Cannabinol Regulates the Expression of Cell Cycle-Associated Genes in Motor Neuron-like NSC-34: A Transcriptomic Analysis.

Cannabinoids are reported to have neuroprotective properties and play a role in neurogenesis and neuroplasticity in in vitro and in vivo models. Cannabinol (CBN) is a minor cannabinoid produced by the degradation of Δ9-tetrahydrocannabinol in Cannabis sativa L. and exhibits anti-oxidant, analgesic, anti-bacterial, and anti-inflammatory effects. In this study, we explored the biological effects of 20 µM CBN (6.20 µg/mL) on differentiated NSC-34 cells by MTT assay and next-generation sequencing analysis on the transcriptome. KEGG and Gene Ontology enrichment analyses have been performed to evaluate potential CBN-associated processes. Our results highlighted the absence of any cytotoxic effect of CBN. The comparative transcriptomic analysis pointed out the downregulation of Cdkn2a, Cdkn2c and Cdkn2d genes, which are known to suppress the cell cycle. Ccne2, Cdk2, Cdk7, Anapc11, Anapc10, Cdc23, Cdc16, Anapc4, Cdc27, Stag1, Smc3, Smc1a, Nipbl, Pds5a, Pds5b, and Wapl genes, renowned for their role as cell cycle progression activators, were instead upregulated. Our work suggests that CBN regulates the expression of many genes related to the cell cycle, which are required for axonal maturation, migration, and synaptic plasticity, while not affecting the expression of genes involved in cell death or tumorigenesis.

In Silico Analysis Highlights Potential Predictive Indicators Associated with Secondary Progressive Multiple Sclerosis.

Multiple sclerosis (MS) is a complex inflammatory disease affecting the central nervous system. Most commonly, it begins with recurrent symptoms followed by partial or complete recovery, known as relapsing-remitting MS (RRMS). Over time, many RRMS patients progress to secondary progressive MS (SPMS), marked by gradual symptom deterioration. The factors triggering this transition remain unknown, lacking predictive biomarkers. This study aims to identify blood biomarkers specific to SPMS. We analyzed six datasets of SPMS and RRMS patients' blood and brain tissues, and compared the differential expressed genes (DEGs) obtained to highlight DEGs reflecting alterations occurring in both brain and blood tissues and the potential biological processes involved. We observed a total of 38 DEGs up-regulated in both blood and brain tissues, and their interaction network was evaluated through network analysis. Among the aforementioned DEGs, 21 may be directly involved with SPMS transition. Further, we highlighted three biological processes, including the calcineurin-NFAT pathway, related to this transition. The investigated DEGs may serve as a promising means to monitor the transition from RRMS to SPMS, which is still elusive. Given that they can also be sourced from blood samples, this approach could offer a relatively rapid and convenient method for monitoring MS and facilitating expedited assessments.

Network Analysis Performed on Transcriptomes of Parkinson's Disease Patients Reveals Dysfunction in Protein Translation.

Parkinson's disease (PD) is a prevalent neurodegenerative disorder characterized by the progressive degeneration of dopaminergic neurons in the substantia nigra region of the brain. The hallmark pathological feature of PD is the accumulation of misfolded proteins, leading to the formation of intracellular aggregates known as Lewy bodies. Recent data evidenced how disruptions in protein synthesis, folding, and degradation are events commonly observed in PD and may provide information on the molecular background behind its etiopathogenesis. In the present study, we used a publicly available transcriptomic microarray dataset of peripheral blood of PD patients and healthy controls (GSE6613) to investigate the potential dysregulation of elements involved in proteostasis-related processes at the transcriptomic level. Our bioinformatics analysis revealed 375 differentially expressed genes (DEGs), of which 281 were down-regulated and 94 were up-regulated. Network analysis performed on the observed DEGs highlighted a cluster of 36 elements mainly involved in the protein synthesis processes. Different enriched ontologies were related to translation initiation and regulation, ribosome structure, and ribosome components nuclear export. Overall, this data consistently points to a generalized impairment of the translational machinery and proteostasis. Dysregulation of these mechanics has been associated with PD pathogenesis. Understanding the precise regulation of such processes may shed light on the molecular mechanisms of PD and provide potential data for early diagnosis.

Positive impact of a functional ingredient on hunger and satiety after ingestion of two meals with different characteristics.

The ingestion of unavailable carbohydrates - functional ingredients - has presented an inverse relationship with the risk for chronic non-communicable diseases. The objective of this work was to evaluate the effects of addition of inulin to two ready-to-eat frozen meals on the release of gastrointestinal hormones and other parameters related to hunger and satiety. Prototypes of two different kinds of frozen meals were elaborated by the food industry: control meal (C1 and C2); and test meals, added inulin (T1 and T2). Three sequential clinical assays were performed with healthy volunteers: 1) evaluation of glycemic response (n=16); 2) evaluation of gastrointestinal hormones related to satiety (n=15); and 3) evaluation of satiety (by Visual Analogue Scale - VAS and energy intake) (n=52). The meals showed low glycemic index and glycemic load, and T1 showed a decreased glycemic response peak compared to C1. The addition of inulin (~8g) to the test meals (lunch) provided significant satiety, resulting in an decrease in energy intake of 419 (group 1) and 586kJ (group 2) in the two subsequent meals (after 180min and 360min) and a decrease in hunger and increase in satiety at 120 and 180min when comparing with control meals. A positive post-prandial variation was observed in the plasmatic levels of ghrelin and insulin in relation to the control meal (hormones related to hunger in high levels), after the intake of both two test meals. Inulin is an ingredient that presents several positive characteristics for the elaboration of products that stimulate healthy eating habits. These effects are currently being evaluated in medium-term trials.

