Effect of NaCl, copper and cadmium ions on halophytes with different types of salt resistance: accumulation, physiological and biochemical reactions.

The capacities of the euhalophyte SaLi-Cornia perennans Willd. and glycohalophyte Artemisia santonica L. to accumulate NaCl, Cu, and Cd, as well as their physiological and biochemical responses to these compounds, was investigated. Seeds were germinated in distilled water for 1-3 days and then sown in containers with sand. Plants were watered with Robinson's nutrient solution. After 3 months, plants were divided into two groups: experimental and control. In the experimental group, soil was treated with 1M NaCl, 10 mM Cu(NO3)2, and 10 mM Cd(NO3)2 for 24 h. The exposure to high concentration of NaCl in the experiment did not affect the baseline level of Na, which was twice as high in S. perennans as in A. santonica. Plant exposure to Cu and Cd caused their accumulation in the aboveground parts of both species. The accumulation capacity of the euhalophyte was many times higher than that of the glycohalophyte. We analysed functional parameters of leaves by measuring photosynthetic pigments, structural parameters of membranes by assessing the lipid profile, and the balance of pro/antioxidant processes. Using data on changes in several biochemical parameters, the sensitivity of the two different halophytes to metal ions was as follows: for S. perennans - Cu > Na > Cd; for A. santonica - Na > Cu > Cd. Our findings suggest that S. perennans can be used for heavy metal extraction from soil in phytoremediation, whereas A. santonica will be more effective for greening of polluted territories.

Tuberculosis cases caused by heterogeneous infection in Eastern Europe and their influence on outcomes.

INTRODUCTION: Mycobacterium tuberculosis superinfection is known to occur in areas with high rates of tuberculosis (TB) and has a significant impact on overall clinical TB management. AIM: We aimed to estimate the superinfection rate in cohorts of drug sensitive and multi-drug resistant tuberculosis (MDR TB) patients from Eastern Europe and the potential role of a second MDR TB strain infecting a patient with active non-MDR TB in treatment outcome. METHODS: The study population included 512 serial M. tuberculosis isolates obtained from 84 MDR- and 136 non-MDR TB patients recruited sequentially at sites in Lithuania, Latvia and Russia in 2011-2013. Strains were genotyped using standardized 24-loci Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing. RESULTS: Changes in two or more MIRU-VNTR loci suggesting superinfection were detected in 13 patients (5.9%). We found 4 initially non-MDR TB patients superinfected with an MDR TB strain during treatment and 3 of them had an unsuccessful outcome. CONCLUSIONS: An unsuccessful treatment outcome in patients initially diagnosed with drug sensitive TB might be explained by superinfection with an MDR TB strain. Bacteriological reversion could be indicative of superinfection with another strain. Archiving of all serial isolates and their genotyping in case of culture reversion could support therapeutic strategies in high MDR TB burden settings if resources are available.