Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros








Base de dados
Assunto principal
Intervalo de ano de publicação
1.
PLoS One ; 19(5): e0303273, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38781236

RESUMO

Lithified layers of complex microbial mats known as microbialites are ubiquitous in the fossil record, and modern forms are increasingly identified globally. A key challenge to developing an understanding of microbialite formation and environmental role is how to investigate complex and diverse communities in situ. We selected living, layered microbialites (stromatolites) in a peritidal environment near Schoenmakerskop, Eastern Cape, South Africa to conduct a spatial survey mapping the composition and small molecule production of the microbial communities from environmental samples. Substrate core samples were collected from nine sampling stations ranging from the upper point of the freshwater inflow to the lower marine interface where tidal overtopping takes place. Substrate cores provided material for parallel analyses of microbial community diversity by 16S rRNA gene amplicon sequencing and metabolomics using LC-MS2. Species and metabolite diversities were correlated, and prominent specialized metabolites were targeted for preliminary characterization. A new series of cyclic hexadepsipeptides, named ibhayipeptolides, was most abundant in substrate cores of submerged microbialites. These results demonstrate the detection and identification of metabolites from mass-limited environmental samples and contribute knowledge about microbialite chemistry and biology, which facilitates future targeted studies of specialized metabolite function and biosynthesis.


Assuntos
Metabolômica , Metabolômica/métodos , África do Sul , RNA Ribossômico 16S/genética , Sedimentos Geológicos/microbiologia , Depsipeptídeos/biossíntese , Depsipeptídeos/química , Bactérias/metabolismo , Bactérias/genética , Bactérias/classificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA