Root exudates facilitate the regulation of soil microbial community function in the genus Haloxylon.

Introduction: Root exudates act as the "language" of plant-soil communication, facilitating crucial interactions, information exchange, and energy transfer between plants and soil. The interactions facilitated by root exudates between plants and microorganisms in the rhizosphere are crucial for nutrient uptake and stress resilience in plants. However, the mechanism underlying the interaction between root exudates and rhizosphere microorganisms in desert plants under drought conditions remains unclear, especially among closely related species. Methods: To reveal the ecological strategies employed by the genus Haloxylon in different habitats. Using DNA extraction and sequencing and UPLC-Q-Tof/MS methods, we studied root exudates and soil microorganisms from two closely related species, Haloxylon ammodendron (HA) and Haloxylon persicum (HP), to assess differences in their root exudates, soil microbial composition, and interactions. Results: Significant differences were found in soil properties and root traits between the two species, among which soil water content (SWC) and soil organic carbon (SOC) in rhizosphere and bulk soils (P < 0.05). While the metabolite classification of root exudates was similar, their components varied, with terpenoids being the main differential metabolites. Soil microbial structure and diversity also exhibited significant differences, with distinct key species in the network and differential functional processes mainly related to nitrogen and carbon cycles. Strong correlations were observed between root exudate-mediated root traits, soil microorganisms, and soil properties, although the complex interactions differed between the two closely relative species. The primary metabolites found in the network of HA include sugars and fatty acids, while HP relies on secondary metabolites, steroids and terpenoids. Discussion: These findings suggest that root exudates are key in shaping rhizosphere microbial communities, increasing microbial functionality, fostering symbiotic relationships with hosts, and bolstering the resilience of plants to environmental stress.

Regulation of drought stress on nutrient cycle and metabolism of rhizosphere microorganisms in desert riparian forest.

Microbial communities in desert riparian forest ecosystems have developed unique adaptive strategies to thrive in harsh habitats shaped by prolonged exposure to abiotic stressors. However, the influence of drought stress on the functional and metabolic characteristics of soil rhizosphere microorganisms remains unknown. Therefore, this study aimed to investigate the effects of drought stress on soil biogeochemistry and metabolism and analyze the relationship between the biogeochemical cycle processes and network of differentially-expressed metabolites. Using metagenomics and metabolomics, this study explored the microbial functional cycle and differential metabolic pathways within desert riparian forests. The predominant biogeochemical cycles in the study area were the Carbon and Nitrogen cycles, comprising 78.90 % of C, N, Phosphorus, Sulfur and Iron cycles. Drought led to increased soil C fixation, reduced C degradation and methane metabolism, weakened denitrification, and decreased N fixation. Furthermore, drought can disrupt iron homeostasis and reduce its absorption. The differential metabolic pathways of drought stress include flavonoid biosynthesis, arachidonic acid metabolism, steroid hormone biosynthesis, and starch and sucrose degradation. Network analysis of functional genes and metabolism revealed a pronounced competitive relationship between the C cycle and metabolic network, whereas the Fe cycle and metabolic network promoted each other, optimizing resource utilization. Partial least squares analysis revealed that drought hindered the expression and metabolic processes and functional genes, whereas the rhizosphere environment facilitated metabolic expression and the functional genes. The rhizosphere effect primarily promoted metabolic processes indirectly through soil enzyme activities. The integrated multi-omics analysis further revealed that the effects of drought and the rhizosphere play a predominant role in shaping soil functional potential and the accumulation of metabolites. These insights deepen our comprehension of desert riparian forest ecosystems and offer strong support for the functionality of nutrient cycling and metabolite dynamics.

Organic Photoredox-Catalyzed Site-Selective Alkylation of Glycine Derivatives and Peptides via Infrequent 1,2-Hydrogen Atom Transfer of Amidyl Radicals.

