RESUMO
Juvenile clearnose skates (Raja eglanteria) were injected intramuscularly with dexamethasone-21-phosphate at 50, 75, and 100mg/kg body weight. After 24h, skates were sacrificed and lymphomyeloid tissues (thymus, spleen, Leydig organ, and epigonal organ) were removed and whole blood was sampled. Tissues were used fresh for imprints or prepared for histology by solvent fixation or freezing in liquid nitrogen. Apoptosis in fixed tissues was assessed by transmission electron microscopy. Frozen sections and cytospin preparations of peripheral blood leukocytes (PBL) were evaluated by the TUNEL reaction to detect DNA strand breaks. Dexamethasone treatment increased apoptotic activity in all lymphomyeloid tissues as well as in PBL. These studies demonstrate that immune cells of elasmobranchs have the capacity for glucocorticoid-driven apoptosis, and that programmed cell death as a mechanism to regulate immune cell production appears to have been conserved during vertebrate evolution.
Assuntos
Apoptose , Dexametasona/análogos & derivados , Dexametasona/farmacologia , Glucocorticoides/farmacologia , Leucócitos Mononucleares/efeitos dos fármacos , Células Intersticiais do Testículo/efeitos dos fármacos , Baço/efeitos dos fármacos , Timo/efeitos dos fármacos , Animais , Dexametasona/administração & dosagem , Glucocorticoides/administração & dosagem , Gônadas/citologia , Gônadas/efeitos dos fármacos , Leucócitos Mononucleares/citologia , Células Intersticiais do Testículo/citologia , Tecido Linfoide/citologia , Tecido Linfoide/efeitos dos fármacos , Masculino , Rajidae , Baço/citologia , Timo/citologiaRESUMO
Cartilaginous fish express canonical B and T cell recognition genes, but their lymphoid organs and lymphocyte development have been poorly defined. Here, the expression of Ig, TCR, recombination-activating gene (Rag)-1 and terminal deoxynucleosidase (TdT) genes has been used to identify roles of various lymphoid tissues throughout development in the cartilaginous fish, Raja eglanteria (clearnose skate). In embryogenesis, Ig and TCR genes are sharply up-regulated at 8 weeks of development. At this stage TCR and TdT expression is limited to the thymus; later, TCR gene expression appears in peripheral sites in hatchlings and adults, suggesting that the thymus is a source of T cells as in mammals. B cell gene expression indicates more complex roles for the spleen and two special organs of cartilaginous fish-the Leydig and epigonal (gonad-associated) organs. In the adult, the Leydig organ is the site of the highest IgM and IgX expression. However, the spleen is the first site of IgM expression, while IgX is expressed first in gonad, liver, Leydig and even thymus. Distinctive spatiotemporal patterns of Ig light chain gene expression also are seen. A subset of Ig genes is pre-rearranged in the germline of the cartilaginous fish, making expression possible without rearrangement. To assess whether this allows differential developmental regulation, IgM and IgX heavy chain cDNA sequences from specific tissues and developmental stages have been compared with known germline-joined genomic sequences. Both non-productively rearranged genes and germline-joined genes are transcribed in the embryo and hatchling, but not in the adult.
