Identification of sulfur-oxidizing bacteria from fishponds and their performance to remove hydrogen sulfide under aquarium conditions.

Hydrogen sulfide is a highly toxic gas that causes many economic losses in aquaculture ponds. The application of sulfur-oxidizing bacteria (SOB) to remove hydrogen sulfide is an eco-friendly approach. This study aimed to isolate and identify the most efficient SOBs from the sediment of warm-water fish farms. Enrichment and isolation were performed in three different culture media (Starkey, Postgate, and H-3) based on both mineral and organic carbon. Overall, 27 isolates (14 autotrophic and 13 heterotrophic isolates) were purified based on colony and cell morphology differences. Initial screening was performed based on pH decrease. For final screening, the isolates were assessed based on their efficacy in thiosulfate oxidation and the sulfate production on Starkey liquid medium. Among isolated strains, 3 strains of Iran 2 (FH-13), Iran 3 (FH-21), and Iran 1 (FH-14) that belonged to Thiobacillus thioparus species (identified by 16s rRNA) showed the highest ability in thiosulfate oxidation (413.21, 1362.50, and 4188.03 mg/L for 14 days) and the highest sulfate production (3350, 2075, and 1600 mg/L). In the final phase, the performance of these strains under aquarium conditions showed that Iran 1 and Iran 2 had the highest ability in sulfur oxidation. In conclusion, Iran 1 and 2 strains can be used as effective SOB to remove hydrogen sulfide in fish farms. It is very important to evaluate strains in an appropriate strategy using a combination of different criteria to ensure optimal performance of SOB in farm conditions.

Screening and evaluation of phosphate-solubilizing bacteria isolated from aquaculture ponds in a step-by-step strategy as potential biofertilizer.

AIMS: The application of phosphate-solubilizing bacteria (PSB) has received little attention in aquaculture. In addition, the low efficiency of PSB as a biofertilizer in farm conditions is a major concern. Therefore, this study aims to isolate the PSB from sediment of earthen fishponds and evaluate with a more appropriate approach to ensure their effectiveness in increasing the bioavailability of phosphorus (P) in farm conditions. METHODS AND RESULTS: PSB was first isolated and selected using the National Botanical Research Institute's Phosphate (NBRIP) medium-containing tri-calcium phosphate (TCP) in solid and liquid media. Among 96 strains that were isolated, 11 strains identified by 16 s rRNA, belonging to the genera Pseudomonas and Acinetobacter, showed a higher ability to release P from TCP (48-170 mg L-1 ). Then, the efficiency of 11 strains was evaluated by combining different criteria. Among 11 selected strains, based on the ability to dissolve TCP and Ca-Phytate in culture medium, release P in sediment microcosm, and growth in a wide range of environmental conditions in fishponds, especially optimum growth at 4°C and pH above 8, Ps. deceptionensis strain Persian10 was selected as the most efficient strain for testing in aquarium conditions. In the last step, incubation of Persian10 in aquarium sediment (sterilized under gamma-ray) increased soluble P and had an impact on calcium phosphate, organic P and alkaline phosphatase activity; however, Persian10 had no impact on the concentration of iron phosphate and aluminium phosphate. CONCLUSIONS: Persian 10 strain can be considered a bio-fertilizer candidate in earthen fishponds as it expressed the ability to solubilize P in different conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: It is very important to evaluate PSB in an appropriate process using a combination of different criteria to ensure optimal performance of PSB in farm conditions.

Ecosystem services trade-offs informing impacts of marine aquaculture development in the southern Caspian Sea.

Humans have always benefited from marine ecosystems and the use of their services has increased over time. The principal challenge of managing ecosystem services is that they are not independent of each other. Attempts to optimize a single service have often led to the reductions or losses of other services; in other words, they are "traded-offs". Based on the purpose of assessing the impacts of marine aquaculture development in the Southern Caspian Sea, 3 management scenarios were utilized to model 3 categories, including marine aquaculture, Water Quality (WQ) and Habitat Risk Assessment (HRA) through marine Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST). Marine aquaculture model was first considered for Oncorhynchus mykiss that the results were as follows: in the baseline scenario with 9 farms, the amounts of the total weight of fish produced and Net Present Value (NPV) measured during 1 year equaled 1970 tons and 2,247,000 dollars; in the conservation scenario with 4 farms, their amounts were 800 tons and 1 million dollars; in the expansion scenario with an increased number of farms (20 farms), their amounts were estimated to be 4000 tons and 5 million dollars, respectively. The results of the marine aquaculture model were utilized as the inputs both to WQ (amount of BOD released from each farm) and HRA (habitats of four species of fish (Rutilus frisii kutum, Acipenser stellatus, Acipenser persicus, and Huso huso) models. The results revealed 41%, 16%, and 60% of WQ reductions and 35%, 20%, and 45% of HRA changes in the baseline, conservation, and expansion scenarios, respectively. Considering the current production of 9 existing farms (1970 tons per year) and the impact of this production on water quality (41%) and habitat risk (35%), it is recommended to reduce this production volume and doing aquaculture development in the Southern Caspian Sea without considering and managing negative consequences can have dangerous consequences for this sensitive ecosystem.