Herein, we disclose a visible-light-driven photoredox-catalyzed protocol for site-selective alkylation of glycine derivatives via 1,2-hydrogen atom transfer, which is distinguished by metal free and mild conditions, high chemoselectivity, and good functional group compatibility. This protocol provides a unique approach for synthesizing valuable α,ß-diamino acid derivatives. Furthermore, the potential synthetic merit of this transformation is proven by a scale-up reaction and late-stage functionalization of peptides.

B(C6F5)3·H2O-Catalyzed N-H and C-H Functionalization of Aromatic Amines with Sulfoxonium Ylides under Metal-Free Conditions.

Hydrogen-bonded aggregates of B(C6F5)3 and water are proven as strong Brønsted acid catalysts, which have the advantages of low toxicity and broad compatibility. Sulfoxonium ylides are stable surrogates of diazo compounds, which participate in various reactions due to their versatile reactivity. Based on these characteristics, a strategy for C-N bond or C-C bond construction of aromatic amines with sulfoxonium ylides under metal-free catalytic conditions was established. This method has advantages of mild conditions and excellent yield, which is suitable for the N-H or C-H functionalization of various aromatic amine compounds.

Visible-light-induced C-H alkylation of 2-amino-1,4-naphthoquinones.

Simple and practical strategies for visible-light-induced C-H alkylation of 2-amino-1,4-naphthoquinones with cyclobutanone oxime esters and hydroxamic acid derivatives have been developed under mild and redox-neutral conditions. These two reactions can be carried out at room temperature and obtain a variety of 2-amino-1,4-naphthoquinone derivatives with cyano and amide groups. Moreover, the cyanoalkylation reaction of 2-amino-1,4-naphthoquinones can proceed smoothly in the absence of photocatalysts.

Visible-Light-Driven Coupling of 1,3,4-Oxadiazoles and Hydroxamic Acid Derivatives.

A visible-light-induced radical-radical cross-coupling reaction between 1,3,4-oxadiazoles and hydroxamic acid derivatives has been realized under base- and metal-free conditions. The protocol was characterized by broad substrate scope, excellent functional group tolerance, and simple operation procedures. By using this protocol, a variety of biologically important 5-aryl-1,3,4-oxadiazole-2-methylamines were obtained in good yields with excellent chemoselectivity.

Synthesis of Nonclassical Heteroaryl C-Glycosides via Decarboxylative C-H Glycosylation.

A photoredox-promoted decarboxylative C-H glycosylation for the synthesis of nonclassical heteroaryl C-glycosides is reported. This methodology is characterized by an exceedingly simple reaction system, high diastereoselectivity, and good functional group tolerance. Moreover, the operational procedure is simple, and the gram-scale reaction highlights the practical applicability of this protocol.

Visible-light-induced synthesis of heteroaryl C-glycosides via decarboxylative C-H glycosylation.

A photoredox promoted decarboxylative C-H glycosylation has been developed for the synthesis of heteroaryl C-glycosides. This methodology is characterized by its exceedingly simple reaction system, high diastereoselectivity and good functional group tolerance. Moreover, this innovative approach circumvents the need for high temperatures, transition metals, and photocatalysts, providing an environmentally friendly, straightforward, and efficient protocol.

Impact of meteorological variability on diurnal and seasonal net ecosystem productivity in a desert riparian forest ecosystem.

The desert riparian forests are susceptible to meteorological changes and contribute significantly to the net ecosystem productivity (NEP) variations of arid ecosystems. However, the responsive patterns of their NEP variations to the meteorological variabilities remain inadequately comprehended. To address this gap, we utilized seven years of eddy covariance flux measurements in a representative desert riparian forest to investigate the NEP variations and its response to changing meteorological factors across diverse temporal scales. The results revealed significant periodic variations in half-hourly NEP, with dominant cycles spanning from five hours to one year, with a principal oscillation period of one day. Key meteorological factors including global solar radiation (Rg), relative humidity (RH), air temperature (Ta), soil temperature (Ts), and vapor pressure deficit (VPD) exhibited synchronization with NEP on daily scales. This synchronization, coupled with the observed one-day periodic NEP variations, provides robust evidence supporting the existence of a circadian rhythm in the ecosystem carbon exchange of desert riparian forest regulated by meteorological conditions. Seasonal patterns were significant in the impact of Rg phase, Ta diurnal amplitude, and VPD diurnal amplitude on NEP diurnal amplitude and phase. The NEP diurnal amplitude significantly, directly, and positively affected daily NEP in both the dormant and growing seasons, whereas its phase yielded significant negative effects (P< 0.05). The averages, amplitudes, and phases of diurnal meteorological conditions controlled the daily NEP by regulating NEP diurnal amplitude and phase. These findings provide evidence that the variability in circadian rhythms, caused by the increase in diurnal Ta and VPD, significantly impact the daily NEP at an ecosystem scale. This study enriches our comprehension of the meteorological mechanisms governing diurnal and seasonal carbon uptake dynamics within desert riparian forests, providing fresh insights into the direct and indirect roles of climate change in shaping patterns of ecosystem carbon exchange.

