[Arginine and lysine as products of basic carboxypeptidase activity associated with fibrinolysis].

Blood carboxypeptidases play an important role in the regulation of fibrinolysis. We have proposed here the method for the assay of blood carboxypeptidase activity associated with coagulation/fibrinolysis using the natural substrate fibrin and the detection of basic amino acids arginine and lysine as products in the conditions close to those in vivo. Plasma samples from 15 patients with arterial hypertension were investigated. The coagulation and subsequent fibrinolysis were initiated by addition of standard doses of thrombin and tissue plasminogen activator, respectively. Arginine and lysine concentrations before, during, and after completion of fibrinolysis were determined using HPLC. The parameters of fibrinolysis were evaluated by clot turbidity assay. Fibrinolysis led to a large and significant increase in concentrations of arginine and lysine in the incubation mixture by 101 and 81%, respectively. The duration of fibrinolysis initiation significantly correlated to the degree of increase of these amino acids: r(s) = -0.733 and -0.761 for arginine and lysine, respectively (p < 0.05). The rates of amino acids liberation during fibrinolysis demonstrate different pattern: arginine generation had two maximums: at the beginning of clot lysis and at his end, whereas the liberation of lysine occurred mainly at the middle of fibrinolysis. Thus, the carboxypeptidase activity associated with fibrinolysis can be considered as a local source of the essential aminoacids.

[The treatment of herpetic keratitis by ion-plasma coagulation of the cornea]. / Tratamentul keratitei herpetice prin coagularea ionoplasmica a corneei.

The authors propose a new treatment method for herpetic keratitis: ion-plasmic corneal coagulation using a "Strimer" device designed and build by themselves. After successfully experimenting the action of the ion-plasmic flow on the guinea pigs cornea, the "Strimer" device has been used in the clinic. Using this device, 32 patients with herpetic keratitis have been treated, 3 of them having metaherpetical keratitis. Most of the patients (25) were cured and left the hospital after 4-7 days from intervention, with the visual acuity improved from 0.1-0.5 to 0.7-1. The others 7 patients left the hospital after 10-15 days from the intervention, with a better visual acuity.