Architectural anatomy of the human tibialis anterior presents morphological asymmetries between superficial and deep unipennate regions.

The tibialis anterior muscle plays a critical role in human ambulation and contributes to maintaining the upright posture. However, little is known about its muscle architecture in males and females. One hundred and nine physically active males and females were recruited. Tibialis anterior muscle thickness, pennation angle, and fascicle length were measured at rest in both unipennate regions of both legs using real-time ultrasound imaging. A linear mixed model was used with muscle thickness, pennation angle, or fascicle length as the dependent variables. All models were carried out with and without total leg lean mass and shank length as covariates. Causal mediation analysis was computed to explore the effect of muscle thickness on the relationship between fascicle length and pennation angle. There were no significant differences between dominant and nondominant legs regarding muscle architecture. Muscle thickness and pennation angle were greater in the deep than the superficial unipennate region in males (1.9 mm and 1.1°, p < 0.001) and women (3.4 mm and 2.2°, p < 0.001). However, the fascicle length was similar in both regions for both sexes. The differences remained significant after accounting for differences in leg lean mass and shank length. In both regions, muscle thickness was 1-3 mm greater in males and superficial pennation angle 2° smaller in females (both, p < 0.001). After accounting for leg lean mass and shank length, sex differences remained for muscle thickness (1.6 mm, p < 0.05) and pennation angle (3.4°, p < 0.001) but only in the superficial region. In both regions, leg lean mass and shank-adjusted fascicle length were 1.4 mm longer in females than males (p < 0.05). The causal mediation analysis revealed that the estimation of fascicle length was positive, suggesting that a 10% increase in muscle thickness would augment the fascicle length, allowing a 0.38° pennation angle decrease. Moreover, the pennation angle increases in total by 0.54° due to the suppressive effect of the increase in fascicle length. The estimated mediation, direct, and total effects were all significantly different from zero (p < 0.001). Overall, our results indicate that the architectural anatomy of the tibialis anterior shows sexual dimorphism in humans. Tibialis anterior presents morphological asymmetries between superficial and deep unipennate regions in both sexes. Lastly, our causal mediation model identified a suppressive effect of fascicle length on the pennation angle, suggesting that increments in muscle thickness are not always aligned with increments in fascicle length or the pennation angle.

Acute Mechanical, Neuromuscular, and Metabolic Responses to Different Set Configurations in Resistance Training.

ABSTRACT: Piqueras-Sanchiz, F, Cornejo-Daza, PJ, Sánchez-Valdepeñas, J, Bachero-Mena, B, Sánchez-Moreno, M, Martín-Rodríguez, S, García-García, Ó, and Pareja-Blanco, F. Acute mechanical, neuromuscular, and metabolic responses to different set configurations in resistance training. J Strength Cond Res 36(11): 2983-2991, 2022-The aim of this study was to investigate the effect of set configuration on mechanical performance, neuromuscular activity, metabolic response, and muscle contractile properties. Sixteen strength-trained men performed 2 training sessions in the squat exercise consisting of (a) 3 sets of 8 repetitions with 5 minutes rest between sets (3 × 8) and (b) 6 sets of 4 repetitions with 2 minutes rest between sets (6 × 4). Training intensity (75% one repetition maximum), total volume (24 repetitions), total rest (10 minutes), and training density were equalized between protocols. A battery of tests was performed before and after each protocol: (a) tensiomyography (TMG), (b) blood lactate and ammonia concentration, (c) countermovement jump, and (d) maximal voluntary isometric contraction in the squat exercise. Force, velocity, and power output values, along with electromyography data, were recorded for every repetition throughout each protocol. The 6 × 4 protocol resulted in greater mechanical performance (i.e., force, velocity, and power) and lower neuromuscular markers of fatigue (i.e., lower root mean square and higher median frequency) during the exercise compared with 3 × 8, particularly for the last repetitions of each set. The 3 × 8 protocol induced greater lactate and ammonia concentrations, greater reductions in jump height, and greater impairments in TMG-derived velocity of deformation after exercise than 6 × 4. Therefore, implementing lower-repetition sets with shorter and more frequent interset rest intervals attenuates impairments in mechanical performance, especially in the final repetitions of each set. These effects may be mediated by lower neuromuscular alterations, reduced metabolic stress, and better maintained muscle contractile properties.

Angiotensin-Converting Enzyme 2 (SARS-CoV-2 receptor) expression in human skeletal muscle.

