Molecular , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , beta-Lactamases/biossíntese
3.
Appl Environ Microbiol ; 71(11): 6554-63, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16269681

RESUMO

Colonization of broiler chickens by the enteric pathogen Campylobacter jejuni is widespread and difficult to prevent. Bacteriophage therapy is one possible means by which this colonization could be controlled, thus limiting the entry of campylobacters into the human food chain. Prior to evaluating the efficacy of phage therapy, experimental models of Campylobacter colonization of broiler chickens were established by using low-passage C. jejuni isolates HPC5 and GIIC8 from United Kingdom broiler flocks. The screening of 53 lytic bacteriophage isolates against a panel of 50 Campylobacter isolates from broiler chickens and 80 strains isolated after human infection identified two phage candidates with broad host lysis. These phages, CP8 and CP34, were orally administered in antacid suspension, at different dosages, to 25-day-old broiler chickens experimentally colonized with the C. jejuni broiler isolates. Phage treatment of C. jejuni-colonized birds resulted in Campylobacter counts falling between 0.5 and 5 log10 CFU/g of cecal contents compared to untreated controls over a 5-day period postadministration. These reductions were dependent on the phage-Campylobacter combination, the dose of phage applied, and the time elapsed after administration. Campylobacters resistant to bacteriophage infection were recovered from phage-treated chickens at a frequency of <4%. These resistant types were compromised in their ability to colonize experimental chickens and rapidly reverted to a phage-sensitive phenotype in vivo. The selection of appropriate phage and their dose optimization are key elements for the success of phage therapy to reduce campylobacters in broiler chickens.


Assuntos
Bacteriófagos/fisiologia , Infecções por Campylobacter/veterinária , Campylobacter jejuni/virologia , Galinhas , Doenças das Aves Domésticas/prevenção & controle , Animais , Bacteriófagos/crescimento & desenvolvimento , Infecções por Campylobacter/microbiologia , Infecções por Campylobacter/prevenção & controle , Infecções por Campylobacter/terapia , Campylobacter jejuni/crescimento & desenvolvimento , Campylobacter jejuni/patogenicidade , Ceco/microbiologia , Contagem de Colônia Microbiana , Intestinos/microbiologia , Masculino , Doenças das Aves Domésticas/microbiologia , Doenças das Aves Domésticas/terapia
4.
Appl Environ Microbiol ; 70(7): 3877-83, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15240258

RESUMO

A longitudinal study of bacteriophages and their hosts was carried out at a broiler house that had been identified as having a population of Campylobacter-specific bacteriophages. Cloacal and excreta samples were collected from three successive broiler flocks reared in the same barn. Campylobacter jejuni was isolated from each flock, whereas bacteriophages could be isolated from flocks 1 and 2 but were not isolated from flock 3. The bacteriophages isolated from flocks 1 and 2 were closely related to each other in terms of host range, morphology, genome size, and genetic content. All Campylobacter isolates from flock 1 were genotypically indistinguishable by pulsed-field gel electrophoresis (PFGE). PFGE and multilocus sequence typing indicated that this C. jejuni type was maintained from flock 1 to flock 2 but was largely superseded by three genetically distinct C. jejuni types insensitive to the resident bacteriophages. All isolates from the third batch of birds were insensitive to bacteriophages and genotypically distinct. These results are significant because this is the first study of an environmental population of C. jejuni bacteriophages and their influence on the Campylobacter populations of broiler house chickens. The role of developing bacteriophage resistance was investigated as this is a possible obstacle to the use of bacteriophage therapy to reduce the numbers of campylobacters in chickens. In this broiler house succession was largely due to incursion of new genotypes rather than to de novo development of resistance.


Assuntos
Bacteriófagos/isolamento & purificação , Campylobacter jejuni/isolamento & purificação , Campylobacter jejuni/virologia , Galinhas/microbiologia , Galinhas/virologia , Animais , Bacteriófagos/genética
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