Molecular evidence of Borrelia lusitaniae from questing Ixodes ticks in Algeria.

BACKGROUND: Ticks are hematophagous arthropods acting as important vectors for several microorganisms. Ticks have an important role in the epidemiology of numerous diseases from a public health standpoint. In Algeria, knowledge of tick-borne associated microorganisms is limited. This paper aimed to investigate the presence of microorganisms in Ixodes ticks in Ain Kerma El Tarf region. MATERIALS AND METHODS: Ticks were collected from the environment using the flagging method in El Hannachir, Ain kerma (El Tarf province), conserved in 70% ethanol, and morphologically identified. DNA was individually isolated from the ticks and screened for the presence of Piroplasmids and Hepatozoon spp., Borrelia spp., Spotted fever group Rickettsia, Bartonella spp., Mycoplasma spp., Anaplasmataceae, Anaplasma phagocytophilum, Francisella tularensis. RESULTS: Adult ticks of the genus Ixodes (n = 51) were collected. Due to their pronounced morphological resemblance, the sampled ticks are referenced as: I. ricinus/ I.inopinatus (n = 51, 26 males, 25 females). The following pathogens were identified in the tested ticks: Borrelia lusitaniae (n = 17; 33.33%), Rickettsia monacensis (n = 10; 19.60%), and R. helvetica (n = 5; 9.80%). In addition, five ticks (9.80%) were found to be coinfected with Borrelia lusitaniae and R. monacensis/ heletica. All ticks were negative for Piroplasmids and Hepatozoon spp., Bartonella spp., Mycoplasma spp., Anaplasmataceae, Anaplasma phagocytophilum and Francisella tularensis. CONCLUSION: Questing Ixodes ticks from Algeria are carriers of various pathogens, with Borrelia lusitaniae posing a potential risk in the country.

Italian peninsula as a hybridization zone of Ixodes inopinatus and I. ricinus and the prevalence of tick-borne pathogens in I. inopinatus, I. ricinus, and their hybrids.

BACKGROUND: Ixodes inopinatus was described from Spain on the basis of morphology and partial sequencing of 16S ribosomal DNA. However, several studies suggested that morphological differences between I. inopinatus and Ixodes ricinus are minimal and that 16S rDNA lacks the power to distinguish the two species. Furthermore, nuclear and mitochondrial markers indicated evidence of hybridization between I. inopinatus and I. ricinus. In this study, we tested our hypothesis on tick dispersal from North Africa to Southern Europe and determined the prevalence of selected tick-borne pathogens (TBPs) in I. inopinatus, I. ricinus, and their hybrids. METHODS: Ticks were collected in Italy and Algeria by flagging, identified by sequencing of partial TROSPA and COI genes, and screened for Borrelia burgdorferi s.l., B. miyamotoi, Rickettsia spp., and Anaplasma phagocytophilum by polymerase chain reaction and sequencing of specific markers. RESULTS: Out of the 380 ticks, in Italy, 92 were I. ricinus, 3 were I. inopinatus, and 136 were hybrids of the two species. All 149 ticks from Algeria were I. inopinatus. Overall, 60% of ticks were positive for at least one TBP. Borrelia burgdorferi s.l. was detected in 19.5% of ticks, and it was significantly more prevalent in Ixodes ticks from Algeria than in ticks from Italy. Prevalence of Rickettsia spotted fever group (SFG) was 51.1%, with significantly greater prevalence in ticks from Algeria than in ticks from Italy. Borrelia miyamotoi and A. phagocytophilum were detected in low prevalence (0.9% and 5.2%, respectively) and only in ticks from Italy. CONCLUSIONS: This study indicates that I. inopinatus is a dominant species in Algeria, while I. ricinus and hybrids were common in Italy. The higher prevalence of B. burgdorferi s.l. and Rickettsia SFG in I. inopinatus compared with that in I. ricinus might be due to geographical and ecological differences between these two tick species. The role of I. inopinatus in the epidemiology of TBPs needs further investigation in the Mediterranean Basin.

Molecular survey of flea-borne pathogens in fleas associated with carnivores from Algeria and an Artificial Neural Network-based risk analysis of flea-borne diseases.

As ectoparasites and efficient vectors of pathogens fleas constitute a source of nuisance for animals as well as a major issue for public health in Algeria. In this study, a molecular survey has been conducted to investigate the presence of pathogens in fleas infesting domestic and wild carnivores in the central north and eastern north and south of Algeria. The molecular screening that targeted Acanthocheilonema reconditum, Bartonella spp.,and Dipylidium caninum, was supplemented by a comprehensive analysis of risk factors related to flea-borne pathogens, drawing data from all documentation across multiple languages and sources from Morocco, Algeria, and Tunisia. In the current study, several Bartonella spp. 56/430 (13.02%) and Dipylidium caninum 3/430 (0.7%) were identified. The sequencing results revealed 5/23 (21.74%) B. clarridgeiae, 3/23 (13.04%) B. henselae, and 3/23 (13.04%) B. vinsonii. The two haplotypes, H1 and H2, of D. caninum were identified for the first time in North Africa. The results of the Artificial Neural Network risk analyses unveiled that the prevalence of pathogens and the presence of host generalist fleas as well as the vectorial competence are the most determinant risk factors of flea-borne diseases in Maghreb.

