Efficacy of WaveOne Gold and ProDesign RT systems in removing filling material: a micro-CT analysis.

The remaining filling material after retreatment can harbor bacteria and organic tissues that can influence the outcome of the therapy. AIM: The aim of this study was to evalúate, by micro-CT, the amount of filling material remaining in the root canal after its removal using WaveOne Gold or ProDesign RT. MATERIAL AND METHOD: Forty human mandibular canines were instrumented with the ProTaper Next system up to the X2 instrument (25.06) and filled with gutta-percha cones and AHPlus. Teeth were divided into 2 groups (n=20): WaveOne Gold 25.07 (WOG) and ProDesign RT 25.08 (PRT) for filling removal, after which they were scanned in a micro-CT device to quantify the volume of remaining filling material. The data were subjected to log 10 transformation, Student 's t-test was performed to account for multiple observationsper sample, significance was set at 5%. RESULTS: Student 's t-test showed that there was no difference between the two systems regarding the volume of remaining filling material in the thirds: apical (p = 0.392), middle (p = 0.065), or cervical (p = 0.918). CONCLUSIÓN: Remaining filling material was present in all groups and both systems were similar in removing root filling material in mandibular canines.

A permanencia de material obturador após o retratamento pode abrigar bactérias e tecidos orgánicos que podem influenciar o resultado da terapia. OBJETIVO: O objetivo deste estudo foi avaliar, por micro-CT, a quantidade de material obturador remanescente no canal radicular após a desobturagdo com WaveOne Gold e ProDesign RT. MATERIAL E MÉTODO: Quarenta caninos inferiores humanos foram instrumentados com o sistema ProTaper Next até o instrumento X2 (25.06) e obturados com cones de guta-percha e AHPlus. Os dentes foram divididos em dois grupos (n=20): WaveOne Gold 25.07 (WOG) e ProDesign RT 25.08 (PRT) e escaneados em micro-CT para quantificagdo do volume de material obturador remanescente. Os dados foram submetidos á transformando log 10, o teste t de Student foi realizado para contabilizar múltiplas observagoes por amostra, a significáncia foi fixada em 5%. RESULTADOS: O teste t de Student mostrou que ndo houve diferenga no volume de material obturador remanescente entre os dois sistemas nos tergos: apical (p = 0,392), médio (p = 0,065) ou cervical (p = 0,918). CONCLUSÃO: O material obturador remanescente estavapresente em todos os grupos e ambos os sistemas foram semelhantes na remogdo do material obturador radicular nos caninos inferiores.

Efficacy of WaveOne Gold and ProDesign RT systems in removing filling material: a micro-CT analysis / Eficácia dos sistemas WaveOne Gold e ProDesign RT na remopáo material obturador: uma análise micro-CT

ABSTRACT The remaining filling material after retreatment can harbor bacteria and organic tissues that can influence the outcome of the therapy. Aim The aim of this study was to evalúate, by micro-CT, the amount of filling material remaining in the root canal after its removal using WaveOne Gold or ProDesign RT. Material and Method Forty human mandibular canines were instrumented with the ProTaper Next system up to the X2 instrument (25.06) and filled with gutta-percha cones and AHPlus. Teeth were divided into 2 groups (n=20): WaveOne Gold 25.07 (WOG) and ProDesign RT 25.08 (PRT) for filling removal, after which they were scanned in a micro-CT device to quantify the volume of remaining filling material. The data were subjected to log 10 transformation, Student 's t-test was performed to account for multiple observationsper sample, significance was set at 5%. Results Student 's t-test showed that there was no difference between the two systems regarding the volume of remaining filling material in the thirds: apical (p = 0.392), middle (p = 0.065), or cervical (p = 0.918). Conclusión Remaining filling material was present in all groups and both systems were similar in removing root filling material in mandibular canines.

RESUMO A permanencia de material obturador após o retratamento pode abrigar bactérias e tecidos orgánicos que podem influenciar o resultado da terapia. Objetivo O objetivo deste estudo foi avaliar, por micro-CT, a quantidade de material obturador remanescente no canal radicular após a desobturagdo com WaveOne Gold e ProDesign RT. Material e Método Quarenta caninos inferiores humanos foram instrumentados com o sistema ProTaper Next até o instrumento X2 (25.06) e obturados com cones de guta-percha e AHPlus. Os dentes foram divididos em dois grupos (n=20): WaveOne Gold 25.07 (WOG) e ProDesign RT 25.08 (PRT) e escaneados em micro-CT para quantificagdo do volume de material obturador remanescente. Os dados foram submetidos á transformando log10, o teste t de Student foi realizado para contabilizar múltiplas observagoes por amostra, a significáncia foi fixada em 5%. Resultados O teste t de Student mostrou que ndo houve diferenga no volume de material obturador remanescente entre os dois sistemas nos tergos: apical (p = 0,392), médio (p = 0,065) ou cervical (p = 0,918). Conclusao O material obturador remanescente estavapresente em todos os grupos e ambos os sistemas foram semelhantes na remogdo do material obturador radicular nos caninos inferiores.