Effects of Root-Root Interactions on the Physiological Characteristics of Haloxylon ammodendron Seedlings.

The root traits and response strategies of plants play crucial roles in mediating interactions between plant root systems. Current research on the role of root exudates as underground chemical signals mediating these interactions has focused mainly on crops, with less attention given to desert plants in arid regions. In this study, we focused on the typical desert plant Haloxylon ammodendron and conducted a pot experiment using three root isolation methods (plastic film separation, nylon mesh separation, and no separation). We found that (1) as the degree of isolation increased, plant biomass significantly increased (p < 0.05), while root organic carbon content exhibited the opposite trend; (2) soil electrical conductivity (EC), soil total nitrogen (STN), soil total phosphorus (STP), and soil organic carbon (SOC) were significantly greater in the plastic film and nylon mesh separation treatments than in the no separation treatment (p < 0.05), and the abundance of Proteobacteria and Actinobacteriota was significantly greater in the plastic film separation treatment than in the no separation treatment (p < 0.05); (3) both plastic film and nylon mesh separations increased the secretion of alkaloids derived from tryptophan and phenylalanine in the plant root system compared with that in the no separation treatment; and (4) Pseudomonas, Proteobacteria, sesquiterpenes, triterpenes, and coumarins showed positive correlations, while both pseudomonas and proteobacteria were significantly positively correlated with soil EC, STN, STP, and SOC (p < 0.05). Aurachin D was negatively correlated with Gemmatimonadota and Proteobacteria, and both were significantly correlated with soil pH, EC, STN, STP, and SOC. The present study revealed strong negative interactions between the root systems of H. ammodendron seedlings, in which sesquiterpenoids, triterpenoids, coumarins, and alkaloids released by the roots played an important role in the subterranean competitive relationship. This study provides a deeper understanding of intraspecific interactions in the desert plant H. ammodendron and offers some guidance for future cultivation of this species in the northwestern region of China.

Nitraria sibirica adapts to long-term soil water deficit by reducing photosynthesis, stimulating antioxidant systems, and accumulating osmoregulators.

Amid climate change and shifts in precipitation patterns, drought conditions are expanding worldwide. Drought stress severely threatens plant growth in arid and semi-arid regions, wherein shrubs play a crucial role in maintaining ecological stability. Despite its ecological significance, studies are lacking on how Nitraria sibirica adapts to long-term drought stress. Therefore, in this study, to elucidate the mechanism of drought stress adaptation in N. sibirica, we analysed morphological, physiological, and transcriptional characteristics of plants in two soil habitats: riparian (moist) and desert (arid). The results showed that in desert soils, as soil water content decreased, leaf thickness increased, while plant height and leaf area decreased. Physiologically, photosynthesis decreased; soluble sugar, starch, proline, and hydrogen peroxide content increased significantly; while soluble proteins decreased significantly. Additionally, membrane lipid peroxidation products and antioxidant enzyme activities significantly increased under drought stress. Then, Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis identified 313 key genes, which were considered the most significantly enriched in the photosynthesis and photosynthetic antenna protein pathways. Further, we found that the proteins encoding photosystem II (PsbP, PsbQ, PsbR, PsbY, and Psb27), photosystem I (PsaD, PsaF, PsaG, PsaH, PsaK, and PsaO), photosynthetic electron transport (PetF), and light-trapping antenna proteins were significantly downregulated under drought stress. Taken together, these results suggest that N. sibirica adapts to long-term drought conditions by suppressing photosynthesis, activating antioxidant systems, and recruiting osmoregulators. This study provides a basis for elucidating the growth mechanisms of N. sibirica under long-term drought stress conditions.

