Elevated Magnesium Concentrations Altered Freshwater Assemblage Structures in a Mesocosm Experiment.

Magnesium (Mg) is a mining-related contaminant in the Alligators Rivers Region of tropical northern Australia. A mesocosm experiment was used to assess Mg toxicity to aquatic freshwater assemblages. Twenty-five 2700-L tubs were arranged, stratified randomly, on the bed of Magela Creek, a seasonally flowing, sandy stream channel in the Alligator Rivers Region of northern Australia. The experiment comprised 5 replicates of 4 nominal Mg treatments, 2.5, 7.5, 23, and 68 mg L-1 , and a control. Phytoplankton biomass, and diatom, zooplankton, and macroinvertebrate assemblages present in the treatment tubs were sampled before and after Mg addition. A significant negative relationship between phytoplankton biomass and Mg was observed 4 wk after Mg addition as measured by chlorophyll a concentrations (r2 = 0.97, p = 0.01). This result was supported by reductions in some major phytoplankton groups in response to increasing Mg concentrations, in the same experiment and from independent field studies. There was a significant negative relationship between zooplankton assemblage similarity (to control) and Mg concentrations (r2 = 0.96, p = 0.002). Seven weeks after Mg addition, macroinvertebrate assemblages were dominated by 3 microcrustacean groups (Ostracoda, Cladocera, and Copepoda), each reaching maximum abundance at intermediate Mg concentrations (i.e., unimodal responses). The responses of phytoplankton and zooplankton were used to derive assemblage effect concentrations (Mg concentrations resulting in x% of the assemblage change [ECx]). Magnesium concentrations resulting in assemblage EC01 values were <3 mg L-1 . Together with candidate guideline values from other laboratory- and field-based lines of evidence, the mesocosm EC01 values were incorporated into a weight-of-evidence framework for a robust regulatory approach to environmental protection. Environ Toxicol Chem 2020;39:1973-1987. © 2020 Commonwealth of Australia. Published by Wiley Periodicals LLC on behalf of SETAC.

Engineered river flow-through to improve mine pit lake and river values.

Mine pit lakes may develop at mine closure when mining voids extend below groundwater levels and fill with water. Acid and metalliferous drainage (AMD) and salinity are common problems for pit lake water quality. Contaminated pit lake waters can directly present significant risk to both surrounding and regional communities and natural environmental values and limit beneficial end use opportunities. Pit lake waters can also discharge into surface and groundwater; or directly present risks to wildlife, stock and human end users. Riverine flow-through is increasingly proposed to mitigate or remediate pit lake water contamination using catchment scale processes. This paper presents the motivation and key processes and considerations for a flow-through pit lake closure strategy. International case studies as precedent and lessons for future application are described from pit lakes that use or propose flow-through as a key component of their mine closure design. Chemical and biological processes including dilution, absorption and flocculation and sedimentation can sustainably reduce pit lake contaminant concentrations to acceptable levels for risk and enable end use opportunities to be realised. Flow-through may be a valid mine closure strategy for pit lakes with poor water quality. However, maintenance of existing riverine system values must be foremost. We further suggest that decant river water quality may, in some circumstances, be improved; notably in examples of meso-eutrophic river waters flowing through slightly acidic pit lakes. Flow-through closure strategies must be scientifically justifiable and risk-based for both lake and receptors potentially affected by surface and groundwater transport. Due to the high-uncertainty associated with this complex strategy, biotic and physico-chemical attributes of both inflow and decant river reaches as well as lake should be well monitored. Monitoring should directly feed into an adaptive management framework discussed with key stakeholders with validation of flow-through as a sustainable strategy prior to mine relinquishment.

Assessment of factors limiting algal growth in acidic pit lakes--a case study from Western Australia, Australia.

Open-cut mining operations can form pit lakes on mine closure. These new water bodies typically have low nutrient concentrations and may have acidic and metal-contaminated waters from acid mine drainage (AMD) causing low algal biomass and algal biodiversity. A preliminary study was carried out on an acidic coal pit lake, Lake Kepwari, in Western Australia to determine which factors limited algal biomass. Water quality was monitored to obtain baseline data. pH ranged between 3.7 and 4.1, and solute concentrations were slightly elevated to levels of brackish water. Concentrations of N were highly relative to natural lakes, although concentrations of FRP (<0.01 mg/L) and C (total C 0.7-3.7 and DOC 0.7-3.5 mg/L) were very low, and as a result, algal growth was also extremely low. Microcosm experiment was conducted to test the hypothesis that nutrient enrichment will be able to stimulate algal growth regardless of water quality. Microcosms of Lake Kepwari water were amended with N, P and C nutrients with and without sediment. Nutrient amendments under microcosm conditions could not show any significant phytoplankton growth but was able to promote benthic algal growth. P amendments without sediment showed a statistically higher mean algal biomass concentration than controls or microcosms amended with phosphorus but with sediment did. Results indicated that algal biomass in acidic pit lake (Lake Kepwari) may be limited primarily by low nutrient concentrations (especially phosphorus) and not by low pH or elevated metal concentrations. Furthermore, sediment processes may also reduce the nutrient availability.

Bioremediation of Acidic and Metalliferous Drainage (AMD) through organic carbon amendment by municipal sewage and green waste.