Bat Ectoparasites (Acari, Diptera, Hemiptera, Siphonaptera) in the Grand Maghreb (Algeria, Libya, Mauritania, Morocco and Tunisia): A Literature Review and New Data.

BACKGROUND: Arthropods parasites of bats play a crucial role in both ecological and public health contexts, as they have the potential to transmit zoonotic agents. The study aims to identify the distribution, and host-parasite associations of bat ectoparasites in the Grand Maghreb region (Algeria, Libya, Mauritania, Morocco and Tunisia), which has been largely understudied. METHODS: A thorough analysis of published records was conducted and we included our own field data. RESULTS: The checklist reveals a total of 43 ectoparasite species, encompassing a range of taxa. The list comprises 9 tick species, 11 mite species (including a chigger-mite), 11 bat fly species, 3 species of bugs, and 9 species of fleas. Extensive research efforts uncovered 141 host-parasite associations. Our data presents several new country records, documenting for the first time the presence of Carios vespertilionis and Raymondia huberi in Tunisia, Ixodes simplex and Spinturnix plecotinus in Algeria. CONCLUSION: By compiling and analysing available information, we have provided for the first time an up-to-date checklist of bat ectoparasites and their host associations in the region. This knowledge contributes to a better understanding of the epidemiological implications associated with bat ectoparasites, emphasizing their ecological and public health importance. The study's findings call for continued investigations and monitoring of bat ectoparasites to mitigate potential risks and safeguard both human and animal populations.

An overview of fleas (Siphonaptera) in wild and domestic mammals from Algeria with new data from the central north and south of the country.

Fleas (Siphonaptera) are medically and veterinary important ectoparasites known to infest a wide range of host species. This study presents a systematic review complemented by new data on the diversity of flea species and host associations in Algeria. 198 mammals were examined, including previously understudied hosts such as sheep, red foxes, fennec foxes, and golden African wolves. Of these animals, 108 (54.55%) were infested with fleas. Overall, 1.906 fleas belonging to seven species (Archaeopsylla erinacei s.l., Ctenocephalides felis, C. canis, Nosopsyllus fasciatus, Pariodontis riggenbachi, Pulex irritans, Xenopsylla cheopis) were identified, originating from seven provinces across Algeria. Statistical analysis techniques were employed to process the data, including descriptive statistics, statistical tests, and principal component analysis (PCA). This study reveals the diverse flea fauna in Algeria and their association with various host species, including wild and domestic animals. This comprehensive survey aimed to combine literature reviews, fieldwork, and statistical analysis to understand fleas' diversity and host associations. The findings contribute to the growing knowledge of flea ecology and host-parasite interactions, with implications for public health and veterinary practices in Algeria and potentially other regions with similar ecological characteristics.

First report of Crenosoma vulpis in Africa and Eucoleus aerophilus in Algeria.

Introduction: Crenosoma vulpis and Eucoleus aerophilus are widely distributed lungworms infecting carnivores, mainly red foxes, and are localized in the trachea, bronchi and bronchioles. There are no reports from Africa regarding the presence of C. vulpis. The aim of the present study was to report a co-infection with C. vulpis and E. aerophilus in a red fox from Algeria. Materials and methods: In January 2022, a road-killed male juvenile red fox (Vulpes vulpes) was collected from Bouhadjar-Tarf locality and was submitted for a complete parasitological necropsy. Detected nematodes were collected and preserved in ethanol for morphological and molecular identification. Tissue samples were also collected and analyzed by histopathological methods. Results: Collected nematodes were identified as a male C. vulpis and several Eucoleus aerophilus. The histological techniques of the lung tissue did not reveal the presence of any larvae, or lung inflammation. Conclusion: To the best of our knowledge, this is the first report of C. vulpis infecting a carnivore in this continent, highlighting the importance of further studies to update the geographical distribution of C. vulpis. E. aerophilus was first reported in Algeria. Red foxes are important spreaders of parasitic diseases. Further studies are required for a better understanding of its epidemiology across North Africa and other areas overlapping the range of the red fox.

Synopsis of the ticks of Algeria with new hosts and localities records.