Visible-light-mediated synthesis of non-anomeric S-aryl glycosides via a photoactive electron-donor-acceptor complex.

A visible-light-mediated glycosylation reaction between glycosyl redox-active esters and disulfides has been reported, through which a series of S-aryl glycosides were obtained in good yields with satisfactory stereoselectivity. The preliminary mechanistic studies revealed that this transformation proceeded via an EDA complex. Moreover, the potential application value was demonstrated in the late-stage functionalisation of drug molecules and a gram-scale experiment.

Pathway to Construct α-Acyloxy Esters by B(C6F5)3-Catalyzed O-H Insertion of Carboxylic Acids with Sulfoxonium Ylides.

We report the first example of B(C6F5)3-catalyzed O-H insertion reaction of sulfoxonium ylides and carboxylic acids, achieving efficient construction of diester moieties under metal-free condition. This protocol is characterized by broad substrate tolerance, particularly for various phenylacetic acids, and good compatibility with water/air condition, which is superior to most other methods.

Differential effects of cow dung and its biochar on Populus euphratica soil phosphorus effectiveness, bacterial community diversity and functional genes for phosphorus conversion.

Introduction: Continuous monoculture leading to soil nutrient depletion may cause a decline in plantation productivity. Cow dung is typically used as a cheap renewable resource to improve soil nutrient status. In this study, our purpose was to compare the effects of different cow dung return methods (direct return and carbonization return) on soil microbial communities and phosphorus availability in the root zone (rhizosphere soil and non-rhizosphere soil) of P.euphratica seedlings in forest gardens and to explore possible chemical and microbial mechanisms. Methods: Field experiments were conducted. Two-year-old P.euphratica seedlings were planted in the soil together with 7.5 t hm-2 of cow dung and biochar made from the same amount of cow dung. Results: Our findings indicated that the available phosphorus content in soil subjected to biochar treatment was considerably greater than that directly treated with cow dung, leading to an increase in the phosphorus level of both aboveground and underground components of P.euphratica seedlings. The content of Olsen-P in rhizosphere and non-rhizosphere soil increased by 134% and 110%, respectively.This was primarily a result of the direct and indirect impact of biochar on soil characteristics. Biochar increased the biodiversity of rhizosphere and non-rhizosphere soil bacteria compared with the direct return of cow dung. The Shannon diversity index of carbonized cow manure returning to field is 1.11 times and 1.10 times of that of direct cow manure returning to field and control, and the Chao1 diversity index is 1.20 times and 1.15 times of that of direct cow manure returning to field and control.Compared to the direct addition of cow dung, the addition of biochar increased the copy number of the phosphorus functional genes phoC and pqqc in the rhizosphere soil. In the biochar treatment, the abundance of the phosphate-solubilizing bacteria Sphingomonas and Lactobacillus was significantly higher than that in the other treatments, it is relative abundance was 4.83% and 2.62%, respectively, which indirectly improved soil phosphorus availability. Discussion: The results indicated that different cow dung return methods may exert different effects on phosphorus availability in rhizosphere and non-rhizosphere soils via chemical and microbial pathways. These findings indicated that, compared to the direct return of cow dung, biochar return may exert a more significant impact on the availability of phosphorus in both rhizosphere and non-rhizosphere soils, as well as on the growth of P.euphratica seedlings and the microbial community.

Prediction of spatial distribution characteristics of ecosystem functions based on a minimum data set of functional traits of desert plants.

