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1.
Front Optoelectron ; 17(1): 9, 2024 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-38584213

RESUMO

This paper presents an efficient scheme for single-pixel imaging (SPI) utilizing a phase-controlled fiber laser array and an untrained deep neural network. The fiber lasers are arranged in a compact hexagonal structure and coherently combined to generate illuminating light fields. Through the utilization of high-speed electro-optic modulators in each individual fiber laser module, the randomly modulated fiber laser array enables rapid speckle projection onto the object of interest. Furthermore, the untrained deep neural network is incorporated into the image reconstructing process to enhance the quality of the reconstructed images. Through simulations and experiments, we validate the feasibility of the proposed method and successfully achieve high-quality SPI utilizing the coherent fiber laser array at a sampling ratio of 1.6%. Given its potential for high emitting power and rapid modulation, the SPI scheme based on the fiber laser array holds promise for broad applications in remote sensing and other applicable fields.

2.
Plant Cell ; 36(5): 1736-1754, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38315889

RESUMO

Roses are among the most popular ornamental plants cultivated worldwide for their great economic, symbolic, and cultural importance. Nevertheless, rapid petal senescence markedly reduces rose (Rosa hybrida) flower quality and value. Petal senescence is a developmental process tightly regulated by various phytohormones. Ethylene accelerates petal senescence, while gibberellic acid (GA) delays this process. However, the molecular mechanisms underlying the crosstalk between these phytohormones in the regulation of petal senescence remain largely unclear. Here, we identified SENESCENCE-ASSOCIATED F-BOX (RhSAF), an ethylene-induced F-box protein gene encoding a recognition subunit of the SCF-type E3 ligase. We demonstrated that RhSAF promotes degradation of the GA receptor GIBBERELLIN INSENSITIVE DWARF1 (RhGID1) to accelerate petal senescence. Silencing RhSAF expression delays petal senescence, while suppressing RhGID1 expression accelerates petal senescence. RhSAF physically interacts with RhGID1s and targets them for ubiquitin/26S proteasome-mediated degradation. Accordingly, ethylene-induced RhGID1C degradation and RhDELLA3 accumulation are compromised in RhSAF-RNAi lines. Our results demonstrate that ethylene antagonizes GA activity through RhGID1 degradation mediated by the E3 ligase RhSAF. These findings enhance our understanding of the phytohormone crosstalk regulating petal senescence and provide insights for improving flower longevity.


Assuntos
Etilenos , Proteínas F-Box , Flores , Regulação da Expressão Gênica de Plantas , Giberelinas , Proteínas de Plantas , Rosa , Etilenos/metabolismo , Etilenos/farmacologia , Giberelinas/metabolismo , Giberelinas/farmacologia , Proteínas F-Box/metabolismo , Proteínas F-Box/genética , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Rosa/genética , Rosa/efeitos dos fármacos , Rosa/metabolismo , Flores/genética , Flores/efeitos dos fármacos , Flores/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Reguladores de Crescimento de Plantas/metabolismo , Reguladores de Crescimento de Plantas/farmacologia , Senescência Vegetal/genética , Complexo de Endopeptidases do Proteassoma/metabolismo , Receptores de Superfície Celular/metabolismo , Receptores de Superfície Celular/genética
3.
Appl Opt ; 62(36): 9446-9453, 2023 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-38108768

RESUMO

A dual aspheric integrated beam shaper suitable for a high-power laser situation has been designed and realized. The model for this lens was derived theoretically and the performance was evaluated using a detailed simulation. The ultrasonic vibration assisted cutting and the high-precision grinding and polishing technology were used for the processing. The surface accuracy was less than 200 nm measured with a profiler, and the roughness was smaller than 20 nm with the help of the white light interferometer. Shaping experiments were carried out, which verified that the Gaussian beam has uniform intensity distribution with a uniformity of 85.13% in the near field and converges to a point in the far field, which is exactly as expected. It thus provides an actual selection for high-power laser shaping.

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