[Preparation of novel chiral stationary phases for ligand exchange chromatography and their applications].

Two novel chiral stationary phases for ligand exchange chromatography were prepared by synthesizing 2-(2-hydroxyl-3-octoxy)propyl-(S)-1, 2, 3, 4-tetrahydro-3-isoquinolinecarboxylic acid (C8-(S)-THIQCA) and 2-(2-hydroxyl-3-dodecoxy)propyl-(S)-1, 2, 3, 4-tetrahydro-3-isoquinolinecarboxylic acid (C12-(S)-THIQCA) as chiral selectors, and coating them on ODS bonded stationary phase. Chromatographic resolutions of some DL-amino acids were achieved on the chiral stationary phases by using 1 mmol/L NH4Cl with 0.2 mmol/L Cu(Ac)2 solution as mobile phase and detection at 254 nm. Ability of chiral resolution of the DL-amino acids through the stationary phases was compared and the effect of cupric ion on resolutions of the DL-amino acids was examined. The elution order of D-isomer before L-isomer was observed for all the DL-amino acids resolved, except DL-Val. For DL-Pro, DL-Val and DL-Leu the elution order through the chiral phases was different from that through the chiral ligand exchange phases prepared from L-Pro or L-hydroxyl proline with five membered ring structure.

[Protective action of sophoridine on acute myocardial infarction in anesthetized dogs].

The effects of sophoridine on acute myocardial infarction induced by ligation of left anterior descending coronary artery in 16 anesthetized and open-chest dogs were studied. Treatment with sophoridine 20 mg.kg-1 iv reduced the size of myocardial infarct 6 h after ligation and lowered the elevated plasma levels of lactic acid and pyruvic acid 15, 30 and 60 min after ligation. Sophoridine also lowered the activity of serum CPK 60 and 120 min after ligation and reduced TXB2 and increased 6-keto-PGF1 alpha, hence the metabolites of PGI2/TXA2 ratio after ligation was increased. Electron microscopy showed that sophoridine protected mitochondria of ischemic cells. These results indicated that sophoridine showed protective effects on acute myocardial infarction. The beneficial effect on PGI2/TXA2 and mitochondria seems to contribute its protective action.