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1.
Eur J Clin Microbiol Infect Dis ; 34(9): 1787-95, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26054715

RESUMO

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > 10(5) CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs.


Assuntos
Bacteriúria/diagnóstico , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Urinálise/métodos , Infecções Urinárias/diagnóstico , Automação Laboratorial , Técnicas de Tipagem Bacteriana , Humanos , Sensibilidade e Especificidade , Infecções Urinárias/microbiologia
2.
J Food Prot ; 71(7): 1372-8, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18680935

RESUMO

Enterobacter sakazakii is regarded as a ubiquitous organism that can be isolated from a wide range of foods and environments. Infection in at-risk infants has been epidemiologically linked to the consumption of contaminated powdered infant formula. Preventing the dissemination of this pathogen in a powdered infant formula manufacturing facility is an important step in ensuring consumer confidence in a given brand together with the protection of the health status of a vulnerable population. In this study we report the application of a repetitive sequence-based PCR typing method to subtype a previously well-characterized collection of E. sakazakii isolates of diverse origin. While both methods successfully discriminated between the collection of isolates, repetitive sequence-based PCR identified 65 types, whereas pulsed-field gel electrophoresis identified 110 types showing > or =95% similarity. The method was quick and easy to perform, and our data demonstrated the utility and value of this approach to monitor in-process contamination, which could potentially contribute to a reduction in the transmission of E. sakazakii.


Assuntos
Cronobacter sakazakii/isolamento & purificação , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Reação em Cadeia da Polimerase/métodos , Contagem de Colônia Microbiana/métodos , Qualidade de Produtos para o Consumidor , Cronobacter sakazakii/classificação , Eletroforese em Gel de Campo Pulsado , Contaminação de Alimentos/prevenção & controle , Humanos , Lactente , Fórmulas Infantis , Filogenia , Análise de Sequência de DNA
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