Campaña de atención dermatológica en el interior de la provincia de Jujuy: nuestra experiencia / Dermatologic assistance campaing throughout Jujuy: our experience

La Sociedad Jujeña de Dermatología, estimulada por la Campaña de Prevención del Cáncer de Piel que organiza la SAD y en adhesión a la misma, cumple anualmente actividades de atención dermatológica en el interior de la provincia de Jujuy, priorizando la misma en los lugares más críticos en cuanto a asistencia especializada y acceso geográfico. Presentamos un informe de la última Campaña, brindando aspectos históricos y antecedentes y un resúmen de la patología relevante, destacando las características étnicas y regionales como factor de incidencia de la misma, así como la ausencia de cáncer de piel

Influência da assistência de enfermagem na profilaxia da hiperêmese gravídica: estudo piloto / Influence of nursing care in the prevention of hyperemesis gravidarum

A etiologia da hiperêmese gravídica é desconhecida, porém, vários autores consideram a origem psíquica coma a mais provável. Neste trabalho, estudou-se a influência da assistência de enfermagem às gestantes com náuseas e vômitos matinais ou vespertinos, na profilaxia da hiperêmese gravídica. A assistência de enfermagem consistiu basicamente em orientaçäo sobre alimentaçäo e ajuda na resoluçäo de problemas emocionais.

Análise da Revista da Escola de Enfermagem da USP: período de 1967 a 1981 / Analysis of Revista da Escola de Enfermagen da Universidade de Säo Paulo: period of 1967 to 1981

É apresentada a análise do contéudo da Revista da Escola de Enfermagem da USP no período de 1967 a 1981, quanto a: tipo de material publicado, seus temas, bem como, campo de atuaçäo e grau acadêmico dos autores

[Contents of Revista da Escola de Enfermagem da USP: period of 1967 to 1981]. / ANÁLISE DA REVISTA DA ESCOLA DE ENFERMAGEM DA USP: Período de 1967 a 1981.

The contents of the first fifteen volumes of Revista da Escola de Enfermagem da USP, are viewed as to type of matter published, their, themes, as well as the author's work field and academic degrees.

Assistência de enfermagem à mulher no ciclo grávido-puerperal: aspecto psicossocial / Nursing care to the woman in the gravidopuerperal period: psychosocial aspect

As autoras relatam os componentes da enfermagem obstétrica - a mulher no ciclo grávido-puerperal, o problema existente, o contexto onde a mulher é assistida e a assistência de enfermagem que lhe é prestada. É dada ênfase ao relacionamento terapêutico enfermeira-paciente. Säo apresentados os instrumentos de apoio da assistência de enfermagem: observaçäo, entrevista, visita domiciliária e o registro destes. Para assegurar o bem-estar do binômio mäe-filho säo descritas as funçöes: encaminhamento, relacionamento terapêutico enfermeira-paciente e intervençäo em crise.

[Nursing care to the woman in the perinatal period - psychosocial aspect]. / ASSISTÊNCIA DE ENFERMAGEM À MULHER NO CICLO GRÁVIDO-PUERPERAL ­ ASPECTO PSICOSSOCIAL.

The authors describe the components of obstetrical nursing as the woman in the perinatal period, the existent problem, the context where the woman is assisted and the nursing care that is given to her. The emphasis is placed in the therapeutic nurse-patient relationship. The authors present instruments to support nursing care as observation, interviews, domiciliary visit, and the record of these three itens. To assure, as far as possible, the well-being of the binomial mother-child the nurse has to develope functions as guidance, therapeutic nurse-patient relationship, and crisis intervention.

[Instrument to analyse periodicals of nursing]. / INSTRUMENTO PARA ANÁLISE DE PERIÓDICO DE ENFERMAGEM.

Studies about spreading of knowledge in nursing are recent. In order to contribute to the study of the nursing literature in Brazil, it was developed a model of instrument to analyse periodicals. The instrument was tested in a nursing periodical and it was found to be effective in stablishing who writes, what is written and even to relate the papers with present state of health and the historical evolution of the profession.

[Influence of nursing care in the prevention of hyperemesis gravidarum]. / INFLUÊNCIA DA ASSISTÊNCIA DE ENFERMAGEM NA PROFILAXIA DA HIPERÊMESE GRAVÍDICA: ESTUDO PILOTO.

The objectives of this study was to find out if nursing assistance given to pregnant women, specifically in relation to nauses and vomiting, would help prevent hyperemesis gravidarum. Nursing assistance consisted basically in orientation regarding diet and help in the solution of emotional problems, both given to the palíenles when they came to the out patient department for pre-natal control. Thirty pregnant women were studied. The data obtained were not significant, due probabity to a number of causes, including: small population; infrequent nurse-patient meetings; lack of control over the patients' diet. This study was therefore considered as a pilot study.