Caracterização da agressão verbal com enfermeiros em uma unidade hospitalar / Characterization of verbal aggression with nurses in a hospital unit / Caracterización de la agresión verbal con enfermeras en una unidad hospitalaria

Objetivo: O objetivo do estudo é caracterizar a agressão verbal com enfermeiros de uma unidade hospitalar. Métodos: Trata-se de um estudo descritivo, quantitativo e de corte transversal, realizado em uma unidade hospitalar da região centro-sul cearense, no ano de 2019. A amostra foi composta por 43 enfermeiros, submetidos a uma pesquisa semiestruturada, os dados foram tabulados em um sistema computacional para a elaboração de estatísticas, cálculos e tabelas. A violência foi evidenciada como uma preocupação relevante entre os participantes, sendo a agressão verbal a forma mais relevante de violência sofrida. Resultados: Identificou-se a predominância de profissionais adultos jovens, população feminina, sem cargo administrativo e com experiência profissional entre seis e dez anos. A caracterização sociodemográfica dos participantes, corroboraram com achados potenciais de vulnerabilidade. Conclusão: As formas de enfrentamento pelos profissionais, tende a evoluir, muitas vezes, de modo agressivo e negativo, interferindo diretamente nas condições de trabalho. Desse modo, faz-se necessário a elaboração de estratégias para estímulo ao relato de violência, assim como o seu enfrentamento, bem como estudos sobre a temática. (AU)

Objective: The aim of the study is to characterize the verbal aggression whit nurses in a hospital unit. Methods: This is a descriptive, quantitative and cross-sectional study, carried out in a hospital in the central-south region of Ceará, in 2019. The sample consisted of 43 nurses, who underwent a semi-structured survey, the data were tabulated in a computational system for the elaboration of statistics, calculations and tables. Violence was highlighted as a relevant concern among the participants, with verbal aggression being the most relevant form of violence suffered. Results: It was identified the predominance of young adult professionals, female population, without administrative position and with professional experience between six and ten years. The sociodemographic characterization of the participants corroborated with potential vulnerability findings. Conclusion: The ways of coping by professionals tend to evolve, often in an aggressive and negative way, directly interfering with working conditions. Thus, it is necessary to develop strategies to encourage reporting of violence, as well as its confrontation, as well as studies on the subject. (AU)

Objetivo: El objetivo del estudio es caracterizar la agresión verbal con enfermeras en un hospital. Se trata de un estudio descriptivo, cuantitativo y transversal, realizado en una unidad hospitalaria de la región centro-sur de Ceará, en 2019. Métodos: La muestra estuvo compuesta por 43 enfermeros, sometidos a una investigación semiestructurada, los datos fueron tabulados en un sistema computacional para la elaboración de estadísticas, cálculos y tablas. La violencia se evidenció como una preocupación relevante entre los participantes, siendo la agresión verbal la forma más relevante de violencia sufrida. Resultados: Se identificó el predominio de profesionales jóvenes adultos, población femenina, sin cargo administrativo y con experiencia profesional entre seis y diez años. La caracterización sociodemográfica de los participantes corroboró posibles hallazgos de vulnerabilidad. Conclusión: Las formas de afrontamiento de los profesionales tienden a evolucionar, a menudo de forma agresiva y negativa, interfiriendo directamente en las condiciones laborales. Por ello, es necesario desarrollar estrategias para incentivar la denuncia de la violencia, así como su enfrentamiento, así como estudios sobre el tema. (AU)

Violência obstétrica à luz da Teoria da Diversidade e Universalidade do Cuidado Cultural / Obstetric violence in the light of the theory of culture care diversity and universality

Introdução: A violência obstétrica pode ser do tipo física, verbal, psicológica, sexual e negligência da assistência. Não utilização de medicação analgésica, tratamento grosseiro, privação do direito de acompanhante durante o parto, procedimento sem o consentimento da parturiente são exemplos de violência, que está cada vez mais presente e de forma velada nos serviços de saúde brasileiro. Objetivou-se analisar relatos de puérperas sobre violência obstétrica à luz da Teoria da Diversidade e Universalidade do Cuidado Cultural. Materiais e Método: Estudo transversal, abordagem qualitativa, desenvolvido em estratégias de Saúde da Família com 10 puérperas. A coleta de dados foi realizada através de entrevista semi-estruturada cujos resultados foram organizados e adaptados ao modelo Sunrise. Resultados: A maioria das participantes eram jovens, casadas/união estável, primíparas e com parto vaginal. Na adaptação do modelo, considerando seus conceitos, observou-se ausência de conhecimento do parto/trabalho de parto; medo; violência perpetrada contra as mulheres resultantes da ausência de comunicação, desumanização, exposição do corpo e desconforto, repercutindo em cuidado fragilizado, com insatisfação frente ao serviço de saúde. Discussão: A violência obstétrica é comum no cenário brasileiro, acontecendo muitas vezes de forma velada, pois as parturientes não conhecem sobre o assunto, bem como, seus direitos. Conclusão: A violência obstétrica aconteceu por meio do caráter sexual, físico, psicológico e institucional, tornando o ato de parir algo temoroso, resultante do medo, falhas na comunicação e cuidado fragilizado.