The study aimed to determine the levels of skeletal muscle angiotensin-converting enzyme 2 (ACE2, the SARS-CoV-2 receptor) protein expression in men and women and assess whether ACE2 expression in skeletal muscle is associated with cardiorespiratory fitness and adiposity. The level of ACE2 in vastus lateralis muscle biopsies collected in previous studies from 170 men (age: 19-65 years, weight: 56-137 kg, BMI: 23-44) and 69 women (age: 18-55 years, weight: 41-126 kg, BMI: 22-39) was analyzed in duplicate by western blot. VO2 max was determined by ergospirometry and body composition by DXA. ACE2 protein expression was 1.8-fold higher in women than men (p = 0.001, n = 239). This sex difference disappeared after accounting for the percentage of body fat (fat %), VO2 max per kg of legs lean mass (VO2 max-LLM) and age (p = 0.47). Multiple regression analysis showed that the fat % (ß = 0.47) is the main predictor of the variability in ACE2 protein expression in skeletal muscle, explaining 5.2% of the variance. VO2 max-LLM had also predictive value (ß = 0.09). There was a significant fat % by VO2 max-LLM interaction, such that for subjects with low fat %, VO2 max-LLM was positively associated with ACE2 expression while as fat % increased the slope of the positive association between VO2 max-LLM and ACE2 was reduced. In conclusion, women express higher amounts of ACE2 in their skeletal muscles than men. This sexual dimorphism is mainly explained by sex differences in fat % and cardiorespiratory fitness. The percentage of body fat is the main predictor of the variability in ACE2 protein expression in human skeletal muscle.

Regulation of Nrf2/Keap1 signalling in human skeletal muscle during exercise to exhaustion in normoxia, severe acute hypoxia and post-exercise ischaemia: Influence of metabolite accumulation and oxygenation.

The Nrf2 transcription factor is induced by reactive oxygen and nitrogen species and is necessary for the adaptive response to exercise in mice. It remains unknown whether Nrf2 signalling is activated by exercise in human skeletal muscle. Here we show that Nrf2 signalling is activated by exercise to exhaustion with similar responses in normoxia (PIO2: 143 mmHg) and severe acute hypoxia (PIO2: 73 mmHg). CaMKII and AMPKα phosphorylation were similarly induced in both conditions. Enhanced Nrf2 signalling was achieved by raising Nrf2 total protein and Ser40 Nrf2 phosphorylation, accompanied by a reduction of Keap1. Keap1 protein degradation is facilitated by the phosphorylation of p62/SQSTM1 at Ser349 by AMPK, which targets Keap1 for autophagic degradation. Consequently, the Nrf2-to-Keap1 ratio was markedly elevated and closely associated with a 2-3-fold increase in Catalase protein. No relationship was observed between Nrf2 signalling and SOD1 and SOD2 protein levels. Application of ischaemia immediately at the end of exercise maintained these changes, which were reverted within 1 min of recovery with free circulation. While SOD2 did not change significantly during either exercise or ischaemia, SOD1 protein expression was marginally downregulated and upregulated during exercise in normoxia and hypoxia, respectively. We conclude that Nrf2/Keap1/Catalase pathway is rapidly regulated during exercise and recovery in human skeletal muscle. Catalase emerges as an essential antioxidant enzyme acutely upregulated during exercise and ischaemia. Post-exercise ischaemia maintains Nrf2 signalling at the level reached at exhaustion and can be used to avoid early post-exercise recovery, which is O2-dependent.

Mechanomyographic Measures of Muscle Contractile Properties are Influenced by Electrode Size and Stimulation Pulse Duration.

The aim was to determine the effects of changing pulse duration and electrode size on muscle contractile properties. Thirty-six healthy young male participated in the study (age 24.8 ± 5.8 years; height 178.2 ± 0.6 cm; body mass 71.8 ± 7.3 kg; self-reported weekly moderate intensity activity 3.5 ± 1.2 h·week-1). Tensiomyography was used to assess rectus femoris (RF) and vastus medialis (VM) muscles neuromuscular properties of the dominant leg according to the electrode size (3.2-5 cm) and the stimulus length (0.2, 0.5, and 1 ms). Maximal radial displacement (Dm); Contraction time (Tc); Delay time (Td); Sustained time (Ts) and Half relaxation time (Tr) were measured. Relative and absolute reliability was quantified. To analyze the effects of the electrode and the stimulus length, a repeated-measures analysis of variance was used. Dm and Tc parameters showed for both muscles an excellent relative (0.95-0.99) and absolute reliability (1.6-4.2%). However, Ts and Tr showed low values of absolute reliability (4.4-40.9%). The duration of the stimulus length applied to the RF and VM and electrode size significantly influences muscle's contractile properties (p < 0.05; η2p = 0.09-0.60). The Dm increases substantially as the duration of the stimulus increases and with the use of the larger electrode in both muscles. However, Tc and Td are less affected by both conditions and not entirely clear. Practically, our study suggests that a stimulus pulse duration of 1 ms together with a 5 × 5 cm electrode is necessary to reach a reliable and reproducible assessment of both RF and VM muscles contractile properties.

