[Vitamin B12 is a diagnostic marker of decreased men reproductive function]. / Vitamin B12 kak diagnosticheskii marker snizheniia reproduktivnoi funktsii u muzhchin.

Although the relationship between the amount of vitamin B12 and the quality of sperm exists, but results are controversial and require several additional research. The objective of our study was to analyse the amount of vitamin B12 in the sperm samples from patients with chronic prostatitis and varicocele with accompanying asthenozoospermia, and to identify the relationship between the amount of vitamin B12 and asthenozoospermia. The research was carried out of men with chronic prostatitis and varicocele with accompanying asthenozoospermia and infertility at the age of 27±2 years. The material of the investigation was spermoplasm. A chemical microscopic examination of the ejaculate was carried out with a sperm analyzer and with the light microscopy. The amount of vitamin B12 in the spermoplasm was determined by the method of competitive ELISA. It was found that the level of vitamin B12 was 3.6 times lower in patients with chronic prostatitis III B and asthenozoospermia then in the control group. Among patients with varicocele of II and III grade and asthenozoospermia, the level of vitamin B12 was 4.4 times lower than in control group. A positive correlation relationship of average strength was revealed (r=0,683; p=0,001). Additionally, it was revealed that among patients with Chronic prostatitis III B and varicocele of II and III grades with accompanying asthenozoospermia, there was the positive correlation relationship of average strength (r=0,690; p=0,001) between the amount of vitamin B12 and sperm mobility. A decrease in vitamin B12 levels may serve as a marker of reproductive dysfunction in men.

[Hormonal disturbances and the treatment of refractory forms of congestive circulatory insufficiency]. / Gormonal'nye narusheniia i lechenie bol'nykh s refrakternymi formami zastoinoi zerdechnoi nedostatochnosti

In 109 patients with congestive circulatory insufficiency daily aldosterone excretion was examined by thin-layer chromatography, and in 50 patients aldosteronuria was compared with the excretion of electrolytes and haemodynamic parameters. The daily excretion of aldosterone under the effect of Prednisolone and other saluretics was studied along with the daily excretion of glucocorticoids under the effect of Prednisolone. The efficacy of a combination of drugs (Aldactone, Furosemid, Prednisolone) was studied in patients with refractory forms of circulatory insufficiency. The obtained data revealed an increase of the mineralocorticoid function and a decrease of the glucocorticoid function of the adrenals, a close correlation existing between the level of aldosteronuria, central haemodynamics pattern and electrolytes excretion. Therapy with Prednisolone results in a reduction of aldosteronuria, without causing any inhibition of the glucocorticoid function. The best therapeutic effect was obtained with a combination of Prednisolone, Aldactone, Furosemid, that caused distinct diuresis, natriuresis, increased blood plasma potassium levels, reduction of total dody fluid and body weight.