RESUMO
In the present study, the molecular and magnetic dual-targeted redox-responsive folic acid-cysteine-Fe3O4 microcapsules (FA-Cys-Fe3O4 MCs) have been synthesized via the sonochemical technique, and targeting molecule (folic acid) and Fe3O4 magnetic nanoparticles are introduced into the microcapsule shells successfully. The obtained FA-Cys-Fe3O4 MCs show excellent magnetic responsive ability by the oriented motion under an external magnetic field. The hydrophobic fluorescent dye (Coumarin 6) is successfully loaded into the FA-Cys-Fe3O4 MCs, demonstrating that it could be also easily realized to encapsulate hydrophobic drugs into the FA-Cys-Fe3O4 MCs when the drugs are dispersed into the oil phase before sonication. Cellular uptake demonstrates that FA-Cys-Fe3O4 MCs could target selectively the cells via folate-receptor-mediated endocytosis. Moreover, the FA-Cys-Fe3O4 MCs show their potential ability to be an attractive carrier for drug controlled release owing to the redox responsiveness of the disulfide in the microcapsule shells.
Assuntos
Cápsulas/síntese química , Preparações de Ação Retardada/síntese química , Ácido Fólico/administração & dosagem , Nanopartículas de Magnetita/química , Sonicação/métodos , Cápsulas/efeitos da radiação , Cisteína/química , Difusão , Composição de Medicamentos/métodos , Ácido Fólico/química , Células HeLa , Ondas de Choque de Alta Energia , Humanos , Nanopartículas de Magnetita/administração & dosagem , Nanopartículas de Magnetita/ultraestrutura , Teste de Materiais , Oxirredução/efeitos da radiação , Tamanho da PartículaRESUMO
We designed a biocompatible carrier for controlled release of hydrophobic drugs. The designed carrier was prepared by sonicating oil in a protein aqueous solution forming a protein nanocontainer composed of an inner gel core and an outer protein shell. Two model drugs were loaded into the designed nanocontainers by dissolving drugs in the oil phase before sonication. The loading capacity was up to 0.9 mg/mL for the amphiphilic drug rifampicin, while it reached to 19 mg/mL for the hydrophobic drug indomethacin. The encapsulated drugs were released at different temperatures. At 37 degrees C, only less than 20% of the drug was released due to the protection by the gel core. Increasing temperature to 40 degrees C led to a completely release of the remaining drug. The drug release showed drastic temperature dependence. The biocompatibility of the protein nanocontainers was evaluated by incubating the nanocontainers in the 3T3 cell and B-LCL cell lines. Both experiments indicated an excellent biocompatibility of the designed nanocontainers.