An integrative approach to the regulation of mitochondrial respiration during exercise: Focus on high-intensity exercise.

During exercise, muscle ATP demand increases with intensity, and at the highest power output, ATP consumption may increase more than 100-fold above the resting level. The rate of mitochondrial ATP production during exercise depends on the availability of O2, carbon substrates, reducing equivalents, ADP, Pi, free creatine, and Ca2+. It may also be modulated by acidosis, nitric oxide and reactive oxygen and nitrogen species (RONS). During fatiguing and repeated sprint exercise, RONS production may cause oxidative stress and damage to cellular structures and may reduce mitochondrial efficiency. Human studies indicate that the relatively low mitochondrial respiratory rates observed during sprint exercise are not due to lack of O2, or insufficient provision of Ca2+, reduced equivalents or carbon substrates, being a suboptimal stimulation by ADP the most plausible explanation. Recent in vitro studies with isolated skeletal muscle mitochondria, studied in conditions mimicking different exercise intensities, indicate that ROS production during aerobic exercise amounts to 1-2 orders of magnitude lower than previously thought. In this review, we will focus on the mechanisms regulating mitochondrial respiration, particularly during high-intensity exercise. We will analyze the factors that limit mitochondrial respiration and those that determine mitochondrial efficiency during exercise. Lastly, the differences in mitochondrial respiration between men and women will be addressed.

Supplementation with a Mango Leaf Extract (Zynamite®) in Combination with Quercetin Attenuates Muscle Damage and Pain and Accelerates Recovery after Strenuous Damaging Exercise.

Prolonged or unusual exercise may cause exercise-induced muscle damage (EIMD). To test whether Zynamite®, a mango leaf extract rich in the natural polyphenol mangiferin, administered in combination with quercetin facilitates recovery after EIMD, 24 women and 33 men were randomly assigned to two treatment groups matched by sex and 5 km running performance, and ran a 10 km race followed by 100 drop jumps to elicit EIMD. One hour before the competition, and every 8 hours thereafter for 24 hours, they ingested placebo (728 mg of maltodextrin) or 140 mg of Zynamite® combined with 140 mg of quercetin (double-blind). Although competition times were similar, polyphenol supplementation attenuated the muscle pain felt after the competition (6.8 ± 1.5 and 5.7 ± 2.2 a.u., p = 0.035) and the loss of jumping performance (9.4 ± 11.5 and 3.9 ± 5.2%, p = 0.036; p = 0.034) and mechanical impulse (p = 0.038) 24 hours later. The polyphenols attenuated the increase of serum myoglobin and alanine aminotransferase in men, but not in women (interaction p < 0.05). In conclusion, a single dose of 140 mg Zynamite® combined with 140 mg of quercetin, administered one hour before competition, followed by three additional doses every eight hours, attenuates muscle pain and damage, and accelerates the recovery of muscle performance.

Biochemical and Muscle Mechanical Postmarathon Changes in Hot and Humid Conditions.

Gutiérrez-Vargas, R, Martín-Rodríguez, S, Sánchez-Ureña, B, Rodríguez-Montero, A, Salas-Cabrera, J, Gutiérrez-Vargas, JC, Simunic, B, and Rojas-Valverde, D. Biochemical and muscle mechanical postmarathon changes in hot and humid conditions. J Strength Cond Res 34(3): 847-856, 2020-The aim of this study was to compare biochemical changes and mechanical changes in the lower-limb muscles before and after a marathon race in hot and humid conditions. Eighteen healthy runners participated in a marathon at between 28 and 34° C and 81% humidity in Costa Rica. Serum magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase, and hematocrit (HCT) were measured before and after the marathon. Tensiomyography measurements from the rectus femoris (RF) and vastus medialis, muscle displacement (Dm), contraction time (Tc), and velocities of contraction to 10 and 90% of Dm (V10 and V90) were obtained before and after the marathon. Postrace measurements showed a 544% increase in CPK (t(17): -6.925, p < 0.01), a 16% increase in HCT (t(17): -7.466, p < 0.01), a 29% decrease in Mg (t(17): 3.91, p = 0.001), a 2% decrease in body mass (t(17): 4.162, p = 0.001), a 4% increase in Tc of the RF (t(17): -2.588, p = 0.019), and a 12% increase in Dm of the RF (t(17): -2.131, p < 0.048) compared with prerace measurements. No significant biochemical or mechanical differences were found between runners in terms of their finish times. These findings showed that completing a marathon in hot and humid conditions induced a significant reduction in lower-limb muscle stiffness, body mass, and Mg, and increased neuromuscular fatigue, CPK, and HCT, because of muscle damage and dehydration. Knowledge of the effects of heat and humidity may be of value for coaches and sports medicine practitioners in developing effective hydration and recovery protocols for marathon runners in these special conditions.

