Antioxidant and pro-oxidant actions of resveratrol on human serum albumin in the presence of toxic diabetes metabolites: Glyoxal and methyl-glyoxal.

Methylglyoxal (MGO) and glyoxal (GO) are attracting considerable attention because of their role in the onset of diabetes symptoms. Therefore, to comprehend the molecular fundamentals of their pathological actions is of the utmost importance. In this study, the molecular interactions between resveratrol (RES) and human serum albumin (HSA) and the ability of the stilbene to counteract the oxidative damage caused by pathological concentrations of MGO and GO to the human plasma protein, was assessed. The oxidation of Cys34 in HSA as well as the formation of specific protein semialdehydes AAS (α-aminoadipic), GGS (γ-glutamic) and the accumulation of Advanced Glycation End-products (AGEs) was investigated. Resveratrol was found to neutralize both α-dicarbonyls by forming adducts detected by HESI-Orbitrap-MS. This antioxidant action was manifested in a significant reduction of AGEs. However, RES-α-dicarbonyl conjugates oxidized Cys34 and lysine, arginine and/or proline by a nucleophilic attack on SH and ε-NH groups in HSA. The formation of specific semialdehydes in HSA after incubation with GO and MGO at pathological concentrations was reported for the first time in this study, and may be used as early and specific biomarkers of the oxidative stress undergone by diabetic patients. The pro-oxidative role of the RES-α-dicarbonyl conjugates should be further investigated to clarify whether this action leads to positive or harmful clinical consequences. The biological relevance of human protein carbonylation as a redox signaling mechanism and/or as a reflection of oxidative damage and disease should also be studied in future works.

Shelf life of cooked goat blood sausage prepared with the addition of heart and kidney.

This study focused on the effect of two packaging formats (vacuum packaging and over-wrap packaging) on the shelf life of cooked sausage prepared with blood, heart, kidney and goat meat fragments under storage at 4±1°C for a period of 90 days. The storage time and type of packaging significantly affected the chemical (pH, moisture, protein and TBARS number), physical (shear force) and microbial (mould and yeast) parameters of cooked sausage. Vacuum packaging maintained the microbiological and chemical qualities of cooked goat blood sausage for a longer period of time (63 days) compared to over-wrap packaging (41 days) and could be a viable alternative to refrigerated storage of the product for quality maintenance.

Nutritional and sensory characteristics of Minas fresh cheese made with goat milk, cow milk, or a mixture of both.

This study aimed to assess and compare the nutritional, technological, and sensory characteristics of Minas fresh cheese made with goat milk, cow milk, or a mixture of the two stored in cold conditions for 21d. The yield and centesimal composition of the cheeses were not affected by the type of milk used in their preparation. Reductions were observed in the moisture content, pH, proteolysis index, and instrumental hardness; moreover, increases were observed in the syneresis, acidity index, and depth of proteolysis index in all cheeses. The percentages of caprylic, capric, oleic, and linoleic fatty acids were higher in goat milk cheese and cheese made with a mixture of goat and cow milk compared with cow milk cheese, and a sensory evaluation revealed differences in color, flavor, and aroma between the cheeses. The preparation of Minas fresh cheese with a mixture of goat and cow milk can be a viable alternative for dairy products in the market that can be characterized as high-quality products that meet consumer demands.

Genotype effect on carcass and meat quality of lambs finished in irrigated pastures in the semiarid Northeastern Brazil / Efeito do genótipo sobre características de carcaça e qualidade da carne de cordeiros terminados em pastagem irrigada no semiárido do Nordeste do Brasil

The aim of this study was to compare four genetic groups of sheep on the carcass and meat quality traits. Thirty-three contemporary and unrelated male lambs, all of single birth were used in the experiment, being thirteen from the Santa Inês (SI) breed, seven from the Brazilian Somali breed (BS), six from the Morada Nova (MN) breed and seven from the ½ Dorper - ½ Morada Nova (F1) crossbreed. The genotypes SI, BS and F1 presented similar performances in relation to hot and cold carcass weights, which values were 10.76±0.53kg and 10.46±0.52kg for SI, 9.20±0.73kg and 8.99±0.71kg for BS, and 9.35±0.73kg and 9.13±0.71kg for F1, respectively. The BS had a better hot carcass yield (47.10±0.88%) and cold carcass yield (46.00±0.87%). Better carcass conformation was observed in SI and F1 (2.73±0.12 and 2.50±0.17, respectively) while the BS presented a better finishing (3.29±0.20). The average for the rib eye area (REA) was 9.94±0.49cm², 8.66±0.67cm², 7.18±0.72cm² and 9.8±0.67cm², and for the carcass compactness index (CCI) it was 0.17±0.01kg/cm, 0.17±0.01kg/cm, 0.11±0.01kg/cm and 0.16±0,01kg/cm, for SI, SB, MN and F1, respectively. There were no significant differences between SI, BS and F1 regarding REA and CCI. Although, in general, the MN presented a relatively lower performance than the other genotypes, this breed had similar carcass yields and fat thickness when compared to SI and F1 and similar conformation and REA in comparison to the BS. Regarding meat quality, no differences were observed between genotypes, except for redness and cooking losses. It is concluded that no one group had a higher or lower performance in all traits analyzed. Moreover, for the management conditions employed in this study, there was evidence of greater specialization in meat production for genotypes SI, BS and F1 when compared to MN, although there are no substantial differences between the four groups regarding meat quality.

