RESUMO
A simple and effective method was proposed to prepare an imprinted polymer layer at the surface of magnetic carbon dots with dopamine as the functional monomer for selectively and sensitively fluorescent recognizing bovine hemoglobin. The magnetic fluorescence imprinted polymers were investigated by Fourier-transform infrared spectra, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, and vibrating sample magnetometer. Under optimum conditions, the fluorescent intensity decreased linearly coincided with the concentration of bovine hemoglobin in the range of 0.05-16.0⯵M with a detection limit of 17.3â¯nM. The magnetic fluorescence imprinted polymers were used for detecting bovine hemoglobin in real samples with the recoveries of 99.0-104.0%. These results indicated that a convenient method was proposed to develop fluorescent probes for rapid recognition and sensitive detection of trace proteins in real samples.
Assuntos
Carbono/química , Corantes Fluorescentes/química , Fluorometria/métodos , Hemoglobinas/análise , Pontos Quânticos/química , Adsorção , Animais , Bovinos , Fluorescência , Corantes Fluorescentes/síntese química , Hemoglobinas/química , Humanos , Concentração de Íons de Hidrogênio , Indóis/química , Limite de Detecção , Fenômenos Magnéticos , Tamanho da Partícula , Polímeros/química , Reprodutibilidade dos TestesRESUMO
A novel three-dimensional (3D) magnetic imprinted polymer was prepared with bovine serum albumin as the template molecule, dopamine as the functional monomer, and a graphene oxide and carbon nanotube hybrid 3D nanocomposite as the carrier. The preparation conditions for the 3D magnetic molecularly imprinted polymers were optimized. The 3D magnetic molecularly imprinted polymers were characterized in detail by scanning electron microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometry. The 3D magnetic molecularly imprinted polymers possessed a fast adsorption rate and excellent adsorption performance toward bovine serum albumin, with a maximum adsorption capacity of 78.12 mg g-1. The extraction conditions, including the washing solvent, the pH of the eluent, and the desorption time, were also optimized. Combined with high-performance liquid chromatography, the 3D magnetic molecularly imprinted polymers were successfully applied to enrich and separate bovine serum albumin from bovine calf serum samples with recoveries of 84.0-94.5%. Graphical Abstract á .
Assuntos
Magnetismo , Impressão Molecular/métodos , Soroalbumina Bovina/química , Extração em Fase Sólida/métodos , Grafite/química , Microscopia Eletrônica de Varredura , Nanotubos de Carbono , Propriedades de SuperfícieRESUMO
A novel three-dimensional (3D) magnetic chlorogenic acid (CGA) imprinted polymer (MMIP) was prepared with novel carbon hybrid nanocomposite as the carrier, chlorogenic acid as the template molecule, and methacrylic acid as the functional monomer. The 3D MMIPs were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, vibrating sample magnetometer, and UV spectrometry in detail. The results showed that the imprinted layer was attached successfully on the surface of a 3D magnetic carbon hybrid nanocomposite. The adsorption performance of the 3D MMIPs was investigated, and the results showed that the 3D MMIPs exhibited high adsorption capacity and fast adsorption rate toward CGA with a maximum adsorption capacity of 10.88 mg g(-1). The extraction conditions involving washing solvent, the pH of eluent solvent, elution volume, and desorption time were also investigated in detail. Combined with high-performance liquid chromatography, the 3D MMIPs have been applied to successfully extract CGA from Eucommia leaf extract samples.