Is Tensiomyography-Derived Velocity of Contraction a Sensitive Marker to Detect Acute Performance Changes in Elite Team-Sport Athletes?

PURPOSE: To examine the variations in the velocity of contraction (Vc) assessed using tensiomyography, vertical jumping ability, and sprinting speed induced by 4 different exercise protocols (ie, strength, sprint, plyometric, and technical training sessions) in 14 male national-team rugby players (age 21.8 [2.6] y, weight 83.6 [8.5] kg, and height 177.4 [6.7] cm). METHODS: Physical tests were conducted immediately before and after 4 distinct workouts in the following order: tensiomyography in the rectus femoris and biceps femoris muscles, squat and countermovement jumps, and 30-m sprint velocity. To analyze the differences in the assessed variables before and after each training session, the differences based on magnitudes were calculated. RESULTS: After strength and plyometric workouts, the players presented possible to almost certain impairments in sprint and jump performance and in the Vc of the rectus femoris (effect sizes 0.26-0.64). After the sprint-training session, possible to very likely decreases were observed in the squat jump, 30-m sprint, and Vc of the biceps femoris (effect sizes 0.21-0.44). By contrast, after the technical training, athletes demonstrated a possible increase in the squat jump and Vc in both muscles examined (effect sizes 0.13-0.20). CONCLUSIONS: The main finding of this research is that, for the vast majority of results, the direction of changes observed in Vc were the same as those observed in performance assessments. This suggests that Vc might be used as a sensitive marker of acute variations in speed and power performance of elite team-sport athletes.

Electromyographic Activity Evolution of Local Twitch Responses During Dry Needling of Latent Trigger Points in the Gastrocnemius Muscle: A Cross-Sectional Study.

OBJECTIVE: Trigger points (TrPs) are hypersensitive spots within taut bands of skeletal muscles that elicit referred pain and motor changes. Among the variety of techniques used for treating TrPs, dry needling is one of the most commonly applied interventions. The question of eliciting local twitch responses (LTRs) during TrP dry needling is unclear. Our main aim was to investigate the evolution of the electromyographic (EMG) peak activity of each LTR elicited during dry needling into latent TrPs of the gastrocnemius medialis muscle. METHODS: Twenty asymptomatic subjects with latent TrPs in the gastrocnemius medialis muscle participated in this cross-sectional study. Changes in EMG signal amplitude (root mean square [RMS]) with superficial EMG were assessed five minutes before, during, and five minutes after dry needling. The peak RMS score of each LTR was calculated (every 0.5 sec). RESULTS: Analysis of variance revealed a significant effect (F = 29.069, P <0.001) showing a significant decrease of RMS peak amplitude after each subsequent LTR. Differences were significant (P <0.001) during the first three LTRs, and stable until the end of the procedure. No changes (P =0.958) were found for mean RMS data at rest before (mean = 65.2 mv, 95% confidence interval [CI] = 47.3-83.1) and after (61.0 mv, 95% CI = 42.3-79.7) dry needling. CONCLUSIONS: We found that, in a series of LTRs elicited during the application of dry needling over latent TrPs in the medial gastrocnemius muscle, the RMS peak amplitude of each subsequent LTR decreased as compared with the initial RMS peak amplitude of previous LTRs. No changes in superficial EMG activity at rest were observed after dry needling of latent TrPs of the gastrocnemius medialis muscle.

Correction: Effects of moderate vs. high iso-inertial loads on power, velocity, work and hamstring contractile function after flywheel resistance exercise.

[This corrects the article DOI: 10.1371/journal.pone.0211700.].

Localization of Muscle Edema and Changes on Muscle Contractility After Dry Needling of Latent Trigger Points in the Gastrocnemius Muscle.

