RESUMO
CONTEXT: 4-Iodo-2,5-dimethoxy-N-(2-methoxybenzyl)phenethylamine (25I-NBOMe) is a N-methoxybenzyl-substituted phenethylamine with potent serotoninergic effects. We describe seven cases of analytically confirmed toxicity due to the recreational use of 25I-NBOMe in the United Kingdom. CASE SERIES: Seven patients, all young adult males, presented to hospitals in the northeast of England with clinical toxicity after recreational drug use in January 2013. Clinical features included tachycardia (n = 7), hypertension (4), agitation (6), aggression, visual and auditory hallucinations (6), seizures (3), hyperpyrexia (3), clonus (2), elevated white cell count (2), elevated creatine kinase (7), metabolic acidosis (3), and acute kidney injury (1). LC-MS/MS analysis identified 25I-NBOMe as the main active substance in the plasma of all seven cases. CONCLUSIONS: Severe clinical toxicity may occur following recreational use of 25I-NBOMe, with stimulant and serotoninergic features predominating. Clinicians should be alert to this substance, in view of its emergence in Europe as well as in the United States.
Assuntos
Benzilaminas/toxicidade , Drogas Ilícitas/toxicidade , Fenetilaminas/toxicidade , Adulto , Acatisia Induzida por Medicamentos/etiologia , Benzilaminas/sangue , Dimetoxifeniletilamina/análogos & derivados , Inglaterra/epidemiologia , Cromatografia Gasosa-Espectrometria de Massas , Alucinações/induzido quimicamente , Humanos , Hipertensão/induzido quimicamente , Drogas Ilícitas/sangue , Masculino , Fenetilaminas/sangue , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Taquicardia/induzido quimicamente , Adulto JovemRESUMO
Traditional ex vivo culture setups fail to imitate the native tissue niche, leading to cellular senescence, phenotypic drift, growth arrest and loss of stem cell multipotency. Growing evidence suggests that surface topography, substrate stiffness, mechanical stimulation, oxygen tension and localised density influence cellular functions and longevity, enhance tissue-specific extracellular matrix deposition and direct stem cell differentiation. In this review, we discuss how these cues will facilitate engineering of physiological in vitro microenvironments to enable clinical translation of cell based therapies and development of in vitro models for drug discovery applications.