Characteristics of high-risk HPV infection in women with vaginal intraepithelial neoplasia in Beijing, China.

We evaluated the characteristics of high-risk human papillomavirus (Hr-HPV) infection in different grades of vaginal intraepithelial neoplasia (VaIN). 7469 participants were involved in this study, of which 601 were diagnosed with VaIN, including single vaginal intraepithelial neoplasia (s-VaIN, n = 369) and VaIN+CIN (n = 232), 3414 with single cervical intraepithelial neoplasia (s-CIN), 3446 with cervicitis or vaginitis and 8 with vaginal cancer. We got those results. First, the most popular HPV genotypes in VaIN were HPV16, 52, 58, 51, and 56. Second, our study showed that higher parity and older age were risk factors for VaIN3 (p < 0.005). Third, the median Hr-HPV load of VaIN+CIN (725) was higher than that of s-CIN (258) (p = 0.027), and the median Hr-HPV load increased with the grade of VaIN. In addition, the risk of VaIN3 was higher in women with single HPV16 infections (p = 0.01), but those with multiple HPV16 infections faced a higher risk of s-VaIN (p = 0.003) or VaIN+CIN (p = 0.01). Our results suggested that women with higher gravidity and parity, higher Hr-HPV load, multiple HPV16 infections, and perimenopause or menopause status faced a higher risk for VaIN, while those with higher parity, single HPV16 infections, and menopause status are more prone to VaIN3.

MVI-TR: A Transformer-Based Deep Learning Model with Contrast-Enhanced CT for Preoperative Prediction of Microvascular Invasion in Hepatocellular Carcinoma.

In this study, we considered preoperative prediction of microvascular invasion (MVI) status with deep learning (DL) models for patients with early-stage hepatocellular carcinoma (HCC) (tumor size ≤ 5 cm). Two types of DL models based only on venous phase (VP) of contrast-enhanced computed tomography (CECT) were constructed and validated. From our hospital (First Affiliated Hospital of Zhejiang University, Zhejiang, P.R. China), 559 patients, who had histopathological confirmed MVI status, participated in this study. All preoperative CECT were collected, and the patients were randomly divided into training and validation cohorts at a ratio of 4:1. We proposed a novel transformer-based end-to-end DL model, named MVI-TR, which is a supervised learning method. MVI-TR can capture features automatically from radiomics and perform MVI preoperative assessments. In addition, a popular self-supervised learning method, the contrastive learning model, and the widely used residual networks (ResNets family) were constructed for fair comparisons. With an accuracy of 99.1%, a precision of 99.3%, an area under the curve (AUC) of 0.98, a recalling rate of 98.8%, and an F1-score of 99.1% in the training cohort, MVI-TR achieved superior outcomes. Additionally, the validation cohort's MVI status prediction had the best accuracy (97.2%), precision (97.3%), AUC (0.935), recalling rate (93.1%), and F1-score (95.2%). MVI-TR outperformed other models for predicting MVI status, and showed great preoperative predictive value for early-stage HCC patients.

Human papillomavirus integration perspective in small cell cervical carcinoma.

Small cell cervical carcinoma (SCCC) is a rare but aggressive malignancy. Here, we report human papillomavirus features and genomic landscape in SCCC via high-throughput HPV captured sequencing, whole-genome sequencing, whole-transcriptome sequencing, and OncoScan microarrays. HPV18 infections and integrations are commonly detected. Besides MYC family genes (37.9%), we identify SOX (8.4%), NR4A (6.3%), ANKRD (7.4%), and CEA (3.2%) family genes as HPV-integrated hotspots. We construct the genomic local haplotype around HPV-integrated sites, and find tandem duplications and amplified HPV long control regions (LCR). We propose three prominent HPV integration patterns: duplicating oncogenes (MYCN, MYC, and NR4A2), forming fusions (FGFR3-TACC3 and ANKRD12-NDUFV2), and activating genes (MYC) via the cis-regulations of viral LCRs. Moreover, focal CNA amplification peaks harbor canonical cancer genes including the HPV-integrated hotspots within MYC family, SOX2, and others. Our findings may provide potential molecular criteria for the accurate diagnosis and efficacious therapies for this lethal disease.

Optimization Algorithm of Urban Rail Transit Network Route Planning Using Deep Learning Technology.

Under the present background, optimizing the existing urban rail transit network is the focus of urban rail transit construction at present. Based on DL, this paper constructs the optimization algorithm of urban rail transit network route planning. According to the current urban layout and urban planning, build a suitable rail transit network line form; according to the function, the types of urban rail transit stations are divided, and the optimization of urban rail transit network lines is realized. In addition, according to the K short path algorithm, this paper calculates the effective path between any stations of rail transit and, according to the model, allocates the passenger flow to each path. Experimental results show that the accuracy of real-time traffic flow prediction by this algorithm can reach 94.98%, which is about 9% higher than other methods. This algorithm can effectively optimize the route planning of urban rail transit network. This verifies the effectiveness of the route planning optimization algorithm proposed in this paper. Using the algorithm in this paper for line planning can get good real time, rationality, and optimality.

Intelligent Measurement and Analysis of Sewage Treatment Parameters based on Fuzzy Neural Algorithm with ARM9 Core CPU.

After entering the new century, the state continues to increase the construction of urban sewage treatment projects in response to the deteriorating water pollution situation. How to collect and analyze the sewage parameter variables in the sewage treatment process to ensure the intelligent measurement and accurate operation of the parameters in the treatment application is an urgent problem to be solved. This paper is mainly based on the computer-aided control system built by the ARM9 core embedded chip, and the feasibility and effectiveness of fuzzy neural network algorithms are discussed to improve the intelligent processing of sewage treatment parameters. After analyzing the principle and implementation flow of fuzzy control and neural network, starting from the characteristics of data collected by ARM9 core chip, the hybrid algorithm model is optimized and improved to further improve the convergence speed and accuracy of the algorithm. The simulation experiment proves that the optimized fuzzy neural control algorithm can effectively identify the dissolved oxygen, nitrate nitrogen, and other parameter data in the sewage treatment, and the recognition accuracy is very close to the true precision. Based on biosensors, the ARM9 core chip control system established by a recursive fuzzy neural network can greatly improve the tracking and control ability of parameters such as dissolved oxygen concentration and nitrate nitrogen concentration in microbial degradation. This has a good development prospect in wastewater treatment control applications. The experimental results show that the recursive fuzzy neural network algorithm proposed in this paper can dynamically track and control the nitrate concentration and dissolved oxygen concentration and ensure that the control is within the accuracy range. The accuracy of recognition is very close to the real accuracy.

Significance of interstitial fibrosis and p16 in papillary thyroid carcinoma.

We enrolled 264 patients with papillary thyroid carcinoma (PTC). We performed immunohistochemical detection of p16 and determined the degree of interstitial fibrosis (IF). The expression of p16 was associated with pathological tumor-node-metastasis (pTNM) stage and age (p < 0.05). The cancer-specific survival (CSS) was longer in p16-negative patients (195.73 vs. 181.78 months, p = 0.007). p16 was significantly related to the degree of IF (r = 0.130, p = 0.035). PTC patients with no or mild fibrosis tended to have a larger tumor (p = 0.045). The degree of fibrosis was related to the proportion of papillary structure components (p = 0.025). Univariate and multivariate survival analyses showed that relapse-free survival (RFS) was longer in patients with moderate/severe IF (p < 0.05). In summary, p16 was correlated with prognosis and IF of PTC. Patients with moderate/severe IF tend to have better prognosis in RFS.

Preparation and vapor-sensitive properties of hydroxyl-terminated polybutadiene polyurethane conductive polymer nanocomposites based on polyaniline-coated multiwalled carbon nanotubes.

