The impact of skin preparation method on electrocardiogram quality in horses.

Objective: Several skin preparation techniques are used in electrocardiogram (ECG) monitoring of horses. The objective of this study was to determine which methods produce the greatest signal quality using textile electrodes and standard silver/silver chloride (Ag/AgCl) electrodes. Animals and samples: Electrocardiogram data were collected using textile and Ag/AgCl electrodes simultaneously for 4 skin preparation techniques in 6 horses. Procedure: The effects of skin preparation (cleansing with isopropyl alcohol, with or without shaving the hair) and the effects of the application of a conductive gel were assessed using metrics of signal quality. Results: Shaving and cleansing with alcohol had no effect on signal quality for either electrode type. The Ag/AgCl electrodes contain a solid gel, and the application of additional gel did not affect signal quality. Data quality was significantly improved when gel was applied to textile electrodes. Furthermore, there was no difference in signal quality between electrode types when gel was used. Conclusion and clinical relevance: This study suggests that skin preparation by cleansing and/or shaving does not have a significant effect on equine ECG signal quality. When gel is used, textile electrodes are a practical alternative for Ag/AgCl electrodes, as they produce ECG recordings of the same quality.

Impact de la méthode de préparation de la peau sur la qualité de l'électrocardiogramme chez le cheval. Objectif: Plusieurs techniques de préparation de la peau sont utilisées lors de la surveillance électrocardiographique (ECG) des chevaux. L'objectif de cette étude était de déterminer quelles méthodes produisent la meilleure qualité de signal en utilisant des électrodes textiles et des électrodes standard argent/chlorure d'argent (Ag/AgCl). Animaux et échantillons: Les données d'électrocardiogramme ont été obtenues simultanément à l'aide d'électrodes textiles et d'électrodes Ag/AgCl pour 4 techniques de préparation cutanée chez 6 chevaux. Procédure: Les effets de la préparation de la peau (nettoyage à l'alcool isopropylique, avec ou sans rasage des cheveux) et les effets de l'application d'un gel conducteur ont été évalués à l'aide de métriques de qualité du signal. Résultats: Le rasage et le nettoyage à l'alcool n'ont eu aucun effet sur la qualité du signal pour les deux types d'électrodes. Les électrodes Ag/AgCl contiennent un gel solide et l'application de gel supplémentaire n'a pas affecté la qualité du signal. La qualité des données a été considérablement améliorée lorsque le gel a été appliqué sur des électrodes textiles. De plus, il n'y avait aucune différence dans la qualité du signal entre les types d'électrodes lorsque du gel était utilisé. Conclusion et pertinence clinique: Cette étude suggère que la préparation de la peau par nettoyage et/ou rasage n'a pas d'effet significatif sur la qualité du signal ECG équin. Lorsque du gel est utilisé, les électrodes textiles constituent une alternative pratique aux électrodes Ag/AgCl, car elles produisent des enregistrements ECG de même qualité.(Traduit par Dr Serge Messier).

Validation of an Equine Smart Textile System for Heart Rate Variability: A Preliminary Study.

Electrocardiograms (ECGs), and associated heart rate (HR) and heart rate variability (HRV) measurements, are essential in assessing equine cardiovascular health and fitness. Smart textiles have gained popularity, but limited validation work has been conducted. Therefore, the objective of this study was to compare HR and HRV data obtained using a smart textile system (Myant) to the gold-standard telemetric device (Televet). Simultaneous ECGs were obtained using both systems in seven horses at rest and during a submaximal exercise test. Bland-Altman tests were used to assess agreement between the two devices. Strong to perfect correlations without significant differences between the two devices were observed for all metrics assessed. During exercise, mean biases of 0.31 bpm (95% limits of agreement: -1.99 to 2.61) for HR, 1.43 ms (-11.48 to 14.33) for standard deviation of R-R intervals (SDRR), and 0.04 (-2.30 to 2.38) for the HRV triangular index (TI) were observed. A very strong correlation was found between the two devices for HR (r = 0.9993, p < 0.0001) and for HRV parameters (SDRR r = 0.8765, p < 0.0001; TI r = 0.8712, p < 0.0001). This study demonstrates that a smart textile system is reliable for assessment of HR and HRV of horses at rest and during submaximal exercise.

Cartilage-Sparing Properties of Equine Omega Complete in an Organ Culture Model of Cartilage Inflammation.

