LiDAR Is Effective in Characterizing Vine Growth and Detecting Associated Genetic Loci.

The strong societal demand to reduce pesticide use and adaptation to climate change challenges the capacities of phenotyping new varieties in the vineyard. High-throughput phenotyping is a way to obtain meaningful and reliable information on hundreds of genotypes in a limited period. We evaluated traits related to growth in 209 genotypes from an interspecific grapevine biparental cross, between IJ119, a local genitor, and Divona, both in summer and in winter, using several methods: fresh pruning wood weight, exposed leaf area calculated from digital images, leaf chlorophyll concentration, and LiDAR-derived apparent volumes. Using high-density genetic information obtained by the genotyping by sequencing technology (GBS), we detected 6 regions of the grapevine genome [quantitative trait loci (QTL)] associated with the variations of the traits in the progeny. The detection of statistically significant QTLs, as well as correlations (R2) with traditional methods above 0.46, shows that LiDAR technology is effective in characterizing the growth features of the grapevine. Heritabilities calculated with LiDAR-derived total canopy and pruning wood volumes were high, above 0.66, and stable between growing seasons. These variables provided genetic models explaining up to 47% of the phenotypic variance, which were better than models obtained with the exposed leaf area estimated from images and the destructive pruning weight measurements. Our results highlight the relevance of LiDAR-derived traits for characterizing genetically induced differences in grapevine growth and open new perspectives for high-throughput phenotyping of grapevines in the vineyard.

Analyzing Changes in Maize Leaves Orientation due to GxExM Using an Automatic Method from RGB Images.

The sowing pattern has an important impact on light interception efficiency in maize by determining the spatial distribution of leaves within the canopy. Leaves orientation is an important architectural trait determining maize canopies light interception. Previous studies have indicated how maize genotypes may adapt leaves orientation to avoid mutual shading with neighboring plants as a plastic response to intraspecific competition. The goal of the present study is 2-fold: firstly, to propose and validate an automatic algorithm (Automatic Leaf Azimuth Estimation from Midrib detection [ALAEM]) based on leaves midrib detection in vertical red green blue (RGB) images to describe leaves orientation at the canopy level; and secondly, to describe genotypic and environmental differences in leaves orientation in a panel of 5 maize hybrids sowing at 2 densities (6 and 12 plants.m-2) and 2 row spacing (0.4 and 0.8 m) over 2 different sites in southern France. The ALAEM algorithm was validated against in situ annotations of leaves orientation, showing a satisfactory agreement (root mean square [RMSE] error = 0.1, R2 = 0.35) in the proportion of leaves oriented perpendicular to rows direction across sowing patterns, genotypes, and sites. The results from ALAEM permitted to identify significant differences in leaves orientation associated to leaves intraspecific competition. In both experiments, a progressive increase in the proportion of leaves oriented perpendicular to the row is observed when the rectangularity of the sowing pattern increases from 1 (6 plants.m-2, 0.4 m row spacing) towards 8 (12 plants.m-2, 0.8 m row spacing). Significant differences among the 5 cultivars were found, with 2 hybrids exhibiting, systematically, a more plastic behavior with a significantly higher proportion of leaves oriented perpendicularly to avoid overlapping with neighbor plants at high rectangularity. Differences in leaves orientation were also found between experiments in a squared sowing pattern (6 plants.m-2, 0.4 m row spacing), indicating a possible contribution of illumination conditions inducing a preferential orientation toward east-west direction when intraspecific competition is low.

SegVeg: Segmenting RGB Images into Green and Senescent Vegetation by Combining Deep and Shallow Methods.

Pixel segmentation of high-resolution RGB images into chlorophyll-active or nonactive vegetation classes is a first step often required before estimating key traits of interest. We have developed the SegVeg approach for semantic segmentation of RGB images into three classes (background, green, and senescent vegetation). This is achieved in two steps: A U-net model is first trained on a very large dataset to separate whole vegetation from background. The green and senescent vegetation pixels are then separated using SVM, a shallow machine learning technique, trained over a selection of pixels extracted from images. The performances of the SegVeg approach is then compared to a 3-class U-net model trained using weak supervision over RGB images segmented with SegVeg as groundtruth masks. Results show that the SegVeg approach allows to segment accurately the three classes. However, some confusion is observed mainly between the background and senescent vegetation, particularly over the dark and bright regions of the images. The U-net model achieves similar performances, with slight degradation over the green vegetation: the SVM pixel-based approach provides more precise delineation of the green and senescent patches as compared to the convolutional nature of U-net. The use of the components of several color spaces allows to better classify the vegetation pixels into green and senescent. Finally, the models are used to predict the fraction of three classes over whole images or regularly spaced grid-pixels. Results show that green fraction is very well estimated (R 2 = 0.94) by the SegVeg model, while the senescent and background fractions show slightly degraded performances (R 2 = 0.70 and 0.73, respectively) with a mean 95% confidence error interval of 2.7% and 2.1% for the senescent vegetation and background, versus 1% for green vegetation. We have made SegVeg publicly available as a ready-to-use script and model, along with the entire annotated grid-pixels dataset. We thus hope to render segmentation accessible to a broad audience by requiring neither manual annotation nor knowledge or, at least, offering a pretrained model for more specific use.

