Toxigenic Vibrio cholerae identified in estuaries of Tanzania using PCR techniques.

The current study assessed the occurrence of the Vibrio cholerae serogroups O1 and O139 in environmental samples along salinity gradients in three selected estuaries of Tanzania both through culture independent methods and by cultured bacteria. Occurrence of V. cholerae was determined by PCR targeting the V. cholerae outer membrane protein gene ompW. Furthermore, the presence of toxigenic strains and serogroups O1 and O139 was determined using multiplex PCR with specific primers targeting the cholera toxin gene subunit A, ctxA, and serotype specific primers, O1-rfb and O139-rfb, respectively. Results showed that V. cholerae occurred in approximately 10% (n = 185) of both the environmental samples and isolated bacteria. Eight of the bacteria isolates (n = 43) were confirmed as serogroup O1 while one belonged to serogroup O139, the first reported identification of this epidemic strain in East African coastal waters. All samples identified as serogroup O1 or O139 and a number of non-O1/O139 strains were ctxA positive. This study provides in situ evidence of the presence of pathogenic V. cholerae O1 and O139 and a number of V. cholerae non-O1/O139 that carry the cholera toxin gene in estuaries along the coast of Tanzania.

Effect of nutrient enrichment on seagrass associated meiofauna in Tanzania.

Abundance, diversity and community structure of meiofauna, with special emphasis on epiphytic harpacticoid copepods, occurring in Tanzanian seagrass beds under various nutrient inputs was determined. All measured parameters for epiphytic meiofauna and diatoms (fucoxanthin) were negatively affected by nutrient input and this was detected even at the higher taxonomic levels of meiofauna, supporting the validity of higher taxon surrogacy in environmental impact studies. However, benthic meiofauna and other biofilm characteristics (chlorophyll a) did not show any difference between sites suggesting that nutrient enrichment had less impact on these variables. This indicates a differential impact of pollution on epiphytic vs. benthic communities. Consequently, different trophic levels will be impacted in various ways and hence the effects of pollution on the overall ecosystem functioning of seagrass beds are complex and not straightforward. Although the seagrass plants themselves don't show any major changes under different nutrient input, associated organisms that guarantee energy flow at basal levels of the food web in this ecosystem can be largely impacted.

Variability in benthic diazotrophy and cyanobacterial diversity in a tropical intertidal lagoon.

Benthic nitrogen fixation has been estimated to contribute 15 Tg N year(-1) to the marine nitrogen budget. With benthic marine nitrogen fixation being largely overlooked in more recent surveys, a refocus on benthic diazotrophy was considered important. Variations in nitrogenase activity (acetylene reduction-gas chromatography) in a tropical lagoon in the western Indian Ocean (Zanzibar, Tanzania) were monitored over a 3-year period (2003-2005) and related to cyanobacterial and diazotrophic microbial diversity using a polyphasic approach. Different nitrogenase activity patterns were discerned, with the predominant pattern being high daytime activities combined with low nighttime activities. Analyses of the morphological and 16S rRNA gene diversity among cyanobacteria revealed filamentous nonheterocystous (Oscillatoriales) and unicellular (Chroococcales) representatives to be predominant. Analyses of the nifH gene diversity showed that the major phylotypes belonged to noncyanobacterial prokaryotes. However, as shown by cyanobacterial selective nifH-denaturing gradient gel electrophoresis analysis, cyanobacterial nifH gene sequences were present at all sites. Several nifH and 16S rRNA gene phylotypes were related to uncultured cyanobacteria or bacteria of geographically distant habitats, stressing the widespread occurrence of still poorly characterized microorganisms in tropical benthic marine communities.