Polyaniline-coated multi-walled carbon nanotube (MWCNT) conductive polymer precursors (MWCNTs@PANI) were prepared by an in situ microemulsion oxidation polymerization of aniline in the case of MWCNTs, and then hydroxyl-terminated polybutadiene polyurethane conductive polymer nanocomposites based on MWCNTs@PANI (MWCNTs@PANI/HTPB PUs) were prepared through an in situ stepwise polymerization of HTPB and diisocyanates. The chemical structure was characterized by Fourier transform infrared spectroscopy (FTIR), Raman, x-ray diffraction, x-ray photoelectron spectroscopy and thermogravimetric analysis. The morphologies and dispersion behavior were examined by scanning electron microscopy, transmission electron microscopy and UV-vis transmittance. The MWCNTs@PANI/HTPB PUs nanocomposites were fabricated into film sensors for detection of volatile organic compound vapors, and displayed an evident response to trichloromethane vapor (CHCl3). The effect of MWCNTs on the conductivity and the responsivity to trichloromethane of conductive polymer nanocomposite films were studied, finding that the conductive composite films have fast and strong response, good repeatability and recoverability, and long-term stability. Consequently, they can be potentially applied for supervision and detection of interior and outdoor environmental gases or vapors.

Improving the Surgical Effect for Primary Liver Cancer with Intraoperative Fluorescence Navigation Compared with Intraoperative Ultrasound.

BACKGROUND This study aimed to compare the application value of intraoperative fluorescence navigation technology (FNT) and intraoperative ultrasound (IOUS) in primary liver cancer surgery. MATERIAL AND METHODS Fifty consecutive patients with primary liver cancer scheduled to receive surgical treatment were divided into FNT group and IOUS group. FNT and IOUS were separately used to guide tumor resection and detect new cancerous lesions in the 2 groups. The complete tumor resection rate (R0) resection rate, length of the tumor distance from cutting edge, the diagnostic efficacy of cancerous nodules and the fluorescence imaging characteristics of different types tumors were recorded. RESULTS The R0 resection rate was 100% (25 out of 25 patients) in the FNT group and 96% (24 out of 25 patients) in the IOUS group. In the FNT group, 1 case (4%, 1 out of 25 patients) had cancer tissue that was less than 1 cm from the cutting edge, compared to 7 cases (28%, 7 out of 25 patients) in the IOUS group (P=0.049), which was a significant difference. In the remaining livers of 50 consecutive patients, FNT found 5 new cancerous nodules with a sensitivity of 71.4%, a specificity of 11.1%, and a false-positive rate of 88.9%; for IOUS the results were 42.9%, 88.9%, 11.1%. The fluorescence imaging characteristics of all well-differentiated hepatocellular carcinomas were tumor tissue imaging, but all other types of tumors were ring imaging around the tumor. CONCLUSIONS FNT can improve the R0 resection rate, ensure a safe distance between tumor and cutting edge and can identify more new cancerous nodules compared to IOUS. Thus, FNT could improve the surgical treatment effect for primary liver cancer and hopefully further improve the prognosis of patients.

Role of ultrasonographic features and quantified BRAFV600E mutation in lymph node metastasis in Chinese patients with papillary thyroid carcinoma.

The association between cervical lymph node metastasis (LNM) and ultrasonographic features as well as BRAFV600E mutations in patients with papillary thyroid carcinoma (PTC) remained controversial. This study investigated the association between LNM and ultrasonographic features as well as BRAFV600E mutation in Chinese patients with PTC. A total of 280 patients with PTC in China were included in this study. 108 had cervical lymph node metastasis, while 172 had not. Younger age (<45years) and several ultrasonographic features were significantly associated with cervical LNM (Ps < 0.05). The BRAFV600E mutation was detected in 81.0% of patients with PTC (226/280). The status of BRAFV600E mutation was not associated with cervical LNM. However, Ct values by PCR and intensity of reactions by immunohistochemistry (IHC) for BRAFV600E expression had shown significant difference between group with and without LNM. Furthermore, an increased proportion of LNM was also found with the incremental intensity of IHC for BRAFV600E expression from weak to strong reaction after adjusted potential confounders. Further studies are required to verify this association and explore the intrinsic mechanism.

Clinicopathological analysis of non-gestational ovarian choriocarcinoma: Report of two cases and review of the literature.