Serum biochemical and non-specific immune responses of rainbow trout (Oncorhynchus mykiss) to dietary nucleotide and chronic stress.

The aim of the present study was to investigate whether supplementary nucleotide "Optimun" mitigates the adverse effects of chronic overcrowding in Oncorhynchus mykiss. Two experimental diets [control and nucleotide-supplemented (0.2 %)] and two rearing densities (10 and 30 kg m(-3)) were combined to have four experimental treatments. The fish were reared for 45 days under different densities using different diets. At the end of the trial, FCR of the fish in higher density was significantly higher than those of the lower density. Nucleotide had no significant effects on growth performance and survival rate. Supplemented nucleotide significantly increased blood hematocrit, whereas it decreased serum total protein, total immunoglobulin (Ig) and creatinine. Overcrowding significantly increased serum glucose and total protein level and decreased serum lysozyme activity, but supplemented nucleotide produced no improvement in these items. No significant effect of overcrowding and dietary nucleotide was observed on serum cortisol. Supplemented nucleotide significantly increased serum urea under low stocking density. Overall, the results showed that 0.2 % "Optimun" had no positive effects on rainbow trout and also caused some immunological and metabolic problems. These findings are not in accordance with those obtained in the same species, with same nucleotide source and level, but acute stress; thus, further studies are encouraged on this topic.

Effects of dietary nucleotides supplementation on rainbow trout (Oncorhynchus mykiss) performance and acute stress response.

This experiment was conducted to examine the effect of dietary nucleotides (NT) on fish performance and acute stress response on fingerling rainbow trout (23 g ± 0.01, mean weight ± SEM). Five experimental diets according to different levels of supplemented nucleotides (0, 0.05, 0.1, 0.15, and 0.2%) were assayed on experimental fish for 8 weeks. Growth, hematological parameters (hematocrit, hemoglobin, erythrocyte, lymphocyte, and neutrophil count), serum proteins (globulin, albumin), and plasma enzymatic activity (alkaline phosphatase, ALP; aspartate transaminase, AST; lactate dehydrogenase, LDH; alanine transaminase, ALT) were assayed. At the end of feeding trial, fish fed the control and 0.2% diets were subjected to handling and crowding stress. Modulatory effects of nucleotides on acute stress response (cortisol and glucose) and plasma electrolytes (Na(+), Cl(-), K(+), and Ca(2+)) were studied. The percentage of body weight gain (WG) and feed efficiency (FE) of fish were better when the fish were fed 0.15-0.2% diets. Fish fed the nucleotide-supplemented diets tended to have lower levels of serum enzymes including ALP, AST, LDH, and ALT. Plasma cortisol levels of fish fed on 0.2% diet under handling and crowding stress were significantly lower than fish fed the control diet at all post-stress time intervals. In our study, fish fed nucleotide-supplemented diet had significantly lower concentrations of glucose compared to those fed the basal diet. The concentrations of sodium, chloride, calcium, and potassium of fish fed the control diet were significantly lower than in fish fed nucleotide-supplemented diet. Dietary nucleotides administration seems to promote growth and to enhance resistance against handling and crowding stress in fingerling rainbow trout.

Dietary administration of nucleotides to enhance growth, humoral immune responses, and disease resistance of the rainbow trout (Oncorhynchus mykiss) fingerlings.

A feeding study was conducted to determine the effect of dietary nucleotides (NT) on growth performance, immune responses and resistance of rainbow trout fingerlings to Streptococcus iniae. A basal diet supplemented with 0 (control), 0.5, 1, 1.5 and 2 g NT kg(-1) to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish with initial average weight of approximately 23 g. After 8 weeks of feeding trial, fish fed diets with 1.5-2 g NT kg(-1) had the highest final weight and SGR, followed by groups fed the diets with 0.5-1 g NT kg(-1), and lowest in fish fed the NT-free control diet. The feed conversion ratio (FCR) was better when the fish were fed diet with 2 g NT kg(-1). The ACH(50) level and lysozyme activity in serum were found to be significantly (P < 0.05) greater in fish fed nucleotides at 1.5-2 g kg(-1). The fish fed with 2 g NT kg(-1) had higher IgM level. The challenge experiment showed that relative percent survival (RPS) increased significantly (P < 0.05) with increasing levels of nucleotides from 0.5 to 2 g kg(-1) and was lowest in the control group. We therefore recommend dietary nucleotide administration at 1.5-2 g kg(-1) to promote growth and enhance immunity and resistance against S. iniae.