Introduction: Obstetric violence can involve physical, verbal, psychological and sexual abuse, and neglect of care. Refusal to administer pain relief, disrespectful treatment, deprivation of women's right to be accompanied during childbirth, medical interventions without their prior consent are some examples of this violence, which is an increasingly present but hidden practice in healthcare services in Brazil. This study aims to analyze the narratives of labouring women on obstetric violence in the light of the theory of culture care diversity and universality. Materials and Methods: A qualitative cross-sectional study developed in the light of the Family Health Strategy was conducted with ten labouring women. Data was collected through a semi-structured interview with results organized and adapted to the Sunrise Model. Results: Most participants were young, married/in a stable relationship, primiparous with vaginal delivery. Based on the model principles, the lack of knowledge about childbirth/labor, medication, violence against women resulting from miscommunication, body exposure and discomfort leads to weakened care provision and dissatisfaction with health services. Discussion: Obstetric violence is a common practice in the Brazilian scenario that often occurs covertly due to the lack of knowledge about its existence and childbirth rights as labouring women. Conclusions: Obstetric violence involves sexual, physical, psychological and institutional abuse, making the act of giving birth seem frightening due to fear, lack of communication and weakened care provision.

Introducción: La violencia obstétrica puede ser de tipo física, verbal, psicológica, sexual y de negligencia asistencial. La negativa a administrar medicamentos analgésicos, el trato brusco, la privación del derecho a estar acompañada durante el parto, el procedimiento sin el consentimiento de la parturienta son una muestra de esta violencia, cada vez más presente, pero de forma disimulada en los servicios asistenciales en Brasil. El objetivo es analizar los relatos de las parturientas sobre la violencia obstétrica a la luz de la teoría de la diversidad y universalidad de los cuidados culturales. Materiales y métodos: Estudio transversal con enfoque cualitativo desarrollado a la luz de la política de Estrategia de Salud de la Familia con diez parturientas. Los datos se recolectaron a través de una entrevista semiestructurada y los resultados se organizaron y adaptaron al modelo de sol naciente. Resultados: La mayoría de las participantes eran jóvenes, casadas/en unión estable, primíparas y con parto vaginal. En la adaptación del modelo, a partir de sus conceptos, se observó la falta de conocimiento del parto/trabajo de parto, la medicación, la violencia perpetrada contra las mujeres resultante de la falta de comunicación, deshumanización, exposición del cuerpo y descontento, lo que repercute en un cuidado debilitado con insatisfacción frente al servicio de salud. Discusión: La violencia obstétrica es común en el escenario brasileño que a menudo ocurre de forma disimulada, debido al desconocimiento de su existencia y de sus derechos como parturientas. Conclusión: La violencia obstétrica pasa por el carácter sexual, físico, psicológico e institucional, lo que hace que el acto de dar a luz se vea como algo aterrador, producto del miedo, la falta de comunicación y la atención debilitada.

Predictors of shorter- and longer-term mortality after COVID-19 presentation among dialysis patients: parallel use of machine learning models in Latin and North American countries.

BACKGROUND: We developed machine learning models to understand the predictors of shorter-, intermediate-, and longer-term mortality among hemodialysis (HD) patients affected by COVID-19 in four countries in the Americas. METHODS: We used data from adult HD patients treated at regional institutions of a global provider in Latin America (LatAm) and North America who contracted COVID-19 in 2020 before SARS-CoV-2 vaccines were available. Using 93 commonly captured variables, we developed machine learning models that predicted the likelihood of death overall, as well as during 0-14, 15-30, > 30 days after COVID-19 presentation and identified the importance of predictors. XGBoost models were built in parallel using the same programming with a 60%:20%:20% random split for training, validation, & testing data for the datasets from LatAm (Argentina, Columbia, Ecuador) and North America (United States) countries. RESULTS: Among HD patients with COVID-19, 28.8% (1,001/3,473) died in LatAm and 20.5% (4,426/21,624) died in North America. Mortality occurred earlier in LatAm versus North America; 15.0% and 7.3% of patients died within 0-14 days, 7.9% and 4.6% of patients died within 15-30 days, and 5.9% and 8.6% of patients died > 30 days after COVID-19 presentation, respectively. Area under curve ranged from 0.73 to 0.83 across prediction models in both regions. Top predictors of death after COVID-19 consistently included older age, longer vintage, markers of poor nutrition and more inflammation in both regions at all timepoints. Unique patient attributes (higher BMI, male sex) were top predictors of mortality during 0-14 and 15-30 days after COVID-19, yet not mortality > 30 days after presentation. CONCLUSIONS: Findings showed distinct profiles of mortality in COVID-19 in LatAm and North America throughout 2020. Mortality rate was higher within 0-14 and 15-30 days after COVID-19 in LatAm, while mortality rate was higher in North America > 30 days after presentation. Nonetheless, a remarkable proportion of HD patients died > 30 days after COVID-19 presentation in both regions. We were able to develop a series of suitable prognostic prediction models and establish the top predictors of death in COVID-19 during shorter-, intermediate-, and longer-term follow up periods.

