RESUMO
BACKGROUND: The most common type of salivary gland tumor is pleomorphic adenoma. The genetic area of focus in the diagnosis of salivary gland tumors is a study of the role of miRNA. METHODS: Twenty-two patients with pleomorphic adenomas of the salivary glands were used for the examinations. The histological typing of the salivary gland tumors was performed when using routine staining with hematoxylin and eosin, as well as with immunohistochemistry. The expressions of miR-34a and miR-29a were evaluated by using reverse transcription and the quantitative polymerase chain reaction in a real-time setting. In addition, the study also calculated the levels of expression of miR-29a and miR-34a in the venous blood. RESULTS: The majority of patients-15 (68.18%) and 22 (100.00%) had a positive response to human papillomavirus (HPV) and pleomorphic adenoma gene 1 (PLAG1), respectively. The conducted analyses of the expressions of miR-34a and miR-29a showed that the highest expression was observed in the salivary gland tissue adjacent to the tumor (1,052.02±367.20 and 111.93±56.97, versus 47.72±28.93 and 8.12±4.40 in the intact salivary gland tissue, respectively). CONCLUSIONS: There was a sufficiently high level of miR-34a and miR-29a expressions in the tissues of the tumor of pleomorphic adenomas of the salivary glands when compared with the intact salivary gland tissue.
RESUMO
PURPOSE: The aim was to identify the main risk factors for infection in patients with mandibular fractures located in the tooth-bearing area. MATERIAL AND METHODS: Seven hundred and eighty-nine case histories were retrospectively reviewed regarding treatment results and nature of complications developed. The data concerning possible risks were analysed statistically to find out those factors that seemed to have a significant influence on the development of infection. RESULTS: The incidence of infection in patients with mandibular fractures located in the tooth-bearing area was determined by the following risk factors: social and organizational conditions of medical care, trauma pattern, preexisting medical status and treatment tactics. The main contributory factors were delayed medical care, accompanying pathological disorders, angular location, multiple and comminuted fractures and the type of antibiotic used.