The aim of the present study was to analyze the clinicopathological features of two cases of non-gestational ovarian choriocarcinoma (NGCO). The histopathological, immunohistochemical and clinical features of two cases of NGCO in the left ovaries of two 13 year-old female patients were investigated and the relevant literature was reviewed. In both cases, the tumor masses exhibited cribriform, papillary and nested cellular growth patterns, and hemorrhage and necrosis were evident. In case one, the patient also exhibited a sex cord tumor with annular tubules (SCTAT) in the right ovary. To the best of our knowledge, the synchronous occurrence of these two tumor types has not been reported previously. Immunohistochemically, the tumor cells of choriocarcinoma in both cases were positive for human chorionic gonadotropin and cytokeratin, while those of SCTAT were positive for CD56 and CD99. NGCO is an extremely rare germ cell tumor of high-grade malignancy, and STCAT is even rarer. Early metastasis of NGCO is common and the disease has a poor prognosis. In the present study, one patient succumbed within 4 months of diagnosis with NGCO and the other patient was lost to follow-up after 12 months.

[Clinicopathologic study of 25 cases of placental chorioangioma].

OBJECTIVE: To investigate the clinicopathological characteristics, diagnostic criteria and differential diagnosis of placental chorioangioma. METHODS: Twenty-five cases of placental chorioangioma were analyzed for their clinical data, histomorphology and immumohisto chemical staining. Relevant literature was reviewed. RESULTS: The average age of the 25 patients was 29 years. Fourteen patients had full-term pregnancy, 10 had preterm labor, and 1 had intrauterine fetal death. Nineteen patients had pregnancy complications. The tumors presented as red or dusty pink nodules with clear borders. The tumor size ranged from 1 to 16 cm. Microscopically, the tumors possessed abundant capillaries or cavernous blood spaces lined by hyperplastic endothelial cells. These cells were positive for CD34 and Ki-67 index < 10%. CONCLUSIONS: Placental chorioangioma is a rare benign tumor of the placenta, and is associated with various pregnancy complications. Misdiagnosis of cell-rich type tumor should be avoided.

Simulation and experimental study of aspect ratio limitation in Fresnel zone plates for hard-x-ray optics.

For acquiring high-contrast and high-brightness images in hard-x-ray optics, Fresnel zone plates with high aspect ratios (zone height/zone width) have been constantly pursued. However, knowledge of aspect ratio limits remains limited. This work explores the achievable aspect ratio limit in polymethyl methacrylate (PMMA) by electron-beam lithography (EBL) under 100 keV, and investigates the lithographic factors for this limitation. Both Monte Carlo simulation and EBL on thick PMMA are applied to investigate the profile evolution with exposure doses over 100 nm wide dense zones. A high-resolution scanning electron microscope at low acceleration mode for charging free is applied to characterize the resultant zone profiles. It was discovered for what we believe is the first time that the primary electron-beam spreading in PMMA and the proximity effect due to extra exposure from neighboring areas could be the major causes of limiting the aspect ratio. Using the optimized lithography condition, a 100 nm zone plate with aspect ratio of 15/1 was fabricated and its focusing property was characterized at the Shanghai Synchrotron Radiation Facility. The aspect ratio limit found in this work should be extremely useful for guiding further technical development in nanofabrication of high-quality Fresnel zone plates.

Gold nanopillar arrays as biosensors fabricated by electron beam lithography combined with electroplating.