The relationship between plant functional traits and ecosystem function is a hot topic in current ecological research, and community-level traits based on individual plant functional traits play important roles in ecosystem function. In temperate desert ecosystems, which functional trait to use to predict ecosystem function is an important scientific question. In this study, the minimum data sets of functional traits of woody (wMDS) and herbaceous (hMDS) plants were constructed and used to predict the spatial distribution of C, N, and P cycling in ecosystems. The results showed that the wMDS included plant height, specific leaf area, leaf dry weight, leaf water content, diameter at breast height (DBH), leaf width, and leaf thickness, and the hMDS included plant height, specific leaf area, leaf fresh weight, leaf length, and leaf width. The linear regression results based on the cross-validations (FTEIW - L, FTEIA - L, FTEIW - NL, and FTEIA - NL) for the MDS and TDS (total data set) showed that the R2 (coefficients of determination) for wMDS were 0.29, 0.34, 0.75, and 0.57, respectively, and those for hMDS were 0.82, 0.75, 0.76, and 0.68, respectively, proving that the MDSs can replace the TDS in predicting ecosystem function. Then, the MDSs were used to predict the C, N, and P cycling in the ecosystem. The results showed that non-linear models RF and BPNN were able to predict the spatial distributions of C, N and P cycling, and the distributions showed inconsistent patterns between different life forms under moisture restrictions. The C, N, and P cycling showed strong spatial autocorrelation and were mainly influenced by structural factors. Based on the non-linear models, the MDSs can be used to accurately predict the C, N, and P cycling, and the predicted values of woody plant functional traits visualized by regression kriging were closer to the kriging results based on raw values. This study provides a new perspective for exploring the relationship between biodiversity and ecosystem function.

Foliar zinc reduced Cd accumulation in grains by inhibiting Cd mobility in the xylem and increasing Cd retention ability in roots1.

Cadmium (Cd) pollution endangers the safe utilization of paddy soils, and foliar zinc (Zn) can reduce the toxic effects of Cd. However, little is known about the effects of foliar Zn application on the transport and immobilization of Cd in key rice tissues and the physiological state of rice plants. A pot experiment was conducted to explore the effects of spraying 0.2% and 0.4% Zn (ZnSO4) during the early grain-filling stage on Cd transport in rice, photosynthesis, glutathione (GSH) levels, Cd concentrations in xylem sap, and the expression of Zn transporter genes. The results showed that grain Cd concentrations in the 0.2% Zn and 0.4% Zn treatments were 24% and 31% lower, respectively, than those of the control treatments at maturity. Compared with the control treatments, the 0.4% Zn treatment increased Cd by 60%, 69%, 23%, and 22% in husks, rachises, first internodes, and roots, respectively. Application of Zn reduced xylem Cd content by up to 26% and downregulated transporter genes (OSZIP12, OSZIP4, and OSZIP7a) in flag leaves. Foliar Zn increased Cd bioaccumulation in roots while decreasing Cd bioaccumulation in grains. Zn reduced GSH concentration in flag leaves and stems, inhibiting photosynthesis (intercellular CO2 concentration, transpiration rate). Taken together, foliar Zn can reduce the expression of Zn transporter genes and the mobility of Cd in the xylem, promoting the fixation of Cd in husks, rachises, first internodes, and roots, ultimately reducing Cd concentration in rice grains.

Metabolomic Analysis of the Response of Haloxylon ammodendron and Haloxylon persicum to Drought.

Haloxylon ammodendron and Haloxylon persicum, as typical desert plants in arid areas, show strong drought tolerance and environmental adaptability and are therefore ideal model plants for studying the molecular mechanisms of drought tolerance. A metabolomic analysis of H. ammodendron and H. persicum in their natural environment is lacking, and their metabolic response to drought therefore remains unclear. To elucidate the response of H. ammodendron and H. persicum to drought at the metabolic level, a non-targeted metabolomics analysis was carried out herein. Under a dry environment, H. ammodendron exhibited 296 and 252 differentially expressed metabolites (DEMs) in the positive and negative ion modes, respectively, whereas 452 and 354 DEMs were identified in the positive and negative ion modes in H. persicum, respectively. The results indicated that H. ammodendron responds to drought by increasing the content of organic nitrogen compounds and lignans, neolignans, and related compounds, and reducing the content of alkaloids and derivatives. By contrast, H. persicum adapts to the dry environment by increasing the content of organic acids and their derivatives and reducing the content of lignans, neolignans, and related compounds. In addition, H. ammodendron and H. persicum improved their osmoregulation ability, reactive oxygen species detoxification ability, and cell membrane stability by regulating the key metabolic pathways and anabolism of associated metabolites. This is the first metabolomics report on the response of H. ammodendron and H. persicum to drought in their natural environment, providing a foundation for the further study of their regulatory mechanisms under drought stress.