BACKGROUND: Myofascial trigger points (TrPs) are hyperirritable spots within taut bands of skeletal muscles that elicit local and referred pain when stimulated. Among the variety of techniques used for treating TrPs, dry needling (DN) is the most commonly applied intervention. The physiological mechanisms underlying the effects of DN remain to be elucidated. OBJECTIVE: To examine changes in skeletal muscle after DN in the area where the TrP is located. METHODS: We measured in vivo changes that occur in human skeletal muscle one hour after DN over a TrP with magnetic resonance imaging (MRI) and tensiomyography. The study included 18 asymptomatic subjects with a latent TrP in one medial gastrocnemius muscle, and the contralateral leg was used as control. RESULTS: The results showed that MRI signal intensity significantly increased one hour after the DN intervention, suggesting the presence of intramuscular edema. Tensiomyographic parameters showed higher muscle stiffness with an improvement in contraction time after DN. CONCLUSIONS: This is the first study showing intramuscular edema after TrP DN in human skeletal muscle. Future research should focus on using DN therapy in patients with active TrPs and on monitoring changes occurring at longer follow-up with imaging techniques.

Effects of moderate vs. high iso-inertial loads on power, velocity, work and hamstring contractile function after flywheel resistance exercise.

Flywheel iso-inertial training has been shown to positively affect muscular strength and sports performance (e.g. agility). However, implementing such eccentrically-biased training during a microcycle needs to be carefully planned due to its purported effects on the neuromuscular system that can last for hours/days post-exercise. This study aimed at using tensiomyography to verify the effects of different inertias during the hip extension exercise on the contractile function of biceps femoris and semitendinosus muscles of the dominant leg for up to 72 hours post-exercise. Thirty participants (24.4 ± 3.4 years) were divided into 0.075 or 0.1 kg·m2 inertia groups and a control group. Magnitude-based analysis was used for the comparisons. Several tensiomyography parameters were changed after both intensities of flywheel exercise (in most cases indicating a decrement in muscle stiffness), whereas most between-group differences suggested that in the semitendinosus muscle, the higher inertia (0.1 kg·m2) influenced the muscle stiffness parameters more (e.g. Dm = maximal radial displacement) while in the biceps femoris, the greater effect was caused by the lower inertia (0.075 kg·m2) (e.g. Tc = contraction time). Most changes in contractile properties of the investigated muscles occur within 24 hours post-exercise, but can persist for up to 72 hours. However, higher inertia (0.1 kg·m2) influenced the stiffness of the semitendinosus muscle more, while in the biceps femoris, the greater effect was caused by the lower inertia (0.075 kg·m2). These findings should be considered by practitioners when prescribing flywheel iso-inertial training.

Reliability and Measurement Error of Tensiomyography to Assess Mechanical Muscle Function: A Systematic Review.

Martín-Rodríguez, S, Loturco, I, Hunter, AM, Rodríguez-Ruiz, D, and Munguia-Izquierdo, D. Reliability and measurement error of tensiomyography to assess mechanical muscle function: A systematic review. J Strength Cond Res 31(12): 3524-3536, 2017-Interest in studying mechanical skeletal muscle function through tensiomyography (TMG) has increased in recent years. This systematic review aimed to (a) report the reliability and measurement error of all TMG parameters (i.e., maximum radial displacement of the muscle belly [Dm], contraction time [Tc], delay time [Td], half-relaxation time [½ Tr], and sustained contraction time [Ts]) and (b) to provide critical reflection on how to perform accurate and appropriate measurements for informing clinicians, exercise professionals, and researchers. A comprehensive literature search was performed of the Pubmed, Scopus, Science Direct, and Cochrane databases up to July 2017. Eight studies were included in this systematic review. Meta-analysis could not be performed because of the low quality of the evidence of some studies evaluated. Overall, the review of the 9 studies involving 158 participants revealed high relative reliability (intraclass correlation coefficient [ICC]) for Dm (0.91-0.99); moderate-to-high ICC for Ts (0.80-0.96), Tc (0.70-0.98), and ½ Tr (0.77-0.93); and low-to-high ICC for Td (0.60-0.98), independently of the evaluated muscles. In addition, absolute reliability (coefficient of variation [CV]) was low for all TMG parameters except for ½ Tr (CV = >20%), whereas measurement error indexes were high for this parameter. In conclusion, this study indicates that 3 of the TMG parameters (Dm, Td, and Tc) are highly reliable, whereas ½ Tr demonstrate insufficient reliability, and thus should not be used in future studies.