Assuntos
Rajidae/genética , Animais , Linfócitos B , DNA Nucleotidilexotransferase/genética , Expressão Gênica , Gônadas/imunologia , Proteínas de Homeodomínio/genética , Cadeias Leves de Imunoglobulina/genética , Imunoglobulina M/genética , Imunoglobulinas/genética , Reação em Cadeia da Polimerase , Receptores de Antígenos de Linfócitos T alfa-beta/genética , Receptores de Antígenos de Linfócitos T gama-delta/genética , Rajidae/crescimento & desenvolvimento , Rajidae/imunologia , Baço/imunologia , Timo/imunologia , Transposases/genéticaRESUMO
Serum steroid hormones in the peripheral circulation of the clearnose skate, Raja eglanteria, were measured at the time of capture and at various times throughout the year while the animals were maintained as a captive breeding population. These analyses demonstrate interesting correlations between changes in hormone concentrations and annual reproductive events. Animals were sampled once (78 females, 20 males) or multiple times (15 females). For both groups of females, 17beta-estradiol was detected throughout the year with significant elevations occurring during October and November when ovarian follicles begin to mature (as determined through necropsy examinations), and January and February when maximum mating activity is observed and egg laying begins. Testosterone and dihydrotestosterone concentrations were significantly elevated in females only during January and February. Testosterone elevations were synchronous with longer-term elevations in 17beta-estradiol in females sampled either once or repetitively. Testosterone concentrations in males were significantly elevated during times of maximum breeding activity compared to periods of sexual inactivity. Data from females sampled during five stages of the egg laying process, as defined by the position of palpable egg capsules within the reproductive tract, revealed that 17beta-estradiol was highest when egg capsules were forming in the nidamental gland (stage 2) or uterus (stage 3); testosterone and dihydrotestosterone were maximal when eggs were in the uterus (stage 3) or cloaca (stage 4); and progesterone was significantly elevated immediately after oviposition (stage 5), suggesting a possible role for progesterone in the regulation of sequential laying of egg pairs. J. Exp. Zool. 284:575-585, 1999.
Assuntos
Hormônios Esteroides Gonadais/sangue , Reprodução/fisiologia , Estações do Ano , Rajidae/fisiologia , Animais , Di-Hidrotestosterona/sangue , Estradiol/sangue , Feminino , Masculino , Progesterona/sangue , Radioimunoensaio , Testosterona/sangueRESUMO
Differential screening has been used to identify cDNAs encoding a long form of IgX in Raja eglanteria (clearnose skate). Comparisons of the IgX long form with the previously described short-form IgX cDNAs and the genomic IgX locus indicate that the V and two 5' C regions of the short and long forms of IgX are >90% identical at the nucleotide level. Differences between the V sequences of the long- and short-form IgX genes are concentrated in complementarity determining regions, suggesting that these forms are derived through alternative splicing of the same genomic loci or transcription of highly related loci. The extreme conservation of nucleotide sequence, including third position codons, among different cDNAs as well as the near identity of nucleotide sequence in the intervening sequences of germline IgX, IgX short-form sterile transcripts and IgX long-form sterile transcripts indicate that the multiple IgX loci are recently diverged from one another and/or are under intense gene correction. Phylogenetic analyses of the known cartilaginous fish immunoglobulin loci demonstrate that the long form of IgX is orthologous to IgW/IgNARC (NARC) and is most consistent with: 1) the divergence of the IgX/IgW/NARC and IgM-like loci from a common ancestral locus prior to the divergence of the cartilaginous/bony fish lineages and 2) the divergence of the NAR locus from the IgX/IgW/NARC gene(s) after the cartilaginous/bony fish split but prior to the shark/skate split, approximately 220 million years ago.
Assuntos
Genes de Imunoglobulinas , Cadeias Pesadas de Imunoglobulinas/genética , Tubarões/genética , Rajidae/genética , Sequência de Aminoácidos , Animais , Diversidade de Anticorpos , Sequência de Bases , DNA Complementar/genética , Evolução Molecular , Peixes/classificação , Peixes/genética , Isotipos de Imunoglobulinas , Mamíferos/genética , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Rajidae/imunologia , Especificidade da Espécie , Transcrição GênicaRESUMO
This study examined the response properties of skate electrosensory primary afferent neurons of pre-hatch embryo (8-11 weeks), post-hatch juvenile (1-8 months), and adult (> 2 year) clearnose skates (Raja eglanteria) to determine whether encoding of electrosensory information changes with age, and if the electrosense is adapted to encode natural bioelectric stimuli across life history stages. During ontogeny, electrosensory primary afferents increase resting discharge rate, spike regularity, and sensitivity at best frequency. Best frequency was at 1-2 Hz for embryos, showed an upwards shift to 5 Hz in juveniles, and a downward shift to 2-3 HZ in adults. Encapsulated embryos exhibit ventilatory movements that are interrupted by a "freeze response" when presented with weak uniform fields at 0.5 and 1 Hz. This phasic electric stimulus contains spectral information found in potentials produced by natural fish predators, and therefore indicates that the embryo electrosense can efficiently mediate predator detection and avoidance. In contrast, reproductively active adult clearnose skates discharge their electric organs at rates near the peak frequency sensitivity of the adult electrosensory system, which; facilitates electric communication during social behavior. We suggest that life-history-dependent functions such as these may shape the evolution of the low-frequency response properties for the elasmobranch electrosensory system.
Assuntos
Sensação/fisiologia , Rajidae/fisiologia , Comunicação Animal , Animais , Estimulação Elétrica , Embrião não Mamífero/fisiologia , Feminino , Masculino , Neurônios Aferentes/fisiologia , Rajidae/crescimento & desenvolvimento , Comportamento SocialRESUMO
Volume expansion of cardiac cells from a wide variety of species stimulates the efflux of the beta-amino acid taurine through an osmolyte channel. Previous studies have suggested that the osmolyte channel in epithelial cells is a swelling-activated anion channel (pICln). In skate heart, a 37-kDa protein is present which is recognized by a specific antibody to a protein characterized in MDCK cells as pICln. This protein is present predominantly in the cytosol (only 10% in the membrane fraction) of heart incubated under isotonic conditions. After transfer to hypotonic medium (one-half osmolarity), the distribution of this protein is markedly altered and significant amounts of the protein are found in the membrane fraction. After hypotonic exposure, the amount of the protein in the membrane fraction rises to 38 +/- 11% (range 18-53, n = 3). The translocation to the membrane fraction suggests that this protein may play a role in the taurine efflux in this tissue stimulated by hypotonic stress.
Assuntos
Canais de Cloreto/metabolismo , Coração/embriologia , Soluções Hipotônicas/farmacologia , Canais Iônicos , Miocárdio/metabolismo , Rajidae/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Biomarcadores , Tamanho Celular/fisiologia , Coração/efeitos dos fármacos , Técnicas In Vitro , Rajidae/embriologia , Frações Subcelulares/metabolismoRESUMO
The clearnose skate (Raja eglanteria) develops in an almost opaque eggcase and lays its eggs in pairs. One sibling from each of eight pairs of skates was removed from its eggcase during embryonic development, while the other sibling developed inside the eggcase. The refractive development of the eyes at hatching was examined to see if ambient light exposure during embryonic development could influence the refractive states of hatchlings. Measurements included refractive states, ocular dimensions and lens focal properties. The differences in measurements between the two groups were not significant, which would indicate that environmental light does not influence the refractive development of the embryonic skate eye.
Assuntos
Olho/embriologia , Refração Ocular , Rajidae/embriologia , Animais , Biometria , Embrião não Mamífero/fisiologia , Olho/anatomia & histologia , Cristalino/fisiologia , Luz SolarRESUMO
The olfactory organ of juvenile clearnose skates (Raja eglanteria) was studied with the light and electron microscopes. The organ is ovoid in shape, and its free surface is complicated by the presence of some 20 lamellae. Each lamella has a folded surface lined by a typical neurosensory olfactory epithelium. Bipolar olfactory receptor neurons, ciliated sustentacular cells, and basal cells are the pre-eminent cellular components of the epithelium. Two types of receptor neurons, both bearing microvilli but not cilia, were identified. The type 1 neuron is similar to that previously described in other fishes. The type 2 neuron has a characteristic morphology justifying a separate description. Its dendritic knob is larger than that of type 1, and its microvilli, which are shorter and thicker, are straight and regularly arranged. Tight bundles of filaments provide a skeleton to each microvillus, and these filament bundles reach more than 5 microns down into the dendrite. Type 2 receptor neurons have a well-developed Golgi complex and sparse rough endoplasmic reticulum (rER), whereas type 1 receptor neurons have a less well-developed Golgi complex and a conspicuous system of rER lamellae. The mucous layer on the epithelial surface is provided by the secretion of goblet cells that are situated mostly in the peripheral regions of each lamella. Secretory granules in the sustentacular cells and glands in the lamina propria were not observed.