Genomic Prediction of Green Fraction Dynamics in Soybean Using Unmanned Aerial Vehicles Observations.

With the widespread use of high-throughput phenotyping systems, growth process data are expected to become more easily available. By applying genomic prediction to growth data, it will be possible to predict the growth of untested genotypes. Predicting the growth process will be useful for crop breeding, as variability in the growth process has a significant impact on the management of plant cultivation. However, the integration of growth modeling and genomic prediction has yet to be studied in depth. In this study, we implemented new prediction models to propose a novel growth prediction scheme. Phenotype data of 198 soybean germplasm genotypes were acquired for 3 years in experimental fields in Tottori, Japan. The longitudinal changes in the green fractions were measured using UAV remote sensing. Then, a dynamic model was fitted to the green fraction to extract the dynamic characteristics of the green fraction as five parameters. Using the estimated growth parameters, we developed models for genomic prediction of the growth process and tested whether the inclusion of the dynamic model contributed to better prediction of growth. Our proposed models consist of two steps: first, predicting the parameters of the dynamics model with genomic prediction, and then substituting the predicted values for the parameters of the dynamics model. By evaluating the heritability of the growth parameters, the dynamic model was able to effectively extract genetic diversity in the growth characteristics of the green fraction. In addition, the proposed prediction model showed higher prediction accuracy than conventional genomic prediction models, especially when the future growth of the test population is a prediction target given the observed values in the first half of growth as training data. This indicates that our model was able to successfully combine information from the early growth period with phenotypic data from the training population for prediction. This prediction method could be applied to selection at an early growth stage in crop breeding, and could reduce the cost and time of field trials.

A Double Swath Configuration for Improving Throughput and Accuracy of Trait Estimate from UAV Images.

Multispectral observations from unmanned aerial vehicles (UAVs) are currently used for precision agriculture and crop phenotyping applications to monitor a series of traits allowing the characterization of the vegetation status. However, the limited autonomy of UAVs makes the completion of flights difficult when sampling large areas. Increasing the throughput of data acquisition while not degrading the ground sample distance (GSD) is, therefore, a critical issue to be solved. We propose here a new image acquisition configuration based on the combination of two focal length (f) optics: an optics with f = 4.2 mm is added to the standard f = 8 mm (SS: single swath) of the multispectral camera (DS: double swath, double of the standard one). Two flights were completed consecutively in 2018 over a maize field using the AIRPHEN multispectral camera at 52 m altitude. The DS flight plan was designed to get 80% overlap with the 4.2 mm optics, while the SS one was designed to get 80% overlap with the 8 mm optics. As a result, the time required to cover the same area is halved for the DS as compared to the SS. The georeferencing accuracy was improved for the DS configuration, particularly for the Z dimension due to the larger view angles available with the small focal length optics. Application to plant height estimates demonstrates that the DS configuration provides similar results as the SS one. However, for both the DS and SS configurations, degrading the quality level used to generate the 3D point cloud significantly decreases the plant height estimates.

Narrowing uncertainties in the effects of elevated CO2 on crops.

Plant responses to rising atmospheric carbon dioxide (CO2) concentrations, together with projected variations in temperature and precipitation will determine future agricultural production. Estimates of the impacts of climate change on agriculture provide essential information to design effective adaptation strategies, and develop sustainable food systems. Here, we review the current experimental evidence and crop models on the effects of elevated CO2 concentrations. Recent concerted efforts have narrowed the uncertainties in CO2-induced crop responses so that climate change impact simulations omitting CO2 can now be eliminated. To address remaining knowledge gaps and uncertainties in estimating the effects of elevated CO2 and climate change on crops, future research should expand experiments on more crop species under a wider range of growing conditions, improve the representation of responses to climate extremes in crop models, and simulate additional crop physiological processes related to nutritional quality.

[Descriptive analysis of C-Reactive values after uncomplicated total hip and knee arthroplasty]. / Análisis descriptivo de los niveles plasmáticos de Proteína C-Reactiva tras artroplastía total de cadera y rodilla no complicada.

Serum C-Reactive Protein was measured serially in 45 patients treated with uncomplicated primary total hip and knee arthroplasty (24 THR y 21 TKR). Blood specimens were obtained before surgery, on surgery day and on the 2nd, 3rd, 5th, 13th, 42nd and 150th postoperative days. In both groups serum CRP levels increased rapidly after surgery, with maximum levels on second postoperative day, higher in the TKR group and decreased gradually to preoperative levels on day 150, but still high on 42nd day. Levels returned to normal after surgery at the same time in both groups and no significant differences were found. Rising CRP levels after the third postoperative day may suggest a surgery complication such as infection.

Comparison of mobile- and fixed-bearing cemented total knee arthroplasty.

Concern about polyethylene wear and related osteolysis after knee arthroplasty has developed in the last years. Mobile-bearing knee prostheses were designed in order to reduce the influence of this critical factor on long-term success of total knee replacement. We present a prospective study comparing clinical and radiological results with a mobile-bearing (Ceragyr) and a fixed-bearing knee prosthesis (posterior stabilized Hermes). Clinical results did not show any significant differences in Knee Society scores. We found better results in the mobile-bearing group for pain scores and subjective preference, but the difference did not reach statistical significance. Within the time limits of this study, radiological analysis showed no osteolysis in either group, but longer follow-up will be needed to confirm this.