Intracanal removal and apical extrusion of filling material after retreatment using rotary or reciprocating instruments: A new approach using human cadavers.

AIM: This study compared intracanal removal of filling as well as the frequency and volume of extruded material after retreatment with either HyFlex or Reciproc instruments in mandibular teeth from cadavers. METHODOLOGY: The root canals of 14 pairs of contralateral single-rooted teeth in mandibles of cadavers were instrumented with Reciproc R40 and filled using lateral compaction. The mandibles were scanned in a micro-computed tomographic (micro-CT) device before and after retreatment procedures. The contralateral teeth were assigned to two groups (n = 14) according to the retreatment protocol using either HyFlex or Reciproc instrument systems. In the HyFlex group, the HyFlex Remover instrument was worked 3 mm short of the working length (WL), followed by HyFlex CM 40.04 and 50.04 at the WL. In the Reciproc group, the R50 instrument was worked up at the coronal two thirds, followed by two more cycles until the WL was reached. Pre- and post-operative micro-CT images were analysed for extrusion and intracanal removal of filling material. RESULTS: After retreatment, extrusion of filling material occurred in 11 (78%) and 14 (100%) teeth from HyFlex and Reciproc groups respectively (p > .05). A similar volume of extruded material was observed after retreatment with both systems (p > .05). A significant decrease in the intracanal filling volume was verified after retreatment with both tested systems (p < .05). However, residual filling material was found in all root canals, regardless of the system. The amount of filling material removed (HyFlex = 80.8%; Reciproc = 65.9%) and the operation time was similar between systems (p > .05). CONCLUSIONS: A high frequency of filling material extrusion was observed after retreatment with the two systems in a cadaver model, with no significant difference between them. Both protocols obtained similar efficacy in filling material removal procedures, although none completely cleaned the canals.

Intraradicular Dentinal Changes Following Fiber Postremoval: Micro-computed Tomography Analysis.

Context: Excessive dentine wear during postremoval can occur due to the adhesion between the cement and the post. Aim: The aim is to evaluate dentine wear and microcrack occurrence following the removal of intraradicular fiberglass posts that were previously cemented with two types of resin cement. Methods: Distal roots (n = 38) of lower molars were instrumented, filled, prepared for intraradicular postinstallation, and divided into two groups according to the cement used for the posts: RelyX ARC or RelyX U200. The posts were removed through abrasion using ultrasonic diamond tips, after which the degrees of dentine wear (volume/mm3) and microcrack formation were assessed using microcomputed tomographic imaging. Statistical Analysis Used: The Mann-Whitney U (P ≤ 0.05) and Chi-squared tests (P ≤ 0.05) were used. Results: After the removal of the posts, no statistically significant difference in root canal volume or microcrack occurrence was observed between the groups (P > 0.05). The procedure produced significant dentine wear regardless of the type of cement used (P < 0.05). Conclusions: The removal of fiberglass posts caused extensive dentine wear but did not influence microcrack occurrence. The type of resin cement used did not affect the dentine loss or microcrack appearance that resulted from fiberglass postremoval.

Ontogenetic skull variation in a shovel-headed amphisbaenian species.

Leposternon microcephalum is a species belonging to the Amphisbaenia, a group of burrowing reptiles. Amphisbaenia present various morphological and physiological adaptations that allow them to penetrate the ground and live underground, through a system of galleries and permanent chambers that they build themselves. Among the morphological adaptations in this group, those of the skull stand out as it serves as the main excavation tool. Four basic skull shapes are recognized: rounded, keeled, shovel-shaped, and spade-shaped. The skull of L. microcephalum belongs to this last type, which is considered the most specialized. The species inhabits soils that are highly compacted and difficult to penetrate. Among the species of Leposternon present in South America, L. microcephalum has the widest distribution, being found in all Brazilian biomes and neighboring countries such as Bolivia, Argentina, Paraguay, and Uruguay. The analysis of the skull of this species was carried out using three-dimensional geometric morphometrics (3D-GMM), a technique that allows comparative analysis, through robust statistical methods, of shape and its variations, using Cartesian coordinate data from a configuration of homologous landmarks. The technique allows the size and shape components of a structure to be analyzed separately. From an ontogenetic point of view, this methodology had also been used to investigate variations in Cynisca leucura, a member of the Amphisbaenidae with a rounded head. Our hypothesis is that the patterns of morphological differentiation in the skull, mainly in the intermediate and occipital regions, are similar in different Amphisbaenia species. Therefore, the objective of this study was to analyze cranial morphological variations in an ontogenetic series of L. microcephalum using 3D-GMM. Computed Tomographic scans of 13 specimens were analyzed: juveniles (N = 8) and adults (N = 5), based on 20 landmarks that characterize the skull. Principal components and regression analyses between shape (dependent variable) and size (independent variable) showed a clear difference between the cranial morphological pattern of juvenile individuals and that of adults. For instance, young specimens tend to have a dorsoventrally tall neurocranium, with the tip of the snout more anteriorly oriented and its dorsal border subtly curved. Dorsally, the parietal region is thicker and smoothly dome-shaped in juveniles. As in C. leucura, the variation was strongly correlated with the size change from juvenile to adult, indicating a dominant role for ontogenetic allometry in determining skull shape.

