Nanoconjugates based on a novel organic-inorganic hybrid silsesquioxane and gold nanoparticles as hemocompatible nanomaterials for promising biosensing applications.

A new hybrid organic-inorganic silsesquioxane material, 3-n-propyl(2-amino-4-methyl)pyridium chloride (SiAMPy+Cl-), was synthesized and successfully applied for the synthesis of stable nanoconjugates with gold nanoparticles (AuNPs-SiAMPy+). SiAMPy+Cl- was obtained through a simple sol-gel procedure by using chloropropyltrimetoxysilane and tetraethylorthosilicate as precursors and 2-amino-4-methylpyridine as the functionalizing agent. The resulting material was characterized by employing FTIR, XRD, and 1H-, 13C-, and 29Si-NMR spectroscopy. The synthesis of AuNPs-SiAMPy+ nanoconjugates was optimized through a 23 full factorial design. UV-VIS, FTIR, TEM, DLS, and ζ-potential measurements were used to characterize the nanoconjugates, which presented a spherical morphology with an average diameter of 5.8 nm. To investigate the existence of toxic effects of AuNPs-SiAMPy+ on blood cells, which is essential for their future biomedical applications, toxicity assays on human erythrocytes and leukocytes were performed. Interestingly, no cytotoxic effects were observed for both types of cells. The nanoconjugates were further applied in the construction of electrochemical immunosensing devices, aiming the detection of anti-Trypanosoma cruzi antibodies in serum as biomarkers of Chagas disease. The AuNPs-SiAMPy+ significantly enhanced the sensitivity of the biodevice, which was able to discriminate between anti-T. cruzi positive and negative serum samples. Thus, the AuNPs-SiAMPy+-based biosensor showed great potential to be used as a new tool to perform fast and accurate diagnosis of Chagas disease. The promising findings described herein strongly confirm the remarkable potential of SiAMPy+Cl- to obtain nanomaterials, which can present notable biomedical properties and applications.

Pharma-molecule Transport across Bacterial Membranes: Detection and Quantification Approaches by Electrochemistry and Bioanalytical Methods.

Antibiotic resistance is a significant challenge encountered by healthcare systems on a global scale. Knowledge about membrane transport of antibiotics and other pharmacologically relevant molecules in bacteria is crucial towards understanding and overcoming antibiotic resistance, as drug resistance often depends on drug transport. This comprehensive literature review discusses the detection and quantification of membrane transport of pharma-molecules in bacteria and highlights the importance of molecule transport to antibiotic resistance. This review emphasizes electrochemical and electrophysiological methods of detection and quantification. The results of this literature review reveal a substantial diversity in methods and types of quantitative information collected.

Gold nanoparticles capped with polysaccharides extracted from pineapple gum: Evaluation of their hemocompatibility and electrochemical sensing properties.

In the present work, gold nanoparticles were synthesized through a green route by using, for the first time, polysaccharides extracted from pineapple gum (PG) as the reducing and capping agent. The obtained nanoparticles (AuNPs-PG) were characterized by UV-VIS, FTIR, TEM, FESEM, EDX, XRD, and zeta potential measurements, which confirmed that PG was effective to produce AuNPs with an average diameter of 10.3 ± 1.6 nm. The AuNPs-PG were employed as the modifier of glassy carbon paste electrodes (CPE/AuNPs-PG), which were applied as sensitive electrochemical sensors to the determination of the antihistamine drug promethazine hydrochloride (PMZ). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements showed that the AuNPs-PG could enhance the electronic transfer properties of the glassy carbon paste, which was due to their large surface area and high electrical conductivity. After optimization of the instrumental parameters of square wave voltammetry (SWV) through a Box-Behnken factorial design, a linear relationship between the anodic peak current and PMZ concentration was obtained in the range from 2.0 to 15.7 µmol L-1 in McIlvaine buffer solution pH 5.0. The detection and quantification limits were found to be equal to 1.33 and 4.44 µmol L-1, respectively. The developed sensors could successfully quantify PMZ in different commercial pharmaceutical formulations, with satisfactory levels of accuracy and precision. In addition to improving the analytical features of the electrodes, hemocompatibility assays carried out on erythrocytes and leukocytes showed that the AuNPs-PG do not exhibit toxic effects on the referred cells. This interesting behavior enables their use in biocompatible electrochemical sensing platforms as well as for future biomedical investigations.

Porphyran-capped silver nanoparticles as a promising antibacterial agent and electrode modifier for 5-fluorouracil electroanalysis.

In the present work, the green synthesis of silver nanoparticles (AgNPs) using the sulfated polysaccharide porphyran (PFR) as capping agent and d-glucose as reducing agent is described. PFR was extracted from red seaweed and characterized by employing 13C NMR and determination of total sugar, protein, and sulfate contents. The obtained AgNPs-PFR were characterized by using UV-VIS spectroscopy, zeta potential determination, FESEM, and TEM, which demonstrated that PFR was effective at capping the AgNPs, yielding stable suspensions. The AgNPs-PFR presented good antimicrobial properties against Gram-positive and Gram-negative bacterial strains (Staphylococcus aureus and Escherichia coli, respectively). The AgNPs-PFR were also employed as the modifier of carbon paste electrodes, which were efficiently applied as electrochemical sensors for the determination of 5-fluorouracil (5-FU), an important anticancer drug, through square wave voltammetry (SWV). The AgNPs-PFR improved the electrochemical properties of the electrodes, and enhanced their electroanalytical performance. The developed sensing device presented detection and quantification limits equal to 10.7 and 35.8 µmol L-1, respectively, towards 5-FU determination. The proposed electrochemical sensor successfully quantified 5-FU in a real pharmaceutical formulation, confirming its potential as a new promising analytical detection tool for 5-FU quality control purposes.