Assuntos
Mucosa Olfatória/ultraestrutura , Neurônios Receptores Olfatórios/ultraestrutura , Rajidae/anatomia & histologia , Animais , Axônios/ultraestrutura , Dendritos/ultraestrutura , Epitélio/ultraestrutura , Microscopia Eletrônica , Microvilosidades/ultraestrutura , Mucosa Olfatória/embriologia , Neurônios Receptores Olfatórios/embriologia , Órgãos dos Sentidos/anatomia & histologiaRESUMO
Embryos of the clearnose skate, Raja eglanteria, develop in sea water at 20-22 degrees C, hatching after 82 +/- 4 days (Luer and Gilbert, Environ. Biol. Fishes, 13:161-171, 1985). Eyes develop as steadily enlarging spheres whose corneas have the same radius of curvature as the sclera. The cornea begins development as a 2-cell thick epithelium beneath which by Day 12 there is only a basal lamina and a wispy matrix separating it from the underlying lens. This matrix, modified by Day 16, is displaced on Day 22 by a few orthogonal plies of fibrillar primary stroma. Ply number increases to at least 13 by Day 30, reaching the final number of 20 +/- 2 by Day 42. Stromal fibroblasts (keratocytes) appear at the corneal periphery by Day 22, and in increased numbers by Day 30, a time at which no keratocytes are seen in the central stroma. However, by Day 40, many fibroblasts are present at the corneal periphery, invading the primary stroma between plies, occasionally reaching even the central cornea. By Day 53, keratocytes are present between all plies, from corneal periphery to center. Thickness of each ply in this secondary stroma increases, but the number of plies remains the same as in the primary stroma. Bowman's layer, non-invaded matrix beneath the epithelial basal lamina, is not evident until Day 53. Sutural fibers, first seen on Day 22, originate in the corneal epithelial basal lamina, traversing perpendicularly the plies of the primary stroma. Sutural fibers persist throughout development of the secondary stroma and into adulthood. In contrast to chicks, skate corneas remain transparent throughout development, and never form an endothelium.
Assuntos
Córnea/embriologia , Endotélio Corneano/embriologia , Rajidae/embriologia , Animais , Córnea/ultraestrutura , Endotélio Corneano/ultraestrutura , Células Estromais/ultraestruturaRESUMO
Morphogenesis of the clearnose skate, Raja eglanteria, was not significantly inhibited as a result of 7 days of exposure to 1-2 mM selenate in the sea water during Days 59-69 of embryonic development (hatching would normally have occurred at 82 +/- 4 days of incubation). Although corneal transparency appeared normal in the eye, preliminary measurements of the thickness of Bowman's layer of the cornea suggested that it was significantly thinner in the corneas of embryos exposed to 1-2 mM selenate. Selenate is an ion reported to inhibit sulfation of glycosaminoglycans in connective tissue.
Assuntos
Tecido Conjuntivo/efeitos dos fármacos , Córnea/embriologia , Glicosaminoglicanos/antagonistas & inibidores , Compostos de Selênio/farmacologia , Rajidae/embriologia , Animais , Tecido Conjuntivo/embriologia , Tecido Conjuntivo/metabolismo , Córnea/efeitos dos fármacos , Córnea/ultraestrutura , Relação Dose-Resposta a Droga , Embrião não Mamífero/efeitos dos fármacos , Feminino , Glicosaminoglicanos/metabolismo , Morfogênese/efeitos dos fármacos , Ácido Selênico , Fatores de TempoRESUMO
Morphogenesis of the clearnose skate, Raja eglanteria, was not significantly inhibited as a result of 7 days of exposure to 1-2 mM selenate in the sea water during Days 59-69 of embryonic development (hatching would normally have occurred at 82 +/- 4 days of incubation). Although corneal transparency appeared normal in the eye, preliminary measurements of the thickness of Bowman's layer of the cornea suggested that it was significantly thinner in the corneas of embryos exposed to 1-2 mM selenate. Selenate is an ion reported to inhibit sulfation of glycosaminoglycans in connective tissue.