"EMPODEREENF": construction of an application for nurses' continuing education on psychological violence against women.

OBJECTIVE: to construct a prototype of a mobile application on psychological violence against women to facilitate nurses' continuing education. METHOD: an applied methodological research of technological development, based on the Contextualized Instructional Design method. RESULTS: the prototype content was based on the learning objectives developed through a narrative review, which guided an integrative review to compile the information. The prototype is called "EmpodereEnf", and is composed of an initial screen, bringing nurses as a target audience, and, soon after, offering nine general moblets for access to information such as: concepts, causes, manifestations and consequences of psychological violence; means for identification and approach in nursing consultation and health education; compulsory notification and referral; examples of cases of psychological violence and references. FINAL CONSIDERATIONS: the prototype is a possibility for future coping interventions and a work tool for nurses in the face of psychological violence against women.

Nurse's competences in promoting women's health in light of the Galway Consensus.

OBJECTIVES: to identify nurses' competences in promoting women's health. METHODS: descriptive study with a qualitative approach that adopted the Galway Consensus as a theoretical-methodological framework. Data collection was performed through semi-structured interviews. RESULTS: most of the Galway Consensus' competence domains were present in the nurses' interventions related to health promotion in women's care. "Assessment of needs" and "Implementation" were the most highlighted domains, followed by "Leadership" and "Impact assessment". The domain "Defending/Advocating Rights" was not identified. FINAL CONSIDERATIONS: within the nurses' work with women, some of the Galway Consensus domains of competencies for health promotion were present. However, there is a need, in the context of continuous health education, to expand strategies and enhance the development and application of these health promotion competences.

How is Violence Themed in Nursing Education? Curricular Components in Northeastern Brazil.

It aims to identify how the phenomenon of violence is themed in the curricular components of undergraduate nursing courses of public and private higher education institutions in Northeastern of Brazil.This is a descriptive and exploratory study, with documentary design and qualitative approach, based on the identification of the terms violence/aggression/abuse/aggressive/abusive in discipline menus available online from higher education institutions in northeastern Brazil. The data were organized and processed by the IraMuTeQ software and analyzed in the light of the relevant literature.In total, 412 nursing graduate institutions were found, in which 49 had documents available online, and, of these, 21 had the term violence. The processing of the syllabuses in the program resulted in significant use (79.37%) originating from the Descending Hierarchical Classification, seven classes that reveal the highlight of the approach of violence directed at the female public and in this context, the approach to violence against women was associated with sexual and reproductive aspects, and in a secondary way, it integrated the perspective of female vulnerabilities, power relations, consequences of violence and nursing care to cope with the phenomenon. Violence against children and adolescents and the discussion of violence in the contexts of collective health and health promotion were also approached in a less intense way.There was a predominance of the approach to violence aimed at women's health, under a biological approach, with a deficit in the transversality of the contents. However, it is important to highlight potentialities associated with the approach and teaching of contents that stimulate the critical thinking of the student before female vulnerabilities and power relations between the sexes, which can contribute to the reformulation and reorientation of nursing curricula, restraining deficits, and optimizing the potentialities.

"EMPODEREENF": construction of an application for nurses' continuing education on psychological violence against women / "EMPODEREENF": construcción de una aplicación para la educación continua de enfermeras sobre violencia psicológica contra la mujer / "EMPODEREENF": construção de aplicativo para educação permanente de enfermeiros sobre violência psicológica contra a mulher

ABSTRACT Objective: to construct a prototype of a mobile application on psychological violence against women to facilitate nurses' continuing education. Method: an applied methodological research of technological development, based on the Contextualized Instructional Design method. Results: the prototype content was based on the learning objectives developed through a narrative review, which guided an integrative review to compile the information. The prototype is called "EmpodereEnf", and is composed of an initial screen, bringing nurses as a target audience, and, soon after, offering nine general moblets for access to information such as: concepts, causes, manifestations and consequences of psychological violence; means for identification and approach in nursing consultation and health education; compulsory notification and referral; examples of cases of psychological violence and references. Final considerations: the prototype is a possibility for future coping interventions and a work tool for nurses in the face of psychological violence against women.