Pit lakes (abandoned flooded mine pits) represent a potentially valuable water resource in hot arid regions. However, pit lake water is often characterised by low pH with high dissolved metal concentrations resulting from Acidic and Metalliferous Drainage (AMD). Addition of organic matter to pit lakes to enhance microbial sulphate reduction is a potential cost effective remediation strategy. However, cost and availability of suitable organic substrates are often limiting. Nevertheless, large quantities of sewage and green waste (organic garden waste) are often available at mine sites from nearby service towns. We treated AMD pit lake water (pH 2.4) from tropical, North Queensland, Australia, with primary-treated sewage sludge, green waste, and a mixture of sewage and green waste (1:1) in a controlled microcosm experiment (4.5 L). Treatments were assessed at two different rates of organic loading of 16:1 and 32:1 pit water:organic matter by mass. Combined green waste and sewage treatment was the optimal treatment with water pH increased to 5.5 in only 145 days with decreases of dissolved metal concentrations. Results indicated that green waste was a key component in the pH increase and concomitant heavy metal removal. Water quality remediation was primarily due to microbially-mediated sulphate reduction. The net result of this process was removal of sulphate and metal solutes to sediment mainly as monosulfides. During the treatment process NH(3) and H(2)S gases were produced, albeit at below concentrations of concern. Total coliforms were abundant in all green waste-treatments, however, faecal coliforms were absent from all treatments. This study demonstrates addition of low-grade organic materials has promise for bioremediation of acidic waters and warrants further experimental investigation into feasibility at higher scales of application such as pit lakes.

Aquatic toxicity of magnesium sulfate, and the influence of calcium, in very low ionic concentration water.

The toxicity of magnesium sulfate (MgSO(4)), and the influence of calcium (Ca), were assessed in very soft freshwater (natural Magela Creek water [NMCW]) using six freshwater species (Chlorella sp., Lemna aequinoctialis, Amerianna cumingi, Moinodaphnia macleayi, Hydra viridissima, and Mogurnda mogurnda). The study involved five stages: toxicity of MgSO(4) in NMCW, determination of the toxic ion, influence of Ca on Mg toxicity, toxicity of MgSO(4) at an Mg:Ca mass ratio of 9:1, and derivation of water quality guideline values for Mg. The toxicity of MgSO(4) was higher than previously reported, with chronic median inhibition concentration (IC50)/acute median lethal concentration (LC50) values ranging from 4 to 1,215 mg/L, as Mg. Experiments exposing the 3 most sensitive species (L. aequinoctialis, H. viridissima, and A. cumingi) to Na(2)SO(4) and MgCl(2) confirmed that Mg was the toxic ion. Additionally, Ca was shown to have an ameliorative effect on Mg toxicity. For L. aequinoctialis and H. viridissima, Mg toxicity at the IC50 concentration was eliminated at Mg:Ca (mass) ratios of < or =10:1 and < or =9:1, respectively. For A. cumingi, a 10 to 30% effect persisted at the IC50 concentration at Mg:Ca ratios <9:1. The toxicity of MgSO(4) in NMCW at a constant Mg:Ca ratio of 9:1 was lower than at background Ca, with chronic IC50/acute LC50 values from 96 to 4,054 mg/L, as Mg. Water quality guideline values for Mg (to protect 99% of species) at Mg:Ca mass ratios of >9:1 and < or =9:1 were 0.8 and 2.5 mg/L, respectively. Magnesium can be toxic at concentrations approaching natural background levels, but toxicity is dependent on Ca concentrations, with exposure in very low ionic concentration, Ca-deficient waters posing the greatest risk to aquatic life.

Vulnerability of organic acid tolerant wetland biota to the effects of inorganic acidification.

Inland waterbodies are often naturally acidic but are these ecosystems pre-adapted to inorganic acidification e.g., by acid sulfate soils (ASS)? We conducted a controlled mesocosm experiment with inorganically acidified wetland water and wetland sediment replicates to pH 3 from a naturally acidic (pH 3.9, conductivity=74microScm(-1)) wetland in south-western Australia. Following acidification, dissolved organic carbon and nitrogen declined, and chlorophyll a dropped to zero. Inorganic acidification mobilised metals from sediment sods with increased water concentrations of Cu, Fe, Mn, Ca, Mg and Al. Acidification showed no significant effect on diatom assemblage. Nonetheless, greatly reduced abundance and diversity of grazing zooplankton was observed. Macroinvertebrates generally showed abundance decreases, although filterer-collector taxa increased. Decreased primary production reduced functional diversity and consumer biomasses. These results suggest likely impact to ecosystem functioning of low pH, weakly-buffered and stained wetlands if exposed to inorganic acidification.

Toxicity of acid mine pit lake water remediated with limestone and phosphorus.

Pit lakes are increasingly common worldwide and have potential to provide many benefits. However, lake water toxicity may require remediation before beneficial end uses can be realised. Three treatments to remediate AMD (pH approximately 4.8) pit lake water containing elevated concentrations of Al and Zn from Collie, Western Australia were tested in mesocosms. Treatments were: (a) limestone neutralisation (L), (b) phosphorus amendment (P), and (c) combined limestone neutralisation and phosphorus amendment (L+P). Laboratory bioassays with Ceriodaphnia cf. dubia, Chlorella protothecoides and Tetrahymena thermophila assessed remediation. Limestone neutralisation increased pH and reduced heavy metal concentrations by 98% (Al) to 14% (Mg), removing toxicity to the three test species within 2 months. Phosphorus amendment removed toxicity after 6 months of treatment. However, phosphorus amendment to prior limestone neutralisation failed to reduce toxicity more than limestone neutralisation alone. Low concentrations of both phosphorus and nitrogen appear to limit phytoplankton population growth in all treatments.