Assuntos
Tecido Conjuntivo/anormalidades , Córnea/anormalidades , Compostos de Selênio/efeitos adversos , Rajidae/embriologia , Animais , Córnea/ultraestrutura , Embrião não Mamífero/anormalidades , Embrião não Mamífero/efeitos dos fármacos , Ácido SelênicoRESUMO
1. A 14.5 kDa fatty acid binding protein was isolated from the liver of the nurse shark, Ginglymostoma cirratum. 2. Purified shark liver FABP (pI = 5.4) bound oleic acid at a single site with an affinity similar to that of mammalian FABP. 3. The apparent size, pI and amino acid composition of shark liver FABP indicate a close structural relationship between this protein and mammalian heart FABP.
Assuntos
Proteínas de Transporte/isolamento & purificação , Ácidos Graxos , Fígado/química , Proteínas de Neoplasias , Proteínas do Tecido Nervoso , Ratos Endogâmicos/metabolismo , Tubarões/metabolismo , Animais , Proteínas de Transporte/química , Cromatografia em Gel , Citosol/química , Dextranos/metabolismo , Eletroforese em Gel de Poliacrilamida , Proteína 7 de Ligação a Ácidos Graxos , Proteínas de Ligação a Ácido Graxo , RatosRESUMO
The shape and focal properties (spherical aberration) of the ocular lens of hatchling, yearling and adult clearnose skates (Raja eglanteria) were examined. In contrast to the spherical shape of the typical teleost lens, the lens of the clearnose skate is slightly aspherical; equatorial diameter being greater than axial diameter. The asphericity is approximately equal, in absolute measures, at all ages and is thus more obvious in younger skates. Lens spherical aberration is well corrected at all three levels of development. Yearling and adult lenses have relative focal lengths (Matthiessen's Ratio) which are close to the representative value of 2.55:1 expected for the teleost lens. However, the relative focal length of lenses from hatchling lenses is higher (about 2.75:1). This value approaches the upper limit of reports dealing with teleost lens focal characteristics. The difference between hatchling lenses and those of adult and yearling lenses may be due to the absence of extensive compression of central lens tissue by peripheral growth at early stages in development.
Assuntos
Cristalino/crescimento & desenvolvimento , Rajidae/fisiologia , Animais , Antropometria , Cristalino/anatomia & histologia , Acuidade VisualRESUMO
1. Liver postmitochondrial supernatant preparations of calf, clearnose skate, and nurse shark were able to metabolize the fungal toxin aflatoxin B1 to various metabolites. 2. Calf liver produced aflatoxin M1 and Q1 as the major chloroform soluble metabolites, with small amounts of aflatoxicol formed during incubation. 3. Liver preparations of the elasmobranchs, however, produced aflatoxicol as the major chloroform soluble metabolite with no other metabolite being detected. 4. The water soluble metabolite profiles for the three species were also quite different with the tris diol adduct being produced to a much greater extent in calf liver preparations. 5. Aflatoxicol production by the elasmobranch liver homogenates was reversible with the skate reconverting a large amount (30%) of aflatoxicol to AFB1. The nurse shark, however, appeared to convert a portion of aflatoxicol to an unknown metabolite more polar than AFB1. 6. Calf liver DNA bound approximately 3 x more 3H-AFB1 than shark liver DNA.
Assuntos
Aflatoxinas/metabolismo , Carcinógenos/metabolismo , Bovinos/metabolismo , Peixe Elétrico/metabolismo , Fígado/metabolismo , Tubarões/metabolismo , Rajidae/metabolismo , Aflatoxina B1 , Animais , DNA/metabolismo , Técnicas In Vitro , Oxigenases de Função Mista/metabolismo , Especificidade da EspécieRESUMO
Liver microsome preparations of the elasmobranchs Ginglymostoma cirratum (nurse shark) and Raja eglanteria (clearnose skate) were examined for monooxygenase activity using aflatoxin B1 as substrate. At equiprotein concentrations, elasmobranch microsomes were less than 20% as active as calf liver in producing mutagenic metabolites of aflatoxin B1.
