Small Molecule-Inducible and Photoactivatable Cellular RNA N1-Methyladenosine Editing.

N1-methyladenosine (m1A) modification is one of the most prevalent epigenetic modifications on RNA. Given the vital role of m1A modification in RNA processing such as splicing, stability and translation, developing a precise and controllable m1A editing tool is pivotal for in-depth investigating the biological functions of m1A. In this study, we developed an abscisic acid (ABA)-inducible and reversible m1A demethylation tool (termed AI-dm1A), which targets specific transcripts by combining the chemical proximity-induction techniques with the CRISPR/dCas13b system and ALKBH3. We successfully employed AI-dm1A to selectively demethylate the m1A modifications at A8422 of MALAT1 RNA, and this demethylation process could be reversed by removing ABA. Furthermore, we validated its demethylation function on various types of cellular RNAs including mRNA, rRNA and lncRNA. Additionally, we used AI-dm1A to specifically demethylate m1A on ATP5D mRNA, which promoted ATP5D expression and enhanced the glycolysis activity of tumor cells. Conversely, by replacing the demethylase ALKBH3 with methyltransferase TRMT61A, we also developed a controllable m1A methylation tool, namely AI-m1A. Finally, we caged ABA by 4,5-dimethoxy-2-nitrobenzyl (DMNB) to achieve light-inducible m1A methylation or demethylation on specific transcripts. Collectively, our m1A editing tool enables us to flexibly study how m1A modifications on specific transcript influence biological functions and phenotypes.

N6-methyladenosine facilitates mitochondrial fusion of colorectal cancer cells via induction of GSH synthesis and stabilization of OPA1 mRNA.

Mitochondria undergo fission and fusion that are critical for cell survival and cancer development, while the regulatory factors for mitochondrial dynamics remain elusive. Herein we found that RNA m6A accelerated mitochondria fusion of colorectal cancer (CRC) cells. Metabolomics analysis and function studies indicated that m6A triggered the generation of glutathione (GSH) via the upregulation of RRM2B-a p53-inducible ribonucleotide reductase subunit with anti-reactive oxygen species potential. This in turn resulted in the mitochondria fusion of CRC cells. Mechanistically, m6A methylation of A1240 at 3'UTR of RRM2B increased its mRNA stability via binding with IGF2BP2. Similarly, m6A methylation of A2212 at the coding sequence (CDS) of OPA1-an essential GTPase protein for mitochondrial inner membrane fusion-also increased mRNA stability and triggered mitochondria fusion. Targeting m6A through the methyltransferase inhibitor STM2457 or the dm6ACRISPR system significantly suppressed mitochondria fusion. In vivo and clinical data confirmed the positive roles of the m6A/mitochondrial dynamics in tumor growth and CRC progression. Collectively, m6A promoted mitochondria fusion via induction of GSH synthesis and OPA1 expression, which facilitated cancer cell growth and CRC development.

Quasi-TPPs/Fano resonance systems based on an MDM waveguide structure and its sensing application.

In this paper, quasi-Tamm plasmon polaritons (TPPs)/Fano resonance systems based on metal-dielectric-metal (MDM) waveguides are proposed. TPPs are surface electromagnetic modes formed at the interface between a metal and a one-dimensional dielectric photonic crystal (PhC). A metal plasmonic Bragg reflector (PBR) in a MDM waveguide is equivalent to a dielectric PhC, which is realized by periodic MDM waveguide width modulation and leads to the photonic bandgap. By introducing a thin Ag baffle and a PBR in MDM waveguide core, the quasi-TPPs are excited at the interface between the Ag baffle and the PBR, when the phase-matching condition is met. The proposed structure can be fabricated with focused ion beam or electron beam direct-writing lithography, avoiding complex fabrication procedures of manufacturing dielectric PhC by filling the MDM waveguide core with different dielectric materials. Furthermore, an MDM waveguide side-coupled resonator system is constructed to generate Fano resonance by placing a PBR on the side of the MDM waveguide and an Ag baffle in the waveguide core. The Fano resonance originates from the interference between a broad continuum state provided by the Ag baffle and a discrete state provided by quasi-TPPs. The sensing performance of the Fano resonance system is investigated. In this design, the open PBR structure replaces the traditional closed resonant cavity, which makes it more convenient to contact with analytes. The numerical simulations demonstrate that a high sensitivity of 1500 nm/RIU and figure of merit value of 4.08×105 are achieved.

