RESUMO
Cajal bodies are small nuclear organelles with a number of nuclear functions. Here we show that FLICE-associated huge protein (FLASH), originally described as a component of the apoptosis signaling pathway, is mainly localized in Cajal bodies and is essential for their structure. Reduction in FLASH expression by short hairpin RNA results in disruption of the normal architecture of the Cajal body and relocalization of its components. Because the function of FLASH in the apoptosis receptor signaling pathway has been strongly questioned, we have now identified a clear function for this protein.
Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , Corpos Enovelados/metabolismo , Animais , Proteínas Reguladoras de Apoptose/ultraestrutura , Proteínas de Ligação ao Cálcio/ultraestrutura , Corpos Enovelados/patologia , Corpos Enovelados/ultraestrutura , Regulação para Baixo/genética , Células HeLa , Humanos , Camundongos , Sinais de Localização Nuclear/metabolismo , Proteínas Nucleares/metabolismo , Biossíntese de Proteínas/genética , Transporte Proteico , Proteínas Recombinantes de Fusão/metabolismoRESUMO
Investigation of a small series of cases of typhoid fever infected in a river between 1963 and 1970 revealed that all were caused by a single source, a carrier of a rare phage type of Salmonella typhi. The contamination of the river resulted from an incorrect sewage connexion with a surface water drain outfall into the river.