RESUMO
Anestrus poses substantial challenges for dairy industries worldwide. This research aims to evaluate the effect of oral supplementation with garlic alone, vitamins A, D3, E alone, and their combination on heat detection, pregnancy rate and serum profile in anestrus dairy cows. Using a completely randomized design, this study, conducted over 327 days, evaluated 72 anestrus-affected cows, which were divided into four groups. T0 (Control) received only normal saline; T1 with vitamin A,D3,E; T2 with Garlic and T3 integrated with A, D3, E + Garlic in drinking water. Results demonstrated that the treatment groups exhibited significantly higher estrus signs (p < 0.05) compared to the control. The pregnancy rate was also increased (P = 0.059), especially in the T3 group which had the highest heat detection (55.55 %), conception (70 %), and pregnancy (38.88 %). In comparison, the heat detection rate for T1 was 44.44 %, while the conception rate for T2 was 66.66 %. Additionally, T0 exhibited the least proportion across all parameters. Additionally, the elevated BUN (Blood Urea Nitrogen) and blood glucose levels observed in non-responsive (those not showing signs of heat) cows at 32.51 and 43.65 mg/dl respectively, compared to responsive cows (those showing signs of heat) with levels of 22.40 and 51.34 mg/dl. These findings suggest a potential association between these blood parameters and the cyclicity of dairy cows. These findings highlight that oral supplementation with vitamin AD3E+Garlic significantly improves the heat detection rate and pregnancy rate than only administering vitamin AD3E or Garlic with anestrus in dairy cattle.