Full-length transcriptome analysis of Misgurnus anguillicaudatus.

Faithful analysis of transcripts represents a critical component of the gene discovery, genomic characterization and annotation process in species without a reference genome. The present study generated 28,001 full-length transcripts of Misgurnus anguillicaudatus using the Pacific Bioscience (PacBio) single-molecule real-time (SMRT) sequencing system. A total of 77,346 consensus isoforms were identified, and 18,991 complete open reading frames (ORFs) detected. In total, 1345 lncRNAs were identified with high-confidence, with additional identification of a number of well-known transcription factors. The present study may facilitate additional exploration of the genomic signatures of M. anguillicaudatus.

Effects of cadmium pollution on the safety of rice and fish in a rice-fish coculture system.

The rice-fish coculture system (RFS) is one of the most important and environmentally friendly agricultural systems in the world. With the increasing amounts of heavy metal contamination in the soil and water, the safe production of RFS has been greatly threatened. However, there are no reports on heavy metal uptake by rice and fish in a RFS. In this study, a model of cadmium (Cd)-contaminated RFS with the addition of 0-40.00 mg kg-1Cd was simulated in the field. The accumulation of Cd in the rice and fish increased as the level of Cd contamination increased. Regardless of the level of contamination, the order of Cd accumulation in the rice was root > stem ≈ leaf > rice grain > brown grain and in the fish was liver ≈ gut > kidney > gill > muscle. The dissolved oxygen (DO) and the transparency of water were significantly reduced after the fish were added. The tendency of the Cd to accumulate in the fish correlated with the change of the concentration of Cd in the water (P < 0.05). According to the maximum level of Cd in the brown grains (0.40 mg kg-1) and in the fish muscle (0.10 mg kg-1) of Codex Alimentarius Commission (CAC), the safety threshold of soil Cd for the rice and the fish was calculated to be 5.86 mg kg-1 and 31.47 mg kg-1, respectively, indicating that the safety risk to the rice was much greater in a Cd-contaminated RFS.