RESUMEN Objetivo: construir un prototipo de aplicación móvil sobre violencia psicológica contra la mujer para facilitar la formación continua de enfermeras. Método: investigación metodológica aplicada del desarrollo tecnológico, basada en el método del Diseño Instruccional Contextualizado. Resultados: el contenido del prototipo se basó en los objetivos de aprendizaje desarrollados a través de una revisión narrativa, que orientó una revisión integradora para recopilar la información. El prototipo se llama "EmpodereEnf", y consta de una pantalla inicial, con el enfermero como público objetivo, y, seguidamente, ofrece nueve moblets generales para acceder a información como: conceptos, causas, manifestaciones y consecuencias de la violencia psicológica; medios de identificación y abordaje en la consulta de enfermería y educación en salud; notificación y remisión obligatorias; ejemplos de casos de violencia psicológica y referencias. Consideraciones finales: el prototipo constituye una posibilidad para futuras intervenciones de afrontamiento y una herramienta de trabajo para las enfermeras frente a la violencia psicológica contra la mujer.

RESUMO Objetivo: construir um protótipo de aplicativo móvel sobre violência psicológica contra a mulher para facilitar a educação permanente de enfermeiros. Método: pesquisa metodológica aplicada de desenvolvimento tecnológico, baseada no método Design Instrucional Contextualizado. Resultados: o conteúdo do protótipo foi embasado pelos objetivos da aprendizagem elaborados por meio de uma revisão narrativa, os quais nortearam uma revisão integrativa para compilação das informações. O protótipo intitula-se "EmpodereEnf", e é composto por tela inicial, trazendo o enfermeiro como público-alvo, e, logo em seguida, oferta nove moblets gerais de acesso às informações como: conceitos, causas, manifestações e consequências da violência psicológica; meios para identificação e abordagem na consulta de enfermagem e educação em saúde; notificação compulsória e encaminhamento; exemplos de casos de violência psicológica e referências. Considerações finais: o protótipo constitui possibilidade para futuras intervenções de enfrentamento e instrumento de trabalho do enfermeiro frente à violência psicológica contra a mulher.

Nurse's competences in promoting women's health in light of the Galway Consensus / Las competencias del enfermero en la promoción de salud de la mujer a la luz del Consenso de Galway / Competências do enfermeiro na promoção da saúde da mulher à luz do Consenso de Galway

ABSTRACT Objectives: to identify nurses' competences in promoting women's health. Methods: descriptive study with a qualitative approach that adopted the Galway Consensus as a theoretical-methodological framework. Data collection was performed through semi-structured interviews. Results: most of the Galway Consensus' competence domains were present in the nurses' interventions related to health promotion in women's care. "Assessment of needs" and "Implementation" were the most highlighted domains, followed by "Leadership" and "Impact assessment". The domain "Defending/Advocating Rights" was not identified. Final Considerations: within the nurses' work with women, some of the Galway Consensus domains of competencies for health promotion were present. However, there is a need, in the context of continuous health education, to expand strategies and enhance the development and application of these health promotion competences.

RESUMEN Objetivos: identificar las competencias de enfermeros en la promoción de salud de la mujer. Métodos: estudio descriptivo con abordaje cualitativo que adoptó el Consenso de Galway como referencial teórico-metodológico. Recolecta de datos fue realizada por medio de entrevista semiestructurada. Resultados: mayoría de los dominios de competencias del Consenso de Galway fueron contemplados en las intervenciones del enfermero relacionadas a promoción de salud en el cuidado a la mujer. "Evaluación de las necesidades" e "Implementación" fueron los dominios más evidenciados, seguidos de "Liderazgo" e "Evaluación del impacto". No fue identificado el dominio "Defensa de derechos". Consideraciones Finales: en el trabajo de los enfermeros con las mujeres, hubo la presencia de algunos dominios de las competencias de promoción de salud del Consenso de Galway. Pero, hay necesidad, en el ámbito de la educación permanente en salud, de ampliar estrategias y potencializar el desarrollo y aplicación de esas competencias de promoción de salud.

RESUMO Objetivos: identificar as competências de enfermeiros na promoção da saúde da mulher. Métodos: estudo descritivo com abordagem qualitativa que adotou o Consenso de Galway como referencial teórico-metodológico. A coleta de dados foi realizada por meio de entrevista semiestruturada. Resultados: a maioria dos domínios de competências do Consenso de Galway foram contemplados nas intervenções do enfermeiro relacionadas à promoção da saúde no cuidado à mulher. "Avaliação das necessidades" e "Implementação" foram os domínios mais evidenciados, seguidos de "Liderança" e "Avaliação do impacto". Não foi identificado o domínio "Defesa de direitos". Considerações Finais: no trabalho dos enfermeiros com as mulheres, houve a presença de alguns domínios das competências de promoção da saúde do Consenso de Galway. Porém, há necessidade, no âmbito da educação permanente em saúde, de ampliar estratégias e potencializar o desenvolvimento e aplicação dessas competências de promoção da saúde.