The purpose of this study was to determine anti-inflammatory and/or chondroprotective effects of Equine Omega Complete (EOC) on cartilage explants stimulated with lipopolysaccharide (LPS). Explants were aseptically prepared from the intercarpal joints of 17 market-weight pigs and placed in culture at 37°C for a total of 120 hours. For the final 96 hours, explants were conditioned with a simulated digestion extract of EOC (0, 36 or 180 µL/mL), and for the final 48 hours explants were stimulated with LPS (0 or 15µg/mL). Media was removed and replaced every 24 hours. Samples from the final 48 hours were analyzed for biomarkers of cartilage inflammation (prostaglandin E2 [PGE2] and nitric oxide [NO]) and cartilage structure (glycosaminoglycan [GAG]). At the end of the culture period cartilage explants were stained for an estimate of cell viability. Stimulation of unconditioned explants with LPS significantly increased media concentrations of PGE2, GAG and NO compared with that from unstimulated explants. LPS stimulation did not significantly affect cell viability. Both concentrations of EOC prevented significant LPS-stimulated cartilage release of GAG without impairing chondrocyte viability. No other effects of treatment were observed. These data provide evidence for a non-cytotoxic, chondroprotective effect of EOC in cartilage. This in vitro experiment supports the use of EOC in protecting against the detrimental effects of inflammation on cartilage structure.

Frequency of cardiac arrhythmias in horses during straight and untethered swimming.

BACKGROUND: Cardiac arrhythmias have not been previously reported in horses while swimming. OBJECTIVES: To describe the type and frequency of encountered arrhythmias during repetitive swimming cycles. STUDY DESIGN: Descriptive observational study. METHODS: Sixteen horses swam five pool lengths (75 m), each separated by an active recovery walk. Continuous electrocardiograms (ECGs) were recorded (n = 80) and analysed during the pre-swim, swim and active-recovery periods. Arrhythmias were categorised as sinus arrhythmia (SA), sinus block, sinus pause (compensatory and non-compensatory), second degree atrioventricular block (2AVB) for physiological arrhythmias, supraventricular premature depolarisation (SVPD) and ventricular premature depolarisation (VPD) for non-physiological arrhythmias. A linear mixed-effects model was used to examine the effects of repetitive swim lengths on arrhythmias and swimming parameters. Data were reported as median [interquartile range]. RESULTS: Fifteen horses (94%) experienced at least one arrhythmia; however, the frequency remained low and 2AVB were only observed during the pre-swim period. The swimming heart rate (HR) was 162 bpm [141;173]. Sinus blocks, sinus pauses, SA, SVPD and VPD were all recorded at least once during swimming. Except for one VPD couplet, all premature depolarisations were isolated. During active-recovery, the HR was 105 bpm [103;106], with SA observed in 13 horses (81%), isolated SVPD in six horses (38%), sinus pause in one horse (6%) but no VPD present. MAIN LIMITATIONS: Limited number of horses precluding population prevalence assessment. CONCLUSION AND CLINICAL IMPORTANCE: High-quality underwater ECGs were acquired in swimming horses for the first time. The frequency of arrhythmias remained low and rare pathological arrhythmias were observed during repetitive swimming and active-recovery cycles. Swimming with active-recovery periods is not a high-risk cardio-arrhythmic exercise.

HISTORIAL: Arritmias cardiacas no han sido previamente descritas en caballos nadando. OBJETIVOS: Describir el tipo y frecuencia de arritmias encontradas durante ciclos de natación repetitivos. DISEÑO DEL ESTUDIO: Estudio descriptivo observacional. MÉTODOS: Diez y seis caballos nadaron cinco largos de piscina (75 m), cada uno separado por una caminata de recuperación. Electrocardiogramas continuos (ECGs) fueron grabados (n = 80) y analizados durante el pre-natación, natación y periodos de recuperación activa. Las arritmias fueron categorizadas en arritmia sinusal (SA), bloqueo sinusal, pausa sinusal (compensatoria y no compensatoria), bloqueo atrio ventricular de segundo grado (2AVB) para las arritmias fisiológicas, y en despolarización supraventricular prematura (SVPD) y despolarización ventricular prematura (VPD) para las arritmias no fisiológicas. Se utilizo un modelo linear de efecto mixto para examinar los efectos de nadar largos de piscina en forma repetitiva sobre las arritmias y parámetros de natación. Los datos fueron reportados como mediana [rango intercuartil]. RESULTADOS: Quince caballos (94%) sufrieron de al menos una arritmia, sin embargo la frecuencia permaneció baja. 2AVB fueron observados solo durante el periodo de pre-natación. La frecuencia cardiaca durante la natación (HR) fue de 162 bpm [141;173]. Bloqueos sinusales, pausas sinusales, SA, SVPD y VPD fueron todos registrados por lo menos una vez durante la natación. Excepto por un par de VPD, todas las despolarizaciones fueron aisladas. Durante la recuperación activa, la HR fue de 105 bpm [103;106], con SA observado en 13 caballos (81%), SVPD aislados en 6 caballos (38%), pausa sinusal en 1 caballos (6%) pero ningún VPD presente. LIMITACIONES PRINCIPALES: Número limitado de caballos lo que imposibilita hacer un asesoramiento de la prevalencia en la población. CONCLUSIÓN E IMPORTANCIA CLÍNICA: Por primera vez, se adquirieron ECGs debajo del agua de alta calidad en caballos nadando. La frecuencia de las arritmias permaneció baja y se observaron arritmias patológicas raramente durante la natación repetitiva y los ciclos de recuperación activa. Nadar con periodos de recuperación activa no es un ejercicio de alto riesgo cardio-arrítmico.

