RESUMO
AIM: Study the microbial landscape and determine the interaction between biocenoses of skin, oropharynx and intestine mucous membranes during atopic dermatitis in children. MATERIALS AND METHODS: 60 children with atopic dermatitis were examined, bacteriologic study of skin, oropharynx, intestine was carried out. RESULTS: Significant changes were detected in both quantitative and qualitative composition of microbiocenosis of skin, oropharynx and intestine mucous membranes. Skin of patients is more frequently colonized by Staphylococcus aureus. Gram-positive bacteria dominated in oropharynx microflora. Comparative characteristics of microflora of skin and oropharynx mucous membrane revealed a direct of correlation. During microbiological study of intestine microflora, all the examined had microbial landscape disruptions of varying severity degree. CONCLUSION: Taking into consideration the direct correlation of microflora of skin and oropharynx mucous membrane during atopic dermatitis, seeding of oropharynx washes are recommended to be included into the examination complex of patients with subsequent correction of microbiocenosis. Examination of all the children with atopic dermatitis for the presence of intestine dysbiosis is advisable.
Assuntos
Dermatite Atópica/microbiologia , Disbiose/microbiologia , Microbiota , Mucosa/microbiologia , Técnicas de Tipagem Bacteriana , Criança , Pré-Escolar , Dermatite Atópica/patologia , Disbiose/patologia , Feminino , Humanos , Intestinos/microbiologia , Intestinos/patologia , Masculino , Mucosa/patologia , Orofaringe/microbiologia , Orofaringe/patologia , Pele/microbiologia , Pele/patologia , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus aureus/patogenicidade , Staphylococcus epidermidis/crescimento & desenvolvimento , Staphylococcus epidermidis/patogenicidadeAssuntos
Proteínas de Bactérias/genética , Consórcios Microbianos/fisiologia , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/genética , RNA Ribossômico 16S/genética , Bactérias Redutoras de Enxofre/genética , Proteínas de Bactérias/classificação , Proteínas de Bactérias/metabolismo , Temperatura Baixa , Estâncias para Tratamento de Saúde , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/classificação , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/metabolismo , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/classificação , Federação Russa , Análise de Sequência de DNA , Radioisótopos de Enxofre , Bactérias Redutoras de Enxofre/classificação , Bactérias Redutoras de Enxofre/isolamento & purificação , Bactérias Redutoras de Enxofre/metabolismoRESUMO
AIM: Long-term analysis of structure and resistance level ofpyogenic microflora in cardiac surgery hospital. MATERIALS AND METHODS: Data from microbiological studies carried out in Perm Regional Hospital No. 2 Institute of Heart from 2002 to 2010 was used. Isolation and identification of cultures was carried out by traditional methods, determination of antibiotic resistance--by disk diffusion method, sensitivity to disinfectants--by serial dilution method. RESULTS: Gram-positive bacteria predominate in the structure of clinical flora--enterococci and staphylococci (aureus--during the first years of observation, coagulase negative staphylococci--later). In 2010 an increase of relative rate of gram-negative bacteria, especially pseudomonas and acinetobacters, as well as Serratia marcescens was noted. Among all microorganism species a constant increase of antibiotic resistance level was noted. Clinical microflora is highly sensitive to the preparation for final disinfection, but at the same time is characterized by the generation of resistance to the current disinfectant. CONCLUSION: Monitoring of structure and properties of clinical microflora allows to introduce correction into antibacterial therapy schemes and epidemic control measures, as well as register the emergence of hospital strains. High level of drug resistance of the flora indicates the practicability of antibiotic rotation.
Assuntos
Infecções Bacterianas/microbiologia , Institutos de Cardiologia , Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Antibacterianos/farmacologia , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/etiologia , Desinfetantes/farmacologia , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Farmacorresistência Bacteriana Múltipla , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/genética , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/genética , Humanos , SupuraçãoRESUMO
We studied in vitro effects of mineral waters Ust-Kachkinskaya and Obukhovskaya on survival of gram-negative and gram-positive bacteria, candida fungi and on their sensitivity to antimicrobial drugs. The above mineral waters deteriorate viability of opportunistic bacterial and fungal flora and raise its sensitivity to antibiotics.