The Pyridotriazole Works as a Traceless Directing Group: A C-H Activation/Annulation Cascade Reaction with Iodonium Ylides.

A novel Rh-catalyzed cascade reaction of pyridotriazoles with iodonium ylides is reported. This one-pot procedure involves a triazole-directed ortho-position C-H carbene insertion, followed by intramolecular denitrogenation annulation. It was noteworthy that this reaction provided straightforward access to 1H-isochromene frameworks with excellent yields (up to 94% yield).

Response of water and photosynthetic physiological characteristics to leaf humidification in Calligonum ebinuricum.

Foliar water uptake (FWU) has increasingly been regarded as a common approach for plants to obtain water under water-limited conditions. At present, the research on FWU has mostly focused on short-term experiments; the long-term FWU plant response remains unclear; Methods: Through a field in-situ humidification control experiment, the leaves of Calligonum ebinuricum N. A. Ivanova ex Soskov were humidified, and the changes of leaf water potential, gas exchange parameters and fluorescence physiological parameters of plants after long-term and short-term FWU were discussed; The main results were as follows: (1) After short-term humidification, the water potential of Calligonum ebinuricum decreased, the non-photochemical quenching (NPQ) increased, and the plant produced photoinhibition phenomenon, indicating that short-term FWU could not alleviate drought stress. (2) After long-term humidification, the leaf water potential, chlorophyll fluorescence parameter and net photosynthetic rate (Pn) increased significantly. That is to say, after long-term FWU, the improvement of plant water status promoted the occurrence of light reaction and carbon reaction, and then increased the net photosynthetic rate (Pn); Therefore, long-term FWU is of great significance to alleviate drought stress and promote Calligonum ebinuricum growth. This study will be helpful to deepen our understanding of the drought-tolerant survival mechanism of plants in arid areas.

Structural Characteristics and Assembly Mechanisms of Soil Microbial Communities under Water-Salt Gradients in Arid Regions.

Exploring the structural characteristics of arid soil microbial communities and their assembly mechanisms is important for understanding the ecological characteristics of arid zone soils and promoting ecological restoration. In this study, we used Illumina high-throughput sequencing technology to study soils in the arid zone of the Lake Ebinur basin, determined the differences among soil microbial community structures in the study area under different water-salt gradients, and investigated the effects of environmental factors on microbial community structure and assembly mechanisms. The results show the following: the microbial community alpha diversity exhibited a significantly higher low water-salt gradient (L) than high water-salt gradient (H) and medium water-salt gradient (M). The pH was most strongly correlated with soil microbial community structure, where the alpha diversity indices of the bacterial community and fungal community were significantly negatively correlated with pH, and the Bray-Curtis distance of bacterial community was significantly positively correlated with pH (p < 0.05). The complexity of bacterial community co-occurrence networks showed a significantly higher L than H and M, and the complexity of fungal community co-occurrence network showed a significantly lower L than H and M. The cooperative relationship of H and M in the co-occurrence networks was stronger than that of the L, and the key species of the microbial co-occurrence network were different under different water-salt gradients. Stochastic processes dominated the assembly mechanism of the microbial community structure of soil, and the explanation rates of deterministic and stochastic processes were different under different water-salt gradients, with the highest explanation rate of stochastic processes on the L accounting for more than 90%. In summary, the soil microbial community structure and assembly mechanisms significantly differed across water-salt gradients, and these findings can help provide a reference for further research on soil microbiology in arid zones.