Free Swimming and Exercise-Induced Pulmonary Hemorrhage in Endurance Horses: A Preliminary Study.

Swimming is used for rehabilitation of musculoskeletal injuries and for conditioning to improve equine fitness. However, there are anecdotal reports that suggest that tethered swimming can induce epistaxis, likely secondary to exercise-induced pulmonary hemorrhage (EIPH). The objective of this observational, prospective study was to investigate if EIPH occurs during intensive free-swimming training sessions (5 × 70m) using 15 endurance horses. On tracheo-bronchoscopic evaluations following swimming, low grade mucus scores were observed, but no tracheal blood was observed. Bronchoalveolar lavage fluid analysis revealed a low cellularity, and the median red blood cell count (RBCs) was 271 cells/µL (interquartile range 150-363 cells/µL), which is much lower than the threshold of RBCs >1,000 cells/µL for horses to be considered positive for EIPH. Therefore, free swimming does not seem to predispose endurance horses to EIPH following a typical free-swimming training session.

A Smart Textile Band Achieves High-Quality Electrocardiograms in Unrestrained Horses.

Electrocardiography (ECG) is an essential tool in assessing equine health and fitness. However, standard ECG devices are expensive and rely on the use of adhesive electrodes, which may become detached and are associated with reduced ECG quality over time. Smart textile electrodes composed of stainless-steel fibers have previously been shown to be a suitable alternative in horses at rest and during exercise. The objective of this study was to compare ECG quality using a smart textile girth band knit with silver and carbon yarns to standard adhesive silver/silver chloride (Ag/AgCl) electrodes. Simultaneous three-lead ECGs were recorded using a smart textile band and Ag/AgCl electrodes in 22 healthy, mixed-breed horses that were unrestrained in stalls. ECGs were compared using the following quality metrics: Kurtosis (k) value, Kurtosis signal quality index (kSQI), percentage of motion artifacts (%MA), peak signal amplitude, and heart rate (HR). Two-way ANOVA with Tukey's multiple comparison tests was conducted to compare each metric. No significant differences were found in any of the assessed metrics between the smart textile band and Ag/AgCl electrodes, with the exception of peak amplitude. Kurtosis and kSQI values were excellent for both methods (textile mean k = 21.8 ± 6.1, median kSQI = 0.98 [0.92−1.0]; Ag/AgCl k = 21.2 ± 7.6, kSQI = 0.99 [0.97−1.0]) with <0.5% (<1 min) of the recording being corrupted by MAs for both. This study demonstrates that smart textiles are a practical and reliable alternative to the standard electrodes typically used in ECG monitoring of horses.

Kinematic Analysis During Straight Line Free Swimming in Horses: Part 1 - Forelimbs.

Background: Swimming is used for rehabilitation and conditioning purposes in equine sports medicine despite the lack of understanding of equine swimming kinematics. The aim of this study was to assess forelimb joints kinematics (elbow, carpus, and fetlock) in swimming horses. The specific objectives were 1- to calculate and compare joint angles in swimming vs. passive mobilizations (PM), 2- to determine joint angular velocities during a swimming stride cycle. Methods: Eleven elite endurance horses swam in a 100-m straight pool. Underwater (swimming) and overground (PM) videos were recorded from the horses' left side. Joint markers were applied on the lateral hoof wall, lateral metacarpal epicondyle, ulnar carpal bone, lateral humeral epicondyle, and the greater tubercle of humerus, from which elbow, carpus and fetlock angles, and angular velocities were obtained. As a reference, maximal fetlock, carpus, and elbow flexion/extension angles were determined during PM overground. Differences between angle extrema, angular velocities and range of motion (ROM) were compared. Results: Carpus and fetlock ROM were significantly smaller (p < 0.001) during swimming when compared with PM, while there was no difference in elbow ROM between both situations. The carpus had the greatest ROM of all joints during swimming. Absolute angular velocities values of all joints during swimming were greater during retraction than protraction (p < 0.001). When compared to other joints during protraction, the carpus joint reached the highest angular velocity. Conclusion: Swimming, as a rehabilitation exercise, has the potential to benefit horses where great elbow ROM with a moderate carpus and fetlock extension are wanted.

Consensus for the General Use of Equine Water Treadmills for Healthy Horses.