Ultracompact parabolic sidewall-profiled hybrid plasmonic waveguide Bragg grating with optimized spectral properties of long-wavelength passband.

An ultracompact hybrid plasmonic waveguide Bragg grating (HPWBG) with improved spectral properties of long-wavelength passband is proposed. A hollow HPW is introduced to suppress the entire loss, and a parabolic profiled sidewall is designed to optimize the spectral properties for specific wave bands. The transfer matrix method and finite element method are combined to ensure the efficiency of numerical research. The results show that the parabolic profile effectively reduces the reflection and strengthens the resonance of the mode in the long-wavelength passband, suppressing the oscillations and realizing significant smoothness and improvement in transmission. The optimized transmittance is greater than 99%, and insertion loss is as low as 0.017 dB. A wide bandgap of 103 nm is also attained. The structure also has a compactness with a length of 3.4 µm and exhibits good tolerance. This work provides a scheme for designing and optimizing wavelength selecting devices and has potential application value in integrated photonic devices.

Compact and low-loss TM-pass polarizer based on a hybrid plasmonic waveguide with a semiround arch Si core.

A compact and low loss TM-pass polarizer based on a hybrid plasmonic waveguide (HPW) has been demonstrated. By introducing the hollow HPW with a semiround arch (SRA) Si core, the unwanted TE mode can be effectively cut off and the TM mode can pass through by hybrid plasmonic mode with excellent transmission characteristics. The hollow structure realizes lower index with n=1 due to the air region, and the SRA construction effectively suppresses the energy loss of the TM mode caused by the corner effect. Thus, TM modes pass through with negligible loss and exhibit the characteristic of strong mode limitation. By optimizing the width of metal, the width of the HPW, and the length of the tapered mode converter, an optimum performance with a high polarization extinction ratio of 67.87 dB and a low insert loss of 0.029 dB at the work wavelength=1550nm is achieved. Detailed analysis also proves that the proposed polarizer has a compact size of only 7 µm and a great fabrication tolerance. This work offers a simple and effective scheme of polarization control on-chip.

Chinese Medicine, Succinum, Ameliorates Cognitive Impairment of Carotid Artery Ligation Rats and Inhibits Apoptosis of HT22 Hippocampal Cells via Regulation of the GSK3ß/ß-Catenin Pathway.

Succinum is an organic mineral formed from the resin of ancient coniferous and leguminous plants, which is applied for tranquilizing mood, promoting blood circulation, and removing blood stasis in Chinese medicine. For quite a long time, the modern research of succinum mainly focuses on the study of physical and chemical properties and authenticity identification while few reports on its medicinal mechanism. In current study, we evaluated different solvent extracts of succinum on carotid artery ligation rats mimicking vascular dementia. It was found that ethyl acetate extracts of succinum significantly improved the learning and memory abilities of model rats and inhibited neuronal apoptosis in the hippocampus. On a mice hippocampal neuronal cell line (HT22), ethyl acetate extracts of succinum also exerted better action trend in inhibiting cell apoptosis induced by oxygen glucose deprivation (OGD). By using XAV-939 on both in vivo and in vitro studies, it was found that ethyl acetate extracts of succinum might exert these functions by regulating the GSK3ß/ß-catenin pathway. These studies revealed the neuronal function of succinum, which explained the traditional effects of succinum and provided more modern scientific basis for its clinical application.

A Multimodal Classification Architecture for the Severity Diagnosis of Glaucoma Based on Deep Learning.