Saúde LGBTQIA+ à luz da bioética principialista / LGBTQIA+ health in light of principlist bioethics / Salud LGBTQIA+ a la luz de la bioética principialista

Resumo O artigo analisa, à luz da bioética principialista, a experiência de lésbicas, gays, bissexuais, travestis e transexuais, queers, intersexuais, assexuais e outras identidades de gênero e orientações sexuais no acesso a serviços de saúde. Trata-se de pesquisa transversal, de abordagem qualitativa, realizada em 2018, no município de Iguatu/CE, Brasil, com 26 pessoas lésbicas, gays, bissexuais e transexuais, que participaram de grupos focais com entrevistas semiestruturadas. Os resultados apontam graves violações aos princípios básicos da corrente principialista, com violência institucional de cunho psicológico, desrespeito à dignidade, recusa de doação de sangue, não aceitação de nome social, falta de acolhimento e humanização do atendimento. A persistência e a gravidade das situações de discriminação relatadas mostram que, apesar dos avanços e da existência de uma política de saúde específica para essa população, as medidas de combate ao preconceito institucional têm se mostrado insuficientes.

Abstract In light of principlist bioethics, this article analyzes the experiences of lesbian, gay, bisexual, travesti and transgender, queer, intersex, asexual and other gender identities and sexual orientations regarding access to health services. Conducted in 2018, in the municipality of Iguatu, Ceará, Brazil, this cross-sectional, qualitative research conducted semi-structured interviews with 26 lesbian, gay, bisexual and transgender individuals who participated in focus groups. Results reveal serious violations of basic bioethical principles, including institutional violence of psychological nature, disrespect to dignity, refusal of blood donation, non-acceptance of social name, lack of receptive and humanized care. The persistence and severity of the reported situations of discrimination show that, despite the progress and the existence of a specific health policy for this population, the measures to combat institutional prejudice have proven insufficient.

Resumen El artículo analiza, a la luz de la bioética principialista, la experiencia de lesbianas, gays, bisexuales, transexuales y travestis, queers intersexuales, asexuales y otras identidades de género y orientaciones sexuales en el acceso a los servicios de salud. Se trata de una investigación transversal, con enfoque cualitativo, realizada en 2018, en el municipio de Iguatu/CE, Brasil, con 26 personas lesbianas, gays, bisexuales y transexuales, que participaron en grupos focales con entrevistas semiestructuradas. Los resultados indican graves violaciones de los principios básicos de la corriente principialista, con violencia institucional de carácter psicológico, desprecio por la dignidad, negativa a donar sangre, no aceptación del nombre social, falta de recepción y humanización del cuidado. La persistencia y gravedad de las situaciones de discriminación denunciadas muestran que, a pesar de los avances y la existencia de una política de salud específica para esta población, las medidas para combatir el prejuicio institucional han resultado insuficientes.

Competencies in health promotion by nurses for adolescents.

OBJECTIVE: to describe the health promotion competency domains, performed by nurses, for adolescents, according to the Galway Consensus. METHOD: a qualitative study based on the Galway Consensus theoretical methodological framework. Fifteen nurses from northeastern Brazil participated. Data were collected between April and May 2017 through pre-structured interviews, submitted to the content analysis technique and analyzed according to the Galway Consensus dimensions. RESULTS: the following competency areas were found: catalyzing changes, leadership, needs assessment, planning, implementation, and partnerships. These competencies were contemplated from embracement of adolescents at health unit, guidance, teamwork, educational activities and lectures, as well as active search. CONCLUSION: most health promotion domains were observed; however, it is still suggested that there are challenges to an effective performance of health promotion among adolescents because some competency domains in health promotion have not been evidenced.

Computational Resources and Strategies to Construct Single-Molecule Models of FISH.

Currently, the interactions occurring between oligonucleotides and the cellular envelope of bacteria are not fully resolved at the molecular level. Understanding these interactions is essential to gain insights on how to improve the internalization of the tagged oligonucleotides during fluorescence in situ hybridization (FISH). Agent-based modeling (ABM) is a promising in silico tool to dynamically simulate FISH and bring forward new knowledge on this process. Notably, it is important to simulate the whole bacterial cell, including the different layers of the cell envelope, given that the oligonucleotide must cross the envelope to reach its target in the cytosol. In addition, it is also important to characterize other molecules in the cell to best emulate the cell and represent molecular crowding. Here, we review the main information that should be compiled to construct an ABM on FISH and provide a practical example of an oligonucleotide targeting the 23S rRNA of Escherichia coli .

Computational resources and strategies to assess single-molecule dynamics of the translation process in S. cerevisiae.

