Evaluating the effectiveness of drones for quantifying invasive upside-down jellyfish (Cassiopea sp.) in Lake Macquarie, Australia.

Upside-down jellyfish (Cassiopea sp.) are mostly sedentary, benthic jellyfish that have invaded estuarine ecosystems around the world. Monitoring the spread of this invasive jellyfish must contend with high spatial and temporal variability in abundance of individuals, especially around their invasion front. Here, we evaluated the utility of drones to survey invasive Cassiopea in a coastal lake on the east coast of Australia. To assess the efficacy of a drone-based methodology, we compared the densities and counts of Cassiopea from drone observations to conventional boat-based observations and evaluated cost and time efficiency of these methods. We showed that there was no significant difference in Cassiopea density measured by drones compared to boat-based methods along the same transects. However, abundance estimates of Cassiopea derived from scaling-up transect densities were over-inflated by 319% for drones and 178% for boats, compared to drone-based counts of the whole site. Although conventional boat-based survey techniques were cost-efficient in the short-term, we recommend doing whole-of-site counts using drones. This is because it provides a time-saving and precise technique for long-term monitoring of the spatio-temporally dynamic invasion front of Cassiopea in coastal lakes and other sheltered marine habitats with relatively clear water.

Marine heatwaves have minimal influence on the quality of adult Sydney rock oyster flesh.

Marine heatwaves (MHWs) are impacting marine biodiversity, including fisheries and aquaculture. However, it is largely unknown which species will be able to endure MHWs and at what price. Here, we applied elevated temperature (2 °C above ambient) and two different heatwave scenarios to adults of the economically important Sydney rock oyster (SRO, Saccostrea glomerata), and evaluated the impact on nutritional properties, gene expression profiles and immune health indicators. We found that elevated temperature (23 °C) and a variable heatwave (VHW) during winter caused some significant differences in the micronutrient and trace elements levels in oyster flesh. There was an increase of lead under VHW and a decrease in chromium, barium and aluminium under elevated temperature. Conversely, gene expression profiles and other physiological parameters, including flesh protein, fatty acid profiles and hemocyte numbers, were not affected by MHWs. These results indicate that adult SRO are reasonably resilient, and should continue to provide high-quality seafood, under near-future ocean warming and moderate heatwave scenarios.

Conservation genomics of a critically endangered brown seaweed.

Seaweeds provide valuable ecosystem services, but many are undergoing global decline due to climate and anthropogenic stressors. The brown macroalga, Nereia lophocladia (hereafter called Nereia), is among only a handful of seaweeds globally to be listed as critically endangered and is only described from two known locations, but there exists little knowledge about this species. Here, we combine field surveys to verify the distribution of Nereia, with cutting-edge genomics to determine genetic diversity and population structure, and inform ongoing conservation actions. We expand Nereia's known distribution from one to seven locations along a 70-km long coastal stretch in New South Wales but reveal small population sizes at some sites (as few as 8 individuals despite extensive searching). A total of 1,261 genome-wide SNPs were retained from 70 individuals after filtering, and 304 outlier loci under putative selection were detected by one of three methods. Populations showed low genetic diversity (mean expected heterozygosity HE  = 0.055 ± 0.014) and high levels of inbreeding within populations (mean FIS  = 0.721 ± 0.085), along with high genetic differentiation among sites (mean FST  = 0.276), which may increase susceptibility to future environmental change and decrease the species' ability to recover after loss. Given these findings, we recommend the consideration of both in situ and ex situ conservation measures for Nereia, as well as further research into the species' ecology and biology. Nereia remains of conservation concern and its listing as critically endangered is justified until further investigation elucidates the full distribution and adaptive capacity of the species.

Resilience of a harvested gastropod, Turbo militaris, to marine heatwaves.

