Mitochondrial dysfunction underlies impaired neurovascular coupling following traumatic brain injury.

Traumatic brain injury (TBI) involves an acute injury (primary damage), which may evolve in the hours to days after impact (secondary damage). Seizures and cortical spreading depolarization (CSD) are metabolically demanding processes that may worsen secondary brain injury. Metabolic stress has been associated with mitochondrial dysfunction, including impaired calcium homeostasis, reduced ATP production, and elevated ROS production. However, the association between mitochondrial impairment and vascular function after TBI is poorly understood. Here, we explored this association using a rodent closed head injury model. CSD is associated with neurobehavioral decline after TBI. Craniotomy was performed to elicit CSD via electrical stimulation or to induce seizures via 4-aminopyridine application. We measured vascular dysfunction following CSDs and seizures in TBI animals using laser doppler flowmetry. We observed a more profound reduction in local cortical blood flow in TBI animals compared to healthy controls. CSD resulted in mitochondrial dysfunction and pathological signs of increased oxidative stress adjacent to the vasculature. We explored these findings further using electron microscopy and found that TBI and CSDs resulted in vascular morphological changes and mitochondrial cristae damage in astrocytes, pericytes and endothelial cells. Overall, we provide evidence that CSDs induce mitochondrial dysfunction, impaired cortical blood flow, and neurobehavioral deficits in the setting of TBI.

Recruiting pregnant people considering abortion: An analysis of differences in participant characteristics by recruitment site across online, abortion fund, and clinic-based sources.

OBJECTIVES: This study aimed to evaluate differences by recruitment method in the characteristics of participants who are considering abortion. STUDY DESIGN: Between June 2021 and April 2022, we recruited pregnant people considering abortion in Indiana from (1) online posts, (2) abortion funds, and (3) abortion clinics. We compared participant characteristics reported in an online survey by recruitment source. RESULTS: Compared to those recruited from clinics (n = 94), participants recruited online (n = 84) and through abortion funds (n = 239) were later in their pregnancy, were already parenting, received less formal education, struggled financially, and were more likely to be Black or African American, queer, transgender, or nonbinary. CONCLUSIONS: Recruitment from online sources and abortion funds reaches more people who face greater barriers to abortion care than recruitment from clinics. IMPLICATIONS: Augmenting clinic-based recruitment with online and abortion fund recruitment could capture a more complete sample of people considering abortion.