BACKGROUND: Ticks are obligate hematophagous arthropods with a world-wide distribution that are extremely important not only in terms of human and animal health but also economically. In Algeria, information on tick species is scarce. METHODS: A systematic literature review was performed using online databases. The information extracted from the databases was was supplemented by information from an original study. Ticks were collected from various hosts and by flagging from January 2018 to December 2019. RESULTS: To date, in Algeria a total of 36 valid tick species belonging to two families have been recorded: (1) family Argasidae, with three Argas species and nine Ornithodoros species recorded; and (ii) family Ixodidae, with one Dermacentor species, three Haemaphysalis species, 10 Hyalomma species, four Ixodes species and six Rhipicephalus species recorded. The geographical distribution for each species was determined and listed. Eight new tick-host associations were recorded: four for Ixodes inopinatus sensu Estrada-Peña et al. 2014, one for Rhipicephalus bursa, one for R. turanicus, one for Hyalomma marginatum and one for Hy. lusitanicum. To our best knowledge, this study is the first to report the presence of I. inopinatus sensu Estrada-Peña et al. 2014 in Algeria. We also report here for the first time all tick species (Argasidae and Ixodidae) known to be present in Algeria. CONCLUSION: This article represents a tool for students and scientists who work in the field of ticks and provides important new data on the distribution of ticks in Algeria.

Fleas (Siphonaptera) of domestic and wild animals in extreme northeastern Algeria: first inventory, hosts, and medical and veterinary importance.

Fleas are an important member of the North African entomofauna. An understanding of the risks of flea-borne diseases to public and veterinary health can be gained with surveys of their abundance, distribution, and hosts. The aims of this study were to make an initial assessment of flea (Siphonaptera) species collected from a selected number of mammalian hosts in Algeria and debate their medical and veterinary importance. To do so, an entomological survey was conducted on several animal species (goats, dogs, cats, rabbits, hedgehogs, and mongooses) in six localities of El Tarf region located in extreme northeastern Algeria. During the survey, flea specimens were collected from hosts, stored in alcohol, and identified using a taxonomic key. More than 1,200 specimens were collected and identified; including four species: Ctenocephalides felis, Ctenocephalides canis, Pulex irritans, and Archaeopsylla erinacei (s.l.). Goats and dogs were the most infested animals, followed by cats and hedgehogs. Ctenocephalides felis was the most prevalent flea among all infested animals, with 631 collected specimens, followed by Pulex irritans with 433 samples. Overall, this study is an initial assessment of flea species recovered from selected common mammals in northeastern Algeria.

Subcutaneous ticks: a first report in a golden jackal, and their absence in non-canid carnivores.

BACKGROUND: Ticks are hematophagous arthropods which normally attach to the surface of the host's skin. Their aberrant presence in the subcutaneous tissue of a few carnivores, predominantly foxes, has been reported. However, there have been no reports of this phenomenon in other carnivores such as mustelids or golden jackals. Our aim was to investigate the host spectrum for this aberrant localization of ticks. METHODS: Between 2015 and 2020, a total of 198 carcasses of 12 species of carnivore were examined by parasitological necropsy. When a subcutaneous tick was found, the nodule was removed, carefully dissected, and stored in ethanol. The morphological identification of the subcutaneous tick was carried out to species level. RESULTS: A single subcutaneous tick was found in one carcass, that of a golden jackal (Canis aureus). The tick was identified as a female Ixodes ricinus. All the other carcasses were negative for the presence of subcutaneous ticks. CONCLUSION: To our knowledge, this is the first report of a subcutaneous tick in a golden jackal. This finding broadens the host spectrum of subcutaneous ticks, and reinforces the idea that, among carnivores, this phenomenon only occurs in canids.

Molecular evidence of bacteria in Melophagus ovinus sheep keds and Hippobosca equina forest flies collected from sheep and horses in northeastern Algeria.

The sheep ked, Melophagus ovinus, and the forest fly, Hippobosca equina, are parasitic dipteran insects of veterinary importance. As hematophagous insects, they might be considered as potential vectors of diseases which may be transmissible to humans and animals. The purpose of this study was to present initial primary data about these two species in Algeria. To do so, we conducted a molecular survey to detect the presence of bacterial DNA in flies collected in Algeria. A total of 712 flies including, 683 Melophagus ovinus and 29 Hippobosca equina were collected from two regions in northeastern Algeria. Monitoring the monthly kinetics of M. ovinus infestations showed something resembling annual activity, with a high prevalence in January (21.67%) and May (20.94%). Real-time quantitative PCR assays showed that for 311 tested flies, 126 were positive for the Bartonella spp. rRNA intergenic spacer gene and 77 were positive for Anaplasmataceae. A random selection of positive samples was submitted for sequencing. The DNA of Bartonella chomelii and Bartonella melophagi were amplified in, respectively, five and four H. equina. 25 M. ovinus positive samples were infected by Bartonella melophagi. Amplification and sequencing of the Anaplasma spp. 23S rRNA gene revealed that both species were infected by Wolbachia sp. which had previously been detected in Cimex lectularius bed bugs. Overall, this study expanded knowledge about bacteria present in parasitic flies of domestic animals in Algeria.