O objetivo deste estudo foi comparar quatro grupos genéticos de ovinos quanto às características de carcaça e de qualidade da carne. Trinta e três cordeiros, contemporâneos, nascidos de parto simples e sem relação de parentesco, foram utilizados no experimento, sendo 13 da raça Santa Inês (SI), sete da raça Somalis Brasileira (SB), seis da raça Morada Nova (MN) e sete ½ Dorper - ½ Morada Nova (F1). Os genótipos SI, SB e F1 apresentaram desempenhos similares quanto ao peso das carcaças quente e fria, cujos valores foram 10,76±0,53kg e 10,46±0,52kg para o SI, 9,20±0,73kg e 8,99±0,71kg para o SB, 9,35±0,73kg e 9,13±0,71kg para o F1, respectivamente. A raça SB apresentou melhor rendimento de carcaça quente (47,10 ±0,88%) e de carcaça fria (46,00±0,87%). Os genótipos SI e F1 apresentaram melhores conformações (2,73±0,12 e 2,50±0,17, respectivamente), enquanto o SB apresentou melhor acabamento (3,29±0,20). As médias para área de olho de lombo (AOL) foram 9,94±0,49cm², 8,66±0,67cm², 7,18±0,72cm² e 9,8±0,67cm² e para o índice de compacidade da carcaça (ICC) foram 0,17±0,01kg/cm, 0,17±0,01kg/cm, 0,11±0,01kg/cm e 0,16±0,01kg/cm, para SI, SB, MN e F1, respectivamente. Não houve diferença significativa entre os genótipos SI, SB e F1 quanto à AOL e ao ICC. Apesar de, no geral, a raça MN apresentar desempenho relativamente inferior aos demais genótipos quanto às características de carcaça analisadas, essa raça apresentou rendimentos de carcaça e espessura de gordura similar ao SI e F1 e conformação e AOL similar ao SB. Não houve um grupo superior ou inferior para todas as características analisadas. Por outro lado, para as condições de manejo empregadas neste estudo, evidenciou-se maior especialização para produção de carne dos genótipos SI, SB e F1 em relação ao grupo MN. Não houve diferenças importantes entre os diferentes genótipos quanto à qualidade da carne.

The chemical and sensory qualities of smoked blood sausage made with the edible by-products of goat slaughter.

The aim was to evaluate smoked blood sausage prepared using goat blood (50%), viscera (10%) and meat fragments (20%). Microbiological, chemical and sensory evaluations were conducted. The quality analyses showed that smoked goat blood sausage is rich in high biological value proteins, amino acids, essential fatty acids, and iron (26.65mg/100g). The smoked goat blood sausage was rated to have a sensory acceptance of greater than 80%. The use of edible by-products from the slaughter of goats in the formulation of smoked blood sausage is viable because it uses low-cost raw materials; furthermore, the utilisation of these by-products can generate income for producers, allowing them to offer a meat product of high nutritional and sensory quality.

Effect of silk flower hay (Calotropis procera Sw) feeding on the physical and chemical quality of Longissimus dorsi muscle of Santa Inez lambs.

The effect of different levels of silk flower hay (Calotropis procera Sw) in the diet of confined lambs by the substitution of the commonly used foraging broom corn hay (Sorghum bicolor L) was investigated to evaluate its possible effects on the growth and quality of the lamb meat. Twenty-four male Santa Inez lambs were divided in equal numbers into four treatment groups and fed diets containing 0%, 16.7%, 33.3% and 50% of silk flower hay (SFH). Growth rate, feed intake and meat quality were investigated. Mean daily gains of lambs were 170g for control, 180.5g for 16.7% SFH, 96.8g for 33.3% SFH and 22.9g for 50% SFH. The use of silk flower hay in the diet of Santa Inez lambs affected health of the animals and meat pH when the substitution of the forage was high, up to 50%; however, the general meat quality was not affected. Meat from animals fed with levels up to 50% SFH had physical (Aw, a(∗), b(∗) and L(∗)) and chemical (moisture, ash, lipid, phosphorus, iron, phospholipid and fatty-acid profile) parameters comparable to the control group (0% SFH). Among the various levels of substitution (16.7%, 33.3% and 50%) of silk flower hay in the diet tried in this work, the use of 16.7% was found to be an attractive and technically viable option for the Northeast region of Brazil.

Meat quality of Moxotó and Canindé goats as affected by two levels of feeding.

The objective of this study was to evaluate the effect of reduced feed intake on Moxotó and Canindé goat, which are native breeds of semi arid area of Brazil, by analyzing the resultant meat quality. Intact male goats of both genotypes (10 animals each), weighing about 15kg, were allocated to two feeding levels (AL: feed ad libitum and FR: feed restricted to 63% of the total feed consumed by AL). Water-holding capacity and a(∗) color parameter were significantly influenced (p<0.05) by genotype. Proximate composition, cholesterol, shear force, pH, cooking loss, and sensory scores did not differ significantly (p>0.05) between genotypes. The redness of the meat of the Canindé breed was much improved in FR goats. Although, feed restriction by 37% did affect the final live weight and mean daily gain weight, it had no effect on chemical, physical, and sensory quality of Moxotó and Canindé meat.

Características microbiológicas da carne de caprinos submetidos a diferentes formas de manejo / Characteristics microbiological of the meat of goats submitted to peculiar forms of management

A manipulação da carcaça no abate propicia a proliferação de microorganismos. A produção de carne caprina que atenda aos requisitos de segurança microbiológica agrega valor ao produto, além de traduzir a eficiência do processamento em todas as suas etapas. O objetivo deste trabalho foi observar se o perfil microbiológico da carne caprina dos animais pesquisados encontra-se de acordo com padrões propostos na Resolução - RDC n. 12, de 2 de janeiro de 2001, que define o Regulamento Técnico sobre Padrões Microbiológicos para Alimentos. Determinou-se o pH, a atividade de água e o número mais provável de coliformes totais e fecais, Salmonella sp e Staphylococcus coagulase positiva. Amostras constituídas pelo conjunto de músculos que formam a perna do caprino (total de 24 animais) foram analisadas. Os resultados demonstraram que, de um modo geral, amostras desse estudo atenderam aos requisitos de segurança microbiológica da referida RDC, com exceção pra um dos grupos pesquisados (C - 30), que apresentou uma concentração de Coliformes fecais de 2,1 x 10 , valor este acima do estabelecido pela legislação vigente que é de 10. Este artigo serve de interesse para cientistas de alimentos que pesquisam a carne, e microbiologistas que trabalham com saúde pública e se interessam pela segurança das carnes em geral e da carne caprina em particular.

Castration and slaughter age effects on panel assessment and aroma compounds of the "mestiço" goat meat.

Cooked meat from castrated male and intact "mestiço" goats slaughtered at 175, 220, 265 and 310 days of age, were analysed by panellists and GC-MS. Slaughter age had a (P<0.05) effect on organoleptic qualities of goat meat. Scores for sensorial attributes were not different (P<0.05) for intact and castrated goats. A total of 87 compounds were identified and a further 21 were partially characterized by GC-MS. Hydrocarbons were presented in the highest relative amounts and all aroma isolates were dominated by products of lipid degradation. Total relative abundance of volatiles was identified at higher levels in the cooked meat from castrated goats than from entires. Meat from goats slaughtered at first age (175 days) was preferred by panellists and was found to have the lowest number of volatiles and total relative abundance.

Castration and slaughter age effects on nutritive value of the "mestiço" goat meat.

Groups of castrated male and intact "mestiço" goats were slaughtered at 175, 220, 265 and 310 days of age. After slaughter the carcasses were chilled for 24 h at 2°C, and pH, Aw, Ca, Fe, P, moisture, ash, protein and fat were determined on the total lean. Slaughter age had a significant (p<0.01) effect on moisture, protein, iron, calcium and pH. Calcium content was influenced by castration and the slaughter age×castration interaction was significant (p<0.01) for iron content. Meat from castrated males contained less moisture and protein and more fat, ash, iron and calcium than did meat from intact males. The fat, protein and iron content increased with increasing slaughter age, while moisture decreased. Composite results indicate slaughtering "mestiço" goats at 310 days exert favourable effects on the parameters investigated. ©