We report our work on the development of subwavelength gold pillar arrays as local surface plasmonic (LSP) resonators for sensor applications. These arrays are fabricated by electron beam lithography combined with electroplating. The conical shape, instead of flat one, on the top of Au pillars, induced by uneven current density in the plating, may affect the LSP resonance (LSPR). This paper aims to carry out a systematic study of LSPR behavior in nanopillar arrays with both flat and conical shapes on the top, trying to prove the feasibility of the developed nanoprocess. Both numerical simulations by the finite-difference time-domain (FDTD) method and experimental characterization on fabricated LSP resonators for reflectance spectra were carried out. Our experiments indicate that the fabricated nanopillar arrays in Au demonstrate the promising capability of refractive index sensing with sensitivity of 270 nm/refractive index unit. FDTD simulation of electric field density in the gap between pillars reveals the correlation between the resonant absorption of the incident light and the standing waves of localized surface plasmon polaritons in the gaps of the pillar array, despite the conical shape of the pillars. Moreover, it was discovered that the resonant absorption becomes stronger when the light incident angle is increased. The proposed nanoprocess for pillar arrays should possess great prospects for manufacturing Au pillars with high aspect ratio for achieving higher sensitivity at an economical cost.

Clinicopathological analysis on small cell carcinoma of the prostate in chinese patients.

OBJECTIVE: To explore the clinical and pathological characteristics of small-cell carcinoma (SmCC) of the prostate and applicable treatment methods. METHODS: We reported three cases of SmCC of the prostate diagnosed from 1999 to 2011 at the Chinese PLA General Hospital. We also reviewed clinical and pathological data of 26 cases in China reported over the same period. RESULTS: Serum prostate-specific antigen (PSA) levels were normal in 20 cases (76.9%) and elevated in six (23.1%). There was local invasion in 12 cases (46.2%) at the time of diagnosis; lymphatic vessel invasion and distant metastases were detected in eight (30.8%) and nine cases (34.6%) respectively. At the end of follow-up, 16 cases (61.5%) had died, eight (30.8%) survived, and two (7.7%) were missing. The median survival time was 8 months, and the 1-year survival rate was 23.2%. Statistical analysis showed that survival time was significantly correlated with chemotherapy treatment (p<0.05). However, serum PSA levels, surgical approach, pathological type, local invasion, lymphatic vessel invasion, and distant metastasis had no significant relationship with survival (p>0.05). CONCLUSIONS: SmCC of the prostate is a rare neoplasm typified by high malignancy, rapid progress, and poor prognosis. Pathological analysis is an important tool for confirming a diagnosis. Pure SmCC is usually not associated with an increase in serum PSA. Surgery, mixed with acinar adenocarcinoma components, and clinical staging do not correlate with prognosis; and chemotherapy was the only prognostic factor. For patients, diagnosed by preoperative biopsy, administering chemotherapy as the first-line treatment may improve outcomes.

[Clinicopathologic analysis of 14 cases of atypical polypoid adenomyoma of endometrium with the emphasis on its cancerous transformation].

OBJECTIVE: To analyze the clinicopathologic characteristics of atypical polypoid adenomyoma (APA) of endometrium, and investigate the special characteristics of cancerous transformation from APA. METHODS: Fourteen cases of APA were collected in General Hospital of People's Liberation Army from January 2007 to March 2013. The clinical data, morphologic features, immunohistochemistry and the related literature were reviewed. RESULTS: The median age of the 14 patients was 38 years (ranged from 23 to 72 years), only 1 patient was postmenopausal. The most common symptom was irregular vaginal bleeding (4/14), and 4 patients were identified during routine physical examination for infertility. Among 14 cases, 4 cases were diagnosed as well differentiated endometrioid adenocarcinoma originating from APA, and their median age was 35 years (ranged from 28 to 41 years); color Doppler flow imaging (CDFI) of ultrasound showed rich blood flow signal. The tumors with cancerous components were obviously larger than the usual APA (mean diameter: 4.7 versus 1.8 cm). Histologically, irregular and branched glands were embedded in fibromuscular stroma and the glandular epithelium were atypical hyperplasia in varying degrees. While carcinoma developed in the APA, the sieve, solid and papillary structures were noticeable, and necrosis were common. CONCLUSIONS: APA is a rare lesion of the uterus. Although the clinical behavior is benign in most cases, there may be possible for some cases developing carcinomas. If the APA mass is more than 4 cm in diameter, and microscopically demonstrates prominent sieve, solid, papillary structures and necrosis, the diagnosis of carcinoma developed from APA can be made. Thorough analysis should be done before the most proper therapeutic regimen is drawn up.