This work provides a systematic and comprehensive overview of available resources for the molecular-scale modelling of the translation process through agent-based modelling. The case study is the translation in Saccharomyces cerevisiae, one of the most studied yeasts. The data curation workflow encompassed structural information about the yeast (i.e. the simulation environment), and the proteins, ribonucleic acids and other types of molecules involved in the process (i.e. the agents). Moreover, it covers the main process events, such as diffusion (i.e. motion of molecules in the environment) and collision efficiency (i.e. interaction between molecules). Data previously determined by wet-lab techniques were preferred, resorting to computational predictions/extrapolations only when strictly necessary. The computational modelling of the translation processes is of added industrial interest, since it may bring forward knowledge on how to control such phenomena and enhance the production of proteins of interest in a faster and more efficient manner.

Competencies in health promotion by nurses for adolescents / Competências na promoção da saúde de enfermeiros a adolescentes / Competencias en promoción de la salud de enfermeras a adolescentes

ABSTRACT Objective: to describe the health promotion competency domains, performed by nurses, for adolescents, according to the Galway Consensus. Method: a qualitative study based on the Galway Consensus theoretical methodological framework. Fifteen nurses from northeastern Brazil participated. Data were collected between April and May 2017 through pre-structured interviews, submitted to the content analysis technique and analyzed according to the Galway Consensus dimensions. Results: the following competency areas were found: catalyzing changes, leadership, needs assessment, planning, implementation, and partnerships. These competencies were contemplated from embracement of adolescents at health unit, guidance, teamwork, educational activities and lectures, as well as active search. Conclusion: most health promotion domains were observed; however, it is still suggested that there are challenges to an effective performance of health promotion among adolescents because some competency domains in health promotion have not been evidenced.

RESUMO Objetivo: descrever os domínios das competências da promoção da saúde, realizados por enfermeiros, para com adolescentes, segundo o Consenso de Galway. Método: estudo qualitativa à luz do Consenso de Galway. Participaram 15 enfermeiros de um município da Região Nordeste, Brasil. Os dados foram coletados entre abril e maio de 2017, por meio de entrevista semiestruturada, submetidos à técnica de análise de conteúdo e analisados segundo as dimensões de Consenso de Galway. Resultados: evidenciaram-se os seis domínios do Consenso. Essas competências foram contempladas a partir da recepção do adolescente na unidade de saúde, orientações, trabalho em equipe, realização de ações educativas e palestras, e busca ativa. Considerações finais: a maioria dos domínios de promoção da saúde foram contemplados. No entanto, ainda sugere-se que ainda há desafios para o desempenho efetivo da promoção da saúde junto ao adolescente, pois alguns domínios da competência de promoção da saúde não foram evidenciados.

RESUMEN Objetivo: describir los dominios de las competencias de promoción de la salud, realizadas por enfermeras, para adolescentes, según el Consenso de Galway. Método: estudio cualitativo a la luz del Consenso de Galway. Participaron quince enfermeras de un municipio de la Región Nordeste, Brasil. Los datos fueron recolectados entre abril y mayo de 2017, mediante entrevistas semiestructuradas, sometidos a la técnica de análisis de contenido y analizados según las dimensiones del Consenso de Galway. Resultados: se evidenciaron los seis dominios del Consenso. Estas habilidades fueron contempladas desde la recepción del adolescente en la unidad de salud, la orientación, el trabajo en equipo, las actividades educativas y conferencias, y la búsqueda activa. Consideraciones finales: se cubrieron la mayoría de los dominios de promoción de la salud. Sin embargo, todavía se sugiere que aún existen desafíos para el desempeño efectivo de la promoción de la salud entre los adolescentes, ya que no se han evidenciado algunos dominios de competencia en la promoción de la salud.

A comprehensive model for the diffusion and hybridization processes of nucleic acid probes in fluorescence in situ hybridization.

Fluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main limiting steps for the hybridization to occur remain unclear. In here, FISH is approached as a particular case of a diffusion-reaction kinetics, where molecular probes (MPs) move from the hybridization solution to the target RNA site within the cells. Based on literature models, the characteristic times taken by different MPs to diffuse across multiple cellular barriers, as well as the reaction time associated with the formation of the duplex molecular probe-RNA, were estimated. Structural and size differences at the membrane level of bacterial and animal cells were considered. For bacterial cells, the limiting step for diffusion is likely to be the peptidoglycan layer (characteristic time of 7.94 × 102 - 4.39 × 103 s), whereas for animal cells, the limiting step should be the diffusion of the probe through the bulk (1.8-5.0 s) followed by the diffusion through the lipid membrane (1 s). The information provided here may serve as a basis for a more rational development of FISH protocols in the future.

Exploring the Brazilian diversity of Aspergillus sp. strains for lovastatin and itaconic acid production.

