1.
Klin Lab Diagn
; 59(11): 50-3, 2014 Nov.
Artigo
em Russo
| MEDLINE
| ID: mdl-25850248
RESUMO
The article presents data concerning analysis of bioassays under suspicion of influenza A(H1N1)pdm09. The technique of back transcription polymerase chain reaction and sequencing was applied. The separation of native influenza virus A was implemented using passaging through developing chick embryos.
Assuntos
Hemaglutininas Virais/genética , Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/virologia , RNA Viral/isolamento & purificação , Animais , Embrião de Galinha , Hemaglutininas Virais/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H1N1/patogenicidade , Vírus da Influenza A Subtipo H1N1/ultraestrutura , Influenza Humana/genética , Influenza Humana/patologia , Microscopia Eletrônica , RNA Viral/genética
2.
Klin Lab Diagn
; (3): 41-4, 2013 Mar.
Artigo
em Russo
| MEDLINE
| ID: mdl-23808026
RESUMO
The external and internal control samples are used in case of polymerase chain reaction application to ensure validity of results. The external control samples are used to establish operability of reagents included into diagnostic kit. The internal control samples make it possible to check not only all the stages of reaction but also the process of amplification in each individual test tube with reaction mixture.