Water treadmill exercise has become popular in recent years for the training and rehabilitation of equine athletes. In 2019, an equine hydrotherapy working group was formed to establish what was commonly considered to be best practice in the use of the modality. This article describes the process by which general guidelines for the application of water treadmill exercise in training and rehabilitation programmes were produced by the working group. The guidelines describe the consensus reached to date on (1) the potential benefits of water treadmill exercise, (2) general good practice in water treadmill exercise, (3) introduction of horses to the exercise, (4) factors influencing selection of belt speed, water depth and duration of exercise, and (5) monitoring movement on the water treadmill. The long-term goal is to reach a consensus on the optimal use of the modality within a training or rehabilitation programme. Collaboration between clinicians, researchers and experienced users is needed to develop research programmes and further guidelines regarding the most appropriate application of the modality for specific veterinary conditions.

Kinematic Analysis During Straight Line Free Swimming in Horses: Part 2 - Hindlimbs.

BACKGROUND: Swimming is used for rehabilitation and conditioning purposes in equine sports medicine. We described the swimming kinematics of the equine forelimbs in Part 1. The aim of Part 2 is to assess stifle, tarsus, and hind fetlock joints kinematics in swimming horses. The objectives were 1- to calculate and compare joint angles during swimming against passive mobilizations (PM), 2- to determine joints angular velocities during a swimming stride cycle. METHODS: Eleven elite endurance horses were used to swim in a 100-meter straight pool. Underwater (swimming) and overground PM videos were recorded from the horses' left side. Joint markers were applied on the lateral hoof wall, lateral metatarsal epicondyle, lateral aspect of the talus, lateral femoral epicondyle, and great trochanter of the femur. As a reference, maximal fetlock, tarsus, and stifle flexion/extension angles were determined during PM overground. Differences between angle extrema, angular velocities, and range of motion (ROM) were statistically compared. RESULTS: The tarsus ROM was similar during PM and swimming. The stifle and fetlock ROM were greater during PM, although the stifle flexion was greater during swimming. The stifle and tarsus had the greatest hindlimb angular velocity during the swimming cycle. Greater angular velocities were observed during the retraction phase for all the hindlimb joints. CONCLUSION: A short retraction phase with great angular velocity for the joints of interest characterized the swimming pattern observed. Swimming may be beneficial in horses when an increased ROM of the tarsus and stifle or a reduced fetlock extension is indicated for rehabilitation purposes.

Effects of exercise, furosemide, blood depletion, and reinfusion on body fluid compartment volumes in horses.

High pulmonary blood pressure contributes to exercise-induced pulmonary hemorrhage. The objective of this study was to use bioimpedance spectroscopy to assess body fluid compartment volumes under 3 conditions in 6 racehorses: i) Pre- and post-supramaximal treadmill exercise (control); ii) Exercise 4 hours after furosemide (0.5 mg/kg body weight, IV); iii) Exercise, removal of ~14 L of blood and subsequent reinfusion of the blood. Statistical analysis used linear mixed effects models. Body compartment volumes did not change during the control runs. Total body water (TBW) (P = 0.007, P = 0.007), extracellular fluid (ECF) (P = 0.003, P = 0.003), and intracellular fluid (ICF) volumes (P = 0.04, P = 0.04) decreased pre- and post-exercise following furosemide administration. The ICF trended to decrease (P = 0.07) after slow removal of blood. Blood reinfusion increased TBW (P = 0.02, P = 0.02) and ICF (P = 0.005, P = 0.005) pre- and post-exercise.

Effets de l'exercice, du furosémide, de la diminution sanguine et de la ré-infusion sur les volumes des liquides corporels compartimentés chez les chevaux. Une pression sanguine pulmonaire élevée contribue à des hémorragies pulmonaires induites par l'exercice. L'objectif de la présente étude était d'utiliser la spectroscopie à bio-impédance pour évaluer les volumes des liquides corporels compartimentés sous trois conditions chez six chevaux de course : i) Pré- et post-supramaximal exercice au tapis roulant (témoin); ii) Exercice 4 h après administration de furosémide (0,5 mg/kg de poids corporel, IV); iii) Exercice, retrait d'environ 14 L de sang et ré-infusion subséquente du sang. Les analyses statistiques utilisaient des modèles linéaires à effets mixtes. Les volumes des compartiments corporels n'ont pas changé durant les essais témoins. Les volumes de la quantité totale d'eau corporelle (TBW) (P = 0,007, P = 0,007), de liquide extracellulaire (ECF) (P = 0,003, P = 0,003) et liquide intracellulaire (ICF) (P = 0,04, P = 0,04) ont diminué pré- et post-exercice à la suite de l'administration de furosémide. L'ICF avait tendance à diminuer (P = 0,07) à la suite du lent retrait de sang. La ré-infusion de sang augmenta la TBW (P = 0,02, P = 0,02) et l'ICF (P = 0,005, P = 0,005) pré- et post-exercice.(Traduit par Dr Serge Messier).