Glaucoma is an optic neuropathy that leads to characteristic visual field defects. However, there is no cure for glaucoma, so the diagnosis of its severity is essential for its prevention. In this paper, we propose a multimodal classification architecture based on deep learning for the severity diagnosis of glaucoma. In this architecture, a gray scale image of the visual field is first reconstructed with a higher resolution in the preprocessing stage, and more subtle feature information is provided for glaucoma diagnosis. We then use multimodal fusion technology to integrate fundus images and gray scale images of the visual field as the input of this architecture. Finally, the inherent limitation of convolutional neural networks (CNNs) is addressed by replacing the original classifier with the proposed classifier. Our architecture is trained and tested on the datasets provided by the First Affiliated Hospital of Kunming Medical University, and the results show that the proposed architecture achieves superior performance for glaucoma diagnosis.

Peripapillary vessel density correlates with visual field mean sensitivity in highly myopic eyes.

PURPOSE: To identify the global and regional distribution of peripapillary vessel density (pVD) and try to find out the relationships between pVD and the visual field mean sensitivity (VFMS) in healthy myopic eyes. DESIGN: Prospective cross-sectional study. METHODS: Two hundred and twenty-two participants (393 eyes) with myopia (myopic refractive error < - 0.5 diopters) from two clinical centers were recruited in this study and were divided into 4 groups according to the spherical equivalent (SE): Group1:- 0.5D ≥ SE > - 6.00D, Group2: - 6.00D ≥ SE > - 8.00D, Group3:- 8.00D ≥ SE > - 10.00D, Group4:SE ≤ -10.00D.The pVD assessed with optical coherence tomography angiography (OCTA) was quantified in 8 sectors. Peripapillary retinal nerve fibre layer (pRNFL) imaging was performed with SD-OCT. Visual field (VF) tests were performed with the 30-2 SITA standard program on the Humphrey 750i Visual Field Analyzer and were grouped into 8 regions that matched the structure. RESULTS: The pRNFL had no significant difference in all groups (p = 0.422). The average pVD were significantly lower in group 4 (47.61 ± 6.58) than in group 2 and 3 (51.49 ± 3.21, 50.48 ± 3.43 respectively) (p < 0.05). While both pVD in group2 and 3 were statistically lower than group1 (52.77 ± 2.86). The average VFMS was significantly lower in group 4 (901.85 ± 386.54) than other three groups (1169.15 ± 328.94, 1081.77 ± 338.83, 1076.89 ± 358.18, for group1,2,3 respectively). The pVD and VFMS were positively correlated in group3 (r = 0.184) and group4 (r = 0.476) (p < 0.05). Linear regression analysis demonstrated that VFMS were positively associated with pVD especially in temporal and nasal quadrants in myopic eyes. CONCLUSIONS: The pVD shows a significant positive correlation with VFMS in highly myopic eyes with SE ≤ - 8.00D. We suggest that pVD measurement by OCTA could be a sensitive and useful method for monitoring myopic functional change.

Focusing characteristics of cylindrical vector beams through a multi-focal all-dielectric grating lens.

A novel, to the best of our knowledge, type of multi-focal all-dielectric grating lens is proposed in this work, and focusing characteristics of cylindrical vector beams through the lens are investigated in detail. Based on the negative refraction mechanism of negative-first-order diffraction and Fermat's principle, a multi-focal lens is designed. By analyzing the diffraction effect of the grating, the essential factor that affects the focus quality is found. Through a two-step optimization process, secondary foci and the focal displacement of primary foci caused by high-order diffractions are overcome, and the quality of the focal field is significantly improved. This work provides a reference for micro-lens design for focus modulation, and the research results also have potential applications in the fields of light-field manipulation and optical tweezers.

Feasible polarised white-light emission based on conjugate plane-structured yellow/blue dye molecules encapsulated in metal-organic frameworks.

We use a two-stage hierarchical growth method to encapsulate the blue KSN and yellow RhB molecules into a MOF crystal. By aligning these two conjugate plane-structured molecules in the MOF channel, a polarised white-light emission is obtained, with CIE coordinates of (0.3285, 0.3204) and a polarization ratio of 2.98.

Compact general interference hybrid-plasmonic multimode interferometers used for optical hybrid.