Further development of the LNMRI/IRD's TNF2 thermal system for flexible dose rate irradiation.

The 'Inverse Square Law' (ISL)(  1) requires the existence of a point radiation source to be validated, but in some cases where there is more than one source, its use is possible as long as a point is determined where a virtual source can be positioned and from this point the points correspond to the ISL. Ambient dose equivalent rate values were obtained by simulation and measurements at various points along the LNMRI Thermal Neutron Flux 2 (TNF2) central axis front face, determining a function corresponding to the inverse square of the distance and the font position at the virtual point 'y0', so that the ISL is respected. This function will help in the neutron monitors calibration, previously estimating the ambient dose equivalent at a certain distance from the face.

POLYETHYLENE FILTER FOR THE TNF2 THERMAL NEUTRON FACILITY.

The Standard Thermal Neutron Flux Unit, TNF2, in (LNMRI/IRD)(1,  2), was built for neutron detector and survey meter calibrations. The facility's fluence is achieved by moderation of four 241Am-Be with a graphite core and paraffin/graphite blocks surrounding it. Due to the small channel dimensions, it is impossible to calibrate personal dosemeters and survey meters for thermal neutrons. A polyethylene filter construction was carried out to allow the external irradiation of personal dosemeters and neutron survey meters. The polyethylene filter was constructed with 29 stacked discs with diameters ranging from 5 to 34 cm. Different thicknesses were simulated to provide the desired effect. This new irradiation configuration was also experimentally tested and compared with simulation results with MCNPX(3).

Optimal theranostic SPECT imaging protocol for 223radium dichloride therapy.

223Radium dichloride image-based individual dosimetry requires an optimal acquisition and reconstruction protocol and proper image correction methods for theranostic applications. To assess this problem, radium-223 dichloride SPECT images were acquired from a Jaszczak simulator with a dual-headed gamma camera, LEHR collimator, 128 × 128 matrix, and total time of 32 minutes. A cylindrical PMMA phantom was used to calibrate the measurements performed with Jaszczak. The image quality parameters (noise coefficient, contrast, contrast-to-noise ratio and recovery coefficient) and septal penetration correction were calculated by MATLAB®. The best results for the investigated image quality parameters were obtained with an 89 keV energy window (24% wide) produced together with OSEM/MLEM reconstruction (8 subsets and 4 iterations) applying a Butterworth filter (order 10 and cutoff frequency of 0.48 cycles·cm-1). The successfully performed recovery coefficient parameter evaluation allows uptake correction for future patient dosimetry applications.

Planned Apical Preparation Using Cone-Beam Computed Tomographic Measures: A Micro-Computed Tomographic Proof of Concept in Human Cadavers.

INTRODUCTION: This study assessed the amount of unprepared surface areas at the apical 4-mm segment of the root canal after a planned preparation based on cone-beam computed tomography (CBCT) measurements. METHODS: Eighteen posterior mandible segments were obtained from cadavers and scanned using CBCT and micro-computed tomographic (micro-CT) imaging. CBCT images were used to measure the largest initial canal diameter from 29 root canals of premolars at 1, 2, 3, and 4 mm short of the apical foramen. Each measurement was used to select a master apical instrument with size/taper that was 1 diameter larger to prepare the apical 4 mm of each particular root canal. A post-preparation micro-CT scan was obtained, and the unprepared canal areas were calculated. RESULTS: A very high amount of surface areas over the apical 4 mm of the root canal was included in the final preparation (mean >90%). The unprepared areas ranged from as low as 3.7% to a maximum of 14.6% (mean and median, 9.2% and 9.1%, respectively). CONCLUSIONS: The proposed planned apical root canal preparation resulted in optimized root canal shaping with a substantial amount of prepared surface areas. The protocol used also resulted in a conservative canal enlargement using final instruments that were 1 size larger than the initial largest canal diameter.

The effectiveness of passive ultrasonic irrigation and the easy-clean instrument for removing remnants of filling material.

OBJECTIVE: This study aimed to evaluate the efficacy of passive ultrasonic irrigation (PUI) and the easy clean instrument by micro-computed tomography (CT) for removing remnant filling materials during endodontic retreatment. MATERIALS AND METHODS: Forty mesial roots of mandibular molars were divided into four groups (n = 10) according to the agitation system and sealer used: Group 1: PUI/AH Plus; Group 2: PUI/TotalFill; Group 3: Easy Clean/AH Plus; and Group 4: Easy Clean/TotalFill. The groups were compared by micro-CT analysis according to the volumes of the obturation and the remaining material before and after the agitation systems were used. RESULTS: There was no difference between the groups for total filling volume and initial and final remaining filling volume (P > 0.05). CONCLUSIONS: Both the PUI and easy-clean instrument are effective for removing remnants of filling material with no difference between the groups.