RESUMO
A direct method using 99mtechnecium to label the synthetic antimicrobial oligopeptide ubiquicidine with a molecular mass of 1.7 kDa has been developed. The radiochemical purity of the resultant labeled compound was more than 98%. A combination of ubiquicidine and 99mtechnecium showed stability in blood plasma. The biological study indicated that the complex was renally excreted from blood flow rapidly (the half-life is 30 min). It accumulated in the experimental inflammatory focus of laboratory animals, peaking 1-2 hours after intravenous injection when the inflammatory focus/intact tissue ratio amounted up to 2.6-3.4, which could yield satisfactory abscess scintigrams. The findings make it possible to consider ubiquicidine as a promising compound for the design of radiopharmaceutical to image inflammatory foci.
Assuntos
Compostos Radiofarmacêuticos , Proteínas Ribossômicas/farmacocinética , Pertecnetato Tc 99m de Sódio , Infecções Estafilocócicas , Animais , Modelos Animais de Doenças , Injeções Intravenosas , Cintilografia , Compostos Radiofarmacêuticos/administração & dosagem , Compostos Radiofarmacêuticos/farmacocinética , Ratos , Ratos Wistar , Proteínas Ribossômicas/uso terapêutico , Pertecnetato Tc 99m de Sódio/administração & dosagem , Pertecnetato Tc 99m de Sódio/farmacocinética , Infecções Estafilocócicas/diagnóstico por imagem , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/metabolismo , Staphylococcus/patogenicidade , Resultado do TratamentoRESUMO
IgG diagnosticum for measuring the concentration of 131I-labeled IgG antibodies to enteric antigen beta 1MA by the latex agglutination inhibition (LAI) test has been prepared on the basis of polyacrolein latexes. A method for the titration of anti-IgG antibodies with the use of the above diagnosticum has been developed, based on the late, agglutination (LA) test. The optimum conditions for the microtitration variant of the LA and LAI tests have been defined. High sensitivity, specificity and simplicity of analysis with the use of latex IgG diagnosticum have been demonstrated. The newly developed methods have been successfully used in laboratory trials of a new diagnostic radiopharmaceuticals for the assay of 131I-labeled antibodies in this preparation and for the detection of side effects of immunization on the recipients.
Assuntos
Imunoglobulina G/análise , Testes de Fixação do Látex/métodos , Acroleína , Animais , Antígenos/imunologia , Cães , Estudos de Avaliação como Assunto , Humanos , Látex , Testes de Fixação do Látex/instrumentação , Mecônio/imunologia , Camundongos , Tamanho da Partícula , Polímeros , Coelhos , RatosRESUMO
It has been shown by direct radiometry that 99m Tc-pertechnetate is excreted from the rat testes according to the exponential dependence, the maximum accumulation being observed 10 minutes after the injection (0.5% per 1 g tissue of the injected activity). Autoradiography has revealed that most of the radionuclide is located in the vascular system of the testes, whilst the minor part in the seminiferous tubules, the maximum accumulation being reached one hour after injection. It is concluded that 99mTc-pertechnetate behaviour pattern in the testes is first of all determined by its content in the vascular system of the organ.