We propose three general interference multimode interferometers (MMIs) based on hybrid plasmonic waveguides (HPWs). Among them, the general 2×2 and 4×4 MMIs are designed for a 90° optical hybrid, while the 3×3 MMI is for a 120° optical hybrid. First, by considering the mode interference characteristics inside the multimode HPWs, a compromise between the number of guided modes and the device length is obtained at a determined height of the SiO2 interlayer of the HPW. Also, by analyzing the characteristics of multimode propagation in the HPW-MMI, it is found that the optimal positions of self-images would shift from their theoretical ones. In addition, tapered HPW sections are implemented to improve the coupling efficiencies for lights coupled into/out of the multimode section. Therefore, by optimizing the width and length of the multimode section, and especially the position of the input and output single-mode waveguides, the appropriate structure parameters of three HPW-MMIs are obtained, where the footprints of the 2×2, 3×3, and 4×4 HPW-MMIs are only 1.96×5.4 µm2, 2.18×12.0 µm2, and 2.52×11.5 µm2, respectively. The simulation results show that, at the wavelength of 1550 nm, the 2×2 HPW-MMI exhibits a transmission of 75.6%, a maximum transmissions imbalance of 0.55 dB, and a phase error of 3.68°; the 3×3 HPW-MMI exhibits a transmission of 69.2%, a maximum transmissions imbalance of 0.43 dB, and a phase error of 4.66°; and the 4×4 HPW-MMI exhibits a transmission of 68.5%, a maximum transmissions imbalance of 0.91 dB, and a phase error of 4.81°. All these performances meet the standard industry requirements.

Field-enhanced nanofocusing of radially polarized light by a tapered hybrid plasmonic waveguide with periodic grooves.

This study reports the field-enhanced nanofocusing of radially polarized light by tapered hybrid plasmonic waveguide (THPW) with periodic grooves. The THPW consists of a conical high-index dielectric cone, a sandwiched low-index dielectric thin layer, and a metal cladding. The axially symmetric 3D finite element method is used to investigate the nanofocusing effect. Under radially polarized illumination at 632.8 nm, strongly enhanced nanofocusing occurs. The hybrid plasmonic structure effectively reduces the energy loss and improves the field enhancement nearly 554 times. Furthermore, periodic grooves are constructed on the metallic surface of the THPW, satisfying the phase-matching condition, and they couple the light energy from the inside to the outside. Finally, an optimized nanofocusing performance with field enhancement of approximately 1810 times is obtained. The results offer an important reference for designing related photonic devices, and the proposed scheme could be potentially exploited in the application of light-matter interactions.

Adsorption and mechanistic study for phosphate removal by magnetic Fe3O4-doped spent FCC catalysts adsorbent.

The waste materials utilization has attained increasing attention due to the generation of a large number of spent materials. In the current study, a practical magnetic adsorbent (Fe3O4-doped spent Fluid Catalytic Cracking catalysts, abbreviated as FCCx@(Fe)y-O) was prepared, liable to be separated. The batch experiments were employed to investigate the phosphate removal behavior. The findings of this study demonstrated that FCC4@(Fe)1-O exhibited the best phosphate removal performance among the adsorbents (FCCx@(Fe)y-O), attributed to rough surface layer, i.e., composed of active sites. The various characterizations results revealed that the adsorption behavior of FCC4@(Fe)1-O followed the inner-sphere adsorption based on ligand exchanges mechanism. Furthermore, OH- played an important role in the adsorption process. Minor effects were showed on the phosphate removal in the experiments of commonly coexisting anions, except CO32- and SiO32-. The above findings affirmed that FCC4@(Fe)1-O was a suitable adsorbent for phosphate removal in the practical application.

TM01 mode in a cylindrical hybrid plasmonic waveguide with large propagation length.

This study reports on a cylindrical hybrid plasmonic waveguide (CHPW) consisting of a high-index dielectric core, a sandwiched low-index dielectric layer, and a metal cladding. The CHPW supports the TM01 mode with a radially polarized transverse component of the electric field. Optical fields can be significantly enhanced in the sandwiched low-index dielectric region with nanoscale thickness down to 5 nm, and tight mode confinement with the same order of the normalized mode area compared with that of the plasmonic waveguide is achieved. Moreover, the mode propagation loss is well compensated by adjusting dimensions of the waveguide to obtain a longer propagation distance. The calculated figure of merit reaches a value several times greater than that in the similarly reported structure. The results indicate that this novel type of hybrid structure can support the limited propagation of a radially polarized mode with good confinement and low loss, consummate the whole system of manipulating the cylindrical vector beams, and show great potential of applications for various integrated nano-photonic devices.

