Serosurvey for equine piroplasms in horses and donkeys from North-Western Nigeria using IFAT and ELISA.

Equine piroplasmosis is caused by apicomplexan parasites, namely, Babesia caballi and Theileria equi, which are transmitted to equids principally through ticks. To ascertain the exposure of equines to agents of equine piroplasms, we tested serum samples collected from horses (n = 272) and donkeys (n = 170) in North-Western Nigeria for the presence of antibodies against B. caballi and T. equi using IFAT and ELISA. The seroprevalence of T. equi in the horses determined using IFAT and ELISA was 48.89% and 45.96%, respectively, while for B. caballi, it was 6.3% and 0.4%, respectively. For T. equi, the seroprevalence based on IFAT and ELISA results in donkeys was 14.1% and 2.9%, respectively, while for B. caballi, the seroprevalence was 2.4% and 0.6%, respectively, for ELISA and IFAT. Mixed infection detected in the horses using IFAT and ELISA was 5.5% and 0.4%, respectively, while no mixed infection was observed in the donkeys. The seroprevalence of T. equi was significantly (P < .0001) higher than that of B. caballi in both horses and donkeys. Comparatively, the IFAT detected a greater number of piroplasm seropositive animals than ELISA, indicating a difference in their diagnostic accuracy. Findings from this study confirm the existence of equine piroplasms in both horses and donkeys in North-Western Nigeria and highlights the need for robust and effective control measures against the disease.

Molecular evidence of Babesia caballi and Theileria equi in equines and ticks in Nigeria: prevalence and risk factors analysis.

Babesia caballi and Theileria equi are biological agents responsible for equine piroplasmosis (EP). We conducted a robust and extensive epidemiological study in Nigeria on the prevalence and risk factors of EP. Blood (468, both horses and donkeys) and ticks (201 pools) were screened using polymerase chain reaction (PCR). DNA of equine piroplasms was observed in tick pools with B. caballi amplified in Rhipicephalus evertsi evertsi only [minimum infection rate (MIR) of 7.6%] while T. equi was observed in R. e. evertsi (MIR, 61.6%), Hyalomma dromedarii (MIR, 23.7%) and H. truncatum (MIR, 50.0%). Overall results showed that 196/468 (41.9%) animals were positive for equine piroplasms (both B. caballi and T. equi). The prevalence for T. equi was 189/468 (40.4%) compared to 7/468 (1.5%) for B. caballi. In the horses and donkeys, respectively, the prevalence for T. equi was (39.9%; 112/281) and (41.2%; 77/187) compared with (1.4%; 4/281) and (1.6%; 3/187) due to B. caballi. Our analysis showed that location (Jigawa state), Talon breed, horses used for work and reproduction, unsatisfactory husbandry practices, contact with other mammals are risk factors that associated positivity to T. equi infection in horses, whilst horses kept on intensive management appeared to be less prone to infection. On the other hand, Jangora breed of donkeys and location (Jigawa state) are risk factors to infection with T. equi in donkeys. Findings suggest the persistence of EP in equids and ticks in Nigeria.

Parasitic and zoonotic meningoencephalitis in humans and equids: Current knowledge and the role of Halicephalobus gingivalis.

Halicephalobus gingivalis is a saprophytic nematode parasite that causes a rare form of fatal meningoencephalomyelitis in equids, humans, and ruminants. This nematode has neurotropic activity, but has also been found in the kidney, liver, lungs, optic nerves and even heart of its host. Despite the zoonotic potential and severity of the disease, the epidemiology, pathogenesis, life cycle, and risk factors are poorly understood. Cases have been reported from several countries in Europe countries and North America but none is recorded in Africa except Egypt. This review looks at the historical overview, morphology, diagnosis, treatment and summary of reported cases in humans and equids. We recommend the parasitic helminthic infection in the differential list of meningoencephalitis involving humans and animals worldwide despite its rareness.

Toxicity and anti-trypanosomal effects of ethanolic extract of Butyrospermum paradoxum (Sapotaceae) stem bark in rats infected with Trypanosoma brucei and Trypanosoma congolense.

The ethanolic extract of Butyrospermum paradoxum stem bark, commonly used in the traditional treatment of various diseases including animal and human trypanosomosis in north-eastern Nigeria, was tested for toxicity and anti-trypanosomal efficacy in rats infected with Trypanosoma congolense and Trypanosoma brucei. Following intra-peritoneal administration, the extract induced behavioural changes, morbidity and mortality in the rats. The symptoms observed included anorexia, dehydration, depression, prostration, coma and death. These symptoms were noted at high doses (> 800 mg/kg) only. At necropsy, the pathological lesions were mainly congestion and oedema of the lungs, bronchi, bronchioles and the kidney, hepatomegally and focal necrosis of the liver cells. The severity of the symptoms and lesions were dose related. The intra-peritoneal LD50 of the extract was 820 mg/kg. The extract produced anti-trypanosomal effect through the complete suppression or delay in parasite establishment with reduction in the level of parasitaemia and the severity of the attendant disease as well as enhanced survival of the rats infected with Trypanosoma congolense and Trypanosoma brucei. The results suggest that the folkloric medicinal application of the extracts of Butyrospermum paradoxum has a pharmacological basis.