Assuntos
Aflatoxinas/farmacologia , Carcinógenos/farmacologia , Microssomos Hepáticos/enzimologia , Oxigenases de Função Mista/metabolismo , Aflatoxina B1 , Aflatoxinas/metabolismo , Animais , Biotransformação , Bovinos , Peixes , Histidina/farmacologia , Microssomos Hepáticos/efeitos dos fármacos , Testes de Mutagenicidade , Mutação , Salmonella typhimurium/efeitos dos fármacos , Tubarões , Especificidade da EspécieRESUMO
The effects of pH, temperature, and guanidinium chloride on the conformation of ribosomal protein L7/L12 have been investigated in order to understand the stability of this protein dimer. The results indicate that many of the molecular forces stabilizing the conformation of the dimer are disrupted at low pH or high temperature. These acid- and thermal-denatured states, however, still retain considerable secondary structure. Approximately half of the alpha-helical content present in the native protein remains intact at pH below 2 and at temperatures above 90 degrees C. Further denaturation of the acid-denatured protein by 6 M guanidinium chloride results in a state which still contains approximately 20% alpha helix. Similar amounts of residual conformation remain when the native L7/L12 dimer is denatured with guanidinium chloride. Thermodynamic analysis of the conformational transitions studied indicates that none is compatible with a simple two-state process. The complexity of these denaturation data and the structural characterizations of the various denatured states are consistent with the possible existence of structural domains in the protein molecule possessing different conformational stabilities.
Assuntos
Escherichia coli/análise , Proteínas Ribossômicas , Estabilidade de Medicamentos , Guanidinas , Concentração de Íons de Hidrogênio , Conformação Proteica , Desnaturação Proteica , Proteínas Ribossômicas/isolamento & purificação , Ribossomos/análise , Temperatura , TermodinâmicaRESUMO
The conformation of Escherichia coli ribosomal protein L7/L12 in solution has been studied using spectroscopic and hydrodynamic methods. Circular dichroism studies in the near-ultraviolet region reveal two bands at 262 and 268 nm originating from the tertiary conformational environment of the phenylalanyl residues. Additional characterization of the phenylalanine environment includes an intrinsic fluorescence emission spectrum arising from the phenylalanine fluorophores. Computer analysis of the far-ultraviolet circular dichroism spectrum suggests that L7/L12 contains as much as approximately 76% alpha helix. Hydrodynamic properties of L7/L12, measured with the purpose of providing relevant shape information, include the frictional coefficient ratio (1.84 +/- 0.03) and intrinsic viscosity (28 +/- 0.4 mL/g). The experimentally determined frictional coefficient (6.15 +/- 0.15 X 10(-8) has been compared with theoretical calculations of the same value employing two independent methods and assuming various dimensions for the L7/L12 dimer. Combining the experimental results from this work with those available from the literature, and using conformation predictive methods of Chou & Fasman [P. Y. Chou & G. D. Fasman (1974) Biochemistry 13, 211-222, 222-245] and of Maxfield & Scheraga (F. R. Maxfield & H. A. Scheraga (1976) Biochemistry 15, 5138-5153), several possible molecular models of the L7/L12 dimer have been constructed and critically examined. A model which is consistent with all of the available data is proposed.
Assuntos
Escherichia coli/análise , Proteínas Ribossômicas , Fracionamento Celular , Dicroísmo Circular , Substâncias Macromoleculares , Modelos Moleculares , Peso Molecular , Conformação Proteica , Proteínas Ribossômicas/isolamento & purificação , Ribossomos/ultraestrutura , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , ViscosidadeRESUMO
The effects of pH and temperature on the structure of human erythrocyte membranes were studied by circular dichroism (CD). The results obtained demonstrate that the membrane CD spectra undergo significant changes when the pH of the solution deviates from its native pH range of 7 to 8. Spectral changes in the acidic pH region include drastic reductions and slight shifts in the CD signal which may reflect a decrease in alpha-helical content of the proteins and/or an increase in optical artifacts, both of which are irreversible. In the alkaline pH region, dramatic increases in ellipticity and blue-shifts in the spectra are observed between pH 8 and 10. In addition, the spectra more closely resemble those reported for membrane samples where the spectral distortions have been removed. The changes in the alkaline region are demonstrated to be only partially reversible and may be due to conformational alterations in the membrane proteins and/or to a reduction in optical distortions. Thermal stability studies reinforce the irreversible behavior of the membrane samples.