Focus modulation of cylindrical vector beams by using 1D photonic crystal lens with negative refraction effect.

Sub-wavelength focusing of cylindrical vector beams (CVBs) has attracted great attention due to the specific physical effects and the applications in many areas. More powerful, flexible and effective ways to modulate the focus transversally and also longitudinally are always being pursued. In this paper, cylindrically symmetric lens composed of negative-index one-dimensional photonic crystal is proposed to make a breakthrough. By revealing the relationship between focal length and the exit surface shape of the lens, a quite simple and effective principle of designing the lens structure is presented to realize specific focus modulation. Plano-concave lenses are parameterized to modulate the focal length and the number of focuses. An axicon constructed by one-dimensional photonic crystal is proposed for the first time to obtain a large depth of focus and an optical needle focal field with almost a theoretical minimum FWHM of 0.362λ is achieved under radially polarized incident light. Because of the almost identical negative refractive index for TE and TM polarization states, all the modulation methods can be applied for any arbitrary polarized CVBs. This work offers a promising methodology for designing negative-index lenses in related application areas.

Facile fabrication of three-dimensional graphene foam/poly(dimethylsiloxane) composites and their potential application as strain sensor.

A three-dimensional (3D) graphene foam (GF)/poly(dimethylsiloxane) (PDMS) composite was fabricated by infiltrating PDMS into 3D GF, which was synthesized by chemical vapor deposition (CVD) with nickel foam as template. The electrical properties of the GF/PDMS composite under bending stress were investigated, indicating the resistance of the GF/PDMS composite was increased with the bending curvature. To improve the bending sensitivity of the GF/PDMS composite, a thin layer of poly(ethylene terephthalate) (PET) was introduced as substrate to form double-layer GF/PDMS-PET composite, whose measurements showed that the resistance of the GF/PDMS-PET composite was still increased when bended to the side of PET, whereas its resistance would be decreased when bended to the side of GF. For both cases, the absolute value of the relative variation of electrical resistance was increased with the bending curvature. More importantly, the relative variation of electrical resistance for double-layer GF/PDMS-PET composite can be up to six times higher than single-layer GF/PDMS composite for the same bending curvature. These observations were further supported by the principle of mechanics of material. The 3D GF/PDMS-PET composite also has higher flexibility and environment stability and can be utilized as a strain sensor with high sensitivity, which can find important applications in real-time monitoring of buildings, such as a bridge, dam, and high-speed railway.

Polarization-dependent Talbot effect.

The term "polarization-dependent Talbot effect" means that the Talbot self-imaging intensity of a high-density grating is different for TE and TM polarization modes. Numerical simulations with the finite-difference time-domain method show that the polarization dependence of the Talbot images is obvious for gratings with period d between 2 lambda and 3 lambda. Such a polarization-dependent difference for TE and TM polarization of a high-density grating of 630 lines/mm (corresponding to d/lambda=2.5) is verified through experiments with the scanning near-field optical microscopy technique, in which a He-Ne laser is used as its polarization is changed from the TE mode to the TM mode. The polarization-dependent Talbot effect should help us to understand more clearly the diffraction behavior of a high-density grating in nano-optics and contribute to wide application of the Talbot effect.

Talbot effect of a grating with different kinds of flaws.

The Talbot effect of a grating with different kinds of flaws is analyzed with the finite-difference time-domain (FDTD) method. The FDTD method can show the exact near-field distribution of different flaws in a high-density grating, which is impossible to obtain with the conventional Fourier transform method. The numerical results indicate that if a grating is perfect, its Talbot imaging should also be perfect; if the grating is distorted, its Talbot imaging will also be distorted. Furthermore, we evaluate high-density gratings by detecting the near-field distribution with the scanning near-field optical microscopy technique. Experimental results are also given.