Filamentous fungi are well known for producing secondary metabolites applied in various industrial segments. Among these, lovastatin and itaconic acid, produced by Aspergillus terreus, have applications in the pharmaceutical and chemical industries. Lovastatin is primarily used for the control of hypercholesterolemia, while itaconic acid is a building block for the production of synthetic fibers, coating adhesives, among others. In this study, for the first time, 35 strains of Aspergillus sp. from four Brazilian culture collections were evaluated for lovastatin and itaconic acid production and compared to a reference strain, ATCC 20542. From an initial screening, the strains ATCC 20542, URM 224, URM1876, URM 5061, URM 5254, URM 5256, URM 5650, and URM 5961 were selected for genomic comparison. Among tested strains, the locus corresponding to the lovastatin genomic cluster was assembled, showing that all genes essential for lovastatin biosynthesis were present in producing URM 5961 and URM 5650 strains, with 100% and 98.5% similarity to ATCC 20542, respectively. However, in the no producing URM 1876, URM 224, URM 5254, URM 5061, and URM 5256 strains, this cluster was either fragmented or missing. Among the 35 strains evaluated for itaconic acid production in this study, only three strains had titers above 0.5 g/L, 16 strains had production below 0.5 g/L, and the remaining 18 strains had no production, with the highest production of itaconic acid observed in the URM 5254 strain with 2.2 g/L. The essential genes for itaconic acid production, mttA, cadA msfA were also mapped, where all three genes linked to itaconic acid production were found in a single contig in the assembly of each strain. In contrast to lovastatin loci, there is no correlation between the level of itaconic acid production and genetic polymorphisms in the genes associated with its biosynthesis.

Síndrome de Burnout em uma unidade hospitalar: percepções da equipe de enfermagem / Burnout syndrome in a hospital unit: perceptions of the nursing team / Síndrome de Burnout en una unidad hospitalaria: percepciones del equipo de enfermería

Objetivo: Conhecer a percepção da equipe de enfermagem acerca da Síndrome de Burnout. Método: Estudo descritivo e exploratório com abordagem qualitativa, realizado com 24 profissionais de Enfermagem em uma unidade hospitalar, da região Centro Sul, Estado do Ceará - Brasil. Os dados foram coletados nos meses de setembro a outubro de 2017, por meio de entrevista semiestruturada e organizados em categorias temáticas. O estudo foi aprovado pelo Comitê de Ética em Pesquisa CAAE nº 69921117.3.0000.5055. Resultados: Emergiram duas categorias: Concepção dos profissionais acerca de seu trabalho de enfermagem e Percepção da equipe de enfermagem acerca da síndrome de Burnout. Conclusão: A equipe de enfermagem compreende, de forma frágil, a síndrome de Burnout, sendo necessário educação em saúde para os profissionais, visando identificação dessa síndrome para melhoria da qualidade de vida no trabalho

Objective: To know the nursing team's perception about Burnout Syndrome. Method: Descriptive and exploratory study with a qualitative approach, carried out with 24 nursing professionals in a hospital unit, in the Centro Sul region, State of Ceará, Brazil. Data were collected from September to October 2017, through semi-structured interviews, and organized into thematic categories. The study was approved by the Ethics Committee in Research CAAE nº 69921117.3.0000.5055. Results: Two categories emerged: Conception of professionals about their nursing work and Perception of the nursing team about Burnout syndrome. Conclusion: The nursing team comprehends, in a fragile way, Burnout syndrome, requiring health education for professionals, aiming at identification and treatment, culminating in the improvement of quality of life at work

Objetivo: Conocer la percepción del equipo de enfermería acerca del Síndrome de Burnout. Método: Estudio descriptivo y exploratorio con abordaje cualitativo, realizado con 24 profesionales de Enfermería en una unidad hospitalaria, de la región Centro Sur, Estado de Ceará-Brasil. Los datos fueron recolectados en los meses de septiembre a octubre de 2017, por medio de entrevista semiestructurada, y organizados en categorías temáticas. El estudio fue aprobado por el Comité de Ética en Investigación CAAE nº 69921117.3.0000.5055. Resultados: Emergieron dos categorías: Concepción de los profesionales acerca de su trabajo de enfermería y Percepción del equipo de enfermería acerca del síndrome de Burnout. Conclusión: El equipo de enfermería comprende, de forma frágil, el síndrome de Burnout, siendo necesaria educación en salud para los profesionales, buscando identificación y tratamiento, culminando en la mejora de la calidad de vida en el trabajo

Host-defense peptides AC12, DK16 and RC11 with immunomodulatory activity isolated from Hypsiboas raniceps skin secretion.