Marine heatwaves (MHW) are predicted to occur with increased frequency, duration and intensity in a changing climate, with pervasive ecological and socioeconomic consequences. While there is a growing understanding of the ecological impacts of warming and marine heatwaves, much less is known about how they influence the underlying physiology and health of species, and the nutritional properties of tissue. We evaluated the effects of different heatwave scenarios and ocean warming on the nutritional properties and immune health of the harvested gastropod Turbo militaris. Neither heatwave scenarios nor elevated temperatures had significant impacts on morphometrics, proximate composition or inorganic content of T. militaris. However, an increased moisture content and non-significant trends, such as elevated amount of lipids, and an increased number of hemocytes in the blood of T. militaris in the heatwave treatments were suggestive of mild stress. Overall, our study suggests that T. militaris is resilient to marine heatwaves and warming, although delayed, additive or synergistic stress responses cannot be ruled out. Understanding the possible effects of ocean warming and heatwaves on fisheries species could improve management actions to avoid species impacts, socioeconomic losses and negative effects to ecosystem service provision in a changing climate.

Invasion-mediated effects on marine trophic interactions in a changing climate: positive feedbacks favour kelp persistence.

The interactive effects of ocean warming and invasive species are complex and remain a source of uncertainty for projecting future ecological change. Climate-mediated change to trophic interactions can have pervasive ecological consequences, but the role of invasion in mediating trophic effects is largely unstudied. Using manipulative experiments in replicated outdoor mesocosms, we reveal how near-future ocean warming and macrophyte invasion scenarios interactively impact gastropod grazing intensity and preference for consumption of foundation macroalgae ( Ecklonia radiata and Sargassum vestitum). Elevated water temperature increased the consumption of both macroalgae through greater grazing intensity. Given the documented decline of kelp ( E. radiata) growth at higher water temperatures, enhanced grazing could contribute to the shift from kelp-dominated to Sargassum-dominated reefs that is occurring at the low-latitude margins of kelp distribution. However, the presence of a native invader ( Caulerpa filiformis) was related to low consumption by the herbivores on dominant kelp at warmer temperatures. Thus, antagonistic effects between climate change and a range expanding species can favour kelp persistence in a warmer future. Introduction of species should, therefore, not automatically be considered unfavourable under climate change scenarios. Climatic changes are increasing the need for effective management actions to address the interactive effects of multiple stressors and their ecological consequences, rather than single threats in isolation.

Building global change resilience: Concrete has the potential to ameliorate the negative effects of climate-driven ocean change on a newly-settled calcifying invertebrate.

Global climate change is driving sea level rise and increasingly frequent storm events, which are negatively impacting rapidly-growing coastal communities. To mitigate these impacts, coastal infrastructure must be further protected by upgrading hard defences. We propose that incorporating pH-buffering materials into these upgrades could safeguard marine organisms from the adverse effects of ocean acidification and ocean warming during the vulnerable transition from planktonic larvae to benthic juveniles. To test this, we examined the effects of ocean warming (24 or 27 °C), ocean acidification (pH 8.1, 7.9, 7.7), and substratum (concrete, greywacke, granite) in all combinations on the settlement success of an ecologically and commercially important sea urchin, Tripneustes gratilla. Low pH (7.9, 7.7) generally reduced the quantity and size of juveniles four weeks post-settlement, although this was partially ameliorated by increased temperature (24 vs. 27 °C). In the warmed and acidified treatments, settlement rates were lower on concrete than granite or greywacke, but two weeks post-settlement, juveniles on concrete were larger, and had longer spines and higher survival rates than on greywacke or granite, respectively. The benefits provided by concrete to newly-settled juveniles may be related to alkali chemicals leaching from concrete buffering low pH conditions in surrounding seawater and/or increased availability of bicarbonate in the boundary layers around its surface. Our results highlight the potential for pH-buffering materials to assist marine organisms in coping with the effects of changing ocean conditions, but further research is required to understand the generality and mechanism(s) driving the beneficial effects of concrete and to test pH-buffering materials in the field.