Inflammation is a natural defense mechanism of the immune system; however, when unregulated, it can lead to chronic illness. Glucocorticoids are the most commonly used agents to effectively treat inflammatory conditions, including autoimmune diseases, however these substances can trigger a number of side effects. Thus, viable alternatives to the use of these drugs would be advantageous. In this study, we have analyzed the anti-inflammatory profile of three synthetic peptides first identified in skin secretion of the tree frog Hypsiboas raniceps. Structural characterization was performed using NMR spectroscopy and Mass Spectrometry, and the peptides were tested in vitro in RAW 264.7 cells and in vivo in Balb/c mice for their functional properties. The samples did not show a significant antimicrobial profile. NMR spectroscopy indicated that AC12 (ACFLTRLGTYVC) has a disulfide bond between C2 and C11 and a ß-sheet-turn-ß-sheet conformation in aqueous solution. This peptide showed no cytotoxic effect in mammalian cells and it was the most effective in reducing anti-inflammatory markers, such as NO, TNF-α and IL-12. Peptide DK16 (DKERPICSNTFRGRKC) demonstrated anti-inflammatory properties in vitro, while RC11 (RCFRRRGKLTC) significantly altered the cell viability in RAW 264.7 but was shown to be safe in Balb/c erythrocytes. Our results indicate that, of the three peptides studied, AC12 is the most efficient in reducing anti-inflammatory markers, and it could be a potential agent for the treatment of inflammatory diseases.

Reviewing Mechanistic Peptidomics in Body Fluids Focusing on Proteases.

The comprehension of how protease networks sculpt proteomes might help to disclose the functional annotation of the peptidome in health and disease. Envisioning to add new insights on the protease networks involved in the regulation of body fluid peptidomes, the authors apply Proteasix software to predict the proteases involved in the generation of the naturally occurring peptides present in six of the most studied human body fluids. Peptidome data is collected from the databases and from experimental studies. The analysis highlights 132 putative proteases from four families with the predominance of serine proteases and metalloproteases. From these, 49 proteases seem to be common to all fluids and are mostly associated to extracellular matrix organization as well as protein/peptide hormone processing. Data analysis also emphasizes: i) the similarity between plasma and CSF protease profiles; ii) that saliva and tears share proteases involved in the generation of peptides with antimicrobial activity; iii) that urine is the body fluid with the highest number of unique putative proteases, precluding an easy tracing of proteolytic events in this case. Taken together, the analysis emphasizes the intricate modus operandi of proteases, challenged by the interconnected pathways and amplification cascades in which they are involved.

Draft Genome Sequence of the Antimicrobial-Producing Strain Paenibacillus elgii AC13.

A Paenibacillus elgii strain isolated from soil samples from Cerrado, Brazil, showed antimicrobial activity. Its genome sequence was acquired (GS20 FLX Titanium 454 platform) and comprises 108 contigs (N50, 198,427 bp) and 6,810 predicted sequences. Here, we shed some light on the antimicrobial genes of the strain, including a nonribosomal peptide synthetase (NRPS) module identified as part of a pelgipeptin gene cluster.

How to use and integrate bioinformatics tools to compare proteomic data from distinct conditions? A tutorial using the pathological similarities between Aortic Valve Stenosis and Coronary Artery Disease as a case-study.

Nowadays we are surrounded by a plethora of bioinformatics tools, powerful enough to deal with the large amounts of data arising from proteomic studies, but whose application is sometimes hard to find. Therefore, we used a specific clinical problem - to discriminate pathophysiology and potential biomarkers between two similar cardiovascular diseases, aortic valve stenosis (AVS) and coronary artery disease (CAD) - to make a step-by-step guide through four bioinformatics tools: STRING, DisGeNET, Cytoscape and ClueGO. Proteome data was collected from articles available on PubMed centered on proteomic studies enrolling subjects with AVS or CAD. Through the analysis of gene ontology provided by STRING and ClueGO we could find specific biological phenomena associated with AVS, such as down-regulation of elastic fiber assembly, and with CAD, such as up-regulation of plasminogen activation. Moreover, through Cytoscape and DisGeNET we could pinpoint surrogate markers either for AVS (e.g. popeye domain containing protein 2 and 28S ribosomal protein S36, mitochondrial) or for CAD (e.g. ankyrin repeat and SOCS box protein 7) which deserve future validation. Data recycling and integration as well as research orientation are among the main advantages of resorting to bioinformatics analysis, hence these tutorials can be of great convenience for proteomics investigators. BIOLOGICAL SIGNIFICANCE: As we saw for aortic valve stenosis and coronary artery disease, it can be of great relevance to perform preliminary bioinformatics analysis with already published proteomics data. It not only saves us time in the lab (avoiding work duplication) as it points out new hypothesis to explain the phenotypical presentation of the diseases as well as new surrogate markers with clinical relevance, deserving future scrutiny. These essential steps can be easily overcome if one follows the steps proposed in our tutorial for STRING